Influence of different application protocols of the universal adhesive system on microleakage of Class V restorations. In vitro study




Dentin-bonding agents; Dental leakage; Composite resins.


The aim of the present study was to evaluate the influence of different application modes of the universal adhesive system on the marginal microleakage of class V composite resin restorations. For this, 30 bovine incisors were selected, divided into three groups (n=10): Group CT – total etch; Group AC – self-etching and Group CSE – selective enamel etching. The adhesive used was a universal adhesive (Ambar Universal APS, FGM), applied according to the groups. After cavity preparation and application of the adhesive system, they were restored with nanoparticulate composite resin using the incremental technique. After 24 hours, immersed in distilled water, all specimens were prepared and submitted to microleakage test for 24 hours in a 2% methylene blue solution. Then, they were sectioned in the buccolingual direction and evaluated for marginal leakage. The results obtained were submitted to statistical analysis, where no statistically significant difference was observed between the groups. Thus, according to the results of this work, it can be concluded that the universal adhesive can be used in a self-etching form, with no need for pre-conditioning with phosphoric acid.

Author Biographies

Stéphane Vanelli da Silva, Universidade Estadual do Oeste do Paraná

Curso de Odontologia

Cassia Thaís Iurkiv Zanatta, Universidade Estadual do Oeste do Paraná

Curso de Odontologia

Stephanie Bonamin, Universidade Estadual do Oeste do Paraná

Curso de Odontologia

Veridiana Camilotti, Universidade Estadual do Oeste do Paraná

Curso de Odontologia

Márcio José Mendonça, Universidade Estadual do Oeste do Paraná

Curso de Odontologia


Amaral, M., Belli, R., Cesar, P. F., Valandro, L. F., Petschelt, A., & Lohbauer, U. (2014). The potential of novel primers and universal adhesives to bond to zirconia. Journal of Dentistry, 42(1), 90–98.

Atalay, C., Ozgunaltay, G., & Yazici, A. R. (2020). Thirty-six-month clinical evaluation of different adhesive strategies of a universal adhesive. Clinical Oral Investigations, 24(4), 1569–1578.

Chandra, P. V. R., Harikumar, V., Ramkiran, D., Krishna, M. J. N., & Gouda, M. V. (2013). Microleakage of class V resin composites using various self-etching adhesives: An in vitro study. The Journal of Contemporary Dental Practice, 14(1), 51–55.

Chen, C., Niu, L.‑N., Xie, H., Zhang, Z.‑Y., Zhou, L.‑Q., Jiao, K., Chen, J.‑H., Pashley, D. H [D. H.], & Tay, F. R. (2015). Bonding of universal adhesives to dentine--Old wine in new bottles? Journal of Dentistry, 43(5), 525–536.

Da Rosa, W. L. d. O., Piva, E., & Da Silva, A. F. (2015). Bond strength of universal adhesives: A systematic review and meta-analysis. Journal of Dentistry, 43(7), 765–776.

Erickson, R. L., Barkmeier, W. W., & Latta, M. A. (2009). The role of etching in bonding to enamel: A comparison of self-etching and etch-and-rinse adhesive systems. Dental Materials : Official Publication of the Academy of Dental Materials, 25(11), 1459–1467.

Frankenberger, R., Lohbauer, U., Roggendorf, M. J., Naumann, M., & Taschner, M. (2008). Selective enamel etching reconsidered: Better than etch-and-rinse and self-etch? The Journal of Adhesive Dentistry, 10(5), 339–344.

Going, R. E. (1972). Microleakage around dental restorations: A summarizing review. Journal of the American Dental Association (1939), 84(6), 1349–1357.

Guéders, A. M., Charpentier, J. F., Albert, A. I., & Geerts, S. O. (2006). Microleakage after thermocycling of 4 etch and rinse and 3 self-etch adhesives with and without a flowable composite lining. Operative Dentistry, 31(4), 450–455.

Gupta, A., Tavane, P., Gupta, P. K., Tejolatha, B., Lakhani, A. A., Tiwari, R., Kashyap, S., & Garg, G. (2017). Evaluation of Microleakage with Total Etch, Self Etch and Universal Adhesive Systems in Class V Restorations: An In vitro Study. Journal of Clinical and Diagnostic Research : JCDR, 11(4), ZC53-ZC56.

Hanabusa, M., Mine, A., Kuboki, T., Momoi, Y., van Ende, A., van Meerbeek, B [Bart], & Munck, J. de [Jan] (2012). Bonding effectiveness of a new 'multi-mode' adhesive to enamel and dentine. Journal of Dentistry, 40(6), 475–484.

Jordehi, A. Y., Shahabi, M. S., & Akbari, A. (2019). Comparison of self-adhering flowable composite microleakage with several types of bonding agent in class V cavity restoration. Dental Research Journal, 16(4), 257–263.

Karaman, E., & Güler, E. (2016). Marginal Adaptation of Class V Restorations with a New Universal Adhesive. The International Journal of Periodontics & Restorative Dentistry, 36(1), 125–130.

Kermanshah, H., & Khorsandian, H. (2017). Comparison of microleakage of Scotchbond™ Universal Adhesive with methacrylate resin in Class V restorations by two methods: Swept source optical coherence tomography and dye penetration. Dental Research Journal, 14(4), 272–281.

Lawson, N. C., Robles, A., Fu, C.‑C., Lin, C. P., Sawlani, K., & Burgess, J. O. (2015). Two-year clinical trial of a universal adhesive in total-etch and self-etch mode in non-carious cervical lesions. Journal of Dentistry, 43(10), 1229–1234.

Loguercio, A. D [Alessandro Dourado], Stanislawczuk, R., Mena-Serrano, A., & Reis, A [Alessandra] (2011). Effect of 3-year water storage on the performance of one-step self-etch adhesives applied actively on dentine. Journal of Dentistry, 39(8), 578–587.

Lohbauer, U., Rahiotis, C., Krämer, N., Petschelt, A., & Eliades, G. (2005). The effect of different light-curing units on fatigue behavior and degree of conversion of a resin composite. Dental Materials, 21(7), 608–615.

Manfroi, F. B., Ethur, E. M., Somacal, D. C., Paludo, T., & Spohr, A. M. (2020). Effect of Cariogenic Challenge on the Degradation of Adhesive-Dentin Interfaces. Brazilian Dental Journal, 31(2), 179–185.

Marchesi, G., Frassetto, A., Mazzoni, A., Apolonio, F., Diolosà, M., Cadenaro, M., Di Lenarda, R., Pashley, D. H [David H.], Tay, F., & Breschi, L. (2014). Adhesive performance of a multi-mode adhesive system: 1-year in vitro study. Journal of Dentistry, 42(5), 603–612.

Mousavinasab, S. M., Atai, M., & Alavi, B. (2011). To compare the microleakage among experimental adhesives containing nanoclay fillers after the storages of 24 hours and 6 months. The Open Dentistry Journal, 5(1), 52–57.

Munck, J. de [J.], van Landuyt, K., Peumans, M [M.], Poitevin, A., Lambrechts, P., Braem, M., & van Meerbeek, B [B.] (2005). A critical review of the durability of adhesion to tooth tissue: Methods and results. Journal of Dental Research, 84(2), 118–132.

Muñoz, M. A [M. A.], Luque-Martinez, I., Malaquias, P., Hass, V [V.], Reis, A [A.], Campanha, N. H., & Loguercio, A. D [A. D.] (2015). In vitro longevity of bonding properties of universal adhesives to dentin. Operative Dentistry, 40(3), 282–292.

Muñoz, M. A [Miguel Angel], Luque, I., Hass, V [Viviane], Reis, A [Alessandra], Loguercio, A. D [Alessandro Dourado], & Bombarda, N. H. C. (2013). Immediate bonding properties of universal adhesives to dentine. Journal of Dentistry, 41(5), 404–411.

Patanjali, S., Arora, A., Arya, A., & Grewal, M. S. (2019). An In Vitro Study of Effect of Beveling of Enamel on Microleakage and Shear Bond Strength of Adhesive Systems in Primary and Permanent Teeth. International Journal of Clinical Pediatric Dentistry, 12(3), 205–210.

Perdigão, J [J.], Kose, C., Mena-Serrano, A. P., Paula, E. A. de, Tay, L. Y., Reis, A [A.], & Loguercio, A. D [A. D.] (2014). A new universal simplified adhesive: 18-month clinical evaluation. Operative Dentistry, 39(2), 113–127.

Perdigão, J [Jorge], Sezinando, A., & Monteiro, P. C. (2012). Laboratory bonding ability of a multi-purpose dentin adhesive. American Journal of Dentistry, 25(3), 153–158.

Peumans, M [M.], Munck, J. de [J.], van Landuyt, K. L., Poitevin, A., Lambrechts, P., & van Meerbeek, B [B.] (2010). Eight-year clinical evaluation of a 2-step self-etch adhesive with and without selective enamel etching. Dental Materials : Official Publication of the Academy of Dental Materials, 26(12), 1176–1184.

Pleffken, P. R., Almeida Lourenço, A. P. de, Torres, C. R., & Bühler Borges, A. (2011). Influence of application methods of self-etching adhesive systems on adhesive bond strength to dentin. The Journal of Adhesive Dentistry, 13(6), 517–525.

Pouyanfar, H., Tabaii, E. S., Aghazadeh, S., Nobari, S. P. T. N., & Imani, M. M. (2018). Microtensile Bond Strength of Composite to Enamel Using Universal Adhesive with/without Acid Etching Compared To Etch and Rinse and Self-Etch Bonding Agents. Open Access Macedonian Journal of Medical Sciences, 6(11), 2186–2192.

Rengo, C., Goracci, C., Juloski, J., Chieffi, N., Giovannetti, A., Vichi, A., & Ferrari, M. (2012). Influence of phosphoric acid etching on microleakage of a self-etch adhesive and a self-adhering composite. Australian Dental Journal, 57(2), 220–226.

Shafiei, F., Dehghani, Z., & Jowkar, Z. (2021). The influence of the operator's experience on the microleakage of two universal adhesives. Clinical and Experimental Dental Research. Advance online publication.

Taylor, M. J., & Lynch, E. (1992). Microleakage. Journal of Dentistry, 20(1), 3–10.

van Meerbeek, B [Bart], Yoshihara, K [Kumiko], van Landuyt, K [Kirsten], Yoshida, Y [Yasuhiro], & Peumans, M [Marleen] (2020). From Buonocore's Pioneering Acid-Etch Technique to Self-Adhering Restoratives. A Status Perspective of Rapidly Advancing Dental Adhesive Technology. The Journal of Adhesive Dentistry, 22(1), 7–34.

Wagner, A., Wendler, M., Petschelt, A., Belli, R., & Lohbauer, U. (2014). Bonding performance of universal adhesives in different etching modes. Journal of Dentistry, 42(7), 800–807.

Yoshida, Y [Y.], Yoshihara, K [K.], Nagaoka, N., Hayakawa, S., Torii, Y., Ogawa, T., Osaka, A., & van Meerbeek, B [B.] (2012). Self-assembled Nano-layering at the Adhesive interface. Journal of Dental Research, 91(4), 376–381.



How to Cite

SILVA, S. V. da; ZANATTA, C. T. I.; BONAMIN, S.; CAMILOTTI, V.; MENDONÇA, M. J.; UEDA, J. K. Influence of different application protocols of the universal adhesive system on microleakage of Class V restorations. In vitro study. Research, Society and Development, [S. l.], v. 11, n. 9, p. e25511926081, 2022. DOI: 10.33448/rsd-v11i9.26081. Disponível em: Acesso em: 13 aug. 2022.



Health Sciences