Oxidative stress in SH-SY5Y cells on exposure to hydrogen peroxide
DOI:
https://doi.org/10.33448/rsd-v11i3.26474Keywords:
Oxidative stress; Hydrogen peroxide; Neuroblastoma.Abstract
Oxidative stress is obtained through the formation of reactive oxygen species, which are normally produced by cell metabolism, but when produced in excess, the cell loses its ability to defend and repair, leading to damage tobiomolecules (DNA, lipids, proteins, etc). When these damages are not repaired, they end up compromising the cell's functioning, leading to death by apoptosis or necrosis. Oxidative stress is involved in neural cell death, which is a major cause of neurodegenerative diseases, including Alzheimer and Parkinson's disease. The aim of this study is to verify the action of hydrogen peroxide as a model of oxidative stress in human neuroblastoma cells. In order to analyze from which concentration a significant stress occurs in the cells and together, verify each concentration at different times of action in the cells. The experiments performed were the analysis of mitochondrial activity obtained through the MTT test, cell viability test using the Trypan blue assay, obtaining quantitative and qualitative data. It is possible to observe that from the concentration of 0.2 mM in the time of 2 hours, there is a significant decrease in the number of cells in relation to the control group, therefore, with the increase of the other concentrations in relation to time, more damage occurs in the cells.
References
Bader, R., Ibrahim, J. N., Moussa, M., Mourad, A., Azoury, J., Azoury, J., & Alaaeddine, N. (2020). In vitro effect of autologous platelet‐rich plasma on H2O2‐induced oxidative stress in human spermatozoa. Andrology, 8(1), 191-200.
Barbosa, K. B. F., Costa, N. M. B., Alfenas, R. D. C. G., De Paula, S. O., Minim, V. P. R., & Bressan, J. (2010). Oxidative stress: concept, implications and modulating factors. Rev Nutr, 23(4), 629-43.
Barreiros, A. L., David, J. M., & David, J. P. (2006). Estresse oxidativo: relação entre geração de espécies reativas e defesa do organismo. Química nova, 29(1), 113-123.
Feng, C., Luo, T., Zhang, S., Liu, K., Zhang, Y., Luo, Y., & Ge, P. (2016). Lycopene protects human SH SY5Y neuroblastoma cells against hydrogen peroxide induced death via inhibition of oxidative stress and mitochondria associated apoptotic pathways. Molecular medicine reports, 13(5), 4205-4214.
Ferreira, A. L. A., & Matsubara, L. S. (1997). Radicais livres: conceitos, doenças relacionadas, sistema de defesa e estresse oxidativo. Revista da associação médica brasileira, 43(1), 61-68.
Gille, J. J. P., & Joenje, H. (1992). Cell culture models for oxidative stress: superoxide and hydrogen peroxide versus normobaric hyperoxia. Mutation Research/DNAging, 275(3-6), 405-414.
Klein, J. A., & Ackerman, S. L. (2003). Oxidative stress, cell cycle, and neurodegeneration. The Journal of clinical investigation, 111(6), 785-793.
Loh, S. H., Tsai, C. S., Tsai, Y., Chen, W. H., Hong, G. J., Wei, J., & Lin, C. I. (2002). Hydrogen peroxide-induced intracellular acidosis and electromechanical inhibition in the diseased human ventricular myocardium. European journal of pharmacology, 443(1-3), 169-177.
McCord, J. M. (2000). The evolution of free radicals and oxidative stress. The American journal of medicine, 108(8), 652-659.
Nirmaladevi, D., Venkataramana, M., Chandranayaka, S., Ramesha, A., Jameel, N. M., & Srinivas, C. (2014). Neuroprotective effects of bikaverin on H2O2-induced oxidative stress mediated neuronal damage in SH-SY5Y cell line. Cellular and molecular neurobiology, 34(7), 973-985.
Park, H. R., Lee, H., Park, H., Jeon, J. W., Cho, W. K., & Ma, J. Y. (2015). Neuroprotective effects of Liriope platyphylla extract against hydrogen peroxide-induced cytotoxicity in human neuroblastoma SH-SY5Y cells. BMC Complementary and Alternative Medicine, 15(1), 1-11.
Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa cientifica. UFSM.
Repetto, G., Del Peso, A., & Zurita, J. L. (2008). Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nature protocols, 3(7), 1125-1131.
Rossato, R. C., Pinto, J. C., de Oliveira Moraes, C. D. G., de Oliveira Moraes, J. T. G., Salles, G. N., & Soares, C. P. (2020). Efeitos neuroprotetores da hidrocortisona em linhagem de Neuroblastoma de Murino (NEURO-2A) sob estresse oxidativo. Revista Univap, 26(52), 93-106.
Ruffels, J., Griffin, M., & Dickenson, J. M. (2004). Activation of ERK1/2, JNK and PKB by hydrogen peroxide in human SH-SY5Y neuroblastoma cells: role of ERK1/2 in H2O2-induced cell death. European journal of pharmacology, 483(2-3), 163-173.
Salles, G. N., Pereira, F. A. D. S., Pacheco-Soares, C., Marciano, F. R., Hölscher, C., Webster, T. J., & Lobo, A. O. (2017). A novel bioresorbable device as a controlled release system for protecting cells from oxidative stress from Alzheimer’s Disease. Molecular neurobiology, 54(9), 6827-6838.
Seetapun, S., Yaoling, J., Wang, Y., & Zhu, Y. Z. (2013). Neuroprotective effect of Danshensu derivatives as anti-ischaemia agents on SH-SY5Y cells and rat brain. Bioscience Reports, 33(4).
Tetich, M., Kutner, A., Leskiewicz, M., Budziszewska, B., & Lasoń, W. (2004). Neuroprotective effects of (24R)-1, 24-dihydroxycholecalciferol in human neuroblastoma SH-SY5Y cell line. The Journal of steroid biochemistry and molecular biology, 89, 365-370.
Uberti, D., Piccioni, L., Colzi, A., Bravi, D., Canonico, P. L., & Memo, M. (2002). Pergolide protects SH-SY5Y cells against neurodegeneration induced by H2O2. European journal of pharmacology, 434(1-2), 17-20.
Uttara, B., Singh, A. V., Zamboni, P., & Mahajan, R. (2009). Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Current neuropharmacology, 7(1), 65-74.
van Muiswinkel, F. L., & Kuiperij, H. B. (2005). The Nrf2-ARE Signalling pathway: promising drug target to combat oxidative stress in neurodegenerative disorders. Current Drug Targets-CNS & Neurological Disorders, 4(3), 267-281.
Zabeu, A. M. C., Carvalho, I. C. S., Pacheco-Soares, C., & da Silva, N. S. (2021). Biomodulatory effect of low intensity laser (830 nm.) in neural model 9L/lacZ. Research, Society and Development, 10(8), e11310817025-e11310817025.
Zhang, X., Wang, L., Lu, H., Zong, Z., Chen, Z., Li, Y., & Li, Y. (2020). Preservation of hydrogen peroxide-induced oxidative damage in HepG-2 cells by rice protein hydrolysates pretreated with electron beams. Scientific Reports, 10(1), 1-11.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Antonieta Marques Caldeira Zabeu; Rafaella Rossato ; Cristina Pacheco Soares; Newton Soares da Silva
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
