Isolation of Gram-negative bacteria from manguezal sediment samples is São Luís, Maranhão
DOI:
https://doi.org/10.33448/rsd-v11i3.26483Keywords:
Sediment; Mangrove; Drug resistence; Physico-chemical.Abstract
Mangrove sediments are very moist and rich in decomposition organic matter, formed by a series of species resistant to tidal flow. This coastal ecosystem is formed by a series of species resistant to tidal flow, with soil containing a rich environment that limits the diversity of biomes. The objective of this study was the identification of microorganisms and the physicochemical characterization in sediment samples from two points of a mangrove located near a hospital on the island of São Luís, Maranhão. Two sediment samples were collected in appropriate tubes, stored and sent to the Environmental Microbiology Laboratory at CEUMA University. In the laboratory, aliquots were removed for the physical-chemical characteristics using a methodology as recommended by the Brazilian Agricultural Research Company (EMBRAPA), tracking characteristics such as granulometry, organic matter, organic carbon, nitrate and nitrite, total phosphorous and pH. The soil was characterized as organic, rich in nitrate, nitrite and mineral salts, with pH (6.3), characteristic of the mangrove ecosystem. Isolation, identification of MALDI-TOF isolates (Matrix-assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectrometry) and antimicrobial susceptibility test used Kirby-Bauer methodology were performed. Gram-negative bacteria were identified, of the genus Ochrobactrum and thermotolerant species, such as Escherichia coli, which are considered pathogens that are indicators of lack of basic sanitation and that presented multiresistance in vitro to the antibiotics tested. Our study strengthens the idea of further research to evaluate the diversity of microorganisms present in mangroves to encourage and warn about the preservation and sanitary control of mangrove ecosystems.
References
Alonso, C. A., Kwabugge, Y. A., Anyanwu, M. U., Torres, C., & Chah, K. F. (2017). Diversity of Ochrobactrum species in food animals, antibiotic resistance phenotypes and polymorphisms in the blaOCH gene. FEMS Microbiology Letters, 364(17). https://doi.org/10.1093/femsle/fnx178
Araujo De Brito, M., Benedito, ;, & Cordeiro, C. (n.d.). Necessidade de novos antibióticos The need of new antibiotics. 2012. Retrieved February 1, 2022, from www.imshealth.com/portal/site/ims
Arruda, J. M., Siqueira, C., Souza, V., Silva, F., Silva, L., Santos, J., Cipriano, K., Dias, D., & Faro, L. (2019). Bibliographic review of Beta-lactam antibiotics. Revista Saúde Em Foco, 11, 982–995. http://www.icb.usp.br/bmm/mariojac/index.php?option=com_content&view=article&id=47&I
Barbosa, S. C. de B. 1988-. (2012). Determinação de resíduos de pesticidas em produtos de origem vegetal por GC-ECD. https://repositorio.ul.pt/handle/10451/7448
Berrêdo, J. F., Costa, M. L., & Progene, M. D. P. S. (2008). Efeitos das variações sazonais do clima tropical úmido sobre as águas e sedimentos de manguezais do estuário do rio Marapanim, costa nordeste do Estado do Pará. Acta Amazonica, 38(3), 473–482. https://doi.org/10.1590/S0044-59672008000300012
Bezerra, D. da S. (2008). O Ecossistema Manguezal em Meio Urbano no Contexto de Políticas Públicas de Uso e Ocupação do Solo na Bacia do Rio Anil , São Luis , Maranhão. [Universidade Federal do Maranhão]. http://tedebc.ufma.br:8080/jspui/handle/tede/1077
Blainski, É., Tormena, C. A., Guimarães, R. M. L., & Nanni, M. R. (2012). Influence of soil cover on the soil physical quality of an Oxisol in no-tillage. Revista Brasileira de Ciencia Do Solo, 36(1), 79–87. https://doi.org/10.1590/s0100-06832012000100009
Bryan-Brown, D. N., Connolly, R. M., Richards, D. R., Adame, F., Friess, D. A., & Brown, C. J. (2020). Global trends in mangrove forest fragmentation. Scientific Reports, 10(1). https://doi.org/10.1038/S41598-020-63880-1
Bush, K., & Bradford, P. A. (2016). β-Lactams and β-Lactamase Inhibitors: An Overview. Cold Spring Harbor Perspectives in Medicine, 6(8). https://doi.org/10.1101/CSHPERSPECT.A025247
Campos, M. C. C., Ribeiro, M. R., Júnior, V. S. de S., Filho, R. R., & Almeida, M. C. (2012). Topossequência de solos na transição campos naturais-floresta na região de Humaitá, Amazonas. Acta Amazonica, 42(3), 387–398. https://doi.org/10.1590/S0044-59672012000300011
Campos, M. L., Da Silva, F. N., Furtini Neto, A. E., Guimarães Guilherme, L. R., Marques, J. J., & Antunes, A. S. (2005). Determination of cadmium, copper, chromium, nickel, lead and zinc in rock phosphates. Pesquisa Agropecuaria Brasileira, 40(4), 361–367. https://doi.org/10.1590/s0100-204x2005000400007
Campos, M. L., Silva, F. N. da, Furtini Neto, A. E., Guilherme, L. R. G., Marques, J. J., & Antunes, A. S. (2005). Determinação de cádmio, cobre, cromo, níquel, chumbo e zinco em fosfatos de rocha. Pesquisa Agropecuária Brasileira, 40(4), 361–367. https://doi.org/10.1590/s0100-204x2005000400007
Celeri, M. J., Mendes, L. E. T., Lima, R. M. B. de F., & Vasconcelos, T. da R. (2019). A cidade, o mangue e os resíduos sólidos: estudo de caso do Manguezal Vinhais, São Luís – MA/ The city, the mangue and the solid waste:The Vinhais Mangrove case in the city of São Luís -MA. Geografia Em Atos (Online), 3(10), 163–186. https://doi.org/10.35416/geoatos.v3i10.5710
Chen, Y. S., Wong, W. W., Fung, C. P., Yu, K. W., & Liu, C. Y. (2002). Clinical features and antimicrobial susceptibility trends in Citrobacter freundii bacteremia. Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi, 35(2), 109–114. https://europepmc.org/article/med/12099331
Coutinho, H. D. M., Costa, J. G. M., Lima, E. O., Falcão-Silva, V. S., & Siqueira, J. P. (2009). Herbal therapy associated with antibiotic therapy: Potentiation of the antibiotic activity against methicillin - Resistant Staphylococcus aureus by Turnera ulmifolia L. BMC Complementary and Alternative Medicine, 9. https://doi.org/10.1186/1472-6882-9-13
Cuzzuol, G. R. F., & Rocha, A. C. (2012). Interação do regime hídrico com as relações nutricionais em ecossistema manguezal. Acta Botanica Brasilica, 26(1), 11–19. https://doi.org/10.1590/S0102-33062012000100003
de Lima Grisi, T. C. S., & Gorlach-Lira, K. (2010). The abundance of some pathogenic bacteria in mangrove habitats of Paraiba do Norte estuary and crabmeat contamination of mangrove crab Ucides cordatus. Brazilian Archives of Biology and Technology, 53(1), 227–234. https://doi.org/10.1590/S1516-89132010000100028
Dias, J. C., & Hofer, E. (1985). Bactérias gram negativas resistentes a antimicrobianos em alimentos. Memórias Do Instituto Oswaldo Cruz, 80(4), 411–421. https://doi.org/10.1590/S0074-02761985000400006
Donagem, G. K., Campos, D. V. B. de, Calderano, S. B., Teixeira, W. G., & Viana, J. H. M. (2011). Manual de métodos de análise de solo. - Portal Embrapa. https://www.embrapa.br/busca-de-publicacoes/-/publicacao/990374/manual-de-metodos-de-analise-de-solo
Dubey, S. K., Tripathi, A. K., & Upadhyay, S. N. (2006). Exploration of soil bacterial communities for their potential as bioresource. Bioresource Technology, 97(17), 2217–2224. https://doi.org/10.1016/J.BIORTECH.2005.06.008
Fonsêca, I. L. A., & Mochel, F. R. (2016). Fitomassa aérea de um manguezal no estuário do rio dos cachorros, são luís, maranhão, brasil. In BOLETIM DO laboratório de hidrobiologia Bol. Lab. Hidrobiol (Vol. 26, Issue 1, pp. 17–25). http://www.periodicoseletronicos.ufma.br/index.php/blabohidro/article/view/5441
Gomes, C. de M. T. [UNESP]. (2001). Degradação ambiental urbana e qualidade de vida nas áreas de manguezais ocupadas por palafitas em São Luís - MA. Aleph, 73 f. : il. https://repositorio.unesp.br/handle/11449/89827
Guimarães, D. O., Da Silva Momesso, L., & Pupo, M. T. (2010). Antibióticos: Importância terapêutica e perspectivas para a descoberta e desenvolvimento de novos agentes. In Quimica Nova (Vol. 33, Issue 3, pp. 667–679). Sociedade Brasileira de Química. https://doi.org/10.1590/S0100-40422010000300035
Lu, S. Y., Zhang, Y. L., Geng, S. N., Li, T. Y., Ye, Z. M., Zhang, D. S., Zou, F., & Zhou, H. W. (2010). High diversity of extended-spectrum beta-lactamase-producing bacteria in an urban river sediment habitat. Applied and Environmental Microbiology, 76(17), 5972–5976. https://doi.org/10.1128/AEM.00711-10/SUPPL_FILE/SUPPLEMENT_TABLE_1.ZIP
Lupo, A., Papp-Wallace, K. M., Sendi, P., Bonomo, R. A., & Endimiani, A. (2013). Non-phenotypic tests to detect and characterize antibiotic resistance mechanisms in Enterobacteriaceae. Diagnostic Microbiology and Infectious Disease, 77(3), 179–194. https://doi.org/10.1016/J.DIAGMICROBIO.2013.06.001
Machado, E. C., Leal, C. D., Coelho, B. L., Chernicharo, C. A. de L., & de Araújo, J. C. (2020). Detecção e quantificação de bactérias resistentes aos antibióticos ampicilina e cloranfenicol em estações de tratamento de esgoto doméstico. Engenharia Sanitaria e Ambiental, 25(6), 847–857. https://doi.org/10.1590/S1413-4152202020180001
Martins Terceiro, A., Jethro, J., & Santos, S. (2013). Caracterização da sociedade, economia e meio ambiente costeiro atuante à exploração dos manguezais no estado do maranhão. Revista de Administração e Negócios Da Amazônia, 5(3), 94–111.
Metila, V. De. (2007). Análise microbiológica do caranguejo Uçá, Ucides cordatus (Linnaeus, 1963), como bioindicador ambiental dos manguezais dos Rio Paciência, Ilha de São Luís-MA. Ecologia. http://www.ufsc.br/.
Moura-Fé, M. M., Albuquerque, A. G. B. M., Freitas, E. M. N., & Barbosa, W. R. (2015). A Proteção do Ecossistema Manguezal pela Legislação Ambiental Brasileira. GEOgraphia, 17(33), 126–153. https://doi.org/10.22409/GEOGRAPHIA2015.V17I33.A13700
Pereira, E. B., Barros, F. D., & Hollanda, L. M. de. (2019). Bioprospecção de bactérias produtoras de antimicrobianos isoladas dos solos de diferentes manguezais do estado de Sergipe. Semana de Pesquisa e Extensão Da Universidade Tiradentes - SEMPESq-SEMEX, 0(21). https://eventos.set.edu.br/sempesq/article/view/12768
Rocha, L. L. (2008). Estudo de comunidades bacterianas de solos de manguezal da Barra Grande, Icapui - Ce e seleção de cepas com potencial para degradar hidrocarbonetos. http://www.repositorio.ufc.br/handle/riufc/4923
Silva, M. R. C., Silva, L. V. da, Barreto, L. N., Rodrigues, E. H. C., Miranda, R. de C. M. de, Bezerra, D. S., & Pereira, D. C. A. (2017). Qualidade da Água da Bacia do Rio Pindaré, nos trechos correspondentes aos Municípios de Pindaré- Mirim, Tufilândia e Alto Alegre no Estado do Maranhão. Águas Subterrâneas, 31(4), 347–354. https://doi.org/10.14295/RAS.V31I4.28929
Silva, W. D. E. M. (2015). Metais pesados em solos de manguezais em estuários da bacia Paraíba, Nordeste do Brasil.
Sivagami, K., Vignesh, V. J., Srinivasan, R., Divyapriya, G., & Nambi, I. M. (2018). Antibiotic usage, residues and resistance genes from food animals to human and environment: An Indian scenario. Undefined, 8(1). https://doi.org/10.1016/J.JECE.2018.02.029
Soares Júnior, F. L., Fernando, ;, Andreote, D., João, ;, Da Silva, L., Júlio, ;, De Queiroz, F., & De Melo, I. S. (2016). Avaliação da resistência a antibióticos de bactérias isoladas da água de viveiros de camarão. http://www.alice.cnptia.embrapa.br/handle/doc/15750
Thurm, V., & Gericke, B. (1994). Identification of infant food as a vehicle in a nosocomial outbreak of Citrobacter freundii: epidemiological subtyping by allozyme, whole-cell protein and antibiotic resistance. Journal of Applied Bacteriology, 76(6), 553–558. https://doi.org/10.1111/J.1365-2672.1994.TB01652.X
Von Wintersdorff, C. J. H., Penders, J., Van Niekerk, J. M., Mills, N. D., Majumder, S., Van Alphen, L. B., Savelkoul, P. H. M., & Wolffs, P. F. G. (2016). Dissemination of antimicrobial resistance in microbial ecosystems through horizontal gene transfer. Frontiers in Microbiology, 7(FEB), 173. https://doi.org/10.3389/FMICB.2016.00173/BIBTEX
Zhao, H., Yan, B., Mo, X., Li, P., Li, B., Li, Q., Li, N., Mo, S., Ou, Q., Shen, P., Wu, B., & Jiang, C. (2019). Prevalence and proliferation of antibiotic resistance genes in the subtropical mangrove wetland ecosystem of South China Sea. MicrobiologyOpen, 8(11), 871. https://doi.org/10.1002/MBO3.871
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Verônica Duarte da Silva ; Yuri Nascimento Fróes; Joveliane de Melo Monteiro ; Neemias Muniz de Souza; Neuriane Silva Lima; Leila Cristina Almeida de Sousa; Anna Regina Lanner de Moura; Joicy Cortez de Sá Sousa; Fabrício Brito Silva; Darlan Ferreira da Silva; Andrea de Souza Monteiro; Maria Raimunda Chagas Silva
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
