Perfil bioquímico e significado clínico de genes da via MAPK/KINASE no diagnóstico de neoplasias de tireoide
DOI:
https://doi.org/10.33448/rsd-v11i3.26671Palavras-chave:
Tireoide; Expressão gênica; Marcador molecular; MAPK/KINASE; Espectroscopia; FTIR.Resumo
As neoplasias da tireoide são os principais tipos de malignidade endócrina, apresentando incidência aumentada nos últimos anos. O diagnóstico de rotina pode apresentar resultados inconclusivos, levando à necessidade de utilização de técnicas adicionais mais precisas, tais como a análise molecular. Portanto, é essencial a busca de marcadores moleculares para o diagnóstico dessas neoplasias e seus diferentes tipos histológicos. O objetivo deste estudo foi identificar marcadores moleculares diagnósticos para carcinoma papilífero de tireoide (CPT) e lesões de bócio. Para isso, a expressão de genes pertencentes à via MAPK/KNASE foi avaliada pela técnica de RT-qPCR. Adicionalmente, perfis bioquímicos completos das amostras foram obtidos por meio da espectroscopia de infravermelho com transformada de Fourier (FTIR). Os resultados do RT-qPCR sugerem que os genes FOS, JUN, MAP2K6, CCNA1, SFN têm o potencial de serem marcadores tumorais de lesões da tireoide, e os genes MAP2K6, CCNA1, SFN têm ainda o potencial de distinguir amostras de CPT de outras lesões de tireoide. Os resultados do FTIR mostraram que as lesões de CPT podem ser diferenciadas de tecidos normais e benignos com 95,83% de eficiência; sendo as alterações nos ácidos nucleicos o principal fator de classificação. No geral, os resultados sugerem o potencial da análise molecular e do FTIR no diagnóstico do câncer de tireoide.
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Copyright (c) 2022 Joyce Nascimento Santos; Raissa Monteiro da Silva; Tanmoy Tapobrata Bhattacharjee; Marco Aurélio Vamondes Kulcsar; Miyuki Uno; Roger Chammas; Renata de Azevedo Canevari
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