Effects of Brazilian green propolis glycolic solutions against bacterial biofilm on elastomers used in maxillofacial prostheses





Biofilm; Dental materials; Maxillofacial prosthesis; Propolis; Staphylococcus aureus.


Maxillofacial prostheses are among the modalities of prosthetic rehabilitation but the disinfectant solutions for their cleaning have been barely explored. The purpose of the present study was to evaluate the antibacterial effects of a Brazilian green propolis glycolic (PGL) solution on the removal of biofilm from the surface of two maxillofacial elastomers (room temperature vulcanization [RTV] and high consistency silicone rubber [HCR]). A total of 42 specimens (3×10 mm) were made with these two elastomers and Staphylococcus aureus was allowed to grow for 24 hours on the surface of the biomaterials. Specimens (n=3) were randomly allocated to the following treatment groups by immersion for 15 minutes: 0.9% saline solution; 2% chlorhexidine gluconate (CHX); 11%, 16% and 20% PGL; and Daro Brand® antimicrobial liquid agent and antibacterial gel soap. Quantitative assessment was performed by counting the number of colony-forming units (CFU). Two-way ANOVA was employed for data analysis. Specimens of both maxillofacial elastomers treated with PGL solutions at concentrations of 11%, 16% and 20%, as well as 2% CHX, did not reveal CFU/mL of the S. aureus strain, indicating the broad spectrum of antibiofilm action of these disinfectant solutions. In addition, the Daro Brand® antimicrobial liquid agent showed lower CFU/mL in both maxillofacial elastomers compared to the Daro Brand® gel soap and 0.9% saline solution. Therefore, disinfection with PGL solutions is effective and might be considered to be a promising alternative in eliminating the S. aureus biofilm from specimens of maxillofacial elastomers by immersion for 15 minutes.


Ariani, N., Visser, A., Teulings, M. R., Dijk, M., Rahardjo, T. B., Vissink, A., & van der Mei, H. C. (2015). Efficacy of cleansing agents in killing microorganisms in mixed species biofilms present on silicone facial prostheses--an in vitro study. Clinical Oral Investigations, 19(9), 2285–2293.

Ariani, N., Vissink, A., van Oort, R. P., Kusdhany, L., Djais, A., Rahardjo, T. B., van der Mei, H. C., & Krom, B. P. (2012). Microbial biofilms on facial prostheses. Biofouling, 28(6), 583–591.

Awale, S., Shrestha, S. P., Tezuka, Y., Ueda, J. Y., Matsushige, K., & Kadota, S. (2005). Neoflavonoids and related constituents from Nepalese propolis and their nitric oxide production inhibitory activity. Journal of Natural Products, 68(6), 858–864.

Capistrano, H. M., de Assis, E. M., Leal, R. M., Alvarez-Leite, M. E., Brener, S., & Bastos, E. M. (2013). Brazilian green propolis compared to miconazole gel in the treatment of Candida-associated denture stomatitis. Evidence-based Complementary and Alternative Medicine, 2013, 947980.

Cavalcante, F. S., Saintive, S., Carvalho Ferreira, D., Rocha Silva, A. B., Guimarães, L. C., Braga, B. S., Dios Abad, E., Ribeiro, M., & Netto Dos Santos, K. R. (2021). Methicillin-resistant Staphylococcus aureus from infected skin lesions present several virulence genes and are associated with the CC30 in Brazilian children with atopic dermatitis. Virulence, 12(1), 260–269.

Chamaria, A., Aras, M. A., Chitre, V., & Rajagopal, P. (2019). Effect of chemical disinfectants on the color stability of maxillofacial silicones: An in vitro study. Journal of Prosthodontics, 28(2), e869–e872.

Azevedo, M. N., Marques, N. T., Fonseca, M., Schuch, L. F., de Arruda, J., Santos, V. R., Mesquita, R. A., & Moreno, A. (2021). Disinfectant effects of Brazilian green propolis alcohol solutions on the Staphylococcus aureus biofilm of maxillofacial prosthesis polymers. The Journal of Prosthetic Dentistry, S0022-3913(21)00193-1.

Caxias, F. P., Dos Santos, D. M., Bannwart, L. C., de Moraes Melo Neto, C. L., & Goiato, M. C. (2019). Classification, history, and future prospects of maxillofacial prosthesis. International Journal of Dentistry, 2019, 8657619.

Souza, R. F., Silva-Lovato, C. H., de Arruda, C. N., Regis, R. R., Zanini, A. P., Longo, D. L., Peracini, A., de Andrade, I. M., Watanabe, E., & Paranhos, H. F. (2019). Efficacy of a propolis solution for cleaning complete dentures. American Journal of Dentistry, 32(6), 306–310.

Santos, D. M., Goiato, M. C., Moreno, A., Pesqueira, A. A., Dekon, S. F. C., & Guiotti, A. M. (2012). Effect of addition of pigments and opacifier on the hardness, absorption, solubility and surface degradation of facial silicone after artificial ageing. Polymer Degradation and Stability, 97(8):1249–1253.

Filié Haddad, M., Coelho Goiato, M., Micheline Dos Santos, D., Moreno, A., Filipe D'almeida, N., & Alves Pesqueira, A. (2011). Color stability of maxillofacial silicone with nanoparticle pigment and opacifier submitted to disinfection and artificial aging. Journal of Biomedical Optics, 16(9), 095004.

Goiato, M. C., Pesqueira, A. A., Ramos da Silva, C., Gennari Filho, H., & Micheline Dos Santos, D. (2009). Patient satisfaction with maxillofacial prosthesis. Literature review. Journal of Plastic, Reconstructive & Aesthetic Surgery, 62(2), 175–180.

Goiato, M. C., Zucolotti, B. C., Mancuso, D. N., dos Santos, D. M., Pellizzer, E. P., & Verri, F. R. (2010). Care and cleaning of maxillofacial prostheses. The Journal of Craniofacial Surgery, 21(4), 1270–1273.

Gonçalves, N. L., Borges, V. M., de Arruda, J., Dos Santos, E. G., Diniz, I., Madeira, M., & Moreno, A. (2020). Antimicrobial effects of photodynamic therapy on Staphylococcus aureus biofilm grown on a specific acrylic resin surface for ocular prostheses. Photodiagnosis and Photodynamic Therapy, 32, 102042.

Guiotti, A. M., Cunha, B. G., Paulini, M. B., Goiato, M. C., Dos Santos, D. M., Duque, C., Caiaffa, K. S., Brandini, D. A., Narciso de Oliveira, D. T., Brizzotti, N. S., & Gottardo de Almeida, M. T. (2016). Antimicrobial activity of conventional and plant-extract disinfectant solutions on microbial biofilms on a maxillofacial polymer surface. The Journal of Prosthetic Dentistry, 116(1), 136–143.

Guiotti, A. M., da Silva, E., Catanoze, I. A., de Carvalho, K., Malavazi, E. M., Goiato, M. C., Dos Santos, D. M., & de Almeida, M. (2018). Microbiological analysis of conjunctival secretion in anophthalmic cavity, contralateral eye and ocular prosthesis of patients with maxillofacial abnormalities. Letters in Applied Microbiology, 66(2), 104–109.

Hatamleh, M. M., Polyzois, G. L., Nuseir, A., Hatamleh, K., & Alnazzawi, A. (2016). Mechanical properties and simulated aging of silicone maxillofacial elastomers: Advancements in the past 45 Years. Journal of Prosthodontics, 25(5), 418–426.

Henriques, M., Sousa, C., Lira, M., Elisabete, M., Oliveira, R., Oliveira, R., & Azeredo, J. (2005). Adhesion of Pseudomonas aeruginosa and Staphylococcus epidermidis to silicone-hydrogel contact lenses. Optometry and Vision Science, 82(6), 446–450.

Jablonski, R. Y., Veale, B. J., Coward, T. J., Keeling, A. J., Bojke, C., Pavitt, S. H., & Nattress, B. R. (2021). Outcome measures in facial prosthesis research: A systematic review. The Journal of Prosthetic Dentistry, 126(6), 805–815.

Kubiliene, L., Laugaliene, V., Pavilonis, A., Maruska, A., Majiene, D., Barcauskaite, K., Kubilius, R., Kasparaviciene, G., & Savickas, A. (2015). Alternative preparation of propolis extracts: comparison of their composition and biological activities. BMC Complementary and Alternative Medicine, 15, 156.

Li, X., Kolltveit, K. M., Tronstad, L., & Olsen, I. (2000). Systemic diseases caused by oral infection. Clinical Microbiology Reviews, 13(4), 547–558.

Miranda, N. B., de Arruda, J., de Almeida, S., Dos Santos, E. G., Medeiros, I. S., & Moreno, A. (2019). Optical parameters and hardness of two maxillofacial elastomers after immersion in different solutions of Brazilian green propolis extract. The Journal of Prosthetic Dentistry, 122(2), 168–175.

Moreno, A., Dos Santos, D. M., Lamartine de Moraes Melo Neto, C., Luiz de Melo Moreno, A., de Magalhães Bertoz, A. P., & Goiato, M. C. (2020). In vitro evaluation of the effect of different disinfectants on the biofilm of Staphylococcus epidermidis and Staphylococcus aureus formed on acrylic ocular prostheses. PloS One, 15(10), e0240116.

Moreno, A., Goiato, M. C., dos Santos, D. M., Haddad, M. F., Pesqueira, A. A., & Bannwart, L. C. (2013). Effect of different disinfectants on the microhardness and roughness of acrylic resins for ocular prosthesis. Gerodontology, 30(1), 32–39.

Mulcare, D. C., & Coward, T. J. (2019). Suitability of a mobile phone colorimeter application for use as an objective aid when matching skin color during the fabrication of a maxillofacial prosthesis. Journal of Prosthodontics, 28(8), 934–943.

Paranhos, R. M., Batalhão, C. H., Semprini, M., Regalo, S. C., Ito, I. Y., & de Mattos, M. (2007). Evaluation of ocular prosthesis biofilm and anophthalmic cavity contamination after use of three cleansing solutions. Journal of Applied Oral Science, 15(1), 33–38.

Pinheiro, J. B., Vomero, M. P., do Nascimento, C., Watanabe, E., Paranhos, H., Coto, N. P., Dias, R. B., Oliveira, V. C., & Silva-Lovato, C. H. (2018). Genomic identification of microbial species adhering to maxillofacial prostheses and susceptibility to different hygiene protocols. Biofouling, 34(1), 15–25.

Rahman, A. M., Jamayet, N. B., Nizami, M., Johari, Y., Husein, A., & Alam, M. K. (2019). Effect of aging and weathering on the physical properties of maxillofacial silicone elastomers: A systematic review and meta-analysis. Journal of Prosthodontics, 28(1), 36–48.

Sforcin J. M. (2016). Biological properties and therapeutic applications of propolis. Phytotherapy Research, 30(6), 894–905.

Shui, Y., Li, J., Lyu, X., & Wang, Y. (2021). Phytotherapy in the management of denture stomatitis: A systematic review and meta-analysis of randomized controlled trials. Phytotherapy Research, 35(8), 4111–4126.

Sonnahalli, N. K., & Chowdhary, R. (2020). Effect of nanoparticles on color stability and mechanical and biological properties of maxillofacial silicone elastomer: A systematic review. Journal of Indian Prosthodontic Society, 20(3), 244–254.

Tong, S. Y., Davis, J. S., Eichenberger, E., Holland, T. L., & Fowler, V. G., Jr (2015). Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clinical Microbiology Reviews, 28(3), 603–661.

Yamaga, M., Tani, H., Nishikawa, M., Fukaya, K., Ikushiro, S. I., & Murota, K. (2021). Pharmacokinetics and metabolism of cinnamic acid derivatives and flavonoids after oral administration of Brazilian green propolis in humans. Food & Function, 12(6), 2520–2530.




How to Cite

SILVA, R. S. O.; ROCHA, F. F. .; ARRUDA, J. A. A. de .; SILVA, I. F. V. da .; BHERING, C. L. B.; SANTOS, V. R. .; MORENO, A. . Effects of Brazilian green propolis glycolic solutions against bacterial biofilm on elastomers used in maxillofacial prostheses. Research, Society and Development, [S. l.], v. 11, n. 3, p. e40111326739, 2022. DOI: 10.33448/rsd-v11i3.26739. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/26739. Acesso em: 15 jun. 2024.



Health Sciences