Antifungal performance of essential oils in breadmaking by in situ, in vitro and active packaging evaluation – a review




Active packaging; Clean label; Molds; Natural antimicrobials; Plant-based metabolites; Preservation technologies.


Due to antifungal properties, the application of essential oils in bread has attracted commercial interest due to its highly perishable. Their limited shelf life is due to their high water activity and is dependent on good manufacturing practices and proper storage and distribution methods. The main spoilage microorganisms in bread are molds. Therefore, the present study aimed to provide an integrative review of scientific literature related to recent studies on the application of essential oils to limit or prevent the development of spoilage fungi in breadmaking. The approach includes the use of essential oils as an additive in the formulation of bread through addition in the dough, as surface protection or in active packaging. Essential oils are composed of bioactive substances with antifungal properties that are suitable for the preservation of bread. High volatility, high costs, and strong odors are characteristics that restrict their applicability. Therefore, it is important to evaluate the dosage, forms of application, different mechanisms of antimicrobial action, and the effects of their individual or combined use with other preservation technologies. In this way, essential oils there may be inhibitory to spoilage microorganisms while maintaining adequate technological and sensorial characteristics in breadmaking.


Adel, A. M., Ibrahim, A. A., El-Shafei, A. M., & Al-Shemy, M. T. (2019). Inclusion complex of clove oil with chitosan/β-cyclodextrin citrate/oxidized nanocellulose biocomposite for active food packaging. Food Packaging and Shelf Life, 20, 100307.

Almada-Érix, C. N., Almada, C. N., Pedrosa, G. T. S., Biachi, J. P., Bonatto, M. S., Schmiele, M., Nabeshima, E. H., Clerici, M. T. P. S., Magnani, M., & Sant'Ana, A. S. (2022). Bread as probiotic carriers: Resistance of Bacillus coagulans GBI-30 6086 spores through processing steps. Food Research International, 155, 111040.

Araújo, R. C. Z. (2009). Embalagens ativas com ervas aromáticas e condimentares na conservação de pães artesanais. 88p. Dissertação (Mestrado em Ciência de Alimentos). Universidade Federal de Lavras.

Assadpour, E., & Jafari, S. (2019). A systematic review on nanoencapsulation of food bioactive ingredients and nutraceutical by various nanocarriers. Critical Reviews in Food Science and Nutrition, 81, 209-219.

Atares, L., & Chiralt, A. (2016). Essential oils as additives in biodegradable films and coatings for active food packaging. Trends in Food Science and Technology, 48, 51-62.

Bagheri, L., Khodaei, N., Salmieri, S., Karboune, S., & Lacroix, M. (2020). Correlation between chemical composition and antimicrobial properties of essential oils against most common food pathogens and spoilers: In-vitro efficacy and predictive modelling. Microbial Pathogenesis, 2020, 104212.

Bakhtiary, F., Sayevand, H. R., Khaneghah, A. M., Haslberger, A. G., & Hosseini, H. (2018). Antibacterial efficacy of essential oils and sodium nitrite in vacuum processed beef fillet. Applied Food Biotechnology, 5,1-10.

Bakkali, F. S., Averbeck, D., & Idaomar, M. (2008). Biological effects of essential oils – A review. Food and Chemical Toxicology, 46, 446-475.

Barba, F. J., Esteve, M. J., & Frígola, A. (2014). Bioactive components from leaf vegetable products. In Atta-ur-Rahman (Ed.), Studies in natural products chemistry (pp. 321-346). Oxford: Elsevier.

Bizzo, H. R, Rezende, C. M., Hovell, A. M. C. (2009). Brazilian essential oils: general view, developments and perspectives. Química Nova, 31, 588-594.

Bora, H., Kamle, M., Mahato, D. K., Tiwari, P., & Kumar, P. (2020). Citrus essential oils (CEOs) and their applications in food: An overview. Plants, 9, 357.

Callaway, T. R., Carroll, J. A., Arthington, J. D., Edrington, T. S., &erson, R. C., and Ricke, S. C. (2011). Citrus products and their use against bacteria: potential health and cost benefits. In R. Watson, J. L. Gerald, & V. R. Preedy (Eds.), Nutrients, dietary supplements, and nutriceuticals: Cost analysis versus clinical benefits (pp. 277-286). New York: Humana Press.

Cardoso, P. G et al. (2007). Diferenciação morfológica e molecular de fungos produtores de pectinases: Penicillium expansum e Penicillium griseoroseum. Brazilian Journal of Microbiology, 38, p. 71-77.

Carmo, E. S., Oliveira, E. L., & Souza, E. L. (2008). The potential of Origanum vulgare (Lamiaceae) essential oil in inhibiting the growth of some food-related Aspergillus species. Brazilian Journal of Microbiology, 39, 362-367.

Cauvain, S. P. (2015). Technology of breadmaking. (3rd ed.). New York: Springer International Publishing.

Císarová, M., Hleba, L., Medo, J., Tančinová, D., Mašková, Z., Čuboň, J., Kováčik, A., Foltinová, D., Božik, M., & Klouček, P. (2020). The in vitro and in situ effect of selected essential oils in vapour phase against bread spoilage toxicogenic aspergilli. Food Control, 110, 107007.

Císarová, M., Hleba, L., Tančinová, D., Florková, M., Foltinová, D., Charousová, I., Vrbová, K., Božik, M., & Klouček, P. (2018). Inhibitory effect of essential oils from some Lamiaceae species on growth of Eurotium spp. isolated from bread. Journal Microbiology Biotechnology Food Science, 8, 857-862.

Clemente, I., Aznar, M., & Nerín, C. (2019). Synergistic properties of mustard and cinnamon essential oils for the inactivation of foodborne moulds in vitro and on Spanish bread. International Journal of Food Microbiology, 298, 44–50.

Dainelli, D., Gontard, N., Spyropoulos, D., Beuken, E. Z., & Tobback, P. (2008). Active and intelligent food packaging: legal aspects and safety concerns. Trends in Food Science and Technology, 19, S103-S112.

De Azeredo, H. M. (2013). Antimicrobial nanostructures in food packaging. Trends in Food Science and Technology, 30, 56-69.

De Corato, U., Maccioni, O., Trupo, M., & Di Sanzo, G. (2010). Use of essential oil of Laurus nobilis obtained by means of a supercritical carbon dioxide technique against postharvest spoilage fungi. Crop Protection, 29, 142-147.

Debonne, E., Bockstaele, F.V., Leyn, I., Devlieghere, F., & Eeckhout, M. (2018). Validation of in-vitro antifungal activity of thyme essential oil on Aspergillus niger and Penicillium paneum through application in par-baked wheat and sourdough bread. LWT - Food Science and Technology, 87, 368-378.

Fernandes, R. P. P., Trindade, M. A., Lorenzo, J. M., & Melo, M. P. (2018). Assessment of the stability of sheep sausages with the addition of different concentrations of Origanum vulgare extract during storage. Meat Science, 137, 244-257.

Fernandez, F., Vodorotz, Y., Courtney, P., Pascall, M. A. (2006). Extended shelf-life of soy bread using modified atmosphere packaging. Journal of Food Protection, 69, 693-698.

Freire, J. M. (2008). Óleos essenciais de canela, manjerona e anis estrelado: caracterização química e atividade biológica sobre S. aureus, E. coli e A. flavus e A. parasiticus. 68 p. Dissertação (Mestrado em Agroquímica) – Universidade Federal de Lavras: Lavras.

Gasperini, A. M. (2014). Efeito de óleos essenciais sobre o crescimento e produção de aflatoxinas por Aspergillus flavus. Dissertação (Mestrado em Ciência de Alimentos) – Universidade Estadual de Campinas: Campinas.

Gholamian, S., Nourani, M., & Bakhshi, N. (2021). Formation and characterization of calcium alginate hydrogel beads filled with cumin seeds essential oil. Food Chemistry, 338, 128143.

Gonçalves, N. D., Pena, F. L., Sartoratto, A., Derlamelina, C. B., Duarte, M. C. T., Antunes, A. E. C., & Prata, A. S. (2017). Encapsulated thyme (Thymus vulgaris) essential oil used as a natural preservative in bakery product. Food Research International, 96, 154-160.

Grande-Tovar, C. D., Chaves-Lopez, C., Serio, A., Rossi, C., & Paparella, A. (2018). Chitosan coatings enriched with essential oils: Effects on fungi involved in fruit decay and mechanisms of action. Trends in Food Science and Technology, 78, 61-71.

Gutiérrez, L., Sánchez, C., Batlle, R., & Nerín, C. (2009). New antimicrobial active package for bakery products. Trends in Food Science and Technology, 20, 92-99.

Gyawali, R., Ibrahim, S. A. (2014). Natural products as antimicrobial agents. Food Control, 46, 412–429.

Hyldgaard, M., Mygind, T., & Meyer, R. L. (2012). Essential oils in food preservation: mode of action, synergies, and interactions with food matrix components. Frontiers in Microbiology, 3, 12.

Jarvis, B. (2001). Mould spoilage of food. Process Biochemistry, 7, 11-14.

Jiang, J., Lan, W., Sameen, D. E., Ahmed, S., Qin, W., Zhang, Q., Chen, H., Dai, J., He, L., & Liu, Y. (2020). Preparation and characterization of grass carp collagen-chitosan-lemon essential oil composite films for application as food packaging. International Journal of Biological Macromolecules, 160, 340-351.

Ju, J., Xie, Y., Yu, H., Guo, Y., Cheng, Y., Qian, H., & Yao, W. (2020). A novel method to prolong bread shelf life: Sachets containing essential oils components. LWT - Food Science and Technology, 131, 109744.

Ju, J., Xu, X., Xie, Y., Guo, Y., Cheng, Y., Qian, H., & Yao, W. (2018). Inhibitory effects of cinnamon and clove essential oils on mold growth on baked foods. Food Chemistry, 240, 850-855.

Khan, S., Hashmi, S., & Saleem, Q. (2013). Microbial spoilage of bakery products and its control by preservatives. Shodhankan, 2, 169-177.

Klich, M. A. (2002). Identification of Common Aspergillus species. Amsterdam: Centraalbureau voor Schimmelautures, 116 p.

Krisch, J., Rentskenhand, T., Horváth, G., Vágvölgyi, C. (2013). Activity of essential oils in vapor phase against bread spoilage fungi. Acta Biologica Szegediensis, 57, 9-12.

Kumar, U., Kumari, P., Sinha, D., Yadav, M., Singh, D., & Singh, B. K. (2020). Antimicrobial activity of essential oils against plant pathogenic fungi: A review. International Journal of Inclusive Development, 6, 37-44.

Legan, J. D., Voysey, P. A. (1991). Yeast spoilage of bakery products and ingredients. Journal of Applied Bacteriology, 70, 361-371.

Liewen, M. B., & Marth, E. H. (1985). Growth and inhibition of microorganisms in the presence of sorbic acid: A Review. Journal Food Protection, 48, 364-375.

Long, N. N. V., Joly, C., & Dantigny, P. (2016). Active packaging with antifungal activities. International Journal of Food Microbiology, 220, 73–90.

Mahmoud, A. E. (1994). Antifungal action and antiaflatoxigenic properties of some essential oil constituents. Letters in Applied Microbiology, 19, 110–113.

Mahmoudzadeh, M., Hosseini, H., Nasrollahzadeh, J., Khaneghah, A. M., Rismanchi, M., Chaves, R. D., Shahraz, F., Azizkhani, M., Mahmoudzadeh, L., & Haslberger, A. G. (2016). Antibacterial activity of Carum copticum essential oil against Escherichia coli O157:H7 in meat: Stx genes expression. Current Microbiology, 73, 265-72.

Maisanaba, S., Llana-Ruiz-Cabello, M., Gutiérrez-Praena, D., Pichardo, S., Puerto, M., Prieto, A. I., Jos, A., & Cameán, A. M. (2016). New advances in active packaging incorporated with essential oils or their main components for food preservation. Journal Food Reviews International, 33, 447-515.

Mendes, K. D. S., Pereira Silveira, R. C. de C., & Galvão, C. M. (2019). Use of the bibliographic reference manager in the selection of primary studies in integrative reviews. Texto e Contexto Enfermagem, 28, 1–13.

Nascimento, G. K. S., Rocha, L. O. F., & Schmiele, M. (2021). Potential of enhancing agents in replacing chemical additives in bread processing. In: D. T. Santos, R. A. Castillo-Torres and G. B. F Carvalho (Eds), The Food Industry: Perceptions, Practices and Future Prospects (pp. 13-43). New York: Nova Science Publisher.

Nazzaro, F. I. D., Fratianni, F., Coppola, R., & De Feo, V. (2017). Essential oils and antifungal activity. Pharmaceuticals, 10, 86.

Nedorostova, L., Kloucek, P., Kokoska, L., & Stolcova, M. (2008). Comparison of antimicrobial properties of essential oils in vapour and liquid phase against foodbourne pathogens. Planta Medica, 74, PI6. http://

Neves, N. A., Gomes, P. T. G., Carmo, E. M. R., Silva, B. S., Amaral, T. N., & Schmiele, M. (2020). Sourdough and jaboticaba (Plinia cauliflora) for improvement on pan bread characteristics. Research, Society and Development, 9, e90691110552.

Neves, N. A., Gomes, P. T. G., Morais, L. A., Silveira, M. P., & Schmiele, M. (2021). Produção e avaliação das características tecnológicas e sensoriais de pães obtidos a partir de fermentação natural e acréscimo de cagaita (Eugenia dysenterica DC.). Revista Brasileira de Agrotecnologia, 11, 39-48.

Neves, N. A., Gomes, P. T. G., & Schmiele, M. (2020). An exploratory study about the preparation and evaluation of sourdough breads with araticum pulp (Annona crassiflora Mart.). Research, Society and Development, 9, e956998036.

Nguefack, J., Leth, V., Amvam, P. H., & Mathur, S. B. (2004). Evaluation of five essential oils from aromatic plants of Cameroon for controlling food spoilage and mycotoxin producing fungi. International Journal of Food Microbiology, 94, 329-334.

Nielsen, P. V., & Rios, R. (2000). Inhibition of fungal growth on bread by volatile components from spices and herbs, and the possible application in active packaging, with special emphasis on mustard essential oil. International Journal of Food Microbiology, 60, 219–229.

Oliveira, M. A., Gonzaga, M. L. C., Bastos, M. R. S., Magalhães, H. C. R., Benevides, S. D., Furtado, R. F., Zambelli, R. A., & Garruti, D. S. (2020). Packaging with cashew gum/gelatin/essential oil for bread: Release potential of the citral. Food Packaging and Shelf Life, 23, 100431.

Otoni, C. G., Pontes, S. F. O., Medeiros, E. A. A., & Soares, N. F. F. (2014). Edible films from methylcellulose and nanoemulsions of clove bud (Syzygium aromaticum) and oregano (Origanum vulgare) essential oils as shelf life extenders for sliced bread. Journal of Agricultural and Food Chemistry, 62, 5214-5219.

Pankaj, S. K., Shi, H., & Keener, K. M. (2018). A review of novel physical and chemical decontamination technologies for aflatoxin in food. Trends in Food Science and Technology, 71, 73-83.

Pereira, M. C., Vilela, G. R., Costa, L. M. A. S., Silva, R. F., Fernandes, A. F., Fonseca, E. W. N., and Picolli, R. H. (2006). Inhibition fungi growth through of utilization essential oils of spice. Ciência e Agrotecnologia, 30, 731-738.

Pinto, E., Gonc, M. J., Hrimpeng, K., Pinto, J., & Vaz, S. (2013). Antifungal activity of the essential oil of Thymus villosus sub sp. lusitanicus against Candida, Cryptococcus, Aspergillus and dermatophyte species. Industrial Crops and Products, 51, 93–99.

Pitt, J. I. & Hocking, A. D. (1997). Fungi and food spoilage. 2. ed. London: Blackie Academic and Professional.

Pontes, S. F. O. (2013). Desenvolvimento de nanoemulsões de óleos essenciais incorporadas em filme de metilcelulose para uso em alimentos. Tese (Doutorado). Universidade Federal de Viçosa.

Preedy, V. R. (2016). Essential Oils in Food Preservation, Flavor and Safety. London, Academic Press.

Rahman, M., Islam, R., Hasan, S., Zzaman, W., Rana, R., Ahmed, S., Roy, M., Sayem, A., Matin, A., Raposo, A., et al. (2022). A comprehensive review on bio-preservation of bread: an approach to adopt wholesome strategies. Foods, 11, 319.


Rehman, A., Jafari, S. M., Aadil, R. M., Assadpour, E., Randhawa, M. A., Mahmood, S. (2020). Development of active food packaging via incorporation of biopolymeric nanocarriers containing essential oils. Trends in Food Science and Technology, 101, 106-121.

Rehman, S., Hussain, S., Nawaz, H., Ahmad, M, M., Murtaza, M, A., & Rizvi, A, J. (2007). Inhibitory effect of citrus peel essential oils on the microbial growth of bread. Pakistan Journal of Nutrition, 6, 558-561.

Reis, J. B., Figueiredo, L. A., Castorani, G. M., Veiga, S. M. O. M. (2020). Evaluation of antimicrobial activity of essential oils against food pathogens. Brazilian Journal of Health Review, 3, 342-363.

Rodriguez, A., Nerin, C., & Battle, R. (2008). New cinnamon-based active paper packaging against Rhizopus stolonifer food spoilage. Journal of Agricultural and Food Chemistry, 56, 6364-6369.

Rosa, C. G., Melo, A. P. Z., Sganzerla, W. G., Machado, M. H., Nunes, M. R., Maciel, M. V. O. B, Bertoldi, F. C., & Barreto, P. L. M. (2020). Application in situ of zein nanocapsules loaded with Origanum vulgare Linneus and Thymus vulgaris as a preservative in bread. Food Hydrocolloids, 99, 105339.

Ruiz, M. C. P., Sanchez, R. A. S., Ramos, S. V., Munoz, J. V. T., & Moorillón, G. V. N. (2012). Antifungal effect of Mexican oregano (Lippia berlandieri Schauer) essential oil on a wheat flour-based medium. Journal of Food Science, 77, 441-445.

Salgado-Nava, A. A., Hernández-Nava, R., López-Malo, A., & Jiménez-Munguía, M. T. (2020). Antimicrobial activity of encapsulated Mexican oregano (Lippia berlandieri Schauer) essential oil applied on bagels. Frontiers in Sustainable Food Systems, 4, 537091.

Santos, F.S., Novales, M. G. (2012). Essential oils from aromatic herbs as antimicrobial agents. Current Opinion in Biotechnology, 23, 136–141.

Santos, J. L. P. Bernardi, A., O., Morassi, L. L. P., Silva, B. S., Copetti, M. V., & Sant'Ana, A. (2016). Incidence, populations and diversity of fungi from raw materials, final products and air of processing environment of multigrain whole meal bread. Food Research International, 87, 103-108.

Santoyo, S. R., Llorıa, L., Jaime, E., Ibanez, F. J., & Senor, G. (2006). Supercritical fluid extraction of antioxidant and antimicrobial compounds from Laurus nobilis: Chemical and functional characterization. European Food Research Technology, 222, 565-571.

Saranraj, P., & Geetha, M. (2012). Microbial spoilage of bakery products and its control by preservatives. International Journal of Pharmaceutical and Biological Archives, 3(1), 38 48.

Seiler, D. (1992). Basic guide to product spoilage and hygiene. Part 1: Mould growth on bread. Chorleywood Digest, 124, CCFRA, Chipping Campden, UK, p.23.

Settanni, L., & Corsetti, A. (2008). Application of bacteriocins in vegetable food biopreservation. International Journal of Food Microbiology, 121, 123-138.

Sharma, N., & Tripathi, A. (2006). Effects of Citrus sinensis (L.) Osbeck epecarp essential oil on growth and morphogenesis of Aspergillus niger (L.) Van Tieghem. Microbiological Research. 163, 337-344.

Sharma, P., Ahuja, A., Dilsad Izrayeel, A. M., Samyn, P., & Rastogi, V. K. (2022). Physicochemical and thermal characterization of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) films incorporating thyme essential oil for active packaging of white bread. Food Control, 133(November 2021).

Sil, A., Pramanik, K., Samantaray, P., Mondal, F. M., & Yadav, V. (2020). Essential oils: A boon towards eco-friendly management of phytopathogenic fungi. Journal of Entomology and Zoology Studies, 8, 1884-1891.

Silva, F. C. (2008). In vitro and in vivo effect of condiment essential oils on fungi that occur in postharvest on strawberry and papaya fruits. Masters dissertation. Lavras: UFLA, 85 p.

Silva, S. J. M., Spencer, P. V. D., Pinto, N. A. V. D., & Schmiele, M. (2020). Antimicrobial performance on molds isolated from muffin and antioxidant capacity of raw and roasted coffee oils (Coffea arabica L.). Research, Society and Development, 9, e9289109285.

Silveira, M. P., Neves, N. A., Silveira, J. V. W., & Schmiele, M. (2019). Nanotechnology applied to cereal grains and cereal-based products and its food safety. In: G. Molina, Inamuddin, F. M. Pelissari, and A. M. Asiri (Eds), Food Applications of Nanotechnology (pp. 211-223). Boca Raton: CRC Press.

Simões, C. M. O., & Spitzer, V. (2007). Óleos voláteis. In: C. M. O. Simões, E. P. Schenkel, G. Gosmann, J. C. P. Mello, L. A. Mentz, & P. R. Petrovick (Eds), Farmacognosia: da planta ao medicamento (pp. 467-496). Porto Alegre/Florianópolis: UFRGS/UFSC.

Siqui, A. C., Sampaio, A. L. F., Sousa, M. C., Henriques, M. G. M. O., & Ramos, M. F. S. (2000). Óleos essenciais - potencial antiinflamatório. Biotecnologia, Ciência e Desenvolvimento, 16, 38-43.

Smid, E. J., & Gorris, L. G. M. (2007). Natural antimicrobials for food preservation, p. 285-308. M. S. Rahman (ed.), Handbook of food preservation (2nd ed.) (pp. 237-258). New York: CRC Press.

Soliman, K. M., & Badeaa, R. I. (2002). Effect of oil extracted from some medicinal plants on different mycotoxigenic fungi. Food and Chemical Toxicology, 40, 1669–1675.

Sun, Q., Li, J., Sun, Y., Chen, Q., Zhang, L., & Le, T. (2020). The antifungal effects of cinnamaldehyde against Aspergillus niger and its application in bread preservation. Food Chemistry, 317, 126405.

Tanackov, D. K.; Dimić, G. R.(2013). Antifungal activity of essential oils in the control of food-borne fungi growth and mycotoxin biosynthesis in food. In: A. Méndez-Vilas (Ed), Microbial pathogens and strategies for combating them: science, technology and education (pp. 838-849). Badajoz: Formatex Research Center.

Tatar, Y., Fadavi, A., & Koohsari, H. (2021). Mitigation of Aspergillus flavus and its aflatoxins in wheat grains by gamma irradiation and calcium oxide. Journal of Crop Protection, 10, 261-270.

Tian, J., Ban, X., Zeng, H., He, J., Chen, Y, & Wang, Y. (2012). The mechanism of antifungal action of essential oil from dill (Anethum graveolens L.) on Aspergillus flavus. PLoS One, 7, e30147.

Valková, V., Ďúranová, H., Galovičová, L., Vukovic, N. L., Vukic, M., & Kačániová, M. (2021). In vitro antimicrobial activity of lavender, mint, and rosemary essential oils and the effect of their vapours on growth of penicillium spp. In a bread model system. Molecules, 26(13).

Xing, Y., Xihong, L., Qinglian, X., Yun, J., & Lu, Y. (2010). Antifungal activities of cinnamon oil against Rhizopus nigricans, Aspergillus flavus and Penicillium expansum in vitro and in vivo fruit test. International Journal of Food Science and Technology, 45, 1837–1842.

Zambonelli, A., Zechini d’Aulerio, A., Bianchi, A., Albasini, A. (1996). Effects of essential oil on phytopathogenic fungi. Journal of Phytopathology, 144, 491–494.




How to Cite

SILVEIRA, M. P.; PELISSARI, F. M.; SCHMIELE, M. Antifungal performance of essential oils in breadmaking by in situ, in vitro and active packaging evaluation – a review. Research, Society and Development, [S. l.], v. 11, n. 6, p. e1011628547, 2022. DOI: 10.33448/rsd-v11i6.28547. Disponível em: Acesso em: 17 jun. 2024.



Review Article