Influence of testosterone hormone therapy on prostate tissue remodeling in healthy adult rats: use of fractal analysis as a diagnostic method




Prostate ; Testosterone; Fractals; Pathology.


Testosterone therapy is often indicated for the treatment of several pathological conditions related to aging, including osteoporosis and metabolic syndrome. However, some questions persist about the safety of the use of the therapy, due to the increased susceptibility of non-prostatic disease. The histopathological analyzes are based on subjective methodologies, being important to the validation of quantitative methods, such as fractal analysis, to detect subtle morphological changes and transform the complexity of the structures into quantitative analytical data. Therefore, the study investigated the effects of testosterone therapy on the prostatic microenvironment remodeling and the validation of an effective method for tissue analysis. Wistar rats with 150 days olde were divided into two experimental groups (n = 5): Group T: received subcutaneous injections of testosterone cypionate (5mg/kg body weight) diluted in corn oil every other day for 4 weeks; Group C: received subcutaneous injections of corn oil as vehicle. The animals were euthanized at 180 days old, in a CO2 chamber, followed by decapitation. After euthanasia, the ventral prostate was collected, dissected and histologically processed to perform the fractal, stereological and epithelial height analysis. There was a reduction in the fractal dimension in group T, accompanied by an increase in epithelial height. Stereological analysis revealed a decrease in the stromal compartment and an increase in the epithelial compartment. Likewise, the results demonstrate that the fractal analysis was effective in assessing alterations related to hormonal supplementation with testosterone, evidencing prostatic morphological alterations observed in the other methods.

Author Biographies

Izabella Tossato Abonizio, Universidade do Oeste Paulista

Faculdadede Ciências da Saúde

Sarah Raquel César Dourado, Universidade do Oeste Paulista

Faculdade de Ciências da Saúde

Thainá Cavalleri Sousa, Universidade do Oeste Paulista

Programa de Pós-Graduação em Ciência Animal

Maria Luiza Silva Ricardo, Universidade do Oeste Paulista

Programa de Pós-Graduação em Ciência Animal

Wilmer Ramírez-Carmona, Universidade Estadual Paulista

Departamento de Odontologia Restauradora

Leonardo de Oliveira Mendes, Universidade do Oeste Paulista

Mestrado em CIências da Saúde

Programa de Pós-Graduação em Ciência Animal


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How to Cite

ABONIZIO, I. T.; DOURADO, S. R. C.; SOUSA, T. C.; RICARDO, M. L. S.; RAMÍREZ-CARMONA, W. .; MENDES, L. de O. . Influence of testosterone hormone therapy on prostate tissue remodeling in healthy adult rats: use of fractal analysis as a diagnostic method. Research, Society and Development, [S. l.], v. 11, n. 7, p. e39811729772, 2022. DOI: 10.33448/rsd-v11i7.29772. Disponível em: Acesso em: 1 mar. 2024.



Health Sciences