Microorganisms from N95 masks were reused for 36h and then decontaminated by heat which we learned from the COVID-19 pandemic
DOI:
https://doi.org/10.33448/rsd-v11i8.30230Keywords:
Masks; COVID-19; Decontamination; High temperature.Abstract
The aim of this study is to analyze the Microorganisms of N95 masks reused for 36 hours and after heat decontamination. Methodology: Experimental study with a sample of 12 N95 masks reused by professionals in the contaminated area of a material and sterilization center for 36 hours, and after decontamination by heat, where two means of decontamination were applied, the thermo-disinfection by moist heat (90ºC) and dry heat (70ºC) in the cabinet dryer, for 1h of exposure, then seeded in chocolate agar culture medium and incubated 48h in a biological incubator at 37ºC. Results: Before decontamination, there was the development of non-pathogenic microorganisms from the resident flora of the mouth, nose, and skin: gran+ cocci, Bacillus gran-Candida Spp, Staphylococcus, and Streptococcus, between 2 to 8 Colony-forming units (CFU). After decontamination, there was no development of microorganisms. As to the macroscopic aspect, those submitted to thermodisinfection suffered visible structural damage, and tears, preventing reuse, while those submitted to dry heat were intact and in reusable condition, as required by the Centers for Disease Control and Prevention (CDC), regarding the sealing test and adjustment to the face. Conclusion: Considering that many routines established during the COVID-19 pandemic will remain, such as the reuse of N95 masks, solutions need to be found for comfortable use while protecting workers. The method, using dry heat, is a viable, fast and simple alternative to decontaminating N95 masks.
References
Assunção, A. A., Simões, M. R. L., Maia, E. G., Alcantara, M. A., & Jardim, R. (2021). COVID-19: estudo de protocolos de proteção individual para profissionais da saúde. Revista Brasileira de Saúde Ocupacional, (46), e32. 10.1590/2317-6369000042120.
Bartholomew, J. W., & Mittwer T. The gram stain. (1952). Bacteriological reviews, 16(1), 1-29. 10.1128/br.16.1.1-29.1952.
Bôtelho, E. X., Melo, R. O. A., Gusmão, N. B., Ximenes, R. M., &Sena, K. X. F. R. (2022). Prevalência e perfil de resistência aos antimicrobianos de Staphylococcus aureus em hospitais do Brasil: uma revisão integrativa da literatura. Research, Society and Development, 11(6), e2711628744. 10.33448/rsd-v11i6.28744
Brasil. (2010) Descrição dos Meios de Cultura Empregados nos Exames Microbiológicos. Agência Nacional de Vigilância Sanitária, Brasília, DF. Recuperado de: https://www.anvisa.gov.br/servicosaude/microbiologia/mod_4_2004.pdf.
Caruhel, JB, Sigaux, N., Crambert, A., Donat, N., Boddaert, G., Haen, P., & Hoffmann, C. (2021). Military gas mask to protect surgeons when performing tracheotomies on patients with COVID-19. BMJ Mil Health, 167 (3), 214.
Celina, M. C., Martinez, E., Omana, M. A., Sanchez, A., Andres, W., Wiemann, D., Tezak, M., & Dargaville, T. R. (2020). Extended use of face masks during the COVID-19 pandemic - Thermal conditioning and spray-on surface disinfection. Polym Degrad Stab, (179), 109251.
Centers for Disease Control and Prevention (2020). Implementing filtering facepiece respirator (FFR) reuse, including reuse after decontamination, when there are known shortages of N95 respirators. Recuperado de: https://asprtracie.hhs.gov/technical-resources/resource/8092/implementing-filtering-facepiece-respirator-ffr-reuse-including-reuse-after-decontamination-when-there-are-known-shortages-of-n95-respirators.
Chen, T.; Yu, W. H.; Izard, J., Baranova, O. V., Lakshaman, A., & Dewhirst, F. E. (2010). The Human Oral Microbiome Database: a web accessible resource for investigating oral microbe taxonomic and genomic information. Database (Oxford), 2010, pp.baq013. 10.1093/database/baq013.
Coelho, W. E., Moraes, M. A., Bonsonato, F., Pedreira, M., Perfeito, J. A., Gabrielloni, M. C., Peterlini, M. A., Santos, M., & Taminato, M. (2021). Eficácia e segurança de método de descontaminação de máscaras N95/PFF2. The Brazilian Journal of Infectious Diseases, 25, 101064. 10.1016/j.bjid.2020.101064
Cruz, L. F., Souza, I. L. A., Souza, L. D., Araújo, M. G. de F., & Granjeiro, P. A. (2021). A importância da microbiota intestinal e seu papel na infecção hospitalar. Research, Society and Development, 10(10), e489101019166. 10.33448/rsd-v10i10.19166
Fresca, A., Acunã, A. F., Moraes, C., Neves, E. A. J., Moriya, G. A. A., Gnatta, J. R., Miranda, L. L., Lourenço, L. L.; Pereira, M. C. O., Takeiti, M. H., Machado, R. F., Bianconi, R., Caragnato, R. C. A.; Batista Neto, S., Glasenapp, V. B., Poveda, V. B., Aguiar Junior, W. (2020) Recomendações relacionadas ao fluxo de atendimento para pacientes com suspeita ou infecção confirmada pelo Covid-19 em procedimentos cirúrgicos ou endoscópicos. SOBEC. Recuperado de: https://bityli.com/krjiV.
Kumar, A., Kasloff, S. B., Cutts, T., Leung, A., Sharma N., Vazquez-Grande, G., Drew, T., Laframboise, D., Orofino, O., Tanelli, & J. Krishnan, J. (2021). Standard hospital blanket warming cabinets can be utilized for complete moist heat SARS-CoV2 inactivation of contaminated N95 masks for re-use. Sci Rep, 11(1), 18316.
Moraes, E. B., Sanchez, M. C. O., Valente, G. S. C., Souza, D. F., & Nassar, P. R. B. (2020). Segurança dos profissionais de saúde em tempos de COVID-19: uma reflexão. Research, Society and Development, 9 (7), e134973832. 10.33448/rsd-v9i7.3832.
Neves, G. B. C., Bezerra, L. C. S., Portugal, W. N., Lundreger, F., Paiva, J. D. S., Paiva, A. C., Santos, J. L. S., & Ferreira e Pereira, E. B. (2021). Proteção respiratória no centro de material e esterilização em tempos de COVID-19. International Journal of Development Research, 1(10), 50994-50997. 10.37118/ijdr.22871.10.2021
Reinhardt, E. L. (2022). Transmissão da COVID-19: um breve reexame das vias de transmissão por gotículas e aerossóis. Revista Brasileira de Saúde Ocupacional, 10.1590/2317-6369000000221.
Resolução RDC nº 15, de 15 de março de 2012 (2012). Dispõe sobre requisitos de boas práticas para o processamento de produtos para saúde e dá outras providências. Diário Oficial da União, Brasília, DF. https://bvsms.saude.gov.br/bvs/saudelegis/anvisa/2012/rdc0015_15_03_2012.html
Rothe, M., Rohm, E., Mitchell, E., Bedrosian, N., Kelly, C., String, G, & Lantagne, D. (2020). A systematic review of mask disinfection and reuse for SARS-CoV-2. medRxiv (Working Paper). 10.1101/2020.11.11.20229880
Rutala WA, Weber DJ (2019). Guideline for Disinfection and Sterilization in Healthcare Facilities. CDC. https://www.cdc.gov/infectioncontrol/pdf/guidelines/disinfection-guidelines-H.pdf.
Severino, A. J. (2018). Metodologia do trabalho científico. Ed. Cortez.
Silva Filho, P. S. P., Costa, R. E. A. R., Santos, M. B. L., Leal, M. C., Vieira, M. J. A, Rodrigues, F. M., Melo, M. J. A., Ferro, J. A., Rodrigues, K. S., Araújo, F. W. C., Silva, A. P. A., Alves, D. R. C. F., Mendes, A. M., Reis, M. R., Leão, C. A., & Raiol, S. R. N. (2020). A importância do uso de equipamentos de proteção individual (EPI) em tempos de covid-19. Pesquisa, Sociedade e Desenvolvimento, 9 (7), e629974610. 10.33448/rsd-v9i7.4610
Silva, K. M. R., Gualberto, M. A., Santos, J. S., Gomes, M., Costa, I. S., Silva, H. J. N., Aquino, S. V., Carvalho, K. M. G., Melo, D. T., Marinho, A. L. de S., Nascimento, A. V. M., Silva, A. M., Formiga, R. S. A., Abreu, F. S., & Almeida, M. R. M. (2021). Sanitary conduct in coping with COVID-19. Research, Society and Development, 10(6), e42210615448. 10.33448/rsd-v10i6.15448
Souza, R. S. M., Migueis, G. S., Oliveira, W. S., Silva, M. S., &Mendes, V. A. (2022). Uso de Equipamentos de Proteção Individual pela equipe de enfermagem no cenário de pandemia. Research, Society and Development, 11 (2), e4011225447. 10.33448/rsd-v11i2.25447.
Vedovato, T. G., Andrade, C. B., Santos, D. L., Bitencourt, S. M., Almdeida, L. P., & Sampaio, J. F. S. (2021). Trabalhadores(as) da saúde e a COVID-19: condições de trabalho à deriva? Revista Brasileira de Saúde Ocupacional, (46), e1. 10.1590/2317-6369000028520.
Xiang, Y., Song, Q., & Gu, W. (2020). Decontamination of surgical face masks and N95 respirators by dry heat pasteurization for one hour at 70°C. Am J Infect Control, 48(8), 880-882. 10.1016/j.ajic.2020.05.026
Zhiqing, L., Yongyun, C., Wexiang, C., Mengning, Y., Yuanquing, M., Zhenan, Z, Haishan, W., Jie, Z., Kerong, D., Huiwu, L., Fengxiang, L., & Zanjing, Z. (2018). Surgical masks as source of bacterial contamination during operative procedures. Journal of Orthopaedic Translation, 14, 57-62. 10.1016/j.jot.2018.06.002.
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Copyright (c) 2022 Giselda Bezerra Correia Neves; Ana Claudia Paiva; Matheus Gabriel da Silva Cavalcanti Viturino ; José Luís Silva dos Santos ; Douglas Henrique da Silva Ferreira ; Anne Célia Alves Vasconcelos da Silva ; Luana Rocha Lopes ; Hilton Silva Pina Junior ; Wanuska Munique Portugal; Miller da Costa Lima Batista e Silva
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