Micro CT assessment of the effect of single and fractionated X radiation doses in rats tibias





X-Ray Microtomography; X-Ray Therapy; Dose Fractionation; Radiation Dosage; Radiation, Ionizing.


The aim of this study was to evaluate the effect of X radiation in single and fractionated doses in tibias of rats by micro-computer tomography (µCT) analysis. The sample was consisted by 20 male rats, divided into 3 groups: Control, Single dose and Fractionated dose. The rats were submitted to a radiation X exposure on lower limbs. The single dose group was exposed to single radiation of 15 gray (Gy), while the fractionated group was submitted to three irradiation sessions of 5 Gy each, totaling 15 Gy. After 24 hours and 25 days, the rats were euthanized; the tibias were removed and scanned using a µCT unit, SkyScan 1174 Compact Micro-CT (Kontich, Bélgica). The parameters total bone area (Tt.Ar) , cortical bone area (Ct.Ar) , total cross-sectional bone area ratio (Ct.Ar / Tt.Ar) e  cortical thickness (Ct.Th)  for cortical bone, e  bone volume ratio (BV / TV), trabecular number (Tb.N), trabecular thickness (Tb.Th) e  trabecular separation (Tb.Sp), for trabecular bone analysis were evaluated. Data were submitted to one-way ANOVA and Tukey’s test (α = 0.05). The µCT evaluation showed significant differences on Tt.Ar and Tb.Sp parameters (p<0,05). It was observed a lower Tt.Ar in the fractionated group compared to control, and higher Tb.Sp in the group receiving a single dose when compared to the control and fractionated groups. It is concluded, in relation to bone microarchitecture, that the radiation X in fractionated doses presents more deleterious effects on the cortical bone and when in singe doses, more damage on trabecular spaces, leading to a higher porosity.

Author Biography

Stefanya Dias de Oliveira, Federal University of Uberlândia

Graduada em Odontologia pela Universidade Federal de Uberlândia (UFU). Cursando especialização em Gestão da Qualidade em Saúde. Possui graduação em Enfermagem pela Universidade Presidente Antônio Carlos (2011).


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How to Cite

OLIVEIRA, S. D. de; REZENDE, L. N. M.; CAIXETA, R. A. V.; GOMES, C. C.; SOARES, P. B. F.; ALMEIDA, S. M. de; BARBOSA, G. L. de R. Micro CT assessment of the effect of single and fractionated X radiation doses in rats tibias. Research, Society and Development, [S. l.], v. 11, n. 7, p. e53911730510, 2022. DOI: 10.33448/rsd-v11i7.30510. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/30510. Acesso em: 7 jul. 2022.



Health Sciences