Probiotic in diets for Nile tilapia (Oreochromis niloticus) fingerlings
DOI:
https://doi.org/10.33448/rsd-v11i9.30987Keywords:
Aquaculture; Microbiota; Intestinal permeability.Abstract
The objective was to evaluate the effects of the probiotics Bacillus subtilis and Bacillus cereus (4x1011 CFU kg−1) (BSC) in diets for Nile tilapia fingerlings. A total of 450 fish (average weight of 2.81 ± 0.06 g) were distributed in a completely randomized design, in 15 aquariums of 210 liters, consisting of five treatments and three replications. The isoproteic and isoenergetic experimental rations were formulated as follows: BSC1: 0% (control); BSC2: 0.6%; BSC3: 0.8%; BSC4: 1.0%; BSC5: 2.0% of probiotics per kg of feed at a concentration of 4x1011 CFU kg−1. The animals were fed the experimental diets for 30 days. At the end of the study, the productive performance was determined, and bromatological and histological analyzes of the fish were carried out. It was concluded that the probiotic did not influence the zootechnical performance of Nile tilapia fingerlings. The use of 0.8% of BSC appeared as a dietary supplementation strategy for Nile tilapia fingerlings, due to the efficacies found in the main results of body centesimal composition and in adaptations in the histomorphometry of the intestinal villi.
References
Adeoye, A., Jaramillo-Torres, A., Fox, S., Merrifield, D., & Davies, S. (2016). Suplementação de dietas formuladas para tilápia (Oreochromis niloticus) com enzimas exógenas selecionadas: desempenho geral e efeitos na histologia intestinal e microbiota. Animal Feed Science and Technology, 215.
Albuquerque, D. M., Marengo, N. G., Mahl, I., Moura, M. C., Rodriguez-Rodriguez, M. D. P., Galo, J. M., & RIBEIRO, R. P. (2015). Bacillus em dietas para alevinos de tilápia do Nilo, variedade GIFT. Journal of Biosciences, Uberlândia, 31(2), 532-540.
Azevedo, R. V., Fosse Filho, J. C., Pereira, S. L., Cardoso, L. D., Júnior, M. V. V., & Andrade, D. R. (2016). Suplementação com prebiótico, probiótico e simbiótico para juvenis de tambaqui a duas densidades de estocagem. Pesquisa Agropecuária Brasileira, 51 (1), 9-16.
Baldisserotto, B., Cyrino, J. E. P., & Urbinati, E. C. (2014). Biologia e fisiologia de peixes neotropicais de água doce. [S.l: s.n.].
Beck, B. R., Kim, D., Jeon, J., Lee, S. M., Kim, H. K., & Kim, O. J. (2015). The effects of feeding with synbiotic (Pediococcus acidilactici and fructooligosaccharide) enriched adult. Fish and Shellfish Immunology, 54, 602-11.
Boscolo, W.R., Feiden, A., Signor, A., Signor, A. A., Bard, J. J., & Ishida, F. A. (2006). Energia digestível para alevinos de tilápia-do-Nilo (Oreochromis niloticus, L.). Revista Brasileira de Zootecnia, 35, 629-633.
Busacker, G.P., Adelman, I.R., & Goolish, E.M. (1990). Growth. Methods for fish biology (Eds. Schreck, C.B. and Moyle, P.B.). Ameriacan Fisheries Society, Bethesda, Maryland, USA- PCWA- L 446.
Brito, J. M., Ferreira, A. H. C., Junior, H. A. S., Oliveira, A. P. A., Santos, C. H. L., & Oliveira, L. T. S. (2019). Desempenho zootécnico de juvenis de tilápia do Nilo (Oreochromis niloticus) alimentados com cepas probióticas e submetidos a desafio sanitário. Ciência Animal Brasileira, 20 (1),1-9.
Carvalho, J. V. D., Lira, A. D. D., Costa, D. S. P., Moreira, E. L. T., Pinto, L. F. B., Abreu, R. D., & Albinati, R. C. B. (2011). Desempenho zootécnico e morfometria intestinal de alevinos de tilápia-do-Nilo alimentados com" Bacillus subtilis" ou mananoligossacarídeo. Revista Brasileira de Saúde e Produção Animal, 12 (1).
Castro, V. S., Xavier, D. T. O., Silva, A. F. C., Fonseca, J. R. S., Boscolo, W. R B., Feiden, A., Signor, A., & Signor, A. A. (2021). Probióticos do gênero Bacillus em dietas para pós-larvas de tilápia do Nilo (Oreochromis niloticus); Research, Society and Development, 10 (7).
Cechim, F., Signor, A., Cividanes, V., Sales, F., Silva, T., Viana, R. & SADO, R. (2012). Prebiótico (Mananoligossacarídeo-MOS) na nutrição de peixes: Efeitos sobre o desempenho de tilápias-do-Nilo Oreochromis niloticus. Boletim De Indústria Animal, 69 (supl.).
Chai, P. C., Song, X. L., Chen, G. F., Xu, H,. & Huang, J. (2016). Dietary supplementation of combined dietary probiotics Lactococcus lactis BFE920 and Lactobacillus plantarum FGL0001 on innate immunity and disease resistance in olive flounder (Paralichthys olivaceus). Fish and Shellfish Immunology. 42, 177-183.
Cruz, P. M., Ibanez, A. L., Hermosillo, O. A. H., & Saad, H. C. R. (2012). Use of probiotics in aquaculture. ISRN Microbiology: 916845.
Diepers, A. C., Krömker, V., Zinke, C.; Wente, N., Pan, L., Paulsen, K., & Paduch, J. H. (2017). In vitro ability of lactic acid bacteria to inhibit mastitis-causing pathogens. Sustainable Chemistry and Pharmacy, 584-92.
DOAN, H. V., Hoseinifar, S. H., Ringø, E., Esteban, M. Á., Dadar, M., Dawood, M. A. O. & Faggio, C. (2019). Host-Associated Probiotics: A Key Factor in Sustainable Aquaculture. Reviews in fisheries science & aquaculture.
Favero, L. M., Facimoto, C. T., Nuez-Ortín, W. G., Isern-Subich, M.-M., Urach, B., & Pereira, U. P. (2019). Uso de aditivo funcional no crescimento de tilápias do Nilo e na redução da mortalidade por franciselose e estreptococose. Panorama da aquicultura. Ed. 170.
Fe Ckaninova, A., Koscova, J., Mudronova, D., Popelka, P., & Toropilova, J. (2017). The use of probiotic bacteria against Aeromonas infections in salmonid aquaculture. Aquaculture. 469:1–8.
Ferreira, A. H. C., Lopes, J. B., Araripe, M. N. B. A., Monteiro, C. A. B., & ANDRADE, F. T. (2018). Avaliação do efeito da adição de probiótico na dieta de alevinos e juvenis de tilápias-do-Nilo (Oreochromis niloticus) criados em esgoto doméstico tratado. Engenharia Sanitária e Ambiental, 23 (4). 665-674.
Ferreira, A. H. C., Brito, J. M., Lopes, J. B., Santana Júnior, H. A., Batista, J. M. M., Silva, B. R., Souza, E. M, & Amorim, I. L. S. (2015). Probiótico na alimentação de pós-larvas de tilápias-do-Nilo submetidas a desafio sanitário; Revista Brasileira de Saúde e Produção Animal, Salvador, 16.
Galagarza, O. A. S. A., Smith, D. J., Drahos, J. D., Eifert, R.C., Williams, D. D. & Kuhn. (2018). Modulation of innate immunity in Nile tilapia (Oreochromis niloticus) by dietary supplementation of Bacillus subtilis endospores. Fish and Shellfish Immunology. 83 171–179.
Giatsis, C., Sipkema, D., Ramiro-Garcia, J., Bacanu, G. M., Abernathy, J., & Verreth, J. (2018). Probiotic legacy effects on gut microbial assembly in tilapia larvae. Scientific Reports; 6: 33965.
Giri, S. S., Sukumaran, V., & Oviya, M. (2013). Potential probiotic Lactobacillus plantarum VSG3 improves the growth, immunity, and disease resistance of tropical freshwater fish, Labeo rohita. Fish and Shellfish Immunology. 30, 1-7.
He. S., Zhang, Y., Xu, L., Yang, Y., Marubashi, T., & Zhou. Z. (2013). Effects of dietary Bacillus subtilis C-3102 on the production, intestinal cytokine expression and autochthonous bacteria of hybrid tilapia Oreochromis niloticus £ Oreochromis aureus. Aquaculture. 412_413:125_130.
Hoseinifar, S. H., Sun, Y., Wang, A., & Zhou, Z. (2018). Probiotics as means of diseases control in aquaculture, A Review of current knowledge and future perspectives. Frontiers in Microbiology, 9: 2429.
Jatobá, A., Moraes, A.V., Steckert, L. D., & Jesus, G. F. A. (2017). Selection of autochtone probiotic for Astyanax bimaculatus. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 69 (6),1645-1652.
Junqueira, L. C., & Carneiro, J. (2008). Histologia básica. 11.ed. Rio de Janeiro: Guanabara Koogan S.A., 524p.
Lima, Acf., Pizauro Jr., Jm., & Macari, M. et al. (2003). Efeito do uso do probiótico sobre o desempenho e atividade de enzimas digestivas de frangos de corte. Revista Brasileira de Zootecnia, 32 (1), 200-207.
Limbu, S. M., Zhou, L., Sun, S. X., Zhang, M. L., & DU, Z. Y. (2018). Chronic exposure to low environmental concentrations and legal aquaculture doses of antibiotics cause systemic adverse effects in Nile tilapia and provoke differential human health risk, Environment International. 115,205–219.
Liu, H., Wang, S., Cai, Y., Guo, X., Cao, Z., & Zhang, Y. (2017). Dietary administration of Bacillus subtilis HAINUP40 enhances growth, digestive enzyme activities, innate immune responses and disease resistance of tilapia, Oreochromis niloticus. Fish and Shellfish Immunology. 60, 326-333.
Liu, H., Zhang, L., Yi, H., Han, X., & Chi, C. (2016). Identification and characterization of plantaricin Q7, a novel plantaricin produced by Lactobacillus plantarum Q7. LWT - Food Science and Technology. 71, 386-390.
Loh, J. Y. (2017). The Role of probiotics and their mechanisms of action: an aquaculture perspective. World Aquaculture, 48 (1), 19-23.
MardoneS, F. O., Paredes, F., Medina, M., Tello, A., Valdivia, V., Ibarra. R., Correa, J., & Gelcich, S. (2018). Identification of research gaps for highly infectious diseases in aquaculture: the case of the endemic Piscirickettsia salmonis in the Chilean salmon farming industry. Aquaculture. 482: 211–220.
Martínez, K., Monroy, D. M. C., Hamdan, P. A., Castro, M. J., & Becerril, C. D. (2017). Probiotics used in Biofloc system for fish and crustacean culture: A review Cienfuegos International Journal of Fisheries and Aquatic Studie
Mello, H., Moraes, J. R. E., Niza, I. G., Moraes, F. R., Ozório, R. O. A., Shimada, M. T., Filho, J. R. E., & Claudiano, G. S. (2013). Efeitos benéficos de probióticos no intestino de juvenis de Tilápia-do-Nilo. Pesquisa Veterinária Brasileira 33 (6) Rio de Janeiro, June.
Merrifield, D. L., Dimitroglou, A., Foey, A., Davies, S. J., Baker, R. T., Bøgwald, J., Castex, M., & Ringø, E. (2010). The current status and future focus of probiotic and prebiotic applications for salmonids. Aquaculture, 302, (1-2). 1-8.
Mohamed, A. S. M., & John, G. M. F. (2008). Effects of probiotics on the survival, growth and challenge infection in Tilapia nilótica (Oreochromis niloticus), Aquaculture Resarch, 39, 647-656.
Moraes, A. V. D., Pereira, M. D. O., Moraes, K. N., Rodrigues, S. J. P., Jesus, G. F. A., & Jatobá, A. (2018). Autochthonous probiotic as growth promoter and immunomodulator for Astyanax bimaculatus cultured in water recirculation system. Aquaculture Research, único, 1-7.
Morinigo, M., Tapia-Paniagua, S., Chabrillon, M., Diaz-Rosales, P., Banda, I., & Lobo-Ma, C. (2010). Balebona. Intestinal Microbiota Diversity of the Flat Fish Solea senegalensis (Kaup, 1858) Following Probiotic Administration. Microbial Ecology. 60(2):310-319.
Nakandakare, I. B., Iwashita, M. K. P., Dias, C. D. C., Tachibana, L., Ranzani-Paiva, M. J. T., & Romagosa, E. (2013). Incorporação de probióticos na dieta para juvenis de tilápias-donilo: parâmetros hematológicos, imunológicos e microbiológicos; Bol. Inst. Pesca, São Paulo, 39(2): 121 – 135.
Nilton, G. M., & Daniele, M. A. (2015). Quantification of intestinal bacteria, operating cost and performance of fingerlings Nile tilapia subjected to probiotics. Latin American Journal of Aquatic Research; 43(2): 367-373.
Okamura, D., Araújo, F.G., Rosa, P.V., Freitas, R.T.F., Murgas, L.D.S., & Cesar, M.P. (2010). Influência da concentração de benzocaína e do comprimento dos peixes na anestesia e na recuperação de tilápia-do-Nilo. Revista Brasileira de Zootecnia, 39, 971-976.
Reid, G. (2016). Probiotics: definition, scope and mechanisms of action. Best Practice & Research Clinical Gastroenterology, 30 (1), 17-25.
Ringo, E., Lovmo, L., Kristiansen, M., Bakken, Y., Salinas, I., Myklebust, R., & Mayhew, T. M. (2010). Lactic acid bacteria vs. pathogens in the gastrointestinal tract of fish: a review. Aquaculture Research, 41(4), 451-467.
Romero, J., Feijoo, C. G., & Navarrete, P. (2012). Antibiotics in aquaculture use, abuse and alternatives. In Carvalho E, editor. Health and environment in aquaculture. Rijeka, Croatia: In-Tech. 159–198.
Schwarz, K. K., Furuya, W. M., & Natali, M. R. M. (2011). Mananoligossacarídeo em dietas para larvas de tilápia. Revista Brasileira de Zootecnia, 40 (12) 2634-2640.
Silva, A. F. C., Fonseca, J. R S., Carvalho, K. V., Vieira, R. A. R., Freitas, K.F.S., Castro, V. S., Signor, A. A., Signor, A., & Feiden, A. (2021). A; Inclusão de aditivos probióticos em dietas para tilápia do Nilo (Oreochromis niloticus) e seus efeitos no desempenho, parâmetros hematológicos, hepáticos e intestinais, Research, Society and Development, 10(8).
Sipaúba-Tavares, L. H., Baccarin, A. E., & Braga, F. M. S. (2006). Limnological parameters and plankton community responses in Nile tilapia pounds under chicken dung and NPK (4-14-8) fertilizers. Acta Limnologica Brasiliensia, 18(3):335-346.
Sun, Y. Z., Yang, H. L., Ma, R. L., Song, K. & Lin, W. Y. (2011). Molecular analysis of autochthonous microbiota along the digestive tract of juvenile grouper Epinephelus coioides following probiotic Bacillus pumilus administration. Journal of Applied Microbiology. 110, 1093–1103.
Verschuere, L., Rombaut, G., Sorgeloos, P., & Verstraete, W. (2000). Probiotic bacteria as biological control agents in aquaculture, Microbiology and Molecular Biology Reviews, 448, (64), 655-671.
Vieira, B. B., & Pereira, E. L. (2016). Potencial dos probióticos para o uso na aquicultura. Revista da Universidade do Vale do Rio Verde, 14(2).
Zorriehzahra, M. J., Delshad, S. T. M. A., Tiwari, R., Karthik, K., Dhama, K., & Lazado, C. C. (2016). Probiotics as beneficial microbes in aquaculture: an update on their multiple modes of action: a review, Veterinary Quarterly, 36(4), 228-24.
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Copyright (c) 2022 Grace Kelly Goudinho Pires; Débora Tatiane Oliveira Xavier; Herivelto Beck de Souza; Valdir Silva de Castro; Janaina Fernanda Rossetto; Fábio Bittencourt; Aldi Feiden; Altevir Signor
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