Bioprospection of filamentous fungi isolated from mangrove sediments of Pernambuco state for petroderivatives biodegradation

Authors

DOI:

https://doi.org/10.33448/rsd-v11i9.31559

Keywords:

Filamentous fungi; Oil spill bioremediation; Selection of biodegrading microorganisms; Environmental recovery.

Abstract

Activities involving the use of petroleum and its derivatives have been a cause of constant concern for the damage caused to ecosystems, due to the high toxicity and difficult degradation of these compounds. Consequently, several decontamination methods have been carried out, highlighting among them bioremediation, an ecologically viable technique that aims at the application of microorganisms and/or their products to remove or degrade petroleum derivatives and minimize the harmful effects in the impacted areas. In this sense, the use of microorganisms isolated from the ecosystem itself constitutes a promising strategy, considering that they already have mechanisms of resistance to these adverse environmental conditions. Thus, the aim of this work was to evaluate the biodegradation potential of petroderivatives of filamentous fungi isolated from mangrove sediments in the State of Pernambuco, contaminated with the spill that occurred in August 2019 on the coast of Northeast Brazil. For this, four fungal isolates belonging to the genera Aspergillus sp., Penicillium sp., Talaromyces sp. and Trichoderma sp. were subjected to acclimatization in Sabouraud Agar medium containing 1-20% of burnt engine oil, and growth was verified at all concentrations after 72 h. Then, the microorganisms acclimated to 20% were selected for the diesel and kerosene biodegradation test, in Bushnell Haas medium containing the redox indicator 2,6-dichlorophenol-indophenol (DCPIP), obtaining results above 75% after 72 h. However, Aspergillus sp. demonstrated the greatest potential, since it reached 88.4% of biodegradation of both petroderivatives. Thus, it is suggested the use of this promising microorganism in bioremediation processes of ecosystems impacted by oil and derivatives.

References

Ahmed, F., & Fakhruddin, A. N. M. (2018). A review on environmental contamination of petroleum hydrocarbons and its biodegradation. International Journal of Environmental Sciences & Natural Resources, 11(3), 1-7.

Alao, M. B., & Adebayo, E. A. (2022). Fungi as veritable tool in bioremediation of polycyclic aromatic hydrocarbons‐polluted wastewater. Journal of Basic Microbiology.

Al‐Dossary, M. A., Abood, S. A., & Al‐Saad, H. T. (2020). Factors affecting polycyclic aromatic hydrocarbon biodegradation by Aspergillus flavus. Remediation Journal, 30(4), 17-25.

Al-Nasrawi, H. (2012). Biodegradation of crude oil by fungi isolated from Gulf of Mexico. J Bioremed Biodegrad, 3(4), 147-52.

Aparna, A.; Srinikethan, G.; Hedge, S. Effect of addition of biosurfactant produced by Pseudomonas ssp. on biodegradation of crude oil. In International Proceedings of Chemical, Biological & Environmental Engineering., Singapore, v. 6, p. 71-75

Araújo, M. E. D., Ramalho, C. W. N., & Melo, P. W. D. (2020). Pescadores artesanais, consumidores e meio ambiente: consequências imediatas do vazamento de petróleo no Estado de Pernambuco, Nordeste do Brasil. Cadernos de Saúde Pública, 36.

Baniasadi, M., & Mousavi, S. M. (2018). A comprehensive review on the bioremediation of oil spills. Microbial action on hydrocarbons, 223-254.

Barnes, N. M., Khodse, V. B., Lotlikar, N. P., Meena, R. M., & Damare, S. R. (2018). Bioremediation potential of hydrocarbon-utilizing fungi from select marine niches of India. 3 Biotech, 8(1), 1-10.

Benguenab, A., & Chibani, A. (2021). Biodegradation of petroleum hydrocarbons by filamentous fungi (Aspergillus ustus and Purpureocillium lilacinum) isolated from used engine oil contaminated soil. Acta Ecologica Sinica, 41(5), 416-423.

Berde, V. B., Bramhachari, P. V., & Berde, C. P. Bioremediation of Petroleum Contaminated Soils. In Bioremediation and Phytoremediation Technologies in Sustainable Soil Management (pp. 231-249). Apple Academic Press. 2022.

Caixeta, P. C. F. (2021). Minhocas da espécie Eisenia fetida como bioindicador de solo contaminado com óleo de motor remediado por bioestimulação. Trabalho de Conclusão de Curso (Graduação em Engenharia Ambiental) - Universidade Federal de Uberlândia, Uberlândia, 43 f.

Cechinel, L. (2019). Isolamento de fungos a partir de solo com uso intenso de agroquímicos, visando a biorremediação (Bachelor's thesis, Universidade Tecnológica Federal do Paraná).

Chen, Q., Bao, B., Li, Y., Liu, M., Zhu, B., Mu, J., & Chen, Z. (2020). Effects of marine oil pollution on microbial diversity in coastal waters and stimulating indigenous microorganism bioremediation with nutrients. Regional Studies in Marine Science, 39, 101395.

Cruz, G. G. D. (2012). Degradação de querosene por consórcio microbiano misto (Dissertação de Mestardo, Universidade Federal de Pernambuco).

Decesaro, A., Rempel, A., Machado, T. S., Cappellaro, Â. C., Machado, B. S., Cechin, I., ... & Colla, L. M. (2021). Bacterial biosurfactant increases ex situ biodiesel bioremediation in clayey soil. Biodegradation, 32(4), 389-401.

dos Santos, R. A., Rodríguez, D. M., Ferreira, I. N. D. S., de Almeida, S. M., Takaki, G. M. D. C., & de Lima, M. A. B. (2021). Novel production of biodispersant by Serratia marcescens UCP 1549 in solid-state fermentation and application for oil spill bioremediation. Environmental Technology, 1-12.

Durval, I. J. B. (2017). Estudo de um biossurfactante produzido por espécies de Bacillus isoladas de água do mar e seu potencial para biorremediação de derramamento de petróleo (Master's thesis, Universidade Federal de Pernambuco).

El Hanafy, A. A., Anwar, Y., Mohamed, S. A., Al-Garni, S. M. S., Sabir, J. S., Zinadah, O. A. A., & Ahmed, M. M. (2015). Isolation and molecular identification of two fungal strains capable of degrading hydrocarbon contaminants on Saudi Arabian environment. International Journal of Bioengineering and Life Sciences, 9(12), 1215-1218.

Fukushima, L. M. (2022). Coral-Sol um invasor resistente: avaliação da toxicidade aguda de contaminantes associados às atividades petrolífera e petroquímica sobre a espécie Tubastraea coccinea. Trabalho de Conclusão de Curso.

Govarthanan, M., Fuzisawa, S., Hosogai, T., & Chang, Y. C. (2017). Biodegradation of aliphatic and aromatic hydrocarbons using the filamentous fungus Penicillium sp. CHY-2 and characterization of its manganese peroxidase activity. RSC advances, 7(34), 20716-20723.

Gunyar, O. A., & Uztan, A. H. (2021). Environmental mycobiotechnology in special reference to fungal bioremediation. In Nanotechnology Applications in Health and Environmental Sciences (pp. 361-383). Springer, Cham.

Hamouda, R. A., Daassi, D., Hassan, H. A., Hussein, M. H., & El-Sheekh, M. M. (2022). Use of live microbes for oil degradation in situ. In Advances in Oil-Water Separation (pp. 297-317). Elsevier.

Kadri, T., Magdouli, S., Rouissi, T., & Brar, S. K. (2018). Ex-situ biodegradation of petroleum hydrocarbons using Alcanivorax borkumensis enzymes. Biochemical Engineering Journal, 132, 279-287.

Micle, V., Sur, I. M., Criste, A., Senila, M., Levei, E., Marinescu, M., ... & Rogozan, G. C. (2018). Lab-scale experimental investigation concerning ex-situ bioremediation of petroleum hydrocarbons-contaminated soils. Soil and Sediment Contamination: An International Journal, 27(8), 692-705.

Mojarad, M., Alemzadeh, A., Ghoreishi, G., & Javaheri, M. (2016). Kerosene biodegradation ability and characterization of bacteria isolated from oil-polluted soil and water. Journal of Environmental Chemical Engineering, 4(4), 4323-4329.

Montero-Rodríguez, D., Andrade, R. F. S., Ribeiro, D. L. R., Lima, R. A., Araujo, H. W. C., & Campos-Takaki, G. M. (2014). Ability of Serratia marcescens UCP/WFCC 1549 for biosurfactant production using industrial wastes and fuels biodegradation In: Industrial, medical and environmental applications of microorganisms: Current status and trends. Madrid, 211-216.

Moustafa, A. M. (2016). Bioremediation of oil spill in Kingdom of Saudi Arabia by using fungi isolated from polluted soils. International Journal of Current Microbiology and Applied Sciences, 5(5), 680-91.

Nkem, B. M., Halimoon, N., Yusoff, F. M., & Johari, W. L. W. (2019). Isolation and Optimization of Diesel-Oil Biodegradation using Cellulosimicrobium cellulans from Tarball. Pertanika Journal of Science & Technology, 27(3).

Olicón-Hernández, D. R., González-López, J., & Aranda, E. (2017). Overview on the biochemical potential of filamentous fungi to degrade pharmaceutical compounds. Frontiers in microbiology, 8, 1792.

Ozyurek, S.B., Avcioglu, N. H., & Seyis Bilkay, I. (2021). Mycoremediation potential of Aspergillus ochraceus NRRL 3174. Archives of microbiology, 203(10), 5937-5950.

Passos, C. T. D., Burkert, J. F. D. M., Kalil, S. J., & Burkert, C. A. V. (2009). Biodegradação de fenol por uma nova linhagem de Aspergillus sp. isolada de um solo contaminado do sul do Brasil. Química Nova, 32, 950-954.

Radwan, S. S., Al-Mailem, D. M., & Kansour, M. K. (2019). Bioaugmentation failed to enhance oil bioremediation in three soil samples from three different continents. Scientific reports, 9(1), 1-11.

Ribeiro, D.L.R. Avaliação do potencial de degradação de diesel e biodiesel em ambientes impactados utilizando Pseudomonas aeruginosa. Dissertação de Mestrado. Recife: Universidade Federal de Pernambuco, 2014. 165 p.

Sahariah, B. P., & Chatterjee, T. (2022). Bioremediation of Mine tailings from Chhattisgarh, India. Geomicrobiology Journal, 1-11.

Sánchez, C. (2020). Fungal potential for the degradation of petroleum-based polymers: An overview of macro-and microplastics biodegradation. Biotechnology advances, 40, 107501.

Saratale, G., Kalme, S., Bhosale, S., & Govindwar, S. (2007). Biodegradation of kerosene by Aspergillus ochraceus NCIM‐1146. Journal of Basic Microbiology, 47(5), 400-405.

Schwarz, A., Adetutu, E. M., Juhasz, A. L., Aburto-Medina, A., Ball, A. S., & Shahsavari, E. (2019). Response of the fungal community to chronic petrogenic contamination in surface and subsurface soils. Geoderma, 338, 206-215.

Sharma, S., Verma, R., & Pandey, L. M. (2019). Crude oil degradation and biosurfactant production abilities of isolated Agrobacterium fabrum SLAJ731. Biocatalysis and Agricultural Biotechnology, 21, 101322.

Ye, J. S., Yin, H., Qiang, J., Peng, H., Qin, H. M., Zhang, N., & He, B. Y. (2011). Biodegradation of anthracene by Aspergillus fumigatus. Journal of hazardous materials, 185(1), 174-181.

Zahed, M. A., Matinvafa, M. A., Azari, A., & Mohajeri, L. (2022). Biosurfactant, a green and effective solution for bioremediation of petroleum hydrocarbons in the aquatic environment. Discover Water, 2(1), 1-20.

Published

04/07/2022

How to Cite

SILVA, P. G. O. da .; MENDONÇA, R. de S. .; COSTA, E. R. C. .; CAMPOS-TAKAKI, G. M. de .; ANDRADE, R. F. da S. .; MONTERO RODRÍGUEZ, D. . Bioprospection of filamentous fungi isolated from mangrove sediments of Pernambuco state for petroderivatives biodegradation. Research, Society and Development, [S. l.], v. 11, n. 9, p. e11311931559, 2022. DOI: 10.33448/rsd-v11i9.31559. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/31559. Acesso em: 5 oct. 2022.

Issue

Section

Agrarian and Biological Sciences