Pro-inflammatory response markers and neutrophil activity index of mice treated with butaphosphan and challenged with bacterial endotoxin




Organic phosphorus; Cellular immunity; Lipopolysaccharide; Neutrophil; IL-6; IL-1β.


Butaphosphan, an organic source of phosphorus, potentiates humoral and cellular immune responses, however, it is still not clear how this occurs. We evaluate the effect of subcutaneous administration of injectable butaphosphan on pro-inflammatory markers and neutrophil activity index of mice challenged with bacterial lipopolysaccharide. Forty-two mice were randomly divided into three groups: Butaphosphan 50 (BUT50, n=14), that received injections of 50 mg/kg of butaphosphan; Butaphosphan 200 (BUT200, n=14), that received injections of 200 mg/kg of butaphosphan and Control (CTL, n=15), that received placebo. Subcutaneous injections were performed twice daily, for eight consecutive days. At the 8th day, half of animals per group were randomly selected to be challenged with bacterial lipopolysaccharide. Two hours after the challenge animals were euthanized, blood and spleen collected to analyze hematological parameters, circulating levels of interleukin 6 (IL-6), 1β (IL-1B) and myeloperoxidase (MPO) tissue activity. Red blood cell count was higher in butaphosphan treated groups. White blood cell count was lower in BUT200/LPS, as consequence of the decreased neutrophil count. Defense cells and IL-6 were elevated in BUT200 group. The use of butaphosphan ameliorate inflammatory markers, both in challenged and not challenged animals.

Author Biography

Marcio Nunes Corrêa, Universidade Federal de Pelotas

Médico Veterinário, com mestrado em Zootecnia e doutorado em Biotecnologia pela Universidade Federal de Pelotas (UFPel). Atualmente é Professor Titular de Clínica Médica de Grandes Animais, no Departamento de Clínicas Veterinária da Faculdade de Veterinária-UFPel trabalhando com Ruminantes, em especial bovinos leiteiros. É bolsista de produtividade em pesquisa do CNPq desenvolvendo atividades direcionadas ao estudo do Metabolismo Animal, com ênfase em Interações entre Nutrição e Reprodução, Doenças Metabólicas e Desenvolvimento de Fármacos e produtos para Nutrição Animal. Orienta nos Programas de Pós-graduação em Veterinária e Biotecnologia e co-orienta no Programa de Zootecnia. Coordena o NUPEEC, Núcleo de Pesquisa, Ensino e Extensão em Pecuária, da UFPel. Foi Coordenador do Programa de Pós-Graduação em Veterinária (mestrado e doutorado), Diretor de Pós-Graduação e também Diretor de Pesquisa da UFPel, junto a Pró-Reitoria de Pesquisa, Pós-Graduação e Inovação. Também Chefe do Departamento de Clínicas Veterinárias da Faculdade de Veterinária. Tem atuação como consultor em gestão de pessoas e de processos produtivos, inovação tecnológica e formação de profissionais. Atualmente desenvolve atividades de mentoria para 5 Startups e de tutoria para 2 empreendimentos, todos vinculados à UFPel.


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How to Cite

MATTEI , P. .; JACOMETO, C. B.; PEREIRA, R. A. .; MONTAGNER, P.; WEILLER , M. A. A.; TEIXEIRA, R. dos S. .; CORRÊA, M. N. Pro-inflammatory response markers and neutrophil activity index of mice treated with butaphosphan and challenged with bacterial endotoxin. Research, Society and Development, [S. l.], v. 11, n. 10, p. e72111032386, 2022. DOI: 10.33448/rsd-v11i10.32386. Disponível em: Acesso em: 4 oct. 2022.



Agrarian and Biological Sciences