Evaluation of antigens by enzyme immunoassay ELISA during experimental infection in goats with Corynebacterium pseudotuberculosis
DOI:
https://doi.org/10.33448/rsd-v11i12.34549Keywords:
Corynebacterium pseudotuberculosis; Immunodiagnosis; Caseous lymphadenitis; Goats.Abstract
Corynebacterium pseudotuberculosis is the etiologic agent of caseous lymphadenitis (CL), a chronic infectious disease that affects sheep and goats. Numerous serological tests have been developed to detect the disease, being the Enzyme Immunoassay (ELISA) one of the most used due to its advantages of sensitivity and specificity. The present study aimed to evaluate the reactivity of a panel of Corynebacterium pseudotuberculosis antigens in goats experimentally infected with non-forming and biofilm-forming strains. Eighteen Canindé goats were used: group 1 - negative control animals, group 2 - goats infected with non-biofilm-forming bacteria and group 3 - goats infected with a biofilm-forming strain. Recombinant antigens CP40 and PLD, membrane fraction antigens (F3) and C. pseudotuberculosis secreted antigens (BHI) were tested. The sensitivity and specificity of the indirect ELISA tests for all antigens were 100%. However, the optical density (OD) values recorded in the BHI ELISA were lower than those found in the other ELISA tests, suggesting that specific antigens may have better sensitivity and specificity in the diagnosis of CL.
References
Alves, F. S. F., Santiago, L. B., & Pinheiro, R. R. (2007). Linfadenite Caseosa: o Estado da Arte. Documentos 74. Embrapa Caprinos.
Barral, T. D., Mariutti, R. B., Arni, R. K., Santos, A. J., Loureiro, D., Sokolonski, A. R., Azevedo, V., Borsuk, S., Meyer, R., & Portela, R. D. (2019). A panel of recombinant proteins for the serodiagnosis of caseous lymphadenitis in goats and sheep. Microbial Biotechnology, 1-11. 10.1111/1751-7915.13454.
Braithwaite, C. E., Smith, E. E., Songer, J. G., & Reine, A. H. (1993). Characterization of detergent-soluble proteins of Corynebacterium pseudotuberculosis. Veterinary Microbiology. 38, 59-70. 10.1016/0378-1135(93)90075-i
Brasil. (2010) Ovinocaprinocultura. Desenvolvimento Regional Sustentável: Série cadernos de propostas para atuação em cadeias produtivas. (Vol. 7). Brasília, DF: Fundação Banco do Brasil.
Carminati, R., Bahia, R., Moura-Costa, L. F., Paule, B. J. A., Vale, V. L., Regis, L., Freire, S. M., Nascimento, I., Schaer, R., & Meyer, R. (2003). Determinação da sensibilidade e da especificidade de um teste de ELISA indireto para o diagnóstico de linfadenite caseosa em caprinos. Revista de Ciências Medicas e Biológicas, 2(1), 88-93. https://doi.org/10.9771/cmbio.v2i1.4256
Dercksen, D. P., Brinkhof, J. M. A., Dekker-Nooren, T., Van Maanen, K., Bode, C. F., Baird, G., & Kamp, E. M. (2000). A comparison of four serological tests for the diagnosis of caseous lymphadenitis in sheep and goats. Veterinary Microbiology, 75, 167-175. 10.1016/s0378-1135(00)00217-0
Droppa-Almeida, D., Vivas, W. L. P., Silva, K. K. O., Rezende, A. F. S., Simionatto, S., Meyer, R., Lima-Verde, I. B., Delagostin, O., Borsuk, S., & Padilha, F. F. (2016). Recombinant CP40 from Corynebacterium pseudotuberculosis confers protection in mice after challenge with a virulent strain. Vaccine, 34, 1091-1096. 10.1016/j.vacina.2015.12.064
Ellis, J. A., Hawk, D. A., Mills, K. W., & Pratt, D. L. (1991). Antigen specificity of antibody responses to Corynebacterium pseudotuberculosis in naturally infected sheep with caseous lymphadenitis. Veterinary Immunology and Immunopathology, 28, 289-301. 10.1016/0165-2427(91)90121-r
Farias, A. P. F., Rocha Filho, J. T. R., Marchioro, S. B., Moreira, L. S., Marques, A. S., Sá, M. C. A., Oliveira, A. A. S., Alcântara, M. E., Mariutti, R. B., Arni, R. K., Trindade, S. C., & Meyer, R. (2020). rSodC is a potential antigen to diagnose Corynebacterium pseudotuberculosis by enzyme‑linked immunoassay. AMB Express, 10(186), 1-8. 10.1186/s13568-020-01125-0
Greiner, M., Sohr, D., & Göbel, P. (1995). A modified ROC analysis for the selection of cut-off values and the definition of intermediate results of serodiagnostic tests. Journal of Immunolgical Methods, 185, 123-132. 10.1016/0022-1759(95)00121-p
Hodgson, A. L. M., Carter, K., Tachedjian, M., Krywult, J., Corner, L. A., McColl, M., & Cameron, A. (1999). Efficacy of an ovine caseous lymphadenitis vaccine formulated using a genetically inactive form of the Corynebacterium pseudotuberculosis phospholipase D. Vaccine, 17, 802-808. 10.1016/s0264-410x(98)00264-3
Kaba, J., Kutschke, L., & Gerlach, G. F. (2001). Development of an ELISA for the diagnosis of Corynebacterium pseudotuberculosis infections in goats. Veterinary Microbiology. 78, 155-163. 10.1016/s0378-1135(00)00284-4
Lipsky, B. A., Goldberger, A. C., Tompkins, L. S., & Plorde, J. J. (1982). Infections caused by nondiphtheria Corynebacteria. Reviews of infectious diseases. 4, 1220-1235. 10.1093/clinídeos/4.6.1220
Menzies, P. I., Hwang, Y. T., & Prescott, J. F. (2004). Comparison of an interferon-gamma to a phospholipase D enzyme-linked immunosorbent assay for diagnosis of Corynebacterium pseudotuberculosis infection in experimentally infected goats. Veterinary Microbiology, 100, 129-137. 10.1016/j.vetmic.2004.01.012
Mina, S., Staerck, C., Marot, A., Godon, C., Calenda, A., Bouchara, J. P., & Fleury, M. J. J. (2017). Scedosporium boydii CatA1 and SODC recombinant proteins, new tools for serodiagnosis of Scedosporium infection of patients with cystic fibrosis. Diagnostic Microbiology and Infectious Disease, 89(4), 282-287. 10.1016/j.diagmicrobio.2017.08.013
Moura-Costa, L. F. (2002). Meio sintético quimicamente definido para o cultivo de Corynebacterium pseudotuberculosis. Revista Brasileira de Saúde e Produção Animal, 3(1), 1-9. https://www.bvs-vet.org.br/vetindex/periodicos/revista-brasileira-de-saude-e-producao-animal/3-(2002)-1/meio-sintetico-quimicamente-definido-para-o-cultivo-de-corynebacterium
Mouckle, C. A., Menzies, P. I., Li, Y., Hwang, T. Y., & Van Wesenbeeck, M. (1992). Analysis of the immunodominant antigens of Corynebacterium pseudotuberculosis. Veterinary Microbiology, 30, 47-58. 10.1016/0378-1135(92)90093-9
Paule, B. J. A., Meyer, R., Moura-Costa, L. F., Bahia, R. C., Carminati, R., Regis, L. F., Vale, V. L. C., Freire, S. M., Nascimento, I., Schaer, R., & Azevedo, V. (2004). Three-phase partitioning as an efficient method for extraction/concentration of immunoreactive excreted–secreted proteins of Corynebacterium pseudotuberculosis, Protein Expression and Purification, 34(2), 311-316. 10.1016/j.pep.2003.12.003
Rebouças, M. F., Loureiro, D., Barral, T. D., Seyffert, N., Raynal, J. T., Sousa, T. J., Figueiredo, H. C. P., Azevedo, V., Meyer, R., & Portela, R. W. (2020). Cell wall glycolipids from Corynebacterium pseudotuberculosis strains with different virulences differ in terms of composition and immune recognition. Brazilian Journal of Microbiology, 51(4), 2101-2110. 10.1007/s42770-020-00343-9
Sá, M. C. A., Veschi, J. L. A., Santos, G. B., Amanso, E. A., Oliveira, S. A. S., Mota, R. A., Veneroni-Gouveia, G., & Costa, M. M. (2012). Activity of disinfectants and biofilm production of Corynebacterium pseudotuberculosis. Pesquisa Veterinária Brasileira, 33(11), 1319-1324. http://www.pvb.com.br/portal/download_artigo/MTMxMnwyMDIyMDkxMDEyNTQ1NQ
Sampaio, P. G., Vale, V. L. C., Moura-Costa, L. F., Fraga, R. E., Santos, H. H. M., Sá, M. C. A., Bastos, B. L., Rocha Filho, J. T. R., Trindade, S. C., & Nascimento, R. J. M. (2019). Padronização de técnicas por citometria de fluxo, para avaliar viabilidade e aspectos da interação entre Corynebacterium pseudotuberculosis e células fagocitárias murinas. PUBVET, 13(11), 1-9. 10.31533/pubvet.v13n11a443.1-9
Seyffert, N., Guimarães, A. S., Pacheco, L. G. C., Portela, R. W., Bastos, B. L., Dorella, F. A., Heinemann, M. B., Lage, A. P., Gouveia, A. M. G., Meyer, R., Miyoshi, A., & Azevedo, V. (2010). High seroprevalence of caseous lymphadenitis in Brazilian goat herds revealed by Corynebacterium pseudotuberculosis secreted proteins-based ELISA. Research in Veterinary Science, 88, 50-55. 10.1016/j.rvsc.2009.07.002
Seyffert, N., Silva, R. F., Jardin, J., Silva, W. M., Castro, T. L. P., Tartaglia, N. R., Santana, K. T. O., Portela, R. W., Silva, A., Miyoshi, A., Le Loir, Y., & Azevedo, V. (2014). Serological proteome analysis of Corynebacterium pseudotuberculosis isolated from different hosts reveals novel candidates for prophylactics to control caseous lymphadenitis. Veterinary Microbiology, 174(1-2), 255-260. 10.1016/j.vetmic.2014.08.024
Simionatto, S., Marchioro, S. B., Galli, V., Brum, C. B., Klein, C. S., Rebelatto, R., Silva, E. F., Borsuk, S., Conceicão, F. R., & Dellagostin, O. A. (2012). Immunological characterization of Mycoplasma hyopneumoniae recombinant proteins. Comparative Immunology, Microbiology and Infectious Diseases, 35, 209-216. 10.1016/j.cimid.2012.01.007
Xu, H., Lohr, J., & Greiner, M. (1997). The selection of ELISA cut-off points for testing antibody to Newcastle disease by two-graph receiver operating characteristic (TG-ROC) analysis. Journal Immunology Methods, 208, 61-64. 10.1016/S0022-1759(97)00128-2
Zerbinati, J., Greve, I. C., Leal, R. F., Amorin, L. M. P. V., Silva, D. L., Viegas, S. R. A. A., Peixoto, A. P. C., Carminati, R., & Cerqueira, R. B. (2007). Produção e padronização de um antígeno para um teste ELISA indireto no diagnóstico da linfadenite caseosa em soros caprinos. Revista Academica Ciência Animal, 5(3), 285-293. 10.7213/cienciaanimal.v5i3.10116
Zwirner, N. W. (1996). Elisa. In: Margni, R. A. Imunologia e Imunoquímica. (5a ed.). Panamericana.
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Copyright (c) 2022 Victoria Christiane Wense Gazarian Calderon; José Tadeu Raynal Rocha Filho; Maria Conceição Aquino de Sá; Bruno Lopes Bastos; Soraya Castro Trindade; Natalia Augusto da Silva Cavalcante; Antonio Pedro Fróes de Farias; Ricardo Wagner Dias Portela ; Ricardo Wagner Dias Portela ; Vasco Azevedo; Roberto Meyer
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