In vitro antagonistic activity of Sclerotium sp. by Bacillus sp.
DOI:
https://doi.org/10.33448/rsd-v11i13.35351Keywords:
Biocontrol; Phytopathogenic fungus; Rhizosphere bacteria.Abstract
Bacteria from the genus Bacillus sp. have a high potential for the biological control of phytopathogenic fungi. Therefore, in the present research we proposed to evaluate the antagonistic capacity of Bacillus sp. isolates against the phytopathogenic fungus Sclerotium sp. The tests were performed using two methods (dual and modified dual), also using five Bacillus sp. isolates (LBM01, LBM02, LBM03, LBM04 and LBM06). Mycelial growth evaluations were performed on BDA culture medium. All experiments were performed in triplicate and the plates were incubated under a 12 h photoperiod at 28±1 ºC for 7 days. Radial growth of the pathogens was measured at the intervals of 24, 48, 72, 96, 120, 144 and 168 h. The percentage of inhibition was calculated using the equation: I% = [(C-T)/C]x100. All Bacillus sp. isolates tested were able to inhibit the mycelial growth of Sclerotium sp. within 72 h, while isolates LBM01, LBM03 and LBM06 were able to maintain inhibition for 168 h. The isolate LBM06 showed the highest percentage of inhibition in all two culture methods used.
References
Cesa-Luna, C., Baez, A., Quintero-Hernández, V., De La Cruz-Enríquez, J., Castañeda-Antonio, M. D., Muñoz-Rojas, J. (2020). The importance of antimicrobial compounds produced by beneficial bacteria on the biocontrol of phytopathogens. Acta Biológica Colombiana, 25(1): 140-154.
Chen, L., Wu, Y. D., Chong, X. Y., Xin, Q. H., Wang, D. X., & Bian, X. (2019). Seed-borne endophytic Bacillus velezensis LHSB1 mediate the biocontrole of peanut stem rot caused by Sclerotium rolfsii. Journal of Applied Microbiology, 128(3): 803-813.
Durairaj, K., Velmurugan, P., Park, J. H., Chang, W. S., Park, Y. J., Senthilkumar, P., Choi, K. M., Lee, J. H., Oh, B. T. (2018). An investigation of biocontrol activity Pseudomonas and Bacillus strains against Panax ginseng root rot fungal phytopathogens. Biological Control, 125: 138-146.
Ferreira de Sá, M. N., Lima, J. S., Nascimento de Jesus, F., Perez, J. O., & Gava, C. A. (2019). Effect of Bacillus sp. and Tricoderma sp. in micelial growth of Sclerotium rolfsii. Acta Brasiliensis, 3(2): 79-81.
Ferreira, M., Lima, J., Nascimento, F., Perezm J., & Gava, C. (2019). Efeito de Bacillus sp. e Trichoderma sp. no crescimento micelial de Sclerotium rolfsii. Acta Brasiliensis, 3(2): 79-81.
Fira, D., Dimkić, I., Berić, T., Lozo, J., & Stanković, S. (2018). Biological control of plant pathogens by Bacillus species. Journal of Biotechnology, 285: 44-55.
Gholami, M., Khakvar, R., & Niknam, G. (2013). Introduction of some new endophytic bacteria from Bacillus and Streptomyces genera as successful biocontrol agents against Sclerotium rolfsii. Archives of Phytopathology and Plant Protection, 47(1): 122-130.
Kumari, P., Bishnoi, S., & Chandra, S. (2021). Assessment of antibiosis potential of Bacillus sp. Against the soil-borne fungal pathogen Sclerotium rolfsii Sacc. (Athelia rolfsiit (Curzi) Tu & Kimbrough). Egyptian Journal of Biological Pest Control, 31(17).
Lopes, U. P., & Michereff, S. J. (2018). Desafios do manejo de doenças radiculares causadas por fungos. (1ª. Ed.): EDUFRPE. 208 p.
Melo, T. A., Nascimento, I. T. V. S., & Serra, I. M. R. de S. (2021). The Bacillus genus applied to the biological control of plant diseases. Research, Society and Development, 10(9):e18110917817.
Montino, S. P. (2021). Uso do Bacillus subtilis como agente de biocontrole de fitopatogenos da cultura de soja. Revista Multidisciplinar de Educacao e Meio Ambiente, 2(3): 14.
Ocegueda-Reyes, M., Casas-Solís, J., Virgen-Calleros, G., Gonzalez-Eguiarte, D., López-Alcocer, E,. & Olalde-Portugal, V. (2020). Aislamiento, identificacion y caracterizacion de rizobacterias antagonicas a Sclerotium cepivorum. Revista Mexicana de Fitopatologia, 38(1): 146-159.
Pereira, A. (2021). Controle biológico de Sclerotium cepivorum mediado por Bacillus spp. na cultura do Alho (Allium sativum). Teses: graduação. Universidade Federal de Santa Catarina, Campus Curitibanos. Curitibanos, Santa Catarina, Brasil.
Rahman, S. F., Singh, E., Pieterse, C. M., & Schenk, P. M. (2018). Emerging microbial biocontrol strategies for plant pathogens. Plant Science, 267: 102-111.
Rangeshwaran, R., & Prasad, R. (2000). Biological control of Sclerotium rot of sunflower. Indian Phytopathology, 53(4): 444-449.
Riungu, G., Muthomi, J., Narla, R., Wagacha, J., & Gathumbi, J. (2008). Management of Fusarium head blight of wheat and deoxynivalenol accumulation using antagonistic micriirganisms. Plant Pathology, 7: 13-19.
Sabaté, D. C., Pérez, C., Petroselli, G., Erra-Balsells, R., & Audisio, M. C. (2018). Biocontrol of Sclerotinia sclerotiorum (Lib.) de Bary on common bean by native lipopeptide-producer Bacillus strains. Microbiological Research, 211: 21-30.
Villarreal-Delgado, M. F., Villa-Rodríguez, E. D., CiraChávez, L. A., Estrada-Alvarado, M. I., Parra-Cota, F. I., & De los Santos-Villalobos, S. 2018. The genus Bacillus as a biological control agent and its implications in the agricultural biosecurity. Revista Mexicana de Fitopatología 36(1): 95-130.
Wu, L., Wu, H-J., Qiao, J., Gao, X., & Borriss, R. (2015) Novel Routes for Improving Biocontrol Activity of Bacillus Based Bioinoculants. Frontiers in Microbiology, 6:1395.
Xu, M., Zhang, X., Yu, J., Guo, Z., Wu, J., Li, X., Chi, Y., & Wan, S. (2020). Biological control of peanut southern blight (Sclerotium rolfsii) by the strain Bacillus pumilus LX11. Biological Science and Technology, 30(5).
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Copyright (c) 2022 Melissa Alezandra Romero Zambrano; Gianny Roger Parra Castanharo; Raimundo Wagner de Souza Aguiar; Aloisio Freitas Chagas Junior
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