Antimicrobial activity of essential oils against pathogenic bacteria of clinical importance
DOI:
https://doi.org/10.33448/rsd-v11i13.35639Keywords:
Essential oils; Antimicrobian activity; Bacterial infections.Abstract
Essential oils are strong antimicrobial candidates with low cost, less toxicity, accessibility, and functionality to circumvent the phenomenon of increasing dissemination of resistant strains of microorganisms. The main objective of the research was to evaluate the sensitivity profile of the bacteria Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus through the use of essential oils. It was an experimental study with a quantitative approach of the antimicrobial action of essential oils against ATCC bacterial strains, obtained commercially. The selection of essential oils was carried out through the recommendations of the Brazilian Pharmacopoeia. The strains were kept in successive repetitions in Tryptic Soy Aga culture medium, until used in the susceptibility tests. The antifungal activity of the essential oils was performed by the technique of diffusion in agar in wells. It was found that the essential oils of C. cassia, M. Alternifolia and E. caryophyllus were able to sensitize all bacterial strains, with emphasis on greater effectiveness for C. cassia against the species S. aureus with an inhibition halo of 32 mm and M. alternifolia on K. pneumoniae with an inhibition halo of 30mm. E. coli and P. aeruginosa bacteria showed resistance to E. globulus. This study intends to contribute to the increase in the knowledge of the antimicrobial susceptibility profile of essential oils applied in the fight against infectious diseases. Further studies are suggested to identify other essential oils and their phytoconstituents with antimicrobial action.
References
Abers, M., Schroeder, S., Goelz, L., Sulser, A., Rose, T. St., Puchalski, K., & Langland, J. (2021). Antimicrobial activity of the volatile substances from essential oils, BMC Complementary Medicine and Therapies, v.21, p. 1-14. 10.1186/s12906-021-03285-3.
Abirami, S., Nishanthini, K., & Mani, P. (2017). Antimicrobial activity and phytochemical screening of the leaf extracts of Eucalyptus globulus. International Journal of Current Pharmaceutical Research, 9(5), 85-89. 10.22159/ijcpr.2017v9i5.22145.
Agência Nacional de Vigilância Sanitária – ANVISA (2019). Farmacopeia Brasileira, v. 1. (6ª. ed.), 903p.
Al Alsheikh, H. M., Sultan, I., Kumar, V., Rather, I. A., Al-Sheikh, H., Jan, A. T., & Hag, Q. M. R. (2020). Plant-Based Phytochemicals as Possible Alternative to Antibiotics in Combating Bacterial Drug Resistance. Antibiotics, 9(8), 1-23. 10.3390/antibiotics9080480.
Allel, K., García, P., Labarca, J., Munita, J. M., Rendic, M., & Undurraga, E. A. (2020). Socioeconomic factors associated with antimicrobial resistance of Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli in Chilean hospitals (2008-2017). Rev. Panam. Salud Publica, v. 44, p. 1-11. 10.26633/RPSP.2020.30.
ALves, E. G., Vinholis, A. H. C., Casemiro, L. A., Furtado, N. A. J. C., Silva, M. L. A., Cunha, W. R., & Martins, C. H. G. (2008). Estudo comparativo de técnicas de triagempara avaliação da atividade antibacteriana de extratos brutos de vegetais espécies e substâncias puras. Química Nova, 31(5), 1224-1229. 10.1590/S0100-40422008000500052.
Alves, M. V. D., Kleibert, K. R. U., Beber, S. C., Plestsh, M U., Staudt, K. J., Alves, I. A., & Colet, C. de F. (2022). Atividade antibacteriana de óleos essenciais frente à Pseudomonas aeruginosa e Staphylococcus aureus. Revista de Biotecnologia & Ciência, 11(1), 32-42. 10.31668/rbc.v11i1.12594.
Amantikir Origem Natural (2022). Óleos essenciais. https://www.amantikir.com.br/categoria-produto/oleos-essenciais/.
Anandhi, M., Tharani, S., Rajeshkumar, S., & Laksm, T. (2022). Antibacterial activity ofCinnamon and clove oil against wound pathogens. J. Popul. Ther. Clin. Pharmacol., 28(2), 41-46. 10.47750/jptcp.2022.871.
Andrade, C. R., Galvão Filho, A. R., Costa, A. C. M., Oliveira, T. A. De, Carneiro, L. C., & Avelino, M. A. G. (2019). Antibacterial and antioxidant effects of Cinnamon and clove essential oils from Madagascar. Materials Today: Proceedings, v. 13, p. 762-770. 10.1016/j.matpr.2019.04.038.
Aslam, B., Wang, W., Arshad, M. I., Khurshid, M., Muzammil, S., Rasool, M. H., Nisar, M. A., Alvi, R. F., Aslam, M. A., Qamar, M. U., Salamat, M. K. F., & Baloch, Z. (2018). Resistência aos antibióticos: um resumo de uma crise global. Infect Drug Resist., v. 11, p. 1645-1658.10.2147/IDR.S173867.
Ayres, M., Ayres Júnior, M., Ayres, D. L., & Santos, A. D. A. (2007). BioEstat: aplicações estatísticas nas áreas das ciências Bio-médicas. Belém: CNPQ, 364p.
Bagheria, L., Khodaeib, N., Salmieria, S., Karbouneb, S., & Lacroixa, M. (2020). Correlation between chemical composition and antimicrobial properties of essential oils against most common food pathogens and spoilers: In-vitro efficacy and predictive modelling. Microbial Pathogenesis, v. 147. 10.1016/j.micpath.2020.104212.
Borotová, P., Galovicová, L., Vukovic, N. L., Vukic, M., Tvrdá, E., & Kacaniová, M. (2022). Chemical and Biological Characterization of Melaleuca alternifoliaEssential Oil, Plants, 11(4), 1-17. 10.3390/plants11040558.
Boukhatem, M. N., Ferhat, M. A., Kameli, A., & Mekarnia, M. (2017). Eucalyptus globulus (Labill.): un arbre à essência aux mille vertus, Phytothérapie, p. 1-12.10.1007/s10298-017-1114-3.
Buehrle, D. J., Shields, R. K., Clarke, L. G., Potoski, B. A., Clancy, C. J., & Bguyen, M. H. (2017). Carbapenem Resistant Pseudomonas aeruginosa Bacteremia: Risk Factorsfor Mortality and Microbiologic Treatment Failure. Antimicrobial Agents and Chemotherapy, 61(1), 1-7. 10.1128/AAC.01243-16.
Carvalho, J. J. V. de, Silva, Boaventura, F. G., A. de C. R. da, Ximenes, R. L., Rodrigues, L. K. C., Nunes, D. A. de A., & Souza, V. K. G. (2021). Bactérias multirresistentes eseus impactos na saúde pública: Uma responsabilidade social, Research, Society and Development, 10(6), 1-11. 10.33448/rsd-v10i6.16303.
Chernov, V. M., Chernova, O. A., Mouzykantov, A. A., Lopukhov, L. L., & Aminov, R. I. (2019). Omics of antimicrobials and antimicrobial resistance, Expert Opinion on Drug Discovery, 14(5), 455-468, 2019. 10.1080/17460441.2019.158888.
Chouhan, S., Sharma, K., & Guleria, S. (2017). Antimicrobial Activity of Some Essential Oils – Present Status and Future Perspectives. Medicines, 4(3), 1-21.10.3390/medicines4030058.
Clinical Laboratory Standards Institute - CLSI. (2019). Performance standards for antimicrobial susceptibility testing: twenty-first informational supplement. NCCLS document M100-521, 34(1), 2019.
Contruccia, B. A., Silva, R., Junior, R. A., & Andreani, D. I. K. (2019). Efeito de Óleos Essenciais Sobre Bactérias Gram-Negativas Isoladas de Alimentos. Ensaios e Ciênc., 23(3), 180-184. 10.17921/1415-6938.2019v23n3p180-184.
Cordeiro, L., Figueiredo, P., Souza, H., Sousa, A., Andrade-Júnior, F., Medeiros, D., Nóbrega, J., Silva, D., Martins, E., Barbosa-Filho, J., & Lima, E. (2020). Terpinen-4-ol as an Antibacterial and Antibiofilm Agent against Staphylococcus aureus, Int. J. Mol. Sci., 21(12), 1-14. 10.3390/ijms21124531.
Correa, L. T., Nicoletti, M. A., De Amorim, C. S., Da Costa, A. R., Leoni, L. A. B., Munõz, J. W. P., & Fukushima, A. R. (2020). Atividade antimicrobiana do óleo essencial de Melaleuca e sua incorporação em um creme mucocutâneo. Fitos, 14(1), 26-37. 10.32712/2446-4775.2020.818.
Da Cruz, L. F., Souza, I. L. A., De Souza, L. D., Araújo, M. G. De F., & Granjeiro, P. A. (2021). The importance of intestinal microbiota and its role in the nosocomial infection. Research, Society and Development, 10(10), e489101019166. 10.33448/rsd-v10i10.19166.
Da Mota, F. S., De Oliveira, H. A., & Souto, R. C. F. (2018). Perfil e prevalência de resistênciaaos antimicrobianos de bactérias Gram-negativas isoladas de pacientes de uma unidade de terapia intensiva. RBAC, 50(3), 270-277.10.21877/2448-3877.201800740.
Dadgostar, P. (2019). Antimicrobial Resistance: Implications and Costs. Infect Drug Resist.,v.12, p. 3903-3910.10.2147/IDR.S234610.
De Assis, K. M., Rêgo, R. L. de A., De Melo, D. F. de, Da Silva, L. M. da, Oshiro-Júnior, J. A., Formiga, F. R., Pires, V. C., De Lima, A. A. N., Converti, A., & Damasceno, B. P. G. de L. (2020). Therapeutic Potential of Melaleuca alternifolia Essential Oilin New Drug Delivery Systems. Curr. Pharm. Des., 26(33), 4048-4055.10.2174/1381612826666200305124041.
De Carvalho, J. J. V., Boaventura, F. G., Da Silva, A. de C. R., Ximenes, R. L., Rodrigues, L. K. C., Nunes, D. A. de A., & De Souza, V. K. G. (2021). Bactérias multirresistentes e seus impactos na saúde pública: Uma responsabilidade social. Research, Society and Development, 10(6), e58810616303. 10.33448/rsd- v10i6.16303.
De Miranda, D. M., & Rocha. (2022). Avaliação da atividade antibacteriana do extrato etanólico de folhas e cascas de Eucalyptus globulus Labill. frente à Escherichia coli e Staphylococcusaureus. Brazilian Journal of Health and Pharmacy, 4(1), 27-38. https://bjhp.crfmg.org.br/crfmg/article/view/161/105.
De Oliveira, T. R., Teixeira, A. L., Barbosa, J. P., De Feiria, S. N. B., Boni, G.C., Maia, F., Anibal, P. C., Wijesinghe, Despotovic, A., Milosevic, B., Milosevic, I., Mitrovic, N., Cirkovic, A., Jovanovic, S., & Stevanovic, G. (2020). Hospital- acquired infections in the adult intensive care unit – Epidemiology, antimicrobial resistance patterns, and risk factors for acquisition and mortality. American Journal of Infection Control, v.48, p.1211-1215. 10.1016/j.ajic.2020.01.009.
Dong, Y., Li, C., Kim, K., Cui, L., & Liu, X. (2021). Genome annotation of disease-causingmicroorganisms, J. Brief. Bioinform., 22(2), 845-854. 10.1093/bib/bbab004.
El Amarani, S., El Ouali Lalami, A., Ez Zoubi, Y., El Moukhafi, K., Bouslmati, R., & Lairini, S. (2021). Evaluation of Identificação de bactérias causadoras de infecçãohospitalar usando fenotipagem clássica. Brazilian Journal of Development, 7(6), 54446-54463. 10.34117/bjdv7n6-036.
Farahmandfar, R., Kenari, R. E., Asnaashari, M., Shahrampour, D., & Bakhshandeh, T. (2019). Bioactive compounds, antioxidant and antimicrobial activities of Arummaculatum leaves extracts as affected by various solvents and extraction methods. Food Sci. Nutr., 7(2), 465-475. 10.1002/fsn3.815.
Frias, F. R., Quer, J., Tabemero, D., Cortese, M. F., Garcia, S. G., Segura, A. R., & Pumarola, T. (2021). Microorganisms as Shapers of Human Civilization, from Pandemics toEven Our Genomes: Villains or Friends? A Historical Approach. Microorganisms, 9(2), 1-54. 10.3390/microrganisms9122518.
Furtado, D. M. F., Silveira, V. S. Da, Carneiro, I. C. Do R. S., Furtado, D. M. F., & Kilishe, M. P. (2019). Consumo de antimicrobianos e o impacto na resistência bacteriana em um hospital público do estado do Pará, Brasil, de 2012 a 2016, Rev Pan Amaz Saude, v. 10, p. 1-8. 10.5123/s2176-6223201900041.
G. K., Hoflin, J. F. (2020). Melaleuca spp essential oil and its medical applicability. A brief review. BJNS, 3(1), 249-258. 10.31415/bjns.v3i1.89.
Gioppo, A., Zancanaro,V., & Bellaver, E. H. (2019). Atividade antibacteriana do óleo essencial de Melaleuca alternifolia frente a isolados multirresistentes produtores de ESBL eKPC causadores de infecções hospitalares, Rev. Biotemas, 32(3), 35-42. 10.5007/2175-7925.2019v32n3p35.
Gonçalves, A. L., Filho, A.A., & Menezes, H. (2005). Estudo comparativo da atividade antimicrobiana de extratos de algumas árvores nativas. Arq. Inst. Biol. 72(3), 353-358.10.1590/1808-1657v72p3532005.
Grove, D. C., & Randall, W. A. (1955) Assay methods of antibiotics: A laboratory manual. (2ª. ed.): Medical Encyclopedia Inc, 238p.
Heimesaat, M. M., Escher, U., Grunau, A., Kühl, A. A., & Bereswill. (2019). Multidrug Resistant Pseudomonas aeruginosa Accelerate Intestinal, Extra-Intestinal, and Systemic Inflammatory Responses in Human Microbiota-Associated Mice With Subacute Ileitis. Front. Immunol., v.10, p.1-15. 10.3389/fimmu.2019.00049.
Horcajada, J. P., Montero, M., Oliver, A., Sorlí, L., Luque, S., Zorilla, S. G., Benito, N., & Grau, S. (2019). Epidemiology and Treatment of Multidrug Resistant and Extensively Drug-Resistant Pseudomonas aeruginosa Infections. Clinical Microbiology Reviews, 32(4), 1-52. 10.1128/CMR.00031-19.
Kačániová, M., Galovičová, L., Valková, V., Tvrdá, E., Terentjeva, M., Žiarovská, J., Kunová, S., Savitskaya, T., Grinshpan, D., Štefániková, J., Felsöciová, S., Vukovic, N., & Kowalczewski, P. L. (2021). Antimicrobial and antioxidant activities of Cinnamomum cassia essential oil and its application in food preservation. Open Chemistry, v.19, p.214-227. 10.1515/chem-2021-0191.
Kharori, N., Stevaux, C., & Ripley, K. (2020). Antibiotics: From the Beginning to the Future: Part 2, Indian J. Pediatr., 87(1), 43-47. 10.1007/s12098-019-03113-0.
Lakatos, E. M., & Marconi, M. de A. (2021). Metodologia científica. (9ª. ed.): Atlas.
Lima, C. V. R., Das Neves, F. J. M., Maior, L. P. S., Pires, L. L. S., Rocha, T. J. M., & Dos Santos, A. F. (2019). Ação antibacteriana do oléo essencial das folhas de Eucalyptus globulus,frente a patógenos multirresitentes. Diversitas Journal, 4(1), 242-252.10.17648/diversitas-journal-v4i1.633.
Liu, Q., Meng, X., Li, Y., Zhao, C. N., Tang, G. Y., & Li, H. B. (2017). Antibacterial and Antifungal Activities of Spices. Int. J. Mol. Sci.,18(6), 1-62.10.3390/ijms18061283.
Majumder, M. D. A. A., Rahman, S., Cohall, D., Bharatha, A., Singh, K., Haque, M., & Hilaire, M. G. S T. (2020). Antimicrobial Stewardship: Fighting Antimicrobial Resistance and Protecting Global Public Health. Infect Drug Resist, v.13, p. 4713-4738. 10.2147/IDR.S290835.
Marchese, A., Barbieri, R., Coppo, E., Orlan, I. E., Daglia, M., Nabavi, S. F., Izadi, M., Abdollahi, M., Nabavi, S, M., & Ajami, M. (2017). Antimicrobial activity of eugenol and essential oils containing eugenol: A mechanistic viewpoint. Crit. Rev. Microbiol., 43(6), 1-22. 10.1080/1040841X.2017.1295225.
Mcewen, S. A., & Collignon, P. J. (2018). Antimicrobial Resistance: a One Health Perspective. Microbiol. Spectr., 6(2), 1-26. 10.1128/microbiolspec.ARBA-0009-2017.
Mervyn, P., Savins, V., Dowell, A., Morrow, S., Allen, G., & Southwell. (2017). Ecotype Variation of Methyl Eugenol Content in Tea Tree (Melaleuca alternifolia and Melaleuca linariifolia). Chem Biodivers, 14(11), 1-15. 10.1002/cbdv.201700278.
Monteiro, N. F., Lima, H. M. R., Da Silva, F. L., Sousa, F. Das C. A., Da Silva, W. C., Reis, L. C. de M., Pimentel, A. de C. C., Furtado, D. R., Rodrigues, A. C. E., Rodrigues, R. P. de S., Alves, A. M. M., Rodrigues, J. P. P., & Monteiro, A. L. (2021). Atividade do óleo essencial de Eucalyptus Globulus no controle de bactérias da cavidade oral, Research, Society and Development, 10(14), 1-12. 10.33448/rsd- v10i14.20091.
Monteiro, R. F. dos S., Santos, V. R. R. dos, Ferreira, A. A. C. T., & Abreu, J. R. G. de. (2020). O uso indiscriminado de antimicrobianos para o desenvolvimento de micro-organismos resistentes, Revista Eletrônica Acervo Saúde, v. sup, 53, p. 1-10. 10.25248/reas.e3597.2020.
Nakamura, A., Kawahara, A., Takahashi, H., Kudam T., & Kimura, B. (2022). Comparison between the Antimicrobial Activity of Essential Oils and Their Components inthe Vapor Phase against Food-related Bacteria. Journal of Oleo Science, 71(3), 411-417. 10.5650/jos.ess21337.
Oulkheir, S., Aghrouch, M., El Mourabit, F., Dalha, F., Graich, H., Amouch, F., Ouzaid, K., Moukale, A., & Chadli, S. (2017). Antibacterial Activity ofEssential Oils Extracts from Cinnamon, Thyme, Clove and Geranium Against a Gram Negative and Gram Positive Pathogenic Bacteria. Journal of Diseases and Medicinal Plants, v. 3, p. 1-5. 10.11648/j.jdmp.s.2017030201.11.
Penteado, A. L. (2021). Atividade do óleo essencial de Cinnamomum cassia contra Staphylococcus aureus - Revisão. Rev. Higiene Alimentar, 35(293).10.37585/HA2021.02atividade.
Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da Pesquisa Científica. (1ª. ed.) Santa Maria: UFSM, NTE, 119f.
Pombo, J. C. P., Ribeiro, E. R., Pinto, R. De L., & Da Silva, B. J. M. (2018). E feito antimicrobiano e sinergístico de óleos essenciais sobre bactérias contaminantes de alimentos, Rev. Segur. Aliment. Nutr., 25(2), 108-117. 10.20396/san.v25i2.8651785.
Puvača, N., Milenković, J., Coghill, T. G., Bursić, V., Petrović, A., Tanasković, N., Pelić, M., Pelić, D. L., & Miljković, T. (2021). Antimicrobial Activity ofSelected Essential Oils against Selected Pathogenic Bacteria: In Vitro Study, Antibiotics, 10(5), 1-14. 10.3390/antibiotics10050546.
Romero, A. C., Carvajal, M. X. Q., & Ruiz, Y. (2018). Stability and antimicrobial activity of eucalyptus essential oil emulsions. Food Science and Technology International, 25(1), 24-37. 10.1177/1082013218794841.
Rook, G., Backhed, F., Levin, B. R., Mcfall Ngai, M. J., & Mclean, A. R. (2017). Evolution, human-microbe interactions, and life history plasticity. Lancet, 390(10093), 521-530. 10.1016/S0140-6736(17)30566-4.
Sampaio, P. da S., Sancho, L. G., & Lago, R. F. do. (2018). Implementação da nova regulamentação para prescrição e dispensação de antimicrobianos: possibilidades e desafios. Cad. Saúde Colet, 26(1). 1590/1414-462X201800010185.
Schneider, E. M., Fujii, R. A. X., & Corazza, M. J. (2017). Pesquisas quali-quantitativas: contribuições para a pesquisa em ensino de ciências. Rev. Pesquisa Qualitativa, 5(9), 569-584. ISSN:2525-8222.
Silva, J. O. da, & Paixão, J. A. da. (2021) Resistência bacteriana e a atuação do farmacêutico na promoção do uso racional de antibacterianos em âmbito hospitalar. Rev. Artigos.com, p. 1-7. ISSN: 2596-0253.
Silva, R. T., Lopes, J. B, A., Oliveira, K. L. L. De., Junior Ribeiro, J. C., & Beloti, V. (2019). Perfil de sensibilidade a antimicrobianos de Bactérias patogênicas humanas isoladas de leite cru. Rev. Inst. Lact. Cândido Tostes., 74(3), 185- 194.10.14295/2238-6416.v74i3.743
Simpkin, V. L., Renwick, M. J., Kelly, R., & Mossialos, E. (2017). Incentivising innovation in antibiotic drug discovery and development: progress, challenges and next steps. J. Antibiot., 70(12), 1087-1096. 10.1038/ja.2017.124.
Solarte, A. L., Astorga, R. J., De Aguiar, F. C., De Frutos, C., Domínguez, B. B., & Huerta, B. (2018). Susceptibility Distribution to Essential Oils of Salmonella enterica Strains Involved in Animal and Public Health and Comparison of the Typhimurium andEnteritidis serotypes, J. Med. Food., 21(9), 946-950.10.1089/jmf.2017.0103.
Spézia, F. P., Siebert, D., Tenfen, A., Cordova, C. M. M. De, Alberton, M. D., & Guedes, A. (2020). Avaliação da atividade antibacteriana de plantas medicinais de uso popular:Alternanthera brasiliana (penicilina), Plantago major (tansagem), Arctostaphylos uva-ursi (uva-ursi) e Phyllanthus niruri (quebra-pedra). Rev. Pan Amaz. Saúde, v. 11. 10.5123/S2176-6223202000127.
Strich, J. R., & Palmore, T. N. (2017). Preventing Transmission of Multidrug-Resistant Pathogensin the Intensive Care Unit. Infect Dis Clin North Am., 31(3), p.535-550. 10.1016/j.idc.2017.05.010.
Suliman, A. H., Ahmed, K. E., Mohamed, B. E., & Babiker, E. E. (2019). Potential of Cinnamon (Cinnamomum Cassia) as an Anti-Oxidative and Anti-Microbial Agent in Sudanese Yoghurt (Zabadi). J. of Dairy and Vet Sci J., 12(20, 1-8. 10.19080/JDVS.2019.12.555833.
Ugarte, M. V., Llorente, C. G., Diaz, J. P., & Gil, A. (2019). Antimicrobial, Antioxidant, andImmunomodulatory Properties of Essential Oils: A Systematic Review. Nutrients, 11(11), 1-29. 10.3390/nu11112786.
Vasconcelos, N. G., Silva, K. E., Croda, J., & Simionatto, S. (2020). Atividade antibacteriana do óleo essencial de Cinnamomum cassia L. em uma cepa de Klebsiella aerogenes resistente a carbapenem e polimixina. Rev. da Soc. Bras. de Med. Trop., v. 53, p.1-4. 10.1590/0037-8682-0032-2020.
Vincent, J. L., Sakr, Y., Singer, M., Loeches, I. M., Machado, F. R., Marshall, J. C., Finfer, S., Pelosi, P., Brazzi, L., Aditianingsih, D., Timsit, J. F., Du, B., Wittebole, X., Máca, J., Kannan, S., Delsol, L. A. G., De Waele, J. J., Mehta, Y., Bonten, M. J. M., Khanna, A. K., Kollef, M., Human, M., & Angus, D. C. (2020). Prevalence and Outcomes of Infection Among Patients in Intensive CareUnits in 2017. JAMA, 323(15), 1478-1487. 10.1001/jama.2020.2717.
Waglechner, N., Culp., E. J., & Wright, G. D. (2021). Ancient Antibiotics, Ancient Resistance. J. EcoSal Plus, 9(2), 2-10. 10.1128/ecosalplus.ESP-0027-2020.
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Copyright (c) 2022 Ana Florise Morais Oliveira; Francisco Laurindo da Silva; Francilene Maria Morais; Reginara Teixeira da Silva; Rodolfo Ritchelle Lima dos Santos; Laudimir Leonardo Walbert Veloso da Silva; Jonathas Morais Oliveira; Cleide Coelho Morais; Kellyane Karen Ferreira Aguiar Cesar
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