Molluscicidal activity against Biomphalaria glabrata of the bioactive nanoemulsion of the essential oil of Thymus vulgaris Linn (Thyme)
DOI:
https://doi.org/10.33448/rsd-v11i15.36784Keywords:
Molluscicidal; Nanoemulsion; Essential oil; Schistosomiasis.Abstract
This study evaluated the molluscicidal activity against the schistosomiasis-transmitting snail (Biomphalaria glabrata) of the nanoemulsion of Thymus vulgaris L. essential oil. The plant material was collected in the municipality of São Luís (MA). The essential oil was extracted by hydrodistillation at 100 °C for 3h, with chemical characterization performed by Gas Chromatography coupled to Mass Spectrometry (GC-MS). The nanoemulsions were prepared using the phase inversion method. For molluscicidal activity, the methodology recommended by the Who was performed, being the LC50 of the essential oil nanoemulsion for their action against the snail obtained by the Probit method. The major constituents found in Thymus vulgaris L. essential oil were carvacrol (27.35%) and thymol (24.12%). The molluscicidal activity of the essential oil nanoemulsion presented an LC50 of 15.04 mg L-1. The results indicate that the essential oil nanoemulsion evaluated is composed of substances that provide and encourage its application, due to its high potential for molluscicidal activity against Biomphalaria glabrata.
References
Araujo, T. D. S., da Costa, J. M. A. R., Ribeiro, F. D. O. S., de Jesus Oliveira, A. C., do Nascimento Dias, J., de Araujo, A. R., ... & de Souza, B. W. S. (2021). Nanoemulsion of cashew gum and clove essential oil (Ocimum gratissimum Linn) potentiating antioxidant and antimicrobial activity. International Journal of Biological Macromolecules, 193, 100-108.
Borges, A. M., Pereira, J., Cardoso, M. G., Alves, J. A., & Lucena, E. M. P. (2012). Determinação de óleos essenciais de alfavaca (Ocimum gratissimum L.), orégano (Origanum vulgare L.) e tomilho (Thymus vulgaris L.). Revista Brasileira de Plantas Medicinais, 14, 656-665.
Bruxel, F., Laux, M., Wild, L. B., Fraga, M., Koester, L. S., & Teixeira, H. F. (2012). Nanoemulsões como sistemas de liberação parenteral de fármacos. Química Nova, 35, 1827-1840.
Cantanhede, S. P. D., Marques, A. D. M., Silva-Souza, N., & Valverde, A. L. (2010). Atividade moluscicida de plantas: uma alternativa profilática. Revista Brasileira de Farmacognosia, 20, 282-288.
Coêlho, M. D. G., da Silva, V. A. R., Pereira, J. R., Akisue, G., da Silva Coêlho, F. A., & Furtado, F. N. (2013). Avaliação “in vitro" do potencial acaricida do óleo essencial de Tagetes minuta frente a Riphicephalus (Boophilus) microplus (Canestrini, 1887). Revista Biociências, 19(1).
Colley, D. G., Bustinduy, A. L., Secor, W. E., & King, C. H. (2014). Human schistosomiasis. The Lancet, 383(9936), 2253-2264.
Costa, K. P., Fonseca, E. S., Andrade, R. E. S., Ferreira, G. S., Rodrigues, L. I. T., da Fonseca, F. S. A., & Martins, E. R. (2021). Atividade antioxidante dos extratos etanólicos e dos óleos essenciais de Xylopia aromática e Piper nigrum. Brazilian Journal of Development, 7(3), 27904-27912.
Finney, D. J. (1952). Probit analysis: a statistical treatment of the sigmoid response curve. Cambridge university press, Cambridge.
Furlani, R., Sousa, M., Rocha, G. N. D. S. A., Vilar, F. C. R., Ramalho, R. C., & Peixoto, R. D. M. (2021). Atividade antibacteriana de óleos essenciais frente aos patógenos de importância na mastite caprina e ovina. Revista Caatinga, 34(3), 702-708.
Galovičová, L., Borotová, P., Valková, V., Vukovic, N. L., Vukic, M., Štefániková, J., ... & Kačániová, M. (2021). Thymus vulgaris essential oil and its biological activity. Plants, 10(9), 1959.
Garcia, C. R., Malik, M. H., Biswas, S., Tam, V. H., Rumbaugh, K. P., Li, W., & Liu, X. (2022). Nanoemulsion delivery systems for enhanced efficacy of antimicrobials and essential oils. Biomaterials Science, 10(3), 633-653.
Khalil, N., Fekry, M., Bishr, M., El-Zalabani, S., & Salama, O. (2018). Foliar spraying of salicylic acid induced accumulation of phenolics, increased radical scavenging activity and modified the composition of the essential oil of water stressed Thymus vulgaris L. Plant Physiology and Biochemistry, 123, 65-74.
Kubitschek-KM, A. R. J., & Zero, J. M. (2014). Development of jojoba oil (Simmondsia chinensis (Link) CK Schneid.) based nanoemulsions. Lat. Am. J. Pharm, 33(3), 459-63.
Lima, T. C. P., de Almeida, A. F., de Oliveira, E. C. P., Carrera Silva Júnior, J. O., Ribeiro Costa, R. M., Pena Matos, A., & Fonseca Gomes, M. R. (2020). Desenvolvimento de nanogel de copaifera reticulata sobre a lesão muscular em ratos usando fonoforese. Saúde e Pesquisa, 13(1).
McClements, D. J., Das, A. K., Dhar, P., Nanda, P. K., & Chatterjee, N. (2021). Nanoemulsion-based technologies for delivering natural plant-based antimicrobials in foods. Frontiers in Sustainable Food Systems, 5, 643208.
Melo, A. F. M., de Sousa, L. D. F. L., do Nascimento Júnior, W., do Nascimento, W. L., da Costa Tenório, R., da Silva Rodrigues, R. R., ... & Santana, L. S. O. S. (2021). Alecrim (rosmarinus officinalis l.) Atividade anti-inflamatória: uma revisão de literatura. Revista de Casos e Consultoria, 12(1), e24346-e24346.
Nunes, C. R., Valente, P. M., da Silva, F. D., & Valente, V. M. M. (2021). Composição química e atividade antifúngica do óleo essencial de Thymus Vulgaris sobre Aspergillus Niger, Penicillium Expansum, Sclerotinia Sclerotiorum E Sclerotium Rolfsii. Brazilian Journal of Development, 7(2), 14250-14260.
Patil, S. M., Ramu, R., Shirahatti, P. S., Shivamallu, C., & Amachawadi, R. G. (2021). A systematic review on ethnopharmacology, phytochemistry and pharmacological aspects of Thymus vulgaris Linn. Heliyon, 7(5), e07054.
Porto, M., Valadares, Y., Silva, C., Santos, R., & Gualberto, S. (2021). Avaliação do potencial antioxidante do óleo essencial de Thymus vulgaris L. (Lamiaceae). Enciclopédia Biosfera, 18(38).
Ribeiro, S. M., Bonilla, O. H., & Lucena, E. M. P. (2018). Influência da sazonalidade e do ciclo circadiano no rendimento e composição química dos óleos essenciais de Croton spp. da Caatinga. Iheringia, Série Botânica., 73(1), 31-38.
Rocha, B. C. A. D. (2013). Extração e caracterização do óleo essencial de tomilho (Thymus vulgaris) (Tese de mestrado, Universidade Federal Rural do Rio de Janeiro).
Rodrigues, E. D. C., Ferreira, A. M., Vilhena, J. C., Almeida, F. B., Cruz, R. A., Florentino, A. C., ... & Fernandes, C. P. (2014). Development of a larvicidal nanoemulsion with Copaiba (Copaifera duckei) oleoresin. Revista Brasileira de Farmacognosia, 24, 699-705.
Salama, M. M., Taher, E. E., & El-Bahy, M. M. (2012). Atividades moluscicida e mosquitocida de óleos essenciais de Thymus capitatus Hoff. et Link. e de Marrubium vulgare L. Revista do Instituto de Medicina Tropical de São Paulo, 54(5), 281-286.
Santos Carvalho, O., Coelho, P. M. Z., & Lenzi, H. L. (Eds.). (2008). Schistosoma mansoni & Esquistossomose: uma visão multidisciplinar. SciELO-Editora FIOCRUZ.
Santos, A. B. D. S., Everton, G. O., Júnior, R. G. D. O. C., Rosa, P. V. S., Pereira, A. P. M., dos Santos Souza, L., ... & Mouchrek Filho, V. E. (2020). Óleos essenciais de Cinnamomum zeylanicum Blume e Plectranthus amboinicus (lour.) Spreng como agentes larvicidas frente as larvas do Aedes aegypti. Brazilian Journal of Development, 6(4), 22355-22369.
Shafiq, S., Shakeel, F., Talegaonkar, S., Ahmad, F. J., Khar, R. K., & Ali, M. (2007). Development and bioavailability assessment of ramipril nanoemulsion formulation. European journal of pharmaceutics and biopharmaceutics, 66(2), 227-243.
Silva, A. G. D. S., França Junior, D. B., Santos, H. F. D. A. C., Moura, H. L. S., Garcia, P. T., Lima, C. S. D. C., ... & Moreira, J. C. R. (2014). Doenças transmissíveis: doenças negligenciadas associadas à pobreza e a vigilância em saúde.
Sugumar, S., Clarke, S. K., Nirmala, M. J., Tyagi, B. K., Mukherjee, A., & Chandrasekaran, N. (2014). Nanoemulsion of eucalyptus oil and its larvicidal activity against Culex quinquefasciatus. Bulletin of entomological research, 104(3), 393-402.
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Copyright (c) 2022 Brendha Araújo de Sousa; Ana Patrícia Matos Pereira; Paulo Victor Serra Rosa; Maria Gizelda Gomes Lages; Francisco Nascimento Silva; Larissa Karla Barros de Alencar; Maria Almira Bulcao Loureiro; Maxwell Cabral Ferreira; Thayson Rodrigues Lopes; Geison Luiz Costa de Castro; Ari Pereira de Araújo Neto; Gustavo Oliveira Everton
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