Streptomyces hygroscopicus oil extract with activities against Aedes aegypti
DOI:
https://doi.org/10.33448/rsd-v12i3.40490Keywords:
Bioinsecticide; Gas chromatography; Secondary metabolites.Abstract
The arboviruses transmitted by the female Aedes aegypti are considered a public health problem and the vector control is essential to reduce the spread of disease-causing pathogens. The main objective of this research was to investigate the chemical composition of the Streptomyces hygroscopicus biomass extract and its larvicidal capabilities against the Aedes aegypti mosquito. Regarding the result of the prospection of the chemical composition of the extract, based on the qualitative colorimetric methodology of the presence of secondary metabolites, there was a positive indication for the alkaloid test and a negative one for flavonoids. Through Gas Chromatography coupled to mass spectrometry it was possible to infer the presence of 9-12-octadecadienoic acid followed by the presence of 9-12-octadecadienoic acid and linoleic acid. The larvicidal activity against Ae. aegypti (LC50) of the extract diluted with 1% DMSO was 155.4 ppm, the highest mortality was with 250ppm reaching 100 porcent. Finally, it was seen that the oily extract of S. hygroscopicus biomass was able to act on Ae. aegypti, showing as a potential larvicidal for this species.
References
Achee, N. Grieco, J. P., Vatandoost, H. Seixas, G., Pinto, J. Ching-NG, L., Martins. & Vontas, J. (2019). Alternative strategies for mosquito-borne arbovirus control. PLOS Neglected Tropical Diseases, 13(1), 1-22. doi: https://doi.org/10.1371/journal.pntd.0006822
Amelia-Yap, Z, H., Azman, A, S., AbuBakar, S., & Low, V. L. Streptomyces derivatives as an insecticide: Current perspectives, challenges and future research needs for mosquito control, Acta Tropica, 229, 2022. https://doi.org/10.1016/j.actatropica.2022.106381.
Bergamo, P., Luongo, D., Miyamoto, J., Cocca, E.; Kishino, S., Ogawa, J., Tanabe, S., & Rossi, M. (2014). Immunomodulatory activity of a gut microbial metabolite of dietary linoleicvacid, 10-hydroxy-cis-12-octadecenoicvacid, associated with improved antioxidant/detoxify. Journal of Functional Foods, 11, 192-202. https://doi.org/10.1016/j.jff.2014.10.007
Bilyk, O., & Luzhetskyy, A. (2016) Metabolic engineering of natural product biosynthesis in actinobacteria. Curr Opin Biotechnol, 42(1), 98-107. 10.1016/j.copbio.2016.03.008.
Brasil - Ministério da Saúde. (2016). Dengue: diagnóstico e manejo clínico: adulto e criança. Brasil: Ministério da Saúde, Secretaria de Vigilância em Saúde, Departamento de Vigilância das Doenças Transmissíveis.
Consoli, R. A. G. B., & Oliveira, R. L. (1994). Principais mosquitos de importância sanitária no Brasil (Vol. 1). Fiocruz.
Desbois, A. P., & Smith, V. J. (2010). Antibacterial free fatty acids: activities, mechanisms of action and biotechnological potential. Applied Microbiology and Biotechnology, 85(6), 1629–1642. 10.1007/s00253-009-2355-3
Forattini, O. P. (2002). Culicidologia Médica (Vol 1). Universidade de São Paulo.
Farouil, L., Duchaudé, Y., Zozo, L., Sylvestre, M., Lafay, F., Marote, P., & Cebrian-Torrejon, G. Cyclic voltammetry of immobilized particles as an alternative pesticide screening method for Aedes aegypti mosquitoes. J Solid State Electrochem, 2023. doi: https://doi.org/10.1007/s10008-023-05398-w
Ganesan, P., Andand, S., Sivanandhan, S., David, R. H. A., Paulraj, M. G., Al-Dhabi, N. A., & Ignacimuthu, S. Larvicidal, ovicidal and repellent activities of Streptomyces enissocaesilis (S12–17) isolated from Western Ghats of Tamil Nadu India. J. Entomol. Zool. Stud, 6(2), 1828–1835, 2018.
Hwang, K. S., Kim, H. U., Charusanti, P., Palsson, B., & Lee, S. Y. (2014). Systems biology and biotechnology of Streptomyces species for the production of secondary metabolites. Biotechnology Advances, 32(2), 255–268. https://doi.org/10.1016/j.biotechadv.2013.10.008
Jaivel, N., Uvarani, C., Rajesh, R., Velmurugan, D., & Marimuthu, P. (2014). Natural occurrence of organofluorine and other constituents from Streptomyces sp. TC1. J Nat Prod, 77(1), 2-8. https://doi.org/10.1021/np400360h
Janaki, T. (2016). Larvicidal activity of Streptomyces cacaoi subsp. cacaoi-M20 against Aedes aegypti. International Journal of Botany Studies, 1(2), 47-49.
Kishimoto, S., Sato, M., Tsunematsu, Y., & Watanabe, K. (2016). Evaluation of Biosynthetic Pathway and Engineered Biosynthesis of Alkaloids. Molecules, 21(8), 1078. https://doi.org/10.3390/molecules21081078
Lopez, S. B. G., Ribeiro, V. G., Rodriguez., J. V., Dorand, F. A. P. S., Salles, T. A; Guimarães, T. S., Alvarenga, E. S. L., Melo, A. C. A., Almeida, R. V., & Moreira, M. F. RNAi-based bioinsecticide for Aedes mosquito control. Sci. Rep, 9, 1-13, 2019. https://doi.org/10.1038/s41598-019-39666-5
Masi, M., Cala, A. Tabanca, N., Cimmino, A., Grenn, I. R., Bloomquist, J. R., Macias, F. A., & Evidente, A. (2016). Alkaloids with Activity against the Zika Virus Vector Aedes aegypti (L.)- Crinsarnine and Sarniensinol, Two New Crinine and Mesembrine Type Alkaloids Isolated from the South African Plant Nerine sarniensis. Nature proceding, 21(11). 10.3390/molecules21111432
Melo, A. R., Garcia, I. J., Serrão, J. E., Santos, H. L., Lima, L. A. R. S., & Alves, S. (2018). N.Toxicity of different fatty acids and methyl esters on Culex quinquefasciatus larvae. Ecotoxicology and Environmental Safety, 154(1), 1-5. 10.1016/j.ecoenv.2018.02.009.
Naine, J., & Devi, S. C. (2014). Larvicidal and Repellent Properties of Streptomyces sp. VITJS4 Crude Extract against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus (Diptera: Culicidae). Polish jornal of microbiology, 63(3), 341-348. 10.22073/pjm-2014-045
Rottig, A., Atasayar, E. J. P., Sproer, C. Schumann, P., Schauer, J., & Steinbuchel, A. (2017). Streptomyces jeddahensis sp. nov., an oleaginous bacterium isolated from desert soil. International Journal of Systematic and Evolutionary Microbiology, 67(6), 1676-1682. 10.1099/ijsem.0.001839
Samy, R. P., & Chow, V. T. K. (2011) Antimicrobial and Phytochemical Analysis of Centella asiatica (L.) R. Nature proceding, 1. hdl:10101/npre.2011.6033
Sayed, A. M., Hassan, M. H. A., Alhadrami, H. A., Hassan, H. M., Goodfellow, M., & Rateb, M. E. Extreme environments: microbiology leading to specialized metabolites. J. Appl. Microbiol, 128(3), 630-657, 2019. https://doi.org/10.1111/jam.14386
Silva, R. M. F., Ribeiro, J. F. A., Freitas, M. C. C., Arruda, M. S. P., Nascimento, M. N., Barbosa, W. L. R., & Rolim Neto, P. J. (2013). Caracterização físico-química e análises por espectrofotometria e cromatografia de Peperomia pellucida L. (H. B. K.). Rev. Bras. Pl. Med, 15(4), 717-726. https://doi.org/10.1590/S1516-05722013000500012
Sivakala, K. K., Gutiérrez-García, K., Jose, P. A., Thinesh, T., Anandham, R., Barona-Gómez, F., & Sivakumar, N. Desert environments facilitate unique evolution of biosynthetic potential in Streptomyces. Molecules, 26(3), 588, 2021. https://doi.org/10.3390/molecules26030588.
Tan, L. T., Ser, H. L. Yin, W. F., Chan, K. G. Lee, L. H., & Goh, B. H. (2015). Investigation of Antioxidative and Anticancer Potentials of Streptomyces sp. MUM256 Isolated from Malaysia Mangrove Soil. Front. Microbiol, 6, 1316. https://doi.org/10.3389
Wagner, H. (1996). Plant Drug Analysis: A Thin Layer Chromatography (2a ed.). Springer.
World Health Organization – WHO. (2005). Guidelines for laboratory and field testing of mosquito larvicides (pp. 1-41).
WHO. (2012). Handbook for integrated vector management. Geneva: World Health Organization.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Jackelly Felipe de Oliveira; Hyago Luiz Rique; Fabíola da Cruz Nunes; Leonor Alves de Oliveira da Silva
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.