Comparison between triglycerides and cholesterol concentrations in saliva and blood

Authors

DOI:

https://doi.org/10.33448/rsd-v9i7.4361

Keywords:

Stomatology; Diagnosis; Triglycerides; Cholesterol; Saliva

Abstract

Introduction: sialochemical studies demonstrate that saliva has biomolecular proportions similar to blood, and can function as a means of diagnosing cardiovascular diseases whose factors are dyslipidemia. Objective: To assess the correlation between blood lipid concentrations and saliva. Methodology: The unstimulated saliva of 40 dentistry students, ages 18 to 29, both genders, was collected by the modified Navazesh method, then centrifuged. A venipuncture for blood collection was performed, then the blood was centrifuged and the serum separated. Labtest® kits were measured for salivary and blood cholesterol and triglycerides using the colorimetric enzymatic method. Statistically, paired Student's t-test and Pearson's correlation test were used. The results were expressed as mean more or less the standard error of the mean (e.p.m). Graph Pad Prism software version 6.01 was used. Results: 72.5% of the participants were female and 27.5% male, the average age was 21.55 ± 0.41 for the female gender and 21.64 ± 1.07 for the male. The average age of individuals of both genders was 21.63 ± 0.41 years. Sialometric data showed an average salivary flow of 0.71 ± 0.15 ml / min. The values of cholesterol and salivary triglycerides were significantly lower when compared to serum values and there was no correlation between these parameters. Conclusion: Saliva showed lower cholesterol and triglyceride concentrations than blood, with no significant correlation of these lipids between fluids.

Author Biographies

José Henrique de Araújo Cruz, Universidade Federal de Campina Grande

Cirurgião-Dentista pela Universidade Federal de Campina Grande, UFCG, Centro de Saúde e Tecnologia Rural, CSTR, Patos/Paraíba, Brasil. Pós-graduando em Ortodontia pelas Faculdades Unidas do Norte de Minas, FUNORTE, Campina Grande/Paraíba, Brasil.

Ingridy Cristiny de Souza Moreira, Universidade Federal de Campina Grande

Cirurgiã-Dentista pela Universidade Federal de Campina Grande, UFCG, Centro de Saúde e Tecnologia Rural, CSTR, Patos/Paraíba, Brasil.

Maria de Fátima Vieira Alves, Universidade Federal de Campina Grande

Cirurgiã-Dentista pela Universidade Federal de Campina Grande, UFCG, Centro de Saúde e Tecnologia Rural, CSTR, Patos/Paraíba, Brasil.

Marcos Andrei da Silva Alves Satyro, Universidade Federal da Paraíba

Farmacêtico pela Universidade Federal da Paraíba, UFPB, João Pessoa/Paraíba, Brasil.

Aline de Farias Diniz, Universidade Federal de Campina Grande

Bióloga pela Universidade Federal da Paraíba, UFPB, João Pessoa/Paraíba, Brasil.

Raline Mendonça dos Anjos, Universidade Federal de Campina Grande

Professora Doutora na Universidade Federal de Campina Grande, UFCG, Centro de Saúde e Tecnologia Rural, CSTR, Patos/Paraíba, Brasil.

Gymenna Maria Tenório Guênes, Universidade Federal de Campina Grande

Professora Doutora na Universidade Federal de Campina Grande, UFCG, Centro de Saúde e Tecnologia Rural, CSTR, Patos/Paraíba, Brasil.

Elizandra Silva da Penha, Universidade Federal de Campina Grande

Professora Doutora na Universidade Federal de Campina Grande, UFCG, Centro de Saúde e Tecnologia Rural, CSTR, Patos/Paraíba, Brasil.

Camila Helena Machado da Costa Figueiredo, Universidade Federal de Campina Grande

Professora Doutora na Universidade Federal de Campina Grande, UFCG, Centro de Saúde e Tecnologia Rural, CSTR, Patos/Paraíba, Brasil.

Abrahão Alves de Oliveira Filho, Universidade Federal de Campina Grande

Professor Doutor na Universidade Federal de Campina Grande, UFCG, Centro de Saúde e Tecnologia Rural, CSTR, Patos/Paraíba, Brasil.

Maria Angélica Satyro Gomes Alves, Universidade Federal de Campina Grande

Professora Doutora na Universidade Federal de Campina Grande, UFCG, Centro de Saúde e Tecnologia Rural, CSTR, Patos/Paraíba, Brasil.

References

Al Rawi, NH. (2010). Salivary lipid peroxidation and lipid profile levels in patients with recent ischemic stroke. J Int Dent Med Res; 3, 57-64.

Al Rawi, NH. (2011). Oxidative stress, antioxidant status and lipid profile in the saliva of type 2 diabetics. Diab Vasc Dis Res. 8, 22-8.

Brasil. (2008). Ministério da Saúde (2009).Secretaria de Vigilância em Saúde. Departamento de Análise de Situação de Saúde. Saúde Brasil 2008: 20 anos de Sistema Único de Saúde (SUS) no Brasil. Brasília: Ministério da Saúde, 416p.

Battino, M, Ferreiro, MS, Gallardo, I, Newman, HN & Bullon, P. (2002). The antioxidant capacity of saliva. Journal of clinical periodontology. 29(3), 189–194.

Curi, R.; Pompeia, C.;Myasaka, C.K.;Procopio, J. (2002)Entendendo as gorduras. São Paulo: 1. Ed, 580p.

Chiappin, S.; Antonelli, G.; Gatti, R.; De Palo, E.F.; (2007). Saliva specimen: A new laboratory tool for diagnostic and basic investigation. ClinChim Acta; 383, 30-40.

Diretrizes da Sociedade Brasileira de Cardiologia (2013). Atualização da Diretriz Brasileira de Dislipidemias e Prevenção da Aterosclerose. Arq Bras Cardiol. 109 (2Supl.1) p.1-76.

Drobitch, RK & Svensson, CK (1992). Therapeutic Drug Monitoring in Saliva An Update. Clin. Pharmacokinet. 23(5), 365-379.

Kensche, A, Reich, M, Kümmerer, K, Hannig, M & Hannig, C. (2013). Lipids in preventive dentistry. Clinical Oral Investigations. 17(3)669–685.

Kaufman, E & Lamster, IB. (2002). The diagnostic applications of saliva: A review. Crit Rev Oral Biol Med. 13, 197-212.

Karjalainen, S, Sewón, L, Soderling, E, Larsson, B, Johansson, I, Simell, O et al. (1997). Salivary cholesterol of healthy adults in rela¬tion to serum cholesterol concentration and oral health. J Dent Res; 76(10), 1637–43.

Lohe, VK, Degwekar, SS, Bhowate, RR & Kadu, RP. (2010).Dangore SB. Evaluation of correlation of serum lipid profile in patients with oral cancer and precancer and its association with tobacco abuse. J Oral Pathol Med. 39, 141-8.

Martins, MJJ. (2012). Os Lipídios na Prática Médica. Maceió: UNCISAL.

Moura, SAB, Medeiros, AMC, Costa, FRH, Moraes, PH & Oliveira Filho, AS. (2007). Valor Diagnóstico da Saliva em Doenças Orais e Sistêmicas: Uma Revisão de Literatura. Pesquisa Brasileira em Odontopediatria e Clínica Integrada. 7(2), 187–194.

Mussavira, S, Dharmalingam, M & Sukumaran, BO. (2015). Salivary glucose and antioxidant defense markers in type II diabetes mellitus. Turkish Journal of Medical Sciences. 45(1) 141–147.

Navazesh, M. (1993). Methods for collecting saliva. Annals of the New York Academy of Sciences. 694,72–77.

Nelson, DL & Cox, MM (2000). Lipids. In: Nelson, D.L.; Cox, M.M.; editors. Lehninger principles of biochemistry. 3 rd ed. New York: Worth Publishers. 363-88.

Patel, PS, Shah, MH, Jha, FP, Raval, GN, Rawal, RM, Patel, MM et al. (2004) Alterations in plasma lipid profile patterns in head and neck cancer and oral precancerous conditions. Indian J Cancer. 41, 25-31.

Pereira, AS, Shitsuka, DM, Parreira, FJ & Shitsuka, R. (2018). Metodologia da pesquisa científica. [e-book]. Santa Maria. Ed. UAB/NTE/UFSM. Disponível em: https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.

Subramaniam, P.; Sharma, A.;Kaje, K; (2015). Association of salivary triglycerides and cholesterol with dental caries in children with type 1 diabetes mellitus. SpecCareDentist. 35(3)120-122.

Singh, S, Ramesh, V, Premalatha, B, Prashad, KV & Ramadoss, K. (2013). Alterations in serum lipid profile patterns in oral cancer. J Nat Sci Biol Med. 4, 374-8.

Segal, A & Wong, DT. (2009). Salivary diagnostics: enchancing disease detection and making medicine better, Eur J Dent Educ. 12 (Suppl 1): 22–29.

Slomiany, BL, Zdebska, E, Murty, VL, Slomiany, A, Petropoulou, K & Mandel, ID. (1983). Lipid composition of human labial salivary gland secretions. Arch Oral Biol. 28, 711-4.

Tommasi, MH. (2013).Diagnóstico em: patologia bucal. p 283. 4 ed, Rio de Janeiro: Elsevier.

Vedanthan, R, Bansilal, S, Soto, AV, Kovacic, JC, Latina, J, Jaslow, R et al. (2016). Family-Based Approaches to Cardiovascular Health Promotion. Journal of the American College of Cardiology. 67(14),1725–1737.

Yoshizawa, JM, Schafer, CA, Schafer, JJ, Farrell, JJ, Paster, BJ & Wong, TW. (2013). Salivary Biomarkers: Toward Future Clinical and Diagnostic Utilities. 26(4), 781–791.

Published

20/05/2020

How to Cite

CRUZ, J. H. de A.; MOREIRA, I. C. de S.; ALVES, M. de F. V.; SATYRO, M. A. da S. A.; DINIZ, A. de F.; ANJOS, R. M. dos; GUÊNES, G. M. T.; PENHA, E. S. da; FIGUEIREDO, C. H. M. da C.; OLIVEIRA FILHO, A. A. de; ALVES, M. A. S. G. Comparison between triglycerides and cholesterol concentrations in saliva and blood. Research, Society and Development, [S. l.], v. 9, n. 7, p. e412974361, 2020. DOI: 10.33448/rsd-v9i7.4361. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/4361. Acesso em: 16 nov. 2024.

Issue

Section

Health Sciences