Eradication measures and products that inhibit in vitro mycelial growth of the fungus Neoscytalidium dimidiatum, the etiological agent of pitaya canker
DOI:
https://doi.org/10.33448/rsd-v13i3.45467Keywords:
Hylocerus sp.; Management; Fungicides; Fungi.Abstract
The objective of this work was to identify the causal agent of the circular necrotic lesion present in cladodes and pitaya fruits, to test in vitro different products with potential fungicidal effect and to evaluate the effect of different eradicating products on infected pruned cladodes. Fruits and cladodes with symptoms of pitaya canker were analyzed using a magnifying glass and microscope. Isolation in PDA and inoculation was also carried out in healthy cladodes to observe symptoms. The isolated fungus was placed to grow in PDA culture medium previously mixed with the products; Castor oil, Prevam, Biometal copper, Kellus immune, Primecur, Kaumin, Bombardeiro, Serenade, Bordamil, Dithane, Bravonil, Mertin, Rovral, Sumilex, Mythos, Cantus, Fluazinam Nortox, Comet, Cercobin, Forum, Socre Flex, Cabrio Top, Orkestra, Opera, Nativo, Fusion, Tridium, Priori Xtra and Evolution. The products were applied to infected cladodes; Quaternary ammonia, Fortalece, Bordamil, Calda sulfocaucica all in concentrations of 5% and control with the objective of observing sporulation, spore germination and decomposition time. Pitaya cladodes and fruits presented stem canker caused by Neoscytalidium dimidiatum. The products; Prevam, Bordamil, Dithane, Mertin, Rovral, Sumilex, Fluazinam Nortox, Comet, Socre Flex, Cabrio Top, Orkestra, Opera, Nativo, Fusion, Tridium, Priori Xtra and Evolution at the doses tested completely inhibited the mycelial growth of the N. dimidiatum fungus. The products; Bordamil®, Sulfocaucic solution, Quaternary Ammonia and Fortalece® in concentrations of 5% accelerated the decomposition of the treated cladode and reduced the formation and germination of N. dimidiatum conidia.
References
Ajitomi, A., Minoshima, A., Takushi, T., Truong, H. H., Ooshiro, A., Yamashiro, M., & Hirooka, Y. (2020). First report of mango (Mangifera indica) stem-end rot caused by two Diaporthe species and their susceptibility to procymidone. Journal of general plant pathology, 86, 237-244.
An, N. N., Thao, H. H. M., Yen, H. N. H., Hanh, N. T. D., HOA, N. L. H., TIEN, T. T. T., & VIET, P. T. (2020). Isolation, identification and characterization of bacterial antagonists of the dragon fruit fungal pathogen Neoscytalidium dimidiatum. Journal of Science and Technology-IUH, 44(02).
Andrade, J. N., dos Santos, D. D. B., Barbosa, M., & Batista, D. D. C. (2018). Eficiência de doses da mistura de fluxapiroxade e piraclostrobina no crescimento de Lasiodiplodia theobromae e Neoscytalidium dimidiatum.
Ayres, M. R., Billones‐Baaijens, R., Savocchia, S., Scott, E. S., & Sosnowski, M. R. (2022). Critical timing of fungicide application for pruning wound protection to control grapevine trunk diseases. Australian Journal of Grape and Wine Research, 28(1), 70-74.
Bastos, D. C., Pio, R., Scarpare Filho, J. A., Libardi, M. N., Almeida, L. F. P. D., Galuchi, T. P. D., & Bakker, S. T. (2006). Propagação da pitaya'vermelha'por estaquia. Ciência e Agrotecnologia, 30, 1106-1109.
Blum, M., Boehler, M., Randall, E. V. A., Young, V., Csukai, M., Kraus, S., & Fonne‐Pfister, R. A. Y. M. O. N. D. E. (2010). Mandipropamid targets the cellulose synthase‐like PiCesA3 to inhibit cell wall biosynthesis in the oomycete plant pathogen, Phytophthora infestans. Molecular plant pathology, 11(2), 227-243.
Crous, P. W., Slippers, B., Wingfield, M. J., Rheeder, J., Marasas, W. F., Philips, A. J., & Groenewald, J. Z. (2006). Phylogenetic lineages in the Botryosphaeriaceae. Studies in mycology, 55(1), 235-253.
Dy, K. S., Wonglom, P., Pornsuriya, C., & Sunpapao, A. (2022). Morphological, molecular identification and pathogenicity of Neoscytalidium dimidiatum causing stem canker of Hylocereus polyrhizus in southern Thailand. Plants, 11(4), 504.
Esguera, J. G., Balendres, M. A., & Paguntalan, D. P. (2024). Overview of the Sigatoka leaf spot complex in banana and its current management. Tropical Plants, 3(1).FALEIRO, F. G. Pitaia: a fruta que está conquistando o Brasil. 2022.
Fullerton, R. A., Sutherland, P. A., Rebstock, R. S., Hieu, N. T., Thu, N. N. A., Linh, D. T., & Van Hoa, N. (2018). The life cycle of dragon fruit canker caused by Neoscytalidium dimidiatum and implications for control. Management, 11.
Hashem, A. H., Abdelaziz, A. M., Hassanin, M. M., Al-Askar, A. A., AbdElgawad, H., & Attia, M. S. (2023). Potential impacts of clove essential oil nanoemulsion as bio fungicides against Neoscytalidium blight disease of Carum carvi L. Agronomy, 13(4), 1114.
Hong, C. F., Zhang, S., Gazis, R., Crane, J. H., & Wasielewski, J. (2020). Stem and Fruit Canker of Dragon Fruit in South Florida: PP355, 12/2019. EDIS, 2020(1).
Jian, Y., Chen, X., Ma, H., Zhang, C., Luo, Y., Jiang, J., & Yin, Y. (2023). Limonene formulation exhibited potential application in the control of mycelial growth and deoxynivalenol production in Fusarium graminearum. Frontiers in Microbiology, 14, 1161244.
Kasumin (2024): Bula de fungicida. 15 p.: https://www.adapar.pr.gov.br/sites/adapar/arquivos_restritos/files/documento/2020-10/kasumin1.pdf. Acesso em: 13 Mar. 2024.
Köche, J. C. (2016). Fundamentos de metodologia científica. Vozes.
Lan Guobing, He Zifu, Yu Lin, Tang Yafei, Li Zhenggang, Deng Mingguang e She Xiaoman (2019). Duração e testes de eficácia de controle de campo de 16 fungicidas contra podridão parda de pitaya. Guangdong Agricultural Sciences, 46(12) 95-101.
Luu, T. T. H., Le, T. L., Huynh, N., & Quintela-Alonso, P. (2021). Dragon fruit: A review of health benefits and nutrients and its sustainable development under climate changes in Vietnam. Czech Journal of Food Sciences, 39(2), 71-94.
Mohd, M. H., Salleh, B., & Zakaria, L. (2013). Identification and molecular characterizations of Neoscytalidium dimidiatum causing stem canker of red‐fleshed dragon fruit (Hylocereus polyrhizus) in Malaysia. Journal of Phytopathology, 161(11-12), 841-849.
Morillo Coronado, A. C., Manjarres Hernández, E. H., Pedreros Benavides, M. C., Sanabria Higuera, D. I., Lizarazo Forero, L. M., Morales Castaño, I. T., ... & Velásquez Arias, J. O. (2022). Plan de manejo tecnológico del cultivo de la pitahaya.
Nguyen, T. D., Phan, Q. K., & Do, A. D. (2023). Antagonistic activities of Trichoderma spp. isolates against Neoscytalidium dimidiatum causing brown spot disease on dragon fruit Hylocereus undatus. Journal of Applied Biology and Biotechnology, 12(1), 265-272.
Olivares, B. O., Vega, A., Calderón, M. A. R., Montenegro-Gracia, E., Araya-Almán, M., & Marys, E. (2022). Prediction of Banana Production Using Epidemiological Parameters of Black Sigatoka: An Application with Random Forest. Sustainability, 14 (21).
Pereira A. S. et al. (2018). Metodologia da pesquisa científica. UFSM.
Poni, S., Sabbatini, P., & Palliotti, A. (2022). Facing spring frost damage in grapevine: recent developments and the role of delayed winter pruning–a review. American Journal of Enology and Viticulture, 73(4), 211-226.
Ratanaprom, S., Nakkanong, K., Nualsri, C., Jiwanit, P., Rongsawat, T., & Woraathakorn, N. (2021). Overcoming encouragement of dragon fruit plant (Hylocereus undatus) against stem brown spot disease caused by Neoscytalidium dimidiatum using Bacillus subtilis combined with sodium bicarbonate. The plant pathology journal, 37(3), 205.
Sakçi, N., Şener, K. U. R. T., Uysal, A., Soylu, E. M., Merve, K. A. R. A., & Soylu, S. (2021). Identification and Pathogenicity of Neoscytalidium novaehollandiae, the Agent of Canker and Dieback in Almonds and In Vitro Activities of Some Fungicides. Yuzuncu Yıl University Journal of Agricultural Sciences, 32(1), 132-142.
Silva, J. V., Gasparotto, L., Blum, L. E. B., & Pinho, D. B. (2023). Primeiro relato de Neoscytalidium dimidiatum causando cancro em pitaya no Brasil. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 53., 2023, Brasília, DF, 2023. Anais 2023. Brasília, DF: Sociedade Brasileira de Fitopatologia, 2023.
Taguiam, J. D., Evallo, E., Bengoa, J., Maghirang, R., & Balendres, M. A. (2020). Susceptibility of the three dragon fruit species to stem canker and growth inhibition of Neoscytalidium dimidiatum by chemicals. Journal of Plant Pathology, 102, 1077-1084.
Uyen, D.T.K.; Campbell, J.; Hieu, N.T.; Van Hoa, N.; Fullerton, R. The Introduction of Gap and Quality System for Pitaya in Vietnam. Available online: https://ap.fftc.org.tw/article/1293 (accessed on 20 March 2024).
Yumbo Grefa, Y. (2022). Sensibilidad in vitro de Neoscytalidium spp. agente causal del cáncer de la pitahaya a fungicidas de diferentes modos de acción. Available online: http://dspace.espoch.edu.ec/handle/123456789/16551 (acessed on 15 March 2024).
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 Marcelo Loran de Oliveira Freitas; Diogo Aparecido de Carvalho; Rodrigo de Souza Almeida
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.