Development and description of a new minimally invasive surgical technique for oophorectomy in mice
DOI:
https://doi.org/10.33448/rsd-v13i8.46466Keywords:
Minor surgical procedures; Ovariectomy; Minimally invasive surgical procedures; Experimental development.Abstract
Introduction: Experimental animal models have an important role in the improvement of knowledge about diverse pathologies, being mice the main experimental animals, used to learn about those diseases. There’s a very important significance of oophorectomy in experimentation, and because of that we studied to develop a new technique based on minimally invasive principles to obtain better outcomes and fewer surgical risks. Objectives: Develop and describe a new minimally invasive surgical method using a hook to perform oophorectomy in mice, develop a new oophorectomy-specific hook and evaluate operating parameters. Methods: For the research were used 30 female Wistar mice, young adults, weighing between 200-250g. During the procedure two 0,5cm incisions were performed, where the proper hook was inserted to search the ovary, when found, it was repaired and ligated and the procedure was repeated on the other side. Results and Discussion: The new surgical technique, using the hook, demonstrated beneficial because it presented: 1) Smaller incision to access the ovaries 2) Lower surgical time 3) Good wound evolution in a 7 days period 4) 0% (zero percent) mortality rate and 5) good performance and recovery after surgery. Conclusion: The development of a new surgical hook for oophorectomy in mice, enabled a minimally invasive procedure with two 0,5 cm incision (smallest size in literature), also a consequent faster surgical time and better postoperative conditions, being developed a new surgical technique with better outcomes and less complications.
References
Babinski, M. A. (2012). Anatomia dos ovários: considerações clínico-patológicas. Acta Scientiae Medica. 5(2), 43-52.
Bennett, K., & Lewis, K. (2022). Sedation and anesthesia in rodents. Veterinary Clinics: Exotic Animal Practice, 25(1), 211-255.
Burger, J. W. A., Van't Riet, M., & Jeekel, J. (2002). Abdominal incisions: techniques and postoperative complications. Scandinavian Journal of Surgery, 91(4), 315-321.
Carvalho, G. D., Masseno, A. P. B., Zanini, M. S., Zanini, S. F., Porfírio, L. C., Machado, J. P., & Mauad, H. (2009). Avaliação clínica de ratos de laboratório (Rattus novergicus linhagem Wistar): parâmetros sanitários, biológicos e fisiológicos. Ceres. 56(1), 51-7.
Estrela, C. (2018). Metodologia Científica: Ciência, Ensino, Pesquisa. Editora Artes Médicas.
Ferretti, M., Cavani, F., Manni, P., Carnevale, G., Bertoni, L., Zavatti, M., & Palumbo, C. (2014). Ferutinin dose-dependent effects on uterus and mammary gland in ovariectomized rats. Journal of Histology & Histopathology. 29 (8): 1027-37.
Gargiulo, S., Greco, A., Gramanzini, M., Esposito, S., Affuso, A., Brunetti, A., & Vesce, G. (2012). Mice anesthesia, analgesia, and care, Part I: anesthetic considerations in preclinical research. ILAR Journal, 53(1), E55-E69.
Giardino, R., Fini, M., Giavaresi, G., Mongiorgi, R., Gnudi, S., & Zati, A. (1993). Experimental surgical model in osteoporosis study. Bollettino della Società italiana di biologia sperimentale, 69(7-8), 453-460.
Grantcharov, T. P., & Rosenberg, J. (2001). Vertical compared with transverse incisions in abdominal surgery. European Journal of Surgery, 167(4), 260-267.
Govindarajan, P., Wolfgang B., Thaqif El K., Kampschulte, M., Schlewitz, G., Huerter, B., Sommer, U., Lutz D., Ignatius, A., Bauer, N., Szalay, G., Wenisch, S., Lips, K. S., Schnettler, R., Langheinrich, A., & Heiss, C. (2014). Bone Matrix, Cellularity, and Structural Changes in a Rat Model with High-Turnover Osteoporosis Induced by Combined Ovariectomy and a Multiple-Deficient Diet. The American Journal of Pathology, 184(3), 765–777.
Harkness, J. E., & Wagner, J. E. (1993). Biologia e clínica de coelhos e roedores. Editora Roca.
Hartke, J. R. (1999). Preclinical development of agents for the treatment of osteoporosis. Toxicologic Pathology, 27(1), 143-147.
Høegh-Andersen, P., Tankó, L. B., Andersen, T. L., Lundberg, C. V., Mo, J. A., Heegaard, A. M., & Christgau, S. (2004). Ovariectomized rats as a model of postmenopausal osteoarthritis: validation and application. Arthritis Research & Therapy, 6, 1-12.
Khajuria, D. K., Razdan, R., & Mahapatra, D. R. (2012). Descrição de um novo método de ooforectomia em ratas. Revista Brasileira de Reumatologia, 52, 466-470.
Lasota, A., & Danowska-Klonowska, D. (2004). Experimental osteoporosis-different methods of ovariectomy in female white rats. Roczniki Akademii Medycznej w Białymstoku, 49(Suppl 1), 129-131.
Palma, J. A., Gavotto, A. C., & Villagra, S. (1983). Effects of Diethylstilbestrol, 17 Beta Estradiol, and Progesterone on Plasma Fibrinogen Levels in Rats Submitted to Tissue Injury (Laparotomy). Journal of Trauma and Acute Care Surgery, 23(2), 132-135.
Parhizkar, S., Ibrahim, R., & Latiff, L. A. (2008). Incision choice in laparatomy: a comparison of two incision techniques in ovariectomy of rats. World Applied Sciences Journal, 4(4), 537-40.
Park, S. B., Lee, Y. J., & Chung, C. K. (2010). Bone mineral density changes after ovariectomy in rats as an osteopenic model: stepwise description of double dorso-lateral approach. Journal of Korean Neurosurgical Society, 48(4), 309.
Pereira A. S. et al. (2018). Metodologia da pesquisa científica. UFSM.
Pinheiro, D. C. S. N., Favali, C. B. F., Filho, A. A. S., Silva, A. C. M., Filgueiras, T. M., & Lima, M. G. S. (2003). Parâmetros hematológicos de camundongos e ratos do biotério central da Universidade Federal do Ceará. Colégio Brasileiro de Experimentação Animal (COBEA).
Ribeiro, W. L. C., de Almeida, C. A. S., & Oliveira, A. C. A. (2023). Cirurgias experimentais e ponto final humanitário em biomodelos animais. Editora Atena, Ponta Grossa/PR.
Santos, M. R. V., Souza, V. H., Menezes, I. A. C., Bitencurt, J. L., Resende-Neto, J. M., Barreto, A. S., & Barbosa, A. P. O. (2010). Parâmetros bioquímicos, fisiológicos e morfológicos de ratos (Rattus novergicus linhagem Wistar) produzidos pelo Biotério Central da Universidade Federal de Sergipe. Scientia Plena, 6(10).
Turner, R. T., Maran, A., Lotinun, S., Hefferan, T., Evans, G. L., Zhang, M., & Sibonga, J. D. (2001). Animal models for osteoporosis. Reviews in Endocrine & Metabolic Disorders, 2(1), 117.
Yang, P., Hish, G., & Lester, P. A. (2023). Comparison of Systemic Extended-release Buprenorphine and Local Extended-release Bupivacaine-Meloxicam as Analgesics for Laparotomy in Mice. Journal of the American Association for Laboratory Animal Science, 62(5), 416–422.
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Copyright (c) 2024 Denise Padilha Abs de Almeida; Wedson Silveira Santos; Stephanie Caroline da Costa Ferreira; Gabriela de Gusmão Pedrosa Eugênio; Marco Antonio Sant’Anna Bezerra; Janyne Aline Correia de Lima Garcia; Anne Caroline de Jesus Oliveira; Gilsan Aparecida de Oliveira; Ana Carolina Medeiros de Almeida
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