Analysis of the genotoxic effect of alcohol consumption, using the micronucleus test in students at Cesumar University, Ponta Grossa
DOI:
https://doi.org/10.33448/rsd-v14i1.47975Keywords:
Genotoxic agents; Metanuclear changes; Cytogenetics.Abstract
The exposure to genotoxic substances, such as alcohol, can cause damage to genomic DNA, leading to various types of cellular alterations. The Micronucleis are DNA fragments that are separated of the nucleus resulting in cellular instability. Their presence may be associated with several diseases, such as various types of cancer, Alzheimer's and AIDS. For this reason, and considering the high consumption of alcohol by university students, the objective of this project is to study quantitatively and qualitatively the presence of micronuclei in cells and their relationship with alcohol, through the micronucleus test in students of Unicesumar. A questionnaire was elaborated based on the AUDIT test to select through life habits the appropriate candidates for this study and divided in two groups, 15 people in the exposed group and 15 in the control group. After the collection of biological material, oral mucosa, microscopic and statistical analysis of this anomaly was performed, through the Tukey test. After analysis, it was possible to observe the direct relationship that alcohol, as well as other genotoxic agents have with the mutations that occurred in the nuclei of the cells of the oral mucosa. This project involves research with human beings and, therefore, was approved by the ethics committee according to the Certificate of Presentation and Ethical Appreciation (CAAE): 59946422.1.0000.5539.
References
Álcool - OPAS/OMS (2020). Organização Pan-Americana de Saúde. Disponível em: https://www.paho.org/pt/topicos/alcool
Andrade, R. B., & Campos, N. A. (2020). Comparação da frequência de micronúcleos entre indivíduos fumantes e não fumantes: uma revisão sistemática. Jornal Brasileiro de Patologia e Medicina Laboratorial.
Babor, T. F., Higgins-Biddle, J. C., Saunders, J. B., Monteiro, M. G., & World Health Organization. (2001). AUDIT: the alcohol use disorders identification test: guidelines for use in primary health care (No. WHO/MSD/MSB/01.6 a). World Health Organization.
Barbon, F. J., et al. (2014). Micronúcleos em Fumantes e Etilistas. I Med.
Carrard, V. C., et al. (2017). Teste dos Micronúcleos - Um Biomarcador de Dano Genotóxico em Células Descamadas da Mucosa Bucal. Revista da Faculdade de Odontologia, 48(⅓), 77-81.
Díaz, A. C., Mora, E. S., & Herrera, A. (2013). Presencia de micronúcleos en células epiteliales de encías, como marcador de inestabilidad cromosomal: Revisión sistemática. Avances em Odontoestomatología, 29(2), 95-102.
Dietz, J., et al. (2000). Pesquisa de Micronúcleos na Mucosa Esofágica e sua Relação com Fatores de Risco ao Câncer de Esôfago. Revista da Associação Médica Brasileira.
Faria, L. E. M., & Braga, J. R. M. (2015). Aplicação do Teste de Micronúcleo para Avaliação de Potencial Genotóxico em Epitélio Oral de Estudantes Universitários. Revista Atualiza Saúde, 1(1).
Garcia, L. P., et al. (2020). Consumo de álcool durante a pandemia da COVID-19: uma reflexão necessária para o enfrentamento da situação. Cadernos de Saúde Pública.
Holland, N., et al. (2008). The micronucleus assay in human buccal cells as a tool for biomonitoring DNA damage: the HUMN Project perspective on current status and knowledge gaps. Mutation Research, 659, 93-108.
Homann, N., et al. (1997). High acetaldehyde levels in saliva after ethanol consumption: methodological aspects and pathogenetic implications. Carcinogenesis, 18(9), 1739-1743.
Kolling, D. J. (2006). Padronização in vitro da técnica do micronúcleo em células vero para detecção de genotoxicidade. Resumo de Iniciação Científica na UFSC.
Maciel, L. A. M., Silva, D. R., Silva, S. V., Castro, T., Costa, L. S., Trolly, T. S., Goes, I. N. C., Feitosa, S. B., & Sousa, A. L. P. (2019). Determinação da frequência de micronúcleos em células esfoliativas da mucosa oral em indivíduos fumantes e etilistas. Pubsaude, 2, a.006. https://dx.doi.org/10.31533/pubsaude2.a006
Martins, K. F., & Filho, J. B. (2003). Determinação da frequência de micronúcleos e outras alterações nucleares em células da mucosa bucal de indivíduos não-fumantes e fumantes. Revista da Faculdade de Ciências Médicas, 5, 43-53.
Miranda, L. A., Leal, A. L. M. L., Junior, R. G. dos S., Dias, C. V., Ferreira, I. G. A., Oliveira, N. B. D., Gratão, A. L. S. M., Nobre, B. R. S., Carvalho, R. S. de, Batista, B. R. A., Carvalho, J. M. S. de, Borges, Y. O. O. C., Vial, T. F., Monteiro , P. F. dos S., & Canuto, M. M. de G. (2024). Efeitos carcinogênicos e mutagênicos do consumo de álcool sobre o trato gastrointestinal superior: Uma revisão de literatura. Brazilian Journal of Implantology and Health Sciences, 6(5), 415–427. https://doi.org/10.36557/2674-8169.2024v6n5p415-427
Setúbal, A. M. G., Reis, S. R. A., Robinson, W. M., & Borges-Osório, M. R. (2005). Micronúcleo: um importante marcador biológico intermediário na prevenção do câncer bucal. Revista Odonto Ciência-Faculdade de Odontologia PUCRS, 20, 137-141.
Stich, H. F., Curtis, J. R., & Parida, B. B. (1982). Application of the micronucleus test to exfoliated cells of high cancer risk groups: tobacco chewers. International Journal of Cancer, 30, 553-559.
Thomas, P., Harvey, S., Gruner, T., & Fenech, M. (2008). The buccal cytome and micronucleus frequency is substantially altered in Down’s syndrome and normal ageing compared to young healthy controls. Mutation Research, 638, 37-47.
Tolbert, P. E., Shy, C. M., & Allen, J. W. (1991). Micronuclei and other nuclear anomalies in buccal smears: a field test in snuff users. American Journal of Epidemiology, 134, 840-850.
Tolbert, P. E., Shy, C. M., & Allen, J. W. (1992). Micronuclei and other nuclear anomalies in buccal smears: methods development. Mutation Research, 271, 69-77.
Torres, B. O., & Arias, R. L. F. (2023). Micronúcleos: Actualización del papel en la inestabilidad genética, inflamación, envejecimiento y cáncer. Revisión panorámica. Rev Biomed. 34(2):208-223.
Vieira, C., Aguiar, Z. S. T., & Souza, F. V. (2015). Tabagismo e sua relação com o câncer bucal: uma revisão de literatura. Revista Bionorte, 4(2).
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Bruna Meller Schmidt; Michele Andressa Vier Wolski
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
