Mutations of antiretroviral resistance and genetic diversity of HIV-1 in pregnant women attended at a reference maternity hospital in the State of Piauí, Brazil

Authors

DOI:

https://doi.org/10.33448/rsd-v9i9.6683

Keywords:

VIH-1; Pregnant women; HIV-1 ubtypes; Resistance mutations; Piauí.

Abstract

Objective: to identify HIV-1 subtypes, evaluate the presence of antiretroviral resistance (DRM) mutations and estimate the resistance level of the mutant strains detected in pregnant women attended at the reference maternity in Piauí, northeastern Brazil. Methodology: Of the 873 pregnant women, 17 infected with HIV-1 were selected to participate in this study. Genotyping of HIV-1 was performed using nucleotide sequencing followed by phylogenetic analyzes to determine the viral subtype, including identification using the COMET online tool. DRM were defined using the Calibrated Population Resistance Tool program. Additional analyzes were also performed to check drug resistance in the pre-treatment using the Stanford HIVdb Program. Results: Subtype B was detected in all pregnant women infected with HIV-1. The prevalence of DRM was 11.8%. The L90M and K103N mutations were identified, with high (nelfinavir and nevirapine) and intermediate (indinavir and saquinavir) resistance to different antiretrovirals. Conclusion: This study identified important genetic information on HIV-1 in pregnant women in Piauí, which can assist in directing control and prevention measures for this viral infection.

Author Biography

Evaldo Hipólito de Oliveira, Universidade Federal do Piauí

Centro de Ciências da Saúde
Curso de Farmácia

Disciplinas de Microbiologia Clínica e Imunologia Clínica

References

Afonso, J. M., et al. (2012). HIV-1 Genetic Diversity and Transmitted Drug Resistance Mutations among Patients from the North, Central and South Regions of Angola. PLOS ONE. 7(8), e42996.

Amorim, A., & Lima, Z. (2014). Avaliação de carga viral e linfócitos CD4+ em gestantes soropositivas em tratamento antirretroviral 18p. Centro de Ciências Biológicas e da Saúde, Universidade Estadual da Paraíba, Campina Grande.

Andrade, S. D., Sabidó, M., Monteiro, W. M., et al.: (2017). Drug resistance in antiretroviral‐naïve children newly diagnosed with HIV‐1 in Manaus, Amazonas. J Antimicrob Chemother. 72. 1774‐1783.

Anisimova, M., & Gascuel O. (2006). Approximate likelihood-ratio test for branches: A fast, accurate, and powerful alternative. Syst Biol. 55(4), 539-552.

Arruda, E., Simões, L., Sucupira, C., et al. (2011). Short communication: intermediate prevalence of HIV type 1 primary antiretroviral resistance in Ceará State, Northeast Brazil. AIDS Res Hum Retroviruses. 27(2), 153-156.

Bennett, D. E., Camacho, R. J., Otelea, D., et al. (2009). Drug resistance mutations for surveillance of transmitted HIV-1 drug-resistance: 2009 update. PLoS One. 2009; 4(3): e4724.

Brasil, Ministério da Saúde (2010). Recomendações para profilaxia da transmissão vertical do HIV e terapia antirretroviral em gestantes. Brasília: Ministério da Saúde, Secretaria de Vigilância em Saúde, Departamento de DST, Aids e Hepatites Virais. 172 p.

Brasil, Ministério da Saúde. (2017). Nota Informativa nº 007/2017- DDAHV/SVS/MS. Ministério da Saúde. Secretaria de Vigilância em Saúde. Brasília- DF.

Brasil, Ministério da Saúde (2018). Departamento de Vigilância, Prevenção e Controle das IST, do HIV/AIDS e das Hepatites Virais. Boletim epidemiológico HIV/AIDS 2018. http://www.aids.gov.br/pt-br/pub/2018/boletim-epidemiologico-hivaids-2018.

Camargo, R., & Strapasson, M. (2016). HIV no pré-natal: revisão integrativa da literatura (TCC). 16p. Unidade Acadêmica de Educação Continuada, Universidade do Vale do Rio dos Sinos, Porto Alegre.

Cardoso, L. P., Queiroz, B. B., Stefani, M. M. (2009). HIV-1 pol phylogenetic diversity and antiretroviral resistance mutations in treatment naïve patients from Central West Brazil. J Clin Virol. 46(2), 134-139.

Cardoso, L. P., da Silveira, A. A., Francisco, R. B., da Guarda, R. M. N., Stefani, M. M. (2011). Molecular characteristics of HIV type 1 infection among prisoners from Central Western Brazil. AIDS Res Hum Retroviruses. 27(12), 1349-1353.

Cavalcanti, A. M., Brito, A. M., Salustiano, D. M., et al. (2012). Primary resistance of HIV to antiretrovirals among individuals recently diagnosed at voluntary counselling and testing centres in the metropolitan region of Recife, Pernambuco. Mem Inst Oswaldo Cruz. 107(4), 450-457.

da Costa, L. M., Frade, P. C. R., Blandtt, L. D. S., et al. (2020). HIV-1 Genetic Diversity and Transmitted Drug Resistance Mutations in Female Sex Workers from a Brazilian Municipality in the Amazon Region. AIDS Res Hum Retroviruses. 2020. 36(2), 99-100.

De Lima, K. O. (2015). Características filogenéticas, epidemiológicas, laboratoriais e evolutivas dos subtipos B e não-B do HIV-1 em Pernambuco – Nordeste do Brasil. Tese do Programa de PósGraduação em Medicina Tropical do Centro de Ciências da Saúde da Universidade Federal de Pernambuco. Recife-PE.

de Medeiros, L. B., Lacerda, H. R., Cavalcanti, A. M., de Albuquerque, M. F. (2006). Primary resistance of human immunodeficiency virus type 1 in a reference center in Recife, Pernambuco, Brazil. Mem Inst Oswaldo Cruz. 101(8), 845-849.

da Silveira, A. A., Cardoso, L. P., Francisco, R. B., de Araújo, S. M. M. (2012). HIV type 1 molecular epidemiology in pol and gp41 genes among naive patients from Mato Grosso do Sul State, central western Brazil. AIDS Res Hum Retroviruses. 28(3), 304-307.

Dos Anjos, S. L., Divino, F., da Silva, R. M. O., et al.: (2016). HIV‐1 Genetic diversity and transmitted drug resistance in antiretroviral treatment‐naive individuals from Amapá state, Northern Brazil. AIDS Res Hum Retroviruses. 32, 373‐376.

Drake, A., et al. (2014). Incident HIV during Pregnancy and Postpartum and Risk of Mother-to-Child HIV Transmission: A Systematic Review and Meta-Analysis. PLOS Medicine, 11(2), 01-16.

Ferreira, A. S., Cardoso, L. P., Stefani, M. M. (2011). Moderate prevalence of transmitted drug resistance and high HIV-1 genetic diversity in patients from Mato Grosso State, Central Western Brazil. J Med Virol. 83(8), 1301-1307.

Friedrich, L., et al. (2016). Transmissão vertical do HIV: uma revisão sobre o tema. Boletim Científico de Pediatria, 5(3), 81-86, 2016.

Gifford, R. J., Liu, T. F., Rhee S.Y., et al. (2009). The calibrated population resistance tool: standardized genotypic estimation of transmitted HIV-1 drug resistance. Bioinformatics. 25(9), 1197-1198.

Guindon, S., Dufayard, J. F., Lefort, V., et al. (2010). New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol. 59(3), 307-321.

Iria, S. D. F., Torres, M. K. S., Avelino, M. E. S., et al.: (2019). HIV‐1 transmitted drug resistance mutations in recently diagnosed antiretroviral‐naive patients in Belém, Pará, Northern Brazil. AIDS Res Hum Retroviruses. 35, 121‐122.

Larsson, A. (2014). AliView: a fast and lightweight alignment viewer and editor for large datasets. Bioinformatics. 30(22), 3276-3278.

Leal, É., Arrais, C. R., Barreiros, M., et al. (2020). Characterization of HIV-1 genetic diversity and antiretroviral resistance in the state of Maranhão, Northeast Brazil. PLoS One. 2020;15(3), e0230878.

Lefort, V., Longueville, J. E., & Gascuel, O. (2017). SMS: Smart Model Selection in PhyML. Mol Biol Evol. 34(9), 2422-2424.

Lima, S., et al. (2017). HIV na gestação: pré-natal, parto e puerpério. Ciência & Saúde, 10(1), 56-61.

Pereira, A. S., et al. (2018). Metodologia da pesquisa científica. [e-book]. Santa Maria. Ed. UAB/NTE/UFSM. Recuperado de https://repositorio.ufsm.br/bitstream /handle/1/15824/Lic_ Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.

Verde, R. M. C. L., et al. (2020). Epidemiological characterization of human immunodeficiency virus type 1 in pregnant women of a maternity hospital in Piauí, Brazil. Research, Society and Development, Itabira, 9(4), e25942503.

Luz, R. P., & Eulálio, K. D. (2018). Resistência transmitida aos antirretrovirais em pacientes infectados pelo HIV-1 atendidos em um centro de referência. JCS HU-UFPI. 1(2), 51-62.

Moura, M. E., da Guarda, R. M. N., Lima, Y. A., Eulálio, K. D., Cardoso, L. P., Stefani, M. M. (2015). HIV-1 transmitted drug resistance and genetic diversity among patients from Piauí State, Northeast Brazil. J Med Virol. 87(5),798-806.

Pedroso, C., Queiroz, A. T., Alcântara, L. C., et al. (2007). High prevalence of primary antiretroviral resistance among HIV-1-infected adults and children in Bahia, a northeast state of Brazil. J Acquir Immune Defic Syndr. 45(2), 251-253.

Pimenta, A. T. M., & Quintana, S. M. (2015). Mutações de resistência em gestantes infectadas pelo HIV: uma revisão da literatura. Ciência & Saúde. 8(3), 137-145.

Rosa, M. C., Lobato, R. C., Alves, C. V. G., da Silva, N. M., Barral, M. F., & de Martinez, A. M. et al. (2015). Avaliação de fatores associados à transmissão vertical do HIV-1. J Pediatr (Rio J). 91(6), 523-8.

Shafer, R. W., & Schapiro, J. M. (2008). HIV-1 Drug Resistance Mutations: an Updated Framework for the Second Decade of HAART. AIDS Rev 10, 67-84.

Silva, S., et al. (2015). Rede de apoio a mulheres com hiv na prevenção da transmissão vertical: revisão integrativa. Rev Min Enferm, 19(2), 225-231, 2015.

Struck, D., Lawyer, G., Ternes, A. M., et al. (2014). COMET: adaptive context-based modeling for ultrafast HIV-1 subtype identification. Nucleic Acids Res. 42(18), e144.

World Health Organization (2017) World Health Organization HIV drug resistance report. http://www.who.int/hiv/pub/drugresistance/hivdr-report-2017/en/

Published

14/08/2020

How to Cite

Verde , R. M. C. L. ., Oliveira, E. H. de, Oliveira Filho, A. B. ., Costa, M. M. da ., & Pavinatto, A. . (2020). Mutations of antiretroviral resistance and genetic diversity of HIV-1 in pregnant women attended at a reference maternity hospital in the State of Piauí, Brazil. Research, Society and Development, 9(9), e160996683. https://doi.org/10.33448/rsd-v9i9.6683

Issue

Section

Health Sciences