Elaboration, physical-chemical and sensorial characterization of fermented kefir milk flavored with green fruits and added with inulin

Authors

DOI:

https://doi.org/10.33448/rsd-v9i9.7179

Keywords:

Dairy products; Functional food; Prebiotic; Probiotic; Starter culture.

Abstract

Kefir is a probiotic fermented milk that can have its functional appeal reinforced with the addition of prebiotic ingredients, such as inulin. The objective of the work was to evaluate the influence of the addition of inulin on the physical-chemical and sensory parameters of sweetened and flavored kefir formulations with green fruits, elaborated by two fermentative methods. Four kefir formulations were made: FS1 - Kefir with inulin, FS2 - Kefir without inulin (prepared with kefir grains), FS3 - Kefir with inulin, FS4 - Kefir without inulin (prepared with lyophilized starter culture). The addition of inulin did not influence the fermentation process of fermented milks, formulations FS1 and FS2 showed higher acidity and lower pH. All samples are in accordance with the legislation for protein and lipid content and the addition of inulin and green fruit preparation contributed to the increase in the solids content, reflecting in the moisture content, with an increase in the carbohydrate content and energy value. In the sensory analysis, all fermented milks had good acceptance, with scores above 6 for the texture attribute in formulations with the addition of inulin. This prebiotic also did not impart residual flavor and color change. Based on the results, it can be concluded that kefir made from lyophilized starter culture, added inulin and prepared from green fruits is a good option to meet the growing demand for functional foods in the market, with a greater standardization compared to formulations made with kefir grains.

Author Biographies

Danielle Fátima D’Angelis, Universidade Federal de Minas Gerais

Faculdade de Farmácia

Mestrado em Ciência de Alimentos

Aline Cristina Arruda Gonçalves, Universidade Federal de São João del Rei

Departamento de Engenharia de Alimentos

Análise Sensorial

Qualidade na Indústria de Alimentos

Vinícius Tadeu da Veiga Correia, Universidade Federal de Minas Gerais

Doutorando em Ciência de Alimentos

Faculdade de Farmácia

Cíntia Nanci Kobori, Universidade Federal de São João del Rei

Departamento de Engenharia de Alimentos

Desenvolvimento de Novos Produtos

Bioquímica de Alimentos

Tecnologia de Óleos e Gorduras

Juliana Cristina Sampaio Rigueira Ubaldo, Universidade Federal de São João del Rei - Campus Sete Lagoas

Departamento de Engenharia de Alimentos

References

AOAC. Association of Official Analytical Chemists. (2012). Official methods of analysis of the AOAC International. 19th edition. Washington: AOAC.

Araújo, N. G., Da Silva, J. B., Barbosa, I. M., & Macêdo, C. S. (2017). Influencia da concentração de polpa de goiaba na aceitação de fermentado de Kefir. Revista do Instituto de Laticínios Cândido Tostes, 72(4), 184–191. DOI: https://doi.org/10.14295/2238-6416.v72i4.552.

Bengoa, A. A., Iraporda, C., Acurcio, L. B., de Cicco Sandes, S. H., Costa, K., Moreira Guimarães, G., Esteves Arantes, R. M., Neumann, E., Cantini Nunes, Á., Nicoli, J. R., Garrote, G. L., & Abraham, A. G. (2019). “Physicochemical, immunomodulatory and safety aspects of milks fermented with Lactobacillus paracasei isolated from kefir”. Food Research International, 123, 48–55. DOI: https://doi.org/10.1016/j.foodres.2019.04.041.

Bourrie, B. C. T., Willing, B. P., & Cotter, P. D. (2016). The microbiota and health promoting characteristics of the fermented beverage kefir. Frontiers in Microbiology, 7, 1–17. https://doi.org/10.3389/fmicb.2016.00647.

Brasil (2007). Instrução Normativa n°46 de 23 de outubro de 2007. Aprova o regulamento técnico de identidade e qualidade de leites fermentados. Ministério da Agricultura, Pecuária e Abastecimento. Diário Oficial, Brasília, 24 Out. 2007, seção 1, 5.

Contim, L. S. R., Oliveira, I. M. A., & Cardoso Neto, J. (2018). Avaliação microbiológica, físico-química e aceitação sensorial do kefir com polpa de graviola. Revista do Instituto de Laticínios Cândido Tostes, 73(1), 1–9. DOI: https://doi.org/10.14295/2238-6416.v73i1.604.

Costa, N. M. B., & Rosa, C. de O. B. (2010). Alimentos funcionais - Componentes bioativos e efeitos fisiológicos. Rio de Janeiro: Editora Rubio.

Davras, F., Guzel-Seydim, Z. B., & Tas, T. K. (2018). Immunological effects of Kefir produced from Kefir grains versus starter cultures when fed to mice. Functional Foods in Health and Disease, 8(8), 367. DOI: https://doi.org/10.31989/ffhd.v8i8.533.

Da Silva, Á. G. F., Bessa, M. M., & Da Silva, J. R. (2017). Elaboração e caracterização físico-química e sensorial de iogurte light prebiótico adoçado com mel. Revista do Instituto de Laticínios Cândido Tostes, 72(2), 74–84. DOI: https://doi.org/10.14295/2238-6416.v72i2.577.

De Sainz, I., Redondo-Solano, M., Solano, G., & Ramírez, L. (2020). Short communication: Effect of different kefir grains on the attributes of kefir produced with milk from Costa Rica. Journal of Dairy Science, 103, 215–219. DOI: https://doi.org/10.3168/jds.2018-15970.

Demir, H. (2020). Comparison of traditional and commercial kefir microorganism compositions and inhibitory effects on certain pathogens. International Journal of Food Properties, 23(1), 375–386. DOI: https://doi.org/10.1080/10942912.2020.1733599.

Dos Reis, S. A., Da Conceição, L. L., Siqueira, N. P., Rosa, D. D., Da Silva, L. L., & Peluzio, M. do C. G. (2017). Review of the mechanisms of probiotic actions in the prevention of colorectal cancer. Nutrition Research, 37, 1–19. DOI: https://doi. org/10.1016/j.nutres.2016.11.009.

Dos Santos, D. C., De Oliveira Filho, J. G., Santana, A. C. A., De Freitas, B. S. M., Silva, F. G., Takeuchi, K. P., & Egea, M. B. (2019). Optimization of soymilk fermentation with kefir and the addition of inulin: Physicochemical, sensory and technological characteristics. LWT- Food Science and Technology, 104, 30–37. DOI: https://doi.org/10.1016/j.lwt.2019.01.030.

Ertekin, B., & Guzel-Seydim, Z. B. (2010). Effect of fat replacers on kefir quality. Journal of the Science of Food and Agriculture, 90, 543–548. DOI: https://doi.org/10.1002/jsfa.3855.

Ferreira, C. L. L. F. (2008). Produtos Lácteos Fermentados (Aspectos Bioquímicos e Tecnológicos). Viçosa: Editora UFV.

Garcia, L. V., Da Silva, F. R., Ribeiro, J. de A., Coêlho, M. D. G., & Urias, G. M. P. C. (2017). Avaliação da aceitabilidade de preparações do alimento probiótico kefir. Revista de Ciências da Saúde, 2(1), 16–21.

Gul, O., Mortas, M., Atalar, I., Dervisoglu, M., & Kahyaoglu, T. (2015). Manufacture and characterization of kefir made from cow and buffalo milk, using kefir grain and starter culture. Journal of Dairy Science, 98(3), 1517–1525. DOI: https://doi.org/10.3168/jds.2014-8755.

Hatmal, M. M., Nuirat, A., Zihlif, M. A., & Taha, M. O. (2018). Exploring the influence of culture conditions on kefir’s anticancer properties. Journal of Dairy Science, 101, 3771–3777. DOI: https://doi.org/10.3168/jds.2017-13539.

Helal, A., Rashid, N. N., Dyab, N. E., Al-Otaibi, M. M., & Alnemr, T. M. (2018). Enhanced Functional, Sensory, Microbial and Texture Properties of Low-Fat Set Yogurt Supplemented With High-Density Inulin. Journal of Food Processing & Beverages, 6(1), 1–11. DOI: https://doi.org/10.13188/2332-4104.1000020.

Hellwege, E. M., Czapla, S., Jahnke, A., Willmitzer, L., & Heyer, A. G. (2000). Transgenic potato (Solanum tuberosum) tubers synthesize the full spectrum of inulin molecules naturally occurring in globe artichoke (Cynara scolymus) roots. Proceedings of the National Academy of Sciences of the United States of America, 97(15), 8699–8704. DOI: https://doi.org/10.1073/pnas.150043797.

Khodear, M. M., Zayan, A. F., Tammam, A. A., & Mohran, M. A. (2018). Influence of Adding Inulin as a Fat Replacer on the Characteristics of Yoghurt. Journal of Food and Dairy Sciences, 9(1), 13–17. DOI: https://doi.org/10.21608/jfds.2018.35150.

Kim, D. H., Jeong, D., Song, K. Y., & Seo, K. H. (2018). Comparison of traditional and backslopping methods for kefir fermentation based on physicochemical and microbiological characteristics. LWT- Food Science and Technology, 97, 503–507. DOI: https://doi.org/10.1016/j.lwt.2018.07.023.

Kim, D. H., Kim, H., Jeong, D., Kang, I. B., Chon, J. W., Kim, H. S., Song, K. Y., & Seo, K. H. (2017). Kefir alleviates obesity and hepatic steatosis in high-fat diet-fed mice by modulation of gut microbiota and mycobiota: targeted and untargeted community analysis with correlation of biomarkers. The Journal of Nutritional Biochemistry, 44, 35–43. DOI: https://doi.org/10.1016/j.jnutbio.2017.02.014.

Maestri, B., Hererra, L., Silva, N. K., Ribeiro, D. H. B., & Chaves, A. C. S. D. (2014). Avaliação do impacto da adição de inulina e de maçã em leite fermentado probiótico concentrado. Brazilian Journal of Food Technology, 17(1), 58–66.

Manning, T. S., & Gibson, G. R. (2004). Prebiotics. Best Practice & Research Clinical Gastroenterology, 18(2), 287–298. DOI: https://doi.org/10.1016/j.bpg.2003.10.008.

Meilgaard, M., Civille, G. V., & Carr, B. T. (1999). Sensory evaluation techniques. 3 ed. Boca Raton: CRC.

Montanuci, F. D., Pimentel, T. C., Garcia, S., & Prudencio, S. H. (2012). Effect of starter culture and inulin addition on microbial viability, texture, and chemical characteristics of whole or skim milk Kefir. Food Science and Technology, 32(4), 850–861. DOI: https://doi.org/10.1590/s0101-20612012005000119.

Munday, H. S., & Bagley, L. (2017). The contribution of food science to nutrition science through reformulation in the last 50 years and into the future. Nutrition Bulletin, 42, 321–330. DOI: https://doi.org/10.1111/nbu.12286.

Munhoz, C. L., Hirata, V. G., Fria, S. B., & Ferreira, T. H. B. (2019). Elaboração de iogurtes de açaí (Euterpe Oleracea) com adição de prebióticos. Global Science and Technology, 12(3), 20–31.

Nielsen, B., Gürakan, G. C., & Ünlü, G. (2014). Kefir: A Multifaceted Fermented Dairy Product. Probiotics and Antimicrobial Proteins, 6, 123–135. DOI: https://doi.org/10.1007/s12602-014-9168-0.

O’Brien, K., Boeneke, C., Prinyawiwatkul, W., Lisano, J., Shackelford, D., Reeves, K., Christensen, M., Hayward, R., Ordonez, K. C., & Stewart, L. K. (2017). Short communication: Sensory analysis of a kefir product designed for active cancer survivors. Journal of Dairy Science, 100, 4349–4353. DOI: https://doi.org/10.3168/jds.2016-12320.

Pattaro, L., Silva, J. A. G. e, Farias, L. C. B., Medeiros, J. S., Teixeira, P. C., Da Cunha, J. V. T., More, J. C. R. S., De Almeida, T. V., Nicolau, E. S., & Da Silva, M. A. P. (2020). Análises físico-químicas e sensoriais de smoothies do leite de diferentes espécies fermentado por kefir, saborizados com banana e maçã. Research, Society and Development, 9(5), e112953145. DOI: https://doi.org/10.33448/rsd-v9i5.3145.

Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa científica. [e-book]. Santa Maria. Ed. UAB/NTE/UFSM. Recuperar de https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.

Pimenta, F. S., Luaces-Regueira, M., Ton, A. M. M., Campagnaro, B. P., Campos-Toimil, M., Pereira, T. M. C., & Vasquez, E. C. (2018). Mechanisms of Action of Kefir in Chronic Cardiovascular and Metabolic Diseases. Cellular Physiology and Biochemistry, 48, 1901–1914. DOI: https://doi.org/10.1159/000492511.

Pinto, V. R. A., Teixeira, C. G., Lima, T. S., Prata, E. R. B. de A., Vidigal, M. C. T. R., Martins, E., Perrone, Í. T., & De Carvalho, A. F. (2020). Health beliefs towards kefir correlate with emotion and attitude: A study using an emoji scale in Brazil. Food Research International, 129, 1–19. https://doi.org/10.1016/j.foodres.2019.108833.

Rubel, I. A., Pérez, E. E., Genovese, D. B., & Manrique, G. D. (2014). In vitro prebiotic activity of inulin-rich carbohydrates extracted from Jerusalem artichoke (Helianthus tuberosus L.) tubers at different storage times by Lactobacillus paracasei. Food Research International, 62, 59–65. DOI: https://doi.org/10.1016/j.foodres.2014.02.024.

Saeed, M., Yasmin, I., Pasha, I., Randhawa, M. A., Khan, M. I., Shabbir, M. A., & Khan, W. A. (2015). Potential application of inulin in food industry; A review. Pakistan Journal of Food Sciences, 25(3), 110–116.

Sarwar, A., Aziz, T., Al-Dalali, S., Zhao, X., Zhang, J., Ud Din, J., Chen, C., Cao, Y., & Yang, Z. (2019). Physicochemical and Microbiological Properties of Synbiotic Yogurt Made with Probiotic Yeast Saccharomyces boulardii in Combination with Inulin. Foods, 8(468), 1–18. DOI: https://doi.org/10.3390/foods8100468.

Published

16/08/2020

How to Cite

D’Angelis, D. F., Gonçalves, A. C. A. ., Correia, V. T. da V., Kobori, C. N., & Ubaldo, J. C. S. R. (2020). Elaboration, physical-chemical and sensorial characterization of fermented kefir milk flavored with green fruits and added with inulin. Research, Society and Development, 9(9), e246997179. https://doi.org/10.33448/rsd-v9i9.7179

Issue

Section

Agrarian and Biological Sciences