Analysis of biomechanical properties of tibias after bone failure and ozone treatment in rats
Keywords:Tibias; Biomechanical; Ozone; Resistance.
Introduction: Ozone is a potent antioxidant that acts as a precursor of various radicals, being indicated as a powerful therapy, assisting in the process of tissue healing. Objectives: The proposal of this study was to analyze material and structural properties via mechanical testing in tibias after application of ozone in bone defects produced surgically. Methods: Ten male 40-day old albino Wistar rats have been used, divided in two groups: control group and ozone group, this last one being treated with ozonized water in 25 µg/mL of concentration until the day of euthanasia. Trichotomy and longitudinal incision was conducted in the animals’ leg skin, exposing the tibia’s diaphysis of both antimers, and with help of a high rotation pen a flaw has been produced on the bone. After 60 days of surgery the animals were euthanized, and tibias were collected for biomechanical analysis. Results: The results of the biomechanical properties – structural and material – evidenced significant interactions through exposure to ozone, showing a diminished bone resistance in animals from the control group, observed by the decrease of the maximum force (N) needed to rupture the bone when compared to the value needed to break the bones of the animals from the ozone group, and the analysis of the morphometrical properties did not show any difference between both experimental groups. Conclusion: The use of ozone did not alter the morphological structures of the tibias, and the group which used ozone presented more resistance during mechanical testings, because the maximum force for the rupture of tibia was greater in this group.
Agrillo A., Petrucci, M. T., Tedaldi, M., et al. New therapeutic protocol in the treatment of avascular necrosis of the jaws. J Craniofac Sur 2006. 17(6), 1080-2.
Akhter, M. P., Cullen, D. M., Gong, G., et al. Bone biomechanical properties in prostaglandin EP1 and EP2 knockout mice. Bone. 2001. 29, 121-125.
Alpan, A. L., Toker, H., & Ozer, H. Ozone Therapy Enhances Osseous Healing in Rats With Diabetes With Calvarial Defects: A Morphometric and Immunohistochemical Study. J Periodontol 2016. 87(8), 982-9.
Batista, A. D., Mesa, M. G., Manresa, C. P., et al. Efecto Del ozono sobre La activación plaquetaria em pacientes diabéticos tratados con ozonoterapia: informe preliminar. Rer Cubana invest Biomed 2001. 20(1),45-7.
Baysan, A., Whiley, R. A., & Lynch, E. Antimicrobial effect of a novel ozone- generating device on cicro-organisms associated with primary root carious lesions in vitro. Caries Res 2000. 34(6),498-50.
Bocci, V. A. Comparative clinical studies between HOT and ozonetherapy in selected chronic pathologies are urgently needed. Arch Med Res 2006. 37, 919.
Bocci, V. A. Is it true that ozone is always toxic? The endo f a dogma. Toxicol Appl Pharmaco 2006. 216,493-504.
Bocci, V. A. Ozone: a new medical drug. (2nd ed.). Springer: The Netherlands; 2005.
Bocci, V. A. Physical-chemical properties of ozone. Natural production of ozone. The toxicology of ozone. In: Ozone, a new medical drug. Italy: Springer; 2005. 5-8.
Bocci ,V. A. (2006). Scientific and medical aspects of ozone therapy: state of the art. Arch Med Res. 37:425-35.
Buliés, J. C. E., Díaz, O. V., Rauder, R. S., et al. Resultados terapêuticos em La osteoartritis de la rodilla com infiltraciones de ozono. Rev Cubana Invest Biomed 1997; 16(2),124-32.
Buliés, J. C. E. Una solución para exposiciones óseas postraumáticas: asociación de injerto de epiplon mayor com ozonoterapia. Rev. Cubana Invest Biomed.1996. 15(2), 1-9.
Cardoso, C. C., Carvalho, J. C. T., Ovando, E. C., et al. Action of ozonized water in preclinical inflammatory models. Pharmacol Res 2000. 42(1), 51-4.
FELASA - Federacion de Asociaciones Europeas de las Ciencias de Animal de Laboratorio. Recomendaciones para la eutanasia de los animales de experimentacion. Retrieved from http://www.hulp.es/secal/secal.html.
Garcia, J. A. D., & Souza, A. L. T., & Cruz, L. H. C., et al. Effects of ethanol consumption and alcohol detoxification on the biomechanics and morphology the bone in rat femurs. Brazilian Journal of Biology (Online), 73, 1-8, 2015
Gent, J. F., Trichr, E. W., Holford, T. R., et al. Association of low-level ozone and fine partocles with respiratory symptoms in children with asthma. JAMA 2003. 290(14),1859-67.
Gregori, C., Campos, A. C. Cirurgia buço-dento-alveolar. (2nd ed.) São Paulo: Sarvier, 2004: 140-58.
Grootveld, M., Baysan, A., Sidüqui, N., et al. History of the clinical applications of ozone. In: E L, editor. London: Quintessence Publishing CO; 2004a . 23-30.
Grootveld, M., Silwood, C., Sim, J., et al. Safety aspects regarding the therapeutic applications of ozone and ozonated culinary oils in medicine and dentistry. In: Lynch E. Ozone: The revolution in dentistry. United Kingdom: Quintessence Publishing Books; 2004. 31-8.
Huang, T. H., Lin, S. C., Chang, F. L., et al. Effects of different exercise modes on mineralization, structure, and biomechanical properties of growing bone. J Appl Physiol 2003, 95, 300-307.
Kan, B., Sencimen, M., Bayar, G. R., et al. Histomorphometric and microtomographic evaluation of the effects of hyperbaric oxygen and systemic ozone, used alone and in combination, on calvarial defect healing in rats. J Oral Maxillofac Surg. 2015. 73(6),1231.e1-10.
Kandler, B., Maitz, P., Ficsher, M. B., et al. Plateles can neutralize hydrogen peroxide in na acue toxicity model with cells involved in granulation tissue formation. Bone 2005; 36, 671-7.
Kim, H. S., Noh, S., Han, Y. W., et al. Therapeutic effects of topical application of ozone on acute cutaneous wound healing. J Korean Med Sci. 2009; 24, 368-74.
Kwon, Y. B., Kim, H. W., Roh, D. H., et al. Topical application of epidemal growth factor accelerates wound healing by myofibroblast proliferation and collagen synthesis in rats. J Vet Sci 2006; 7(2), 105-9.
León Fernández, O. S., Viebahn-Haensler, R., Cabreja, G. L., et al. Medical ozone increases methotrexate clinical response and improves cellular redox balance in patients with rheumatoid arthritis. Eur J Pharmacol. 2016; 789, 313-8.
Lincheta, L. F., Simón, R. D., Cepero, S. M., et al. Solución para la epidermofitosis de lós pies em integrantes de lãs Fuerzas Armandas Revolucionarias. Rev. Cubana Med Milit 2000; 29(2), 98-102.
Martínez-Sánchez, G., Al-Dalain, S. M., Menéndez, S., et al. Therapeutic efficacy of ozone in patients with diabetic foot. Europ J Pharmacol 2005. 523, 151-61.
Palencia, G., Teixeira, F., Ortiz, A., et al. Detrimental effects of malnutrition on the damage induced by alcoholism: a study of animal models that simulate chronic alcoholism and malnutrition of large human groups. J Stud Alcohol 1994. 55(1), 113-20.
Re, L., Mawsouf, M. N., Menéndez, S., et al. Ozone therapy: clinical and basic evidence of is therapeutic potencial. Arch Med Res 2008. 39,17-26.
Samee, M., Kasugai, S., Kondo, H., et al. Bone morphogenetic protein-2 (BMP-2) and vascular endothelial growth factor (VEGF) transfection to human periosteal cells enhances osteoblast differentiation and bone formation. J Pharmacol Sci. 2008. 108,18-31.
Siqueira, J. F., Rôças, I. N., Cardoso, C. C., et al. Efeitos antibacterianos de um novo medicamento- o óleo ozonizado – comparados às pastas de hidróxido de cálcio. Rev Bras Odontol 2000. 57(4),252-6,
Soares, E. A., Novaes, R. D., Nakagaki, W. R., et al. Metabolic and structural bone disturbances induced by hyperlipidic diet in mice treated with simvastatin. Int J Exp Pathol 2015. 96(4),261-8.
Soares, E. V., Fávaro, W. J., Cagnon, V. H., et al. Effects of alcohol and nicotine on the mechanical resistance of bone and bone neoformation around hydroxyapatite implant. J Bone Miner Metab 2010. 28(1), 101-7.
Svendsen, P., & Hau, J. Handbook of laboratory animal science. Boca Raton: CRC Press; 1984.
Veranes, X. G., Nápoles, Y. L., & Hechavarría, I. C., et al. Resultados de lós costos em ozonoterapia. Rev Cubana Enfermer 1999;15(2), 104-8.
Weisbroth, S. H., Paganelli, R. G., & Salvia, M. Evalution of a disposable system during shipment of laboratory rats and mice. Lab Anim Sci 1977. 27(2), 186-94.
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Copyright (c) 2020 Alexadre Aniceto Rodrigues; Thiago Donizeth da Silva; Evelise Aline Soares; José Antônio Dias Garcia; Flavia da Ré Guerra; Fábio Alexandre Vieira Junior; Jordan Ribeiro dos Santos Souza; Roberto Conde Santos; Alessandra Esteves; Wagner Rossi Costa Júnior; Maria Eduarda Brito Araújo
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