Reaction of commercial cultivars of common bean to fusarium oxysporum f. sp. phaseoli

Authors

DOI:

https://doi.org/10.33448/rsd-v9i9.7506

Keywords:

Phaseolus vulgaris; Fusarium wilt; Resistance; Pathogenic variability.

Abstract

Common beans (Phaseolus vulgaris L.) are grown throughout the country and represent the main source of vegetable protein in the Brazilian diet. However, its production is strongly affected by biotic and abiotic factors, especially by diseases. Fusarium wilt, a disease caused by the fungus Fusarium oxysporum f. sp. phaseoli (Fop), is among the main diseases of this culture. The most efficient method for its control is the use of resistant cultivars, however, the lack of knowledge of the genetic and physiological variability of the pathogen hinders the development of resistant cultivars by breeding programs. In this sense, the objective of the present work was to characterize the reaction of the main commercial cultivars of common bean regarding resistance to different isolates of Fop. The bank of monosporic isolates was obtained from bean plants with symptoms of the disease in producing areas. The isolates were identified morphologically and molecularly by PCR. 14 commercial bean cultivars were inoculated with 5 distinct monosporic isolates and evaluated for wilt severity using the scale proposed by CIAT. In general, the commercial common bean cultivars evaluated showed resistance and intermediate resistance reactions to the studied Fop isolates, suggesting that possibly the PCR-SCAR marker was not efficient in the diagnosis of pathogen virulance and that the tested cultivars may have been unintentionally improved for Fusarium wilt resistance.

Author Biographies

Mariana Magesto de Negreiros, Universidade Estadual de Maringá

Departamento de Agronomia, UENP, campus Luiz Meneghel

Anderson Roberto Benedetti, Universidade de São Paulo

Departamento de Biologia, UENP, campus Luiz Meneghel

Bruna Martins Carvalho, Universidade Estadual do Norte do Paraná

Departamento de Agronomia, UENP, campus Luiz Meneghel

Aline Garcia da Silva, Universidade Estadual do Norte do Paraná

Departamento de Agronomia - UENP, campus Luiz Meneghel

João Pereira Torres, Universidade Estadual do Norte do Paraná

Departamento de Agronomia - UENP, campus Luiz Meneghel

References

Alves-Santos, F.M.; Benito, E.P.; Eslava, A.P.; Díaz-Mínguez, J.M. (2002). Pathogenicity and race characterization of Fusarium oxysporum f. sp. phaseoli isolates from Spain and Greece. Plant Pathology, 51 (5), 605–611. doi: 10.1046/j.1365-3059.2002.00745.x

Arif, M.; Chawla, S.; Zaidi, M.W.; Rayar, J.K.; Variar, M.; Singh, U.S. (2012). Development of specific primers for genus Fusarium and F. solani using rDNA sub-unit and transcription elongation factor (TEF-1α) gene. African Journal of Biotechnology, 11 (2), 444-447. doi: 10.5897/AJB10.489

Borba, M. C., Garcés-Fiallos, F. R., Stadnik, M. J. (2017). Reactions of black bean seedlings and adult plants to infection by Fusarium oxysporum f. sp. Phaseoli. Crop Protection, 96, 221-227. doi: 10.1016/j.cropro.2017.02.019

Bruns, T. D., Fogel, R., Taylor, J. W. (1990). Amplification and Sequencing of DNA from fungal herbarium specimens. Mycologia, 82 (2), 75-185. doi: 10.2307/3759846

Cândida, D.V.; Costa, J.G.; Rava, C.A.; Carneiro, M.S. (2009). Controle genético da murcha de fusário (Fusarium oxysporum) em feijoeiro comum. Tropical Plant Pathology, 34, 379-384. doi: 10.1590/S1982-56762009000600003

CONAB, Companhia Nacional de Abastecimento. (2018). Perspectivas para a Agropecuária. Obtido, em https://www.conab.gov.br/perspectivas-para-a-agropecuaria

Costa, H., Ferrão, M. A. G., Ventura, J. A., Liberato, J. R., Pacova, B. E. V. (2003). Murcha de Fusarium do feijoeiro. EMCAPA (Comunicado Técnico, 69). Obtido, em https://biblioteca.incaper.es.gov.br/digital/bitstream/item/1368/1/BRT-comunicadotecnico-n69-Emcapa.pdf

Costa, J. G. C.; Rava, C, A.; Puríssimo, J. D. (2007). Obtenção de linhagens de feijoeiro comum resistentes à murcha-de-fusário. Revista Ceres, 54, 447-542. Obtido em http://www.ceres.ufv.br/ojs/index.php/ceres/article/view/3252/1147

Czislowski, E.; Smith, S. F.; Zander, M.; O’neill, W. T.; Meldrum, R. A.; Tran-nguyen, L. T. T.; Batley, J.; Aitken, E. A. B. (2018). Investigation of the diversity of effector genes in the banana pathogen, Fusarium oxysporum f. sp. cubense, reveals evidence of horizontal gene transfer. Molecular Plant Pathology, 19 (5), 1155–1171. doi: 10.1111/mpp.12594

Cruz, A. F., Silva, L. F., Sousa, T. V., Nicoli, A., De Paula Junior, T. J., Caixeta, E. T., Zambolim, L. (2018). Molecular diversity in Fusarium oxysporum isolates from common bean fields in Brazil. European Journal of Plant Pathology, 152, 343–354. doi: 10.1007/s10658-018-1479-7

Henrique, F. H., Carbonell, S. A. M., Ito, M. F., Gonçalves, J. G. R., Sasseron, G. R., Chiorato, A. F. (2015). Classification of physiological races of Fusarium oxysporum f. sp. phaseoli in common bean. Bragantia, 74 (1), 84–92. doi: 10.1590/1678-4499.0265

Jiménez-Gasco, M. M., Milgroom, M. G., Jiménez-Díaz, R. M. (2002). Gene genealogies support Fusarium oxysporum f. sp. ciceris as a monophyletic group. Plant Pathology, 51, 72-77.

Kane, J.; Summerbell, R.; Sigler, L.; Krajden, S.; Land, G. (1997). Laboratory handbook of Dermatophytes: A Clinical Guide and Laboratory Manual of Dermatophytes and Other Filamentous Fungi. Belmont, USA: Star Publishing Co.

Kristensen, R., Torp, M., Kosiak, B., Holst-Jensen, A. (2005). Phylogeny and toxigenic potential is correlated in Fusarium species as revealed by partial translation elongation factor 1 alpha gene sequences. Mycological research, 109, 173-186. doi: 10.1017/S0953756204002114

Lanubile, A., Ellis, M. L., Marrocco, A., Munkvold, G. P. (2016). Association of effector Six6 with vascular wilt symptoms caused by Fusarium oxysporum on soybean. Phytopathology, 106, 1404-1412. doi: 10.1094/PHYTO-03-16-0118-R

Leitão, S. T., Malosetti, M., Song, Q., Eeuwijk, F. V., Rubiales, D., Patto, M. C. V. (2020). Natural variation in Portuguese common bean germplasm reveals new sources of resistance against Fusarium oxysoporum f. sp. phaseoli and resistance-associated candidate genes. Phytopathology, 110(3), 663-647. doi: 10.1094/PHYTO-06-19-0207-R

Leslie, F. J. & Summerell, A.B. (2006). The Fusarium Laboratory Manual. Sydney, Austrália: Blackwell Publishing.

Lievens, B., Rep, M., Thomma, B. P. H. J. (2008). Recent developments in the molecular discrimination of formae speciales of Fusarium oxysporum. Pest Managment Science, 64, 781-788. doi: 10.1002/ps.1564

Ma, L.J., Van Der Does, H.C., Borkovich, K.A., Coleman, J.J., Daboussi, M.J., Di Pietro, A. (2010). Dufresne, M., Freitag, M., Grabherr, M., Henrissat, B., and Houterman, P.M. Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium. Nature, 464, 367–373. doi: 10.1038/nature08850

Menezes, M. & Silva-Hanlin, D.M.W. (1997). Guia prático para fungos fitopatogênicos. Recife, Brasil: UFRPE.

Meziadi, C.; Richard, M.M.S.; Derquennes, A.; Thareau, V.; Blanchet, S.; Gratias, A.; Pflieger, S., Geffroy, V. (2015). Development of molecular markers linked to disease resistance genes in common bean based on whole genome sequence. Plant Science, 242, 352-357. doi: 10.1016/j.plantsci.2015.09.006

Ming, Y. N., Lin, P. C., Yu, T. F. (1966). Heterokaryosis in Fusarium fujikuroi (Saw.) Wr. Scientia Sinica Online, 15 (3), 371:378. Recuperado de https://europepmc.org/article/med/5933025

Möller, P. A., Wendland, A., Cortes, M. V. C. B., Pereira, R. J., Lobo Junior, M., Melo, L. C., Pereira, H. S., Costa, J. G. C. (2011). Repositório de informação tecnológica da Embrapa.

Nascimento, S.R.C.; Maringoni, A.C.; Kurozawa, C. (1995). Comportamento de variedades e linhagens de feijoeiro ao Fusaium oxysporum f. sp. phaseoli. Fitopatologia Brasileira, 20 (3) 458-463. Recuperado de https://www.scielo.br/scielo.php?script=sci_ nlinks&ref=000103&pid=S1413-7054201100050001100011&lng=en

Niño‐Sánchez, J.; Casado‐Del Castillo, V.; Tello, V.; De Veja-Bartol, J.J; Ramos, B.; Sukno, S.A.; Mínguez, J.M.D. (2016). The FTF gene family regulates virulence and expression of SIX effectors in Fusarium oxysporum, Molecular Plant Pathology, 17(7), 1124-1139. doi: 10.1111/mpp.12373

O’donnell, K.; Gueidan, C.; Sink, S.; Johnston, P.R.; Crous, P.W.; Glenn, A.; Riley, R.; Zitomer, N.C.; Colyer, P.; Waalwijk, C.; Van Der Lee, T.; Moretti, A.; Kang, S.; Kim, H-S.; Geiser, D.M.; Juba, J.H.; Baayen, R.P.; Cromey, M.G.; Bithell, S.; Sutton, D.A.; Skovgaard, K.; Ploetz, R.; Kistler, H.C.; Elliott, M.; Davis, M.; Sarver, B.A.J. (2009). A two-locus DNA sequence database for typing plant and human pathogens within the Fusarium oxysporum species complex. Fungal Genetics and Biology, 46, 936-948. doi: 10.1016/j.fgb.2009.08.006

Pastor-Corrales, M. A. & Abawi, G. S. (1987). Reactions of selected bean germplasms to infection by Fusarium oxysporum f. sp. phaseoli. Plant Disease, 71, 990-993. Recuperado de https://www.apsnet.org/publications/plantdisease/backissues/Documents/1987Articles/PlantDisease71n11_990.PDF

Paula Júnior, T. J.; Vieira, R. F.; Teixeira, H.; Lobo Junior, M.; Wendland, A. (2015). Doenças do feijoeiro: estratégias integradas de manejo. In J. Carneiro, T. Paula Júnior; A. Borém (Eds.), Feijão: do plantio à colheita (pp. 270-299). Viçosa, Brasil: Editora UFV.

Pereira, M.J.Z.; Ramalho, M.A.P.; Abreu, A.F.B. (2009). Inheritance of resistance to Fusarium oxysporum f. sp. phaseoli Brazilian race 2 in common bean. Scientia Agricola, 66, 788-792. doi: 10.1590/S0103-90162009000600010

Ramos, B., Alves-Santos, F. M., García-Sánchez, A., Martín-Rodrigues, N., Eslava, A. P., Díaz-Mínguez, J. M. (2007). The gene coding for a new transcription factor (ftf1) of Fusarium oxysporum is only expressed during infection of common bean. Science Direct, 44, 864-876. doi: 10.1016/j.fgb.2007.03.003

Ribeiro, R. L. D. & Hagedorn, D. J. (1979a). Inheritance of resistance in beans to Fusarium oxysporum f. sp. phaseoli. Phytopathology, 69, 859-61. doi: 10.1590/S0103-90162009000600010

Ribeiro, R. L. D. & Hagedorn, D. J. (1979b). Screening for resistance to a phatogenic specialization of Fusarium oxysporum f. sp. phaseoli the causal agent of bean yellows. Phytopathology, 69, 272-276. Recuperado de https://pdfs.semanticscholar.org/db0e/ 5ed4134f74dd330a966eeb81af41728980a2.pdf

Salgado, M. O. & Schwartz, H. F. (1993). Physiological specialization end effects of inoculum concentration on Fusarium oxysporum f. sp. phaseoli in common beans. Plant Disease, 79, 492-496. Recuperado de https://www.apsnet.org/publications/plantdisease/ backissues/Documents/1993Articles/PlantDisease77n05_492.PDF

Salgado, M. O.; Schwartz, H. F.; Brick, M. A. (1995). Inheritance of resistance to a Colorado race of Fusarium oxysporum f. sp. phaseoli in common beans. Plant Disease. 79, 279-281. Recuperado de https://www.apsnet.org/publications/plantdisease/backissues/ Documents/1995Articles/PlantDisease79n03_279.PDF

Santos, G. R., Costa, H., Pelúzio, J. M., Miranda, G. V. (1996). Transporte, transmissibilidade e patogenicidade da micoflora associada às sementes de feijão (Phaseolus vulgaris L.). Revista Ceres, 43(249), 621–627. Recuperado de http://www.ceres.ufv.br/ ojs/index.php/ceres/article/view/2365/375

Summerell, B. A., Laurence, M. H., Edward, C. Y., Leslie, J. F. (2010). Biogeography and phylogeograohy of Fusarium: a review. Fungal diversity, 44, 3-13. doi: 10.1007/s13225-010-0060-2

Taylor, A., Vagany, V., Jackson, A.C., Harrison, R.J., Rainoni, A. and Clarkson, J.P. (2016). Identification of pathogenicity-related genes in Fusarium oxysporum f.sp. cepae. Molecular Plant Pathology, 17, 1032–1047. doi: 10.1111/mpp.12346

Toledo-Souza, E.D.D.; Silveira, P.M.D.; Café‑Filho, A.C.; Lobo Júnior, M. (2012). Fusarium wilt incidence and common bean yield according to the preceding crop and the soil tillage system. Pesquisa Agropecuária Brasileira, 47, 1031-1037. doi: 10.1590/S0100-204X2012000800002

Wendland, A., Möller, P. A., Cortes, M. V. B., Lobo, M., Jr., Melo, L. C., Pereira, H. S., Costa, G. C., & Faria, L. C. (2012). Novas raças de Fusarium oxysporum f. sp. phaseoli identificadas via detecção específica por PCR. Summa Phytopathologica, 38. Recuperado de https://www.alice.cnptia.embrapa.br/handle/doc/935424

Woo, S. L., Zoina, A., Sorbo, G. D., Lorito, M., Nanni, B., Scala, F., Noviello, C. (1996). Characterization of Fusarium oxysporum f. sp. phaseoli by pathogenic races, VCGs, RFLPs and RAPD. Phytopathology, 86 (9), 966–973. doi: 10.1094/phyto-86-966

Wordell Filho, J. A.; De Freitas, M. B.; Stadnik, M. J.; Theodoro, G. F. (2013). Manejo de doenças na cultura do feijão. In: Wordell, J. A. F.; Chiaradia, L. A.; Balbinot, A. (Eds), Manejo fitossanitário na cultura do feijão (pp. 9-48), Florianopolis: DIOESC.

Xue, R. F., Wu, J., Zhu, Z., Wang, L., Wang, S. M., Blair, M. W. (2015). Differentially expressed genes in resistant and susceptible common bean (Phaseolus vulgaris L.) genotypes in response to Fusarium oxysporum f. sp. phaseoli. PLoS One, 10 (6), 1-20. doi: 10.1371/journal.pone.0127698

Published

29/08/2020

How to Cite

NEGREIROS, M. M. de .; BENEDETTI, A. R. .; CARVALHO, B. M. .; SILVA, A. G. da .; CARVALHO, M. C. da C. G. de; TORRES, J. P. . Reaction of commercial cultivars of common bean to fusarium oxysporum f. sp. phaseoli. Research, Society and Development, [S. l.], v. 9, n. 9, p. e531997506, 2020. DOI: 10.33448/rsd-v9i9.7506. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/7506. Acesso em: 13 nov. 2024.

Issue

Section

Agrarian and Biological Sciences