Applicability of saliva in the diagnosis of COVID-19: a review
DOI:
https://doi.org/10.33448/rsd-v9i9.7991Keywords:
Coronavirus; Saliva; Infection control; Infectious diseasesAbstract
In 2020, the World Health Organization (WHO) classified COVID-19 as a global pandemic. Since then, there is a need for new methods to facilitate the diagnosis and control of this disease. Currently, reverse transcription followed by real-time polymerase chain reaction (rRT-PCR) of respiratory samples obtained by swabs represents the gold standard in the qualitative detection of Sars-CoV-2 infection. However, this type of collection has several disadvantages, making saliva a potential tool for the diagnosis of COVID-19. Thus, the aim of this study is to evaluate, through a systematic review of current scientific literature, the applicability of saliva for the diagnosis of COVID-19 in comparison to current methods. A search was carried out in MEDLINE, SciELO, Scopus and Web of Science databases, using descriptors, strategies and pre-established criteria by two independent evaluators, followed by a manual search in the references of articles selected for full reading. The research strategies identified 476 studies and 1 study was added through manual search. After analysis, 200 articles were excluded because they were duplicated among results found in databases. With the completion of the screening process, 12 articles were included in this review. It was concluded that it is necessary to produce new studies in order to obtain even more reliable and effective data about the use of saliva in the diagnosis of COVID-19. However, studies have shown that this material can be an excellent alternative sample for the detection of SARS-CoV-2.
References
Azzi, L., Carcano, G., Gianfagna, F., Grossi, P., Gasperina, D. D., Genoni, A., ..., Baj, A. (2020). Saliva is a reliable tool to detect SARS-CoV-2. The Journal of infection, 81(1), e45–e50. https://doi.org/10.1016/j.jinf.2020.04.005
Brandtzaeg, P. (2013). Secretory immunity with special reference to the oral cavity. Journal of oral microbiology, 5(1), 20401. https://doi.org/10.3402/jom.v5i0.20401
Bulut, C., & Kato, Y. (2020). Epidemiology of COVID-19. Turkish journal of medical sciences, 50(SI-1), 563–570. https://doi.org/10.3906/sag-2004-172
Ceron, J. J., Lamy, E., Martinez-Subiela, S., Lopez-Jornet, P., Capela E Silva, F., Eckersall, P. D., & Tvarijonaviciute, A. (2020). Use of Saliva for Diagnosis and Monitoring the SARS-CoV-2: A General Perspective. Journal of clinical medicine, 9(5), 1491. https://doi.org/10.3390/jcm9051491
Chen, J. H., Yip, C. C., Poon, R. W., Chan, K. H., Cheng, V. C., Hung, I. F., Chan, J. F., Yuen, K. Y., & To, K. K. (2020). Evaluating the use of posterior oropharyngeal saliva in a point-of-care assay for the detection of SARS-CoV-2. Emerging microbes & infections, 9(1), 1356–1359. https://doi.org/10.1080/22221751.2020.1775133
Faustini, S. E., Jossi, S. E., Perez-Toledo, M., Shields, A., Allen, J. D., Watanabe, Y., … & Goodall, M. (2020). Detection of antibodies to the SARS-CoV-2 spike glycoprotein in both serum and saliva enhances detection of infection. medRxiv: the preprint server for health sciences. https://doi.org/10.1101/2020.06.16.20133025.
Golatowski, C., Salazar, M. G., Dhople, V. M., Hammer, E., Kocher, T., Jehmlich, N., & Völker, U. (2013). Comparative evaluation of saliva collection methods for proteome analysis. Clinica chimica acta; international journal of clinical chemistry, 419, 42–46. https://doi.org/10.1016/j.cca.2013.01.013
Huang, C., Wang, Y., Li, X., Ren, L., Zhao, J., Hu, Y., …, Cheng, Z. (2020). Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. The lancet, 395(10223), 497-506. https://doi.org/10.1016/S0140-6736(20)30183-5
Hung, D. L., Li, X., Chiu, K. H., Yip, C. C., To, K. K., Chan, J. F. … & Yuen, K. Y. (2020). Early-Morning vs Spot Posterior Oropharyngeal Saliva for Diagnosis of SARS-CoV-2 Infection: Implication of Timing of Specimen Collection for Community-Wide Screening. Open forum infectious diseases, 7(6), ofaa210. https://doi.org/10.1093/ofid/ofaa210
Martina, E., Campanati, A., Diotallevi, F., & Offidani, A. (2020). Saliva and Oral Diseases. Journal of clinical medicine, 9(2), 466. https://doi.org/10.3390/jcm9020466
Mesa, J. F. C., & Vitón Castillo, A. A. (in press). Real-time PCR-based SARS-CoV-2 detection. In Real-time PCR-based SARS-CoV-2 detection. doi: https://doi.org/10.1590/SciELOPreprints.707
Ng, K., Poon, B. H., Kiat Puar, T. H., Shan Quah, J. L., Loh, W. J., Wong, Y. J., Tan, T. Y., & Raghuram, J. (2020). COVID-19 and the Risk to Health Care Workers: A Case Report. Annals of internal medicine, 172(11), 766–767. https://doi.org/10.7326/L20-0175
Pasomsub, E., Watcharananan, S. P., Boonyawat, K., Janchompoo, P., Wongtabtim, G., Suksuwan, W., Sungkanuparph, S., & Phuphuakrat, A. (2020). Saliva sample as a non-invasive specimen for the diagnosis of coronavirus disease 2019: a cross-sectional study. Clinical microbiology and infection: the official publication of the European Society of Clinical Microbiology and Infectious Diseases, S1198-743X (20)30278-0. https://doi.org/10.1016/j.cmi.2020.05.001
Randad, P. R., Pisanic, N., Kruczynski, K., Manabe, Y. C., Thomas, D., Pekosz, A. …, & Heaney, C. D. (2020). COVID-19 serology at population scale: SARS-CoV-2-specific antibody responses in saliva. medRxiv : the preprint server for health sciences, https://doi.org/10.1101/2020.05.24.20112300
Sri Santosh, T., Parmar, R., Anand, H., Srikanth, K., & Saritha, M. (2020). A Review of Salivary Diagnostics and Its Potential Implication in Detection of Covid-19. Cureus, 12(4), e7708. https://doi.org/10.7759/cureus.7708
Tajima, Y., Suda, Y., & Yano, K. (2020). A case report of SARS-CoV-2 confirmed in saliva specimens up to 37 days after onset: Proposal of saliva specimens for COVID-19 diagnosis and virus monitoring. Journal of infection and chemotherapy: official journal of the Japan Society of Chemotherapy, S1341-321X (20) 30202-6. Advance online publication. https://doi.org/10.1016/j.jiac.2020.06.011
Taylor, J. J., & Preshaw, P. M. (2016). Gingival crevicular fluid and saliva. Periodontology 2000, 70(1), 7–10. https://doi.org/10.1111/prd.12118
To, K. K., Tsang, O. T., Leung, W. S., Tam, A. R., Wu, T. C., Lung, D. C., …, Yuen, K. Y. (a) (2020). Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study. The Lancet. Infectious diseases, 20(5), 565–574. https://doi.org/10.1016/S1473-3099(20)30196-1
To, K. K., Tsang, O. T., Yip, C. C., Chan, K. H., Wu, T. C., Chan, J. M., … Yuen, K. Y. (b) (2020). Consistent Detection of 2019 Novel Coronavirus in Saliva. Clinical infectious diseases: an official publication of the Infectious Diseases Society of America, 71(15), 841–843. https://doi.org/10.1093/cid/ciaa149
WHO. (2020) (a). Novel Coronavirus – China. Retrieved from: https://www.who.int/csr/don/12-january-2020-novel-coronavirus-china/en/
WHO. (2020) (b). Coronavirus disease (COVID-19) pandemic. Retrieved from: https://www.who.int/emergencies/diseases/novel-coronavirus-2019
Woźniak, M., Paluszkiewicz, C., & Kwiatek, W. M. (2019). Saliva as a non-invasive material for early diagnosis. Acta biochimica Polonica, 66(4), 383–388. https://doi.org/10.18388/abp.2019_2762
Wyllie, A. L., Fournier, J., Casanovas-Massana, A., Campbell, M., Tokuyama, M., Vijayakumar, P., ..., Petrone, M. E. (2020). Saliva is more sensitive for SARS-CoV-2 detection in COVID-19 patients than nasopharyngeal swabs. Medrxiv. the preprint server for health sciences. doi: https://doi.org/10.1101/2020.04.16.20067835
Yang, X., Yu, Y., Xu, J., Shu, H., Xia, J., Liu, H., ... , Shang, Y. (2020) Clinical course and outcomes of critically ill patients with SARS-CoV-2 pneumonia in Wuhan, China: a single-centered, retrospective, observational study. The Lancet. Respiratory medicine, 8(5), 475–481. https://doi.org/10.1016/S2213-2600(20)30079-5
Yoon, J. G., Yoon, J., Song, J. Y., Yoon, S. Y., Lim, C. S., Seong, H., Noh, J. Y., Cheong, H. J., & Kim, W. J. (2020). Clinical Significance of a High SARS-CoV-2 Viral Load in the Saliva. Journal of Korean medical science, 35(20), e195. https://doi.org/10.3346/jkms.2020.35.e195
Zheng, S., Fan, J., Yu, F., Feng, B., Lou, B., Zou, Q. … & Liang, T. (2020). Viral load dynamics and disease severity in patients infected with SARS-CoV-2 in Zhejiang province, China, January-March 2020: retrospective cohort study. BMJ (Clinical research ed.), 369, m1443. https://doi.org/10.1136/bmj.m1443
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Copyright (c) 2020 Éverson de Andrade Lemos; Gustavo de Amorim Barbosa Cabral; José de Alencar Fernandes Neto; Maria Helena Chaves de Vasconcelos Catão
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