Drying and physiological quality of palheteira seeds (Clitoria fairchildiana R.A. Howard)
Keywords:Desiccation; Storage; Vigor; Seed moisture content.
Knowledge about seed storage behavior is important to preserve plant species. Clitoria fairchildiana R.A.Howard is an endemic species with medicinal properties, it is used in the recovery of degraded land. The objective of this study is to evaluate the effect of drying on the physiological quality of C. fairchildiana seeds. Seeds were oven dried along with silica gel for 18 days and in intervals of 2 or 3 days, samples were removed to assess the seed moisture content. This was done in an oven at 105±3 oC for 24h, using four replicates with 10 seeds each. After undergoing the above-mentioned treatment, a sample of seeds was stored in the freezer (-18 ºC) for 3 months. Sowing was carried out in plastic trays in a sand and sawdust mixture (1:1), previously sterilized in hot water (100 oC) for two hours. Seeds were left to germinate in a laboratory with no temperature and relative humidity control. Germination tests were performed over 14 days with daily counts of the number of emerged seedlings. The percentage of seed germination, abnormal seedlings and dead seeds were obtained 14 days after sowing. The experimental design was completely randomized with four replications of 25 seeds. The reduction of seed moisture content from 18.5% to 5.6% affected physiological seed quality causing a reduction in the germination percentage and an increase in dead seeds and abnormal seedling percentage. C. fairchildiana seeds are classified as intermediate and they can be desiccated up to 8.6% with no reduction in physiology quality.
Alves, M. M., Alves, E. U., & Santos-Moura, S. S. (2015). Physiological quality seeds of Clitoria fairchildiana R.A.Howard during storage. Bioscience Journal, 31 (3), 767-774, 2015. DOI: https://doi.org/10.14393/BJ-v31n3a2015-26085
Barros, H. S. D., Cruz, E. D., & Pereira, A. G. (2019). Classificação fisiológica de sementes de maçaranduba quanto a tolerância à dessecação e ao armazenamento. Revista de Ciências Agrárias, 62. DOI: http://dx.doi.org/10.22491/rca.2019.2949
Baskin, J. M., & Baskin, C.C. (2004) A Classification System for Seed Dormancy. Seed Science Research, 14, 1-16. DOI: http://dx.doi.org/10.1079/SSR2003150
Brasil. Ministério da Agricultura, Pecuária e Abastecimento. (2009). Regras para Análise de Sementes. Brasília: Mapa/ACS.
BFG. (2015). Growing knowledge: an overview of seed plant diversity in Brazil. Rodriguésia, 66 (4), 1085-1113. DOI: 10.1590/2175-7860201566411
Costa, M.C.D., Cooper, K., Hilhorst, H.W.M., & Farrant, J.M. (2017) Orthodox Seeds and Resurrection Plants: Two of a Kind? Plant Physiology, 175, 589–599. DOI: https://doi.org/10.1104/pp.17.00760
Cruz, E. D., & Pereira, A. G. (2019). Germinação de sementes de espécies amazônicas: palheteira (Clitoria fairchildiana R.A.Howard). Belém, PA: Embrapa Amazônia Oriental. (Technical Bulletin, 314).
Cruz, E. D. & Cicero, S. M. (2008). Sensitivity of seed to desiccation in cupuassu (Theobroma grandiflorum (Willd. ex Spreng.) K. Schum. – Sterculiaceae. Scientia Agricola, 65 (5), 557-560. DOI: https://doi.org/10.1590/S0103-90162008000500017
Dantzger, M., Vasconcelos, I. M., Scorsato, V., Aparicio, R., Marangoni, S., & Macedo, M. L. R. (2015). Bowman–Birk proteinase inhibitor from Clitoria fairchildiana seeds: Isolation, biochemical properties and insecticidal potential. Phytochemistry, 118, 224-235. DOI: https://doi.org/10.1016/j.phytochem.2015.08.013
Ellis, R. H., Hong, T. D., & Roberts, H. (1990). An intermediate category of seed storage behaviour? I. Coffee. Journal of Experimental of Botany, 41 (230), 1167-1174. DOI: https://doi.org/10.1093/jxb/41.9.1167
Fantz, P. R. (1990). Clitoria (Leguminosae) antillarum. Moscosoa, 6, 152-166.
Flora do Brasil 2020 em construção. (2020). Clitoria. Retrived February 18, 2020, from http://floradobrasil.jbrj.gov.br/reflora/floradobrasil/FB29540.
Hong, T. D. & Ellis, R. H. (1996). A protocol to determine seed storage behavior. Rome: International Plant Genetic Resources Institute. (Technical Bulletin, 1).
Hong, T. D., Linington, S., & Ellis, R. H. (1998). Compendium of information on seed storage behavior. Kew: Basingstore Press.
Kleinwächter, M., Radwan, A., Hara, M., & Selmar, D. (2014). Dehydrin expression in seeds: an issue of maturation drying. Frontiers in Plant Science, 5 (402). DOI: https://doi.org/10.3389/fpls.2014.00402
Lorenzi, H. (2009) Árvores brasileiras: manual de identificação de e cultivo de plantas arbóreas nativas do Brasil. (3 ed). Nova Odessa: Instituto Plantarum.
Magistrali, P. R., José, A. C., Faria, J. M. R., & Gasparin, E. (2013). Physiological behavior of Genipa americana L. seeds regarding the capacity for desiccation and storage tolerance. Journal of Seed Science, 35 (4), 495-500. DOI: https://doi.org/10.1590/S2317-15372013000400011
Maguire, J. D. (1962). Speed of germination-aid in relation evaluation for seedling emergence vigor. Crop Science, 2 (2), 176-177.
Mayrinck, R. C., Vaz, T. A. A., & Davide, A. C. (2016) Classificação fisiológica de sementes florestais quanto à tolerância à dessecação e ao comportamento no armazenamento. Cerne, 22 (1), 85-92. DOI: https://doi.org/10.1590/01047760201622012064.
Pelissari, F., José, A. C., Fontes, M. A. L., Matos, A. C. B., Pereira, W. V. S., & Faria, J. M. R. (2018). A probabilistic model for tropical tree seed desiccation tolerance and storage classification. New Forests, 49, 143–158. DOI: https://doi.org/10.1007/s11056-017-9610-8
Rando, J. G., & Souza, V.C. (2015). Clitoria in Lista de Espécies da Flora do Brasil. Retrieved November 12, 2019, from http://floradobrasil.jbrj.gov.br/jabot/floradobrasil/FB29540
Roberts, E. H. (1973). Predicting the storage life of seeds. Seed Science and Seed Technology, 1 (3), 499-514.
Sano, N.; Rajjou, L., North, H. M., Debeaujon, I., Marion-Poll, A., & Seo, M. (2016). Staying Alive: Molecular Aspects of Seed Longevity. Plant & Cell Physiology, 57 (4), 660–674. DOI: 10.1093/pcp/pcv186
Santos, R. A. F. (2014). Estudo fitoquímico e avaliação biológica dos flavonoides isolados de Clitoria fairchildiana R. A. Howard. (Unpublished doctoral thesis). Universidade Federal da Bahia/UFBA, Salvador.
Smaniotto, T. A. S., Resende, O., Marçal, K. A. F., Oliveira, D. E. C., & Simon, G. A. (2014). Qualidade fisiológica das sementes de soja armazenadas em diferentes condições. Revista Brasileira de Engenharia Agrícola e Ambiental, 18 (4), 446–453. DOI: https://doi.org/10.1590/S1415-43662014000400013
STATSOFT. (2016). Statistica for windows. (13.2. ed). Tulsa: StatSoft. (General conventions and statistics).
ZAR, J. H. (1996). Biostatistical analysis. Upper Saddle River: Prentice Hall.
How to Cite
Copyright (c) 2020 Érica Coutinho David; Bressa Karolina Dias Cardoso; Josiene Amanda dos Santos Viana; Eniel David Cruz
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.