Essential Oils of Garlic and Oregano Incorporated in Cellulose Acetate Films: Antimicrobial Activity and Physical Properties

Authors

DOI:

https://doi.org/10.33448/rsd-v9i10.8304

Keywords:

Listeria monocytogenes; Optical properties; Elastic modulus; Active compounds.

Abstract

Polymers of natural origin and their derivatives are currently used as biomaterials because they are easily available and their properties can be tailored to meet specific requirements. The essential oils are widely used as antimicrobials.  The objective of this study was to evaluate the in-vitro antimicrobial efficiency of cellulose acetate (CA) films incorporated with the essential oils of garlic (GR) and oregano (OR) on the microorganisms Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, Salmonella choleraesuis and Pseudomonas aeruginosa and characterize the films as to their mechanical, optical and structural properties. Four treatments were evaluated, Control, Film 1 (50 ml OR.100 g-1 CA) Film 2 (50 ml OR + 30 ml GR.100 g-1 CA) and Film 3 (50 ml OR + 50 ml GR.100 g-1 CA). The concentration of oils influenced the mechanical parameters of maximum load, relative deformation at maximum load and elastic modulus, resulting in weaker, less rigid and more flexible films. There was an increase in L* and b* in films incorporated with garlic and oregano essential oil. The films incorporated with a mixture of oregano and garlic essential oils exhibited inhibition against all organisms tested.

References

Abdel-Naby, A. S., & Aboubshait, S. A. (2013). Cellulose acetate blends with acrylonitrile/N-phenyl maleimide copolymers morphological and thermal properties. Journal of Thermal Analysis and Calorimetry, 114(3), 1279-1286. doi:10.1007/s10973-013-3153-9

Appendini, P., & Hotchkiss, J. H. (2002). Review of antimicrobial food packaging. Innovative Food Science & Emerging Technologies, 3(2), 113-126. doi:https://doi.org/10.1016/S1466-8564(02)00012-7

Assis, R. Q., Rios, P. D. A., Rios, A. d. O., & Olivera, F. C. (2020). Biodegradable packaging of cellulose acetate incorporated with norbixin, lycopene or zeaxanthin. Industrial Crops and Products, 147, 112212. doi:https://doi.org/10.1016/j.indcrop.2020.112212

ASTM. (2002). Standard test method for tensile properties of thin plastic sheeting (Vol. 14, pp. 161-170): ASTM International (EUA).

ASTM, A. S. (2000). D618, Standard Practice for Conditioning Plastics for Testing, ASTM International, West Coshoshocken, PA. Retrieved from

Ataei, S., Azari, P., Hassan, A., Pingguan-Murphy, B., Yahya, R., & Muhamad, F. (2020). Essential Oils-Loaded Electrospun Biopolymers: A Future Perspective for Active Food Packaging. Advances in Polymer Technology, 2020.

Biliaderis, C. G., Lazaridou, A., & Arvanitoyannis, I. (1999). Glass transition and physical properties of polyol-plasticised pullulan–starch blends at low moisture. Carbohydrate polymers, 40(1), 29-47. doi:https://doi.org/10.1016/S0144-8617(99)00026-0

Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods—a review. International Journal of Food Microbiology, 94(3), 223-253. doi:https://doi.org/10.1016/j.ijfoodmicro.2004.03.022

Carvalho, D. d. M., Takeuchi, K. P., Geraldine, R. M., Moura, C. J. d., & Silveira, M. F. A. (2017). Filme ativo de acetato de celulose incorporado com nanosuspensão de curcumina. Polímeros, 27(SPE), 70-76.

Carvalho, R. A., de Oliveira, A. C. S., Santos, T. A., Dias, M. V., Yoshida, M. I., & Borges, S. V. (2019). WPI and Cellulose Nanofibres Bio-nanocomposites: Effect of Thyme Essential Oil on the Morphological, Mechanical, Barrier and Optical Properties. Journal of Polymers and the Environment, 1-11.

Cerqueira, T. S., Jacomino, A. P., Sasaki, F. F., & Alleoni, A. (2011). Protein and chitosan coatings on guavas. Bragantia, 70(1), 216-221.

Cerqueira, T. S., Jacomino, A. P., Sasaki, F. F., & Alleoni, A. C. C. (2011). Recobrimento de goiabas com filmes proteicos e de quitosana. Bragantia [online]. 2011, vol. 70, n. 1: SciELO Brasil.

Chuai, C. Z., & Zhang, Z. (2014). Effect of polyethylene glycol (PEG) on molten plasticized cellulose acetate (CA). Paper presented at the Advanced Materials Research.

Coma, V., Sebti, I., Pardon, P., Deschamps, A., & Pichavant, F. H. (2001). Antimicrobial Edible Packaging Based on Cellulosic Ethers, Fatty Acids, and Nisin Incorporation To Inhibit Listeria innocua and Staphylococcus aureus. Journal of Food Protection, 64(4), 470-475. doi:10.4315/0362-028x-64.4.470

Cosentino, S., Tuberoso, C. I. G., Pisano, B., Satta, M., Mascia, V., Arzedi, E., & Palmas, F. (1999). In-vitro antimicrobial activity and chemical composition of Sardinian Thymus essential oils. Letters in Applied Microbiology, 29(2), 130-135. doi:10.1046/j.1472-765X.1999.00605.x

Dannenberg, G. d. S., Funck, G. D., Cruxen, C. E. d. S., Marques, J. d. L., Silva, W. P. d., & Fiorentini, Â. M. (2017). Essential oil from pink pepper as an antimicrobial component in cellulose acetate film: Potential for application as active packaging for sliced cheese. LWT - Food Science and Technology, 81, 314-318. doi:https://doi.org/10.1016/j.lwt.2017.04.002

Davidson, P. (1989). Methods for testing the efficacy of food antimicrobials. Food technology, 43, 148-155.

de Almeida, D. S., Duarte, E. H., Hashimoto, E. M., Turbiani, F. R. B., Muniz, E. C., de Souza, P. R., . . . Martins, L. D. (2020). Development and characterization of electrospun cellulose acetate nanofibers modified by cationic surfactant. Polymer Testing, 81, 106206. doi:https://doi.org/10.1016/j.polymertesting.2019.106206

de Castro e Silva, P., de Oliveira, A. C. S., Pereira, L. A. S., Valquíria, M., Carvalho, G. R., Miranda, K. W. E., . . . Oliveira, J. E. (2019). Development of bionanocomposites of pectin and nanoemulsions of carnauba wax and neem oil pectin/carnauba wax/neem oil composites. Polymer Composites, n/a(n/a). doi:10.1002/pc.25416

de Castro e Silva, P., Pereira, L. A. S., Lago, A. M. T., Valquíria, M., de Rezende, É. M., Carvalho, G. R., . . . Marconcini, J. M. (2019). Physical-Mechanical and Antifungal Properties of Pectin Nanocomposites / Neem Oil Nanoemulsion for Seed Coating. Food Biophysics, 14(4), 456-466. doi:10.1007/s11483-019-09592-0

de Oliveira, A. C. S., Ugucioni, J. C., da Rocha, R. A., & Borges, S. V. (2018). Development of whey protein isolate/polyaniline smart packaging: Morphological, structural, thermal, and electrical properties. Journal of Applied Polymer Science, 47316. doi:10.1002/app.47316

de Oliveira, A. C. S., Ugucioni, J. C., da Rocha, R. A., Santos, T. A., & Borges, S. V. (2019). Chitosan/Polyaniline Conductive Blends for Developing Packaging: Electrical, Morphological, Structural and Thermal Properties. Journal of Polymers and the Environment, 27(10), 2250-2258. doi:10.1007/s10924-019-01519-7

Devlieghere, F., Vermeulen, A., & Debevere, J. (2004). Chitosan: antimicrobial activity, interactions with food components and applicability as a coating on fruit and vegetables. Food Microbiology, 21(6), 703-714. doi:https://doi.org/10.1016/j.fm.2004.02.008

Du, W.-X., Olsen, C. W., Avena-Bustillos, R. J., McHugh, T. H., Levin, C. E., & Friedman, M. (2009). Effects of Allspice, Cinnamon, and Clove Bud Essential Oils in Edible Apple Films on Physical Properties and Antimicrobial Activities. Journal of Food Science, 74(7), M372-M378. doi:10.1111/j.1750-3841.2009.01282.x

Felgueiras, H. P., Teixeira, M. A., Tavares, T. D., & Amorim, M. T. P. (2020). New method to produce poly(vinyl alcohol)/cellulose acetate films with improved antibacterial action. Materials Today: Proceedings. doi:https://doi.org/10.1016/j.matpr.2019.12.100

Ferreira, D. F. (2014). Sisvar: a Guide for its Bootstrap procedures in multiple comparisons. Ciência e agrotecnologia, 38(2), 109-112.

Ghasemlou, M., Aliheidari, N., Fahmi, R., Shojaee-Aliabadi, S., Keshavarz, B., Cran, M. J., & Khaksar, R. (2013). Physical, mechanical and barrier properties of corn starch films incorporated with plant essential oils. Carbohydrate polymers, 98(1), 1117-1126. doi:https://doi.org/10.1016/j.carbpol.2013.07.026

Giménez, B., Gómez-Estaca, J., Alemán, A., Gómez-Guillén, M. C., & Montero, M. P. (2009). Physico-chemical and film forming properties of giant squid (Dosidicus gigas) gelatin. Food Hydrocolloids, 23(3), 585-592. doi:https://doi.org/10.1016/j.foodhyd.2008.07.003

Han Lyn, F., & Nur Hanani, Z. A. (2020). Effect of Lemongrass (Cymbopogon citratus) Essential Oil on the Properties of Chitosan Films for Active Packaging. Journal of Packaging Technology and Research. doi:10.1007/s41783-019-00081-w

Harpaz, S., Glatman, L., Drabkin, V., & Gelman, A. (2003). Effects of Herbal Essential Oils Used To Extend the Shelf Life of Freshwater-Reared Asian Sea Bass Fish (Lates calcarifer). Journal of Food Protection, 66(3), 410-417. doi:10.4315/0362-028x-66.3.410

Kırkpınar, F., Ünlü, H. B., & Özdemir, G. (2011). Effects of oregano and garlic essential oils on performance, carcase, organ and blood characteristics and intestinal microflora of broilers. Livestock Science, 137(1), 219-225. doi:https://doi.org/10.1016/j.livsci.2010.11.010

Molina-Hernández, J. B., Castro, A. E., Martinez-Correa, H. A., & Andrade-Mahecha, M. M. (2020). Edible coating based on achira starch containing garlic/oregano oils to extend the shelf life of double cream cheese. Revista Facultad Nacional de Agronomia Medellin, 73(1), 9099-9108.

Moreira Gonçalves, S., Gomes Motta, J. F., Ribeiro-Santos, R., Hidalgo Chávez, D. W., & Ramos de Melo, N. (2020). Functional and antimicrobial properties of cellulose acetate films incorporated with sweet fennel essential oil and plasticizers. Current Research in Food Science, 3, 1-8. doi:https://doi.org/10.1016/j.crfs.2020.01.001

Ouattara, B., Simard, R. E., Piette, G., Bégin, A., & Holley, R. A. (2000). Diffusion of Acetic and Propionic Acids from Chitosan-based Antimicrobial Packaging Films. Journal of Food Science, 65(5), 768-773. doi:10.1111/j.1365-2621.2000.tb13584.x

Pelissari, F. M., Grossmann, M. V., Yamashita, F., & Pineda, E. A. G. (2009). Antimicrobial, mechanical, and barrier properties of cassava starch− chitosan films incorporated with oregano essential oil. Journal of agricultural and food chemistry, 57(16), 7499-7504.

Pereda, M., Aranguren, M. I., & Marcovich, N. E. (2010). Caseinate films modified with tung oil. Food Hydrocolloids, 24(8), 800-808. doi:https://doi.org/10.1016/j.foodhyd.2010.04.007

Pintore, G., Usai, M., Bradesi, P., Juliano, C., Boatto, G., Tomi, F., . . . Casanova, J. (2002). Chemical composition and antimicrobial activity of Rosmarinus officinalis L. oils from Sardinia and Corsica. Flavour and Fragrance Journal, 17(1), 15-19. doi:10.1002/ffj.1022

Pola, C. C., Medeiros, E. A. A., Pereira, O. L., Souza, V. G. L., Otoni, C. G., Camilloto, G. P., & Soares, N. F. F. (2016). Cellulose acetate active films incorporated with oregano (Origanum vulgare) essential oil and organophilic montmorillonite clay control the growth of phytopathogenic fungi. Food Packaging and Shelf Life, 9, 69-78. doi:https://doi.org/10.1016/j.fpsl.2016.07.001

Porta, R., Mariniello, L., Di Pierro, P., Sorrentino, A., & Giosafatto, C. V. L. (2011). Transglutaminase crosslinked pectin-and chitosan-based edible films: A review. Critical reviews in food science and nutrition, 51(3), 223-238.

Ramos, E., & Gomide, L. (2017). Avaliação da Qualidade de Carnes: Fundamentos e Metodologias (ed.). Viçosa: Editora UFV.

Rooney, M. (1995). Overview of active food packaging Active food packaging (pp. 1-37): Springer.

Seydim, A. C., & Sarikus, G. (2006). Antimicrobial activity of whey protein based edible films incorporated with oregano, rosemary and garlic essential oils. Food Research International, 39(5), 639-644. doi:https://doi.org/10.1016/j.foodres.2006.01.013

Shojaee-Aliabadi, S., Hosseini, H., Mohammadifar, M. A., Mohammadi, A., Ghasemlou, M., Ojagh, S. M., . . . Khaksar, R. (2013). Characterization of antioxidant-antimicrobial κ-carrageenan films containing Satureja hortensis essential oil. International journal of biological macromolecules, 52, 116-124. doi:https://doi.org/10.1016/j.ijbiomac.2012.08.026

Silva, J. F. M. d., Prado, G., Madeira, J. E. G. C., Oliveira, M. S., Faraco, A. A. G., Malta, C. M., . . . Pimenta, R. S. (2015). Utilização de filme de quitosana para o controle de aflatoxinas em amendoim. Bragantia, 74(4), 467-475.

Silveira, M. F. A., Soares, N. F. F., Geraldine, R. M., Andrade, N. J., Botrel, D. A., & Gonçalves, M. P. J. (2007). Active film incorporated with sorbic acid on pastry dough conservation. Food Control, 18(9), 1063-1067. doi:https://doi.org/10.1016/j.foodcont.2006.07.004

Vermeiren, L., Devlieghere, F., van Beest, M., de Kruijf, N., & Debevere, J. (1999). Developments in the active packaging of foods. Trends in food science & technology, 10(3), 77-86. doi:https://doi.org/10.1016/S0924-2244(99)00032-1

Wang, D., Yue, Y., Wang, Q., Cheng, W., & Han, G. (2020). Preparation of cellulose acetate-polyacrylonitrile composite nanofibers by multi-fluid mixing electrospinning method: Morphology, wettability, and mechanical properties. Applied Surface Science, 510, 145462. doi:https://doi.org/10.1016/j.apsusc.2020.145462

Yang, L., & Paulson, A. T. (2000). Effects of lipids on mechanical and moisture barrier properties of edible gellan film. Food Research International, 33(7), 571-578. doi:https://doi.org/10.1016/S0963-9969(00)00093-4

Zivanovic, S., Chi, S., & Draughon, A. F. (2005). Antimicrobial Activity of Chitosan Films Enriched with Essential Oils. Journal of Food Science, 70(1), M45-M51. doi:10.1111/j.1365-2621.2005.tb09045.x

Downloads

Published

17/09/2020

How to Cite

COSTA, A. L. R. .; OLIVEIRA, A. C. S. de .; AZEVEDO, V. M. .; MEDEIROS, E. A. A. .; SOARES, N. de F. F. .; BORGES, S. V. . Essential Oils of Garlic and Oregano Incorporated in Cellulose Acetate Films: Antimicrobial Activity and Physical Properties. Research, Society and Development, [S. l.], v. 9, n. 10, p. e329108304, 2020. DOI: 10.33448/rsd-v9i10.8304. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/8304. Acesso em: 24 dec. 2024.

Issue

Section

Engineerings