Organic management vs. conventional management influence the antimicrobial activity of essential oils of Origanum vulgare L
Keywords:Vermicomposting; S. aureus; E. coli; C. albicans; Agroecology; Natural antimicrobial.
The objective this stud was to evaluate the antimicrobial activity of essential oils (organic vs. conventional) of Origanum vulgare L. in the action against Candida albicans, Escherichia coli and Staphylococcus aureus. The inoculation of rhizobacteria (environmental (ME) and Bacillus subtilis) potentiated the antimicrobial action. The essential oils of Traditional (organic) cultivation presented antibacterial action and antifungal, while the essential oil of conventional cultivation (NPK) showed no any antibacterial or antifungal activity. It was concluded that the formulation of vermicomposting and the use of rhizobacteria are potential technologies and tools for family farmers and traditional community in the cultivation of orégano.
Ayyobi, H., Olfati, J. A., Peyvast, G. A. (2014). The effects of cow manure vermicompost and municipal solid waste compost on peppermint (Mentha piperita L.) in Torbat-e-Jam and Rasht regions of Iran. International Journal of Recycling of Organic Waste in Agriculture, 3(4), 147–153.
Bakkali, F., et al. Biological effects of essential oil: a review (2008). Food and Chemical Toxicology, 46 (2), 446- 75,.
Basak, B. B., et al. (2020). Organic nutrient management through manures, microbes and biodynamic preparation improves yield and quality of Kalmegh (Andrograghis paniculata), and soil properties. Journal of Plant Nutrition, 43(4), 548–562.
Bhat, V., Sharma, S. M., Shetty, V., Shastry, C. S., Rao, C. V., Shenoy, S., & Balaji, S. (2018). Characterization of herbal antifungal agent, Origanum vulgare against Oral Candida spp. isolated from patients with Candida-associated denture stomatitis: an in vitro study. Contemporary clinical dentistry, 9(Suppl 1), S3.
Bhattacharyya, P. N., JHA, D. K. (2012). Plant growth-promoting rhizobacteria (PGPR): Emergence in agriculture. World Journal of Microbiology and Biotechnology, 28(4), 1327–1350.
Brondani, L. P., da Silva Neto, T. A., Freitag, R. A., & Lund, R. G. (2018). Evaluation of anti-enzyme properties of Origanum vulgare essential oil against oral Candida albicans. Journal de mycologie medicale, 28 (1), 94-100.
Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods - a review. International Journal of Food Microbiology, 94 (3), 223-53.
Burt, Sara A., et al. (2005). Increase in activity of essential oil components carvacrol and thymol against Escherichia coli O157: H7 by addition of food stabilizers. Journal of food protection, 68 (5), 919-926.
Busatta, C., Mossi, A. J., Rodrigues, M. R. A., Cansian, R. L., & Oliveira, J. V. D. (2007). Evaluation of Origanum vulgare essential oil as antimicrobial agent in sausage. Brazilian Journal of Microbiology, 38 (4), 610-616.
Camiletti, B. X., Asensio, C. M., Gadban, L. C., et al. (2016). Essential oils and their combinations with iprodione fungicide as potential antifungal agentes against withe rot (Sclerotium cepivorum Berk) in garlic (Allium sativum L.) crops. Ind. Crops Prod, (85) 117–124.
Canellas, L. P., Olivares, F. L., Aguiar, N. O., Jones, D. L., Nebbioso, A., Mazzei, P., & Piccolo, A. (2015). Humic and fulvic acids as biostimulants in horticulture. Scientia Horticulturae, (196), 15-27.
Carson, C. F., Mee, B., Riley, T. V. (2002) Mechanism of action of Melaleuca alternifolia (tea tree) oil on Staphylococcus aureus determined by time-kill, lysis, leakage, and salt tolerance assays and electron microscopy. Antimicrob Agents Chemother 46 (6),1914–1920.
Chami, N., Chami, F., Bennis, S., Trouillas, J., & Remmal, A. (2004). Antifungal treatment with carvacrol and eugenol of oral candidiasis in immunosuppressed rats. Brazilian Journal of Infectious Diseases, 8 (3), 217-226,
Chatterjee R, Bandyopadhyay S, Jana J. C. (, 2014). Evaluation of vegetable wastes recycled for Vermicomposting and its response on yield and quality of carrot (Daucus carota L.). Int J Recycl Org Waste Agric, (3) 60–67.
Chavarria, D. N. (2018). Response of soil microbial communities to agroecological versus conventional systems of extensive agriculture. Agriculture, Ecosystems and Environment, (264), 1-8.
Cleff, M. B., Meinerz, A. R., et al. (2010). In vitro activity of Origanum vulgare essential oil against Candida species. Brazilian Journal of Microbiology, 41(1), 116-123.
Clinical and laboratory standards institute (CLSI). (2015). Methods for Antimicrobial Susceptibility Testing of Aerobic Bacteria. Approved Standard, (10th ed.), M07-A10. Wayne, PA, USA: CLSI, (35), n. 2.
Clinical and laboratory standards institute CLSI). (2008). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts. Approved Standard, (3rd ed.), M27-A3. Wayne, PA, USA: CLSI, 28 (14).1- 13.
Coutinho A. M. Notebook of efficient microorganisms (EM). Practical instructions on the ecological and social use of EM, 2011.
Cui, H., Zhang, C., Li, C., & Lin, L. (2019). Antibacterial mechanism of oregano essential oil. Industrial Crops and Products, 139, 111498.
Da Silva Santos, C. H., Piccoli, R. H., & Tebaldi, V. M. R. (2017). Antimicrobial activity of essential oils and compounds isolated against pathogens of clinical and food origin. Rev Inst Adolfo Lutz, 76, 1719.
Dangour A, Lock K, Hayter A, Aikenhead A, Allen E, Uauy R. Nutrition-related health effects of organic foods: a systematic review. Am J Clin Nutr. 92, 203–210, 2010.
Dhifi, W., Bellili, S., Jazi, S., Bahloul, N., Mnif, W. (2016). Essential Oils’ Chemical Characterization and Investigation of Some Biological Activities: A Critical Review. Medicines, 3 (4), 25.
Dos Santos, R., Jessica B., et al. (2017). Effects of oregano essential oil and carvacrol on biofilms of Staphylococcus aureus from food-contact surfaces. Food Control, 73 (B), 1237-1246.
Dos Santos, R., Jessica B., et al. (2018). Efficacy of using oregano essential oil and carvacrol to remove young and mature Staphylococcus aureus biofilms on food-contact surfaces of stainless steel. LWT, 93, 293-299.
Ebani, V. V., Bertelloni, F., Najar, B., Nardoni, S., Pistelli, L., & Mancianti, F. (2020). Antimicrobial Activity of Essential Oils against Staphylococcus and Malassezia Strains Isolated from Canine Dermatitis. Microorganisms, 8 (2), 252.
Esmaielpour, B., et al. (2017). Effect of Vermicompost and Spent Mushroom Compost on the Nutrient and Essential Oil Composition of Basil (Ocimum basilicum L.). Journal of Essential Oil-Bearing Plants, 20 (5), 1283–1292.
Fikry, S., Khalil, N., Salama, O. (2019). Chemical profiling, biostatic and biocidal dynamics of Origanum vulgare L. essential oil. AMB Express, 9(41), 1 -10.
Ganjali, A., Kaykhaii, M. (2017). Investigating the Essential Oil Composition of Rosmarinus officinalis Before and After Fertilizing with Vermicompost. Journal of Essential Oil-Bearing Plants, 20 (5), 1413–1417.
Govindarajan, M., Rajeswary, M., Hoti, S. L., & Benelli, G. (2016). Larvicidal potential of carvacrol and terpinen-4-ol from the essential oil of Origanum vulgare (Lamiaceae) against Anopheles stephensi, Anopheles subpictus, Culex quinquefasciatus and Culex tritaeniorhynchus (Diptera: Culicidae). Research in veterinary science, 104, 77-82.
Hammer, K. A., Carson, C. F., Riley T. V. (2012). Effects of Melaleuca alternifolia (tea tree) essential oil and the major monoterpene component terpinen-4-ol on the development of single-and multistep antibiotic resistance and antimicrobial susceptibility. Antimicrobial Agents and Chemotherapy, 56, (2), 909-915.
Helander, I. K., Alakomi, H. L., Latva‐Kala, K., Mattila‐Sandholm, T., Pol, I., Smid, E. J., Von Wright, A. (1998). Characterization of the action of selected essential oil components on Gram negative bacteria. Journal of Agricultural Chemistry, 46 , 3590– 3595.
Holetz, F. B., Pessini, G. L., Sanches, N. R., Cortez D. A., Nakamura, C. V., Filho, B. P. (2002). Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Meml Inst Oswaldo Cruz 97,1027- 1031.
Kazimierczak, R., Hallamann, E., Rembialkowska, E. (2015). Effects of organic and conventional production systems on the content of bioactive substances in four species of medicinal plants. Biological Agriculture and Horticulture, 31 (2), 118–127.
Khan, M., Khan, S. T., Khan, M., Mousa, A. A., Mahmood, A., & Alkhathlan, H. Z. (2019). Chemical diversity in leaf and stem essential oils of Origanum vulgare L. and their effects on microbicidal activities. AMB Express, 9 (1), 176.
Kosakowka, O.; Weglarz, Z.; Baczek, K. (2019). Yield and quality of ‘Greek oregano’ (Origanum vulgare L. subsp. hirtum) herb from organic production system in temperate climate. Industrial Crops and Products, 141, 111782.
Laothaweerungsawat, N., Sirithunyalug, J., Chaiyana, W. (2020). Chemical Compositions and Anti-Skin-Ageing Activities of Origanum vulgare L. Essential oil from tropical and Mediterranean region. Molecules, 25 (5), 1101.
Leite, P. A. K. (2016). Selection of rhizobacterium as growth promoters in watermelon. Scientia Plena, 12 (4), 1- 8..
Li, W. R., Li, H. L., Shi, Q. S., Sun, T. L., Xie, X. B., Song, B., & Huang, X. M. (2016). The dynamics and mechanism of the antimicrobial activity of tea tree oil against bacteria and fungi. Applied microbiology and biotechnology, 100 (20), 8865-8875.
Lu, M. et al. (2018). Bactericidal property of oregano oil against multidrug-resistant clinical isolates. Frontiers in microbiology, 9, 2329.
Lukas, B., Novak, J. (2013). The complete chloroplast genome of Origanum vulgare L. (Lamiaceae). Gene, 528 (2), 163–169.
Lukas, B., Schmiderer, C., Novak, J. (2015). Essential oil diversity of European Origanum vulgare L. (Lamiaceae). Phytochemistry, 119, 32–40.
Luz, J. M. Q., et al (2014). Production of Melissa officinalis L. essential oil at different times, cultivation systems and fertilization. Brazilian Journal of Medicinal Plants, 16 (3), 552–560.
Manohar, V., Ingram, C., Gray, J., Talpur, N. A., Echard, B. W., Bagchi, D., & Preuss, H. G. (2001). Antifungal activities of origanum oil against Candida albicans. Molecular and cellular biochemistry, 228 (1-2), 111-117.
Mastro, G. De et al. (2017). Essential oil diversity of Origanum vulgare L. populations from Southern Italy. Food Chemistry, 235, 1–6.
Mondello, F., De Bernardis, F., Girolamo, A. et al. (2006). In vivo activity of terpinen-4-ol, the main bioactive component of Melaleuca alternifolia Cheel (tea tree) oil against azole-susceptible and -resistant human pathogenic Candida species. BMC Infect Dis, 6, 158.
Morshedloo, M. R., et al. (2017). Effect of prolonged water stress on essential oil content, compositions and gene expression patterns of mono- and sesquiterpene synthesis in two oregano (Origanum vulgare L.) subspecies. Plant Physiology and Biochemistry, 111, 119–128.
Nazzaro, F., Fratianni, F., De Martino, L., Coppola, R., & De Feo, V. (2013). Effect of essential oils on pathogenic bacteria. Pharmaceuticals, 6 (12), 1451-1474.
Nikou, S., et al. (2019). Effects of organic, chemical and integrated nutrition systems on morpho-physiological traits of oregano (Origanum vulgare L.). Turkish Journal of Field Crops, 24 (1), 70–80.
Nowotarska, S. W., Nowotarski, K., Grant, I. R., Elliott, C. T., Friedman, M., & Situ, C. (2017). Mechanisms of antimicrobial action of cinnamon and oregano oils, cinnamaldehyde, carvacrol, 2, 5-dihydroxybenzaldehyde, and 2-hydroxy-5-methoxybenzaldehyde against Mycobacterium avium subsp. paratuberculosis (Map). Foods, 6 (9), 72.
Olivares, F. L. (2017). Plant growth promoting bacterium and humic substances: Crop promotion and mechanisms of action. Chemical and Biological Technologies in Agriculture, 4, .
Pathama, J., Sakthaivel, N. (2002). Microbial diversity of vermicompost bacteria that exhibit useful agricultural traits and waste management potential. SpringerPlus, 1 (1), 1–19.
Paulus, D. et al. (2013). Content and chemical composition of essential citron oil as a function of seasonality and harvest time. Brazilian Horticulture, 31 (2), 203–209.
Pereira, M. M. A., et al. (2019). Humic Substances and Efficient Microorganisms: Elicitation of Medicinal Plants—A Review. Journal of Agricultural Science, 11 (7), 268.
Possamai, M. C. F., dos Santos, I. C., Silva, E. S., Gazim, Z. C., Gonçalves, J. E., Soares, A. A., & Otutumi, L. K. (2019). In vitro bacteriostatic activity of Origanum vulgare, Cymbopogon citratus, and Lippia alba essential oils in cat food bacterial isolates. Semina: Ciências Agrárias, 40(6Supl2), 3107-3122.
Prabha, K., Padmavathiamma, A., Loretta, Y., Li, B., Usha R, Kumari. (2008). An experimental study of vermi-biowaste composting for agricultural soil improvement. Bioresource Technology. 99, 1672-1681.
Pradebon B. L., et al. (2018). Evaluation of anti-enzyme properties of Origanum vulgare essential oil against oral Candida albicans. Journal de Mycologie Medicale, 28 (1), 94–100.
Rosen J. D. A. 2010. Review of the nutrition claims made by proponents of organic food. Compr Ver Food Sci Food Saf. 9, 270–277.
Sarikurkcu, C., Zengin, G., Oskay, M., Uysal, S., Ceylan, R., Aktumsek, A. (2015). Composition antioxidant, antimicrobial and enzyme inhibition activities of two Origanum vulgare subspecies (subsp. vulgare and subsp. hirtum) essential oils. Ind.Crop Prod, 70, 178–184.
Sarker SD, Nahar L, Kumarasamy Y. (2015). Microtitre plate-based antibacterial assay incorporating resazurin as na indicator of cell growth, and its application in the in vitro antibacterial screening of phytochemicals. Methods, 42, 321-324.
Sarrou, E., et al. (2017). Conventional breeding of Greek oregano (Origanum vulgare ssp. hirtum) and development of improved cultivars for yield potential and essential oil quality. Euphytica, 213, 5.
Shiwakoti, S., et al. (2016). Growing spearmint, thyme, oregano, and rosemary in Northern Wyoming using plastic tunnels. Industrial Crops and Products, 94, 251–258.
Silva, S. M., et al. (2017). Organo-mineral fertilization effects on biomass and essential oil of lavender (Lavandula dentata L.). Industrial Crops and Products, 103 (April), 133–140.
Souza, A., Zeneratto, N., Piccoli, R., & Bertolucci, S. (2018). Control of Enterotoxigenic Escherichia coli in Ground Beef by Blends of Essential Oils. Advances in Microbiology, 8, 917-930.
Souza, A. A., Dias, N. A. A., Piccoli, R. H., & Bertolucci, S. K. V. (2016). Chemical composition and minimum bactericidal concentration of sixteen essential oils on Esoterichia coli enterotoxigenic. Rev. Bras. Pl. Med. [Internet], 105-112.
Suzuki, É. Y. et al.(2015). Essential Oil from Origanum vulgare Linnaeus: An Alternative against Microorganisms responsible for Bad Perspiration Odour. Journal of Young Pharmacists, 7 (1), 12–20.
Trinh, C. S., Jeong, C. Y., Lee, W. J., Truong, H. A., Chung, N., Han, J.,Lee, H. (2018). Paenibacillus pabuli strain P7S promotes plant growth and induces anthocyanin accumulation in Arabidopsis thaliana. Plant Physiology and Biochemistry, 129, 264-272.
Tripathy, V., et al.(2015). Residues and contaminants in medicinal herbs - A review. Phytochemistry Letters, 14, 67–78.
Ultee, A., Slump, R. A., Steging, G., Smid, E. J. (2000). Antimicrobial activity of carvacrol toward Bacillus cereus on rice. Journal of Food Protection 63 (5), 620 – 624.
How to Cite
Copyright (c) 2020 Maysa Mathias Alves Pereira; Ludmila Caproni Morais; Natália Jubram Zeneratto; Willian de Souza Matias Reis; Omar Cabezas Gómez; Jaine Honorata Hortolam Luiz; Dajara Moana Barbosa Moreira; Moacir Pasqual; Joyce Dória
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.