Botanical studies, antimicrobial activity and cytotoxity of Eleutherine bulbosa (Mill.) Urb

Authors

DOI:

https://doi.org/10.33448/rsd-v9i11.9992

Keywords:

Eleutherine bulbosa; Cataphylls; Isoeleutherin; Citotoxicity; Antimicrobial activity.

Abstract

The use bulbs of Eleutherine bulbosa for treatament of diseases caused by malaria, amoeba and bacteria. This study accomplished the botanical and phytochemical characterization, antimicrobial activity and cytotoxic of E. bulbosa. For the anatomical studies, the studied material was fixed in formaldehyde – acetic acid and ethanol and it was dyed in safranin and in astra blue. For histochemistry, was fixed in buffered neutral formalin and in ferrous sulphate in formalina. Ethanolic Extract (EE) was submitted to fractionation in a chromatographic column and four (4) fractions were obtained from it. The Dichloromethane Fraction (DF) was submitted to a new fractionation. The biological activity was evalueted by diffusion in agar, microdilution and celular viability MTT. The bulb of E. bulbosa is characterized by a reduced caulinar axis and by succulent amiliferous cataphylls, epidermis with the presence of anthocyanins, homogeneous mesophyll with idioblasts of prismatic crystals of calcium oxalate and phenolic compounds. In agar diffusion it was observed that EE, DF and ethyl acetate fraction (ACF) were active for Staphylococcus aureus. In microdilution, DF (Inhibitory minimum concentration= 125 µg/ mL) was more active. For all samples the Minimous Bactericidal Concentration was >1000 µg/ mL. The fractionation contributes positively with the citotoxicity, being subfractions S1 and S2 the most citotoxic ones. The Fraction Dichloromethane was the most active one for S. aureus and more citotoxicity to VERO cells. Probably the less citotoxicity of EE is related to the presence of anthocyanins that are present on bulbs epidermis.

References

Alves, T. M. A., Kloos, H. & Zani, C. L. (2003). Eleutherinone, a novel fungitoxic naphthoquinone from Eleutherine bulbosa (Iridaceae). Mem. Inst. Oswaldo Cruz, 98 (5), 709-712. doi: 10.1590/S0074-02762003000500021.

Bauer, A. W., Kirby, W. M. M., Sherris, J. C. & Turck, M. (1966). Antibiotic susceptibilities testing by standard single disc diffusion method. American Journal Clinical Pathology, 36,493-496.

Bianchi, C. & Ceriotti, G. (1975). Chemical and pharmacological investigations of constituents of Eleutherine bulbosa (Miller) Urb. (Iridaceae). Journal of Pharmaceutical Sciences, 64 (8), 1305-1308. doi: 10.1002/jps.2600640809.

Borges, E. S. (2012). Estudos farmacognósticos, fitoquímicos e atividades biológicas de Eleutherine plicata Herb. 108f. Dissertação (Mestrado) - Universidade Federal do Pará, Instituto de Ciências da Saúde, Belém, Programa de Pós-Graduação em Ciências Farmacêuticas.

Braga, M. M. N. (1977). Leaf anatomy of Bromeliaceae of Campina. Acta Amazonica, 7, 1-74.

Coelho-Ferreira, M. (2009). Medicinal knowledge and plant utilization in an Amazonian coastal community of Marudá, Pará State (Brazil). Journal of Ethnopharmacology, 126,159–175. doi: 10.1016/j.jep.2009.07.016.

Clinical and Laboratory Standars Institute, Wayne USA (CLSI). (2012). Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically: approved standard. Ninth edition, M07 A9, 32(2).

Brandão, D. L. do N., Martins, M. T., Silva, A. O., Almeida, A. D., Paula, R. C. de ., Oliveira, A. B. de, Coelho-Ferreira, M. R., Gomes, A. T. A., Vasconcelos, F. de, Pereira, W. L. A., Vale, V. V., Percário, S., & Dolabela, M. F. . (2020). Anti-malarial activity and toxicity of Aspidosperma nitidum Benth: a plant used in traditional medicine in the Brazilian Amazon. Research, Society and Development, 9(10), e5059108817. doi: https://doi.org/10.33448/rsd-v9i10.8817.

Ding, J. & Huang, H. (1983): Preparation of Hong Cong Su tablet. Chem Abstr., 98, 113584.

Dubin, M., Fernandez Villamil, S.H. & Stoppani, A.O. (1990). Inhibition of microsomal lipid peroxidation and cytochrome P-450-catalyzed reactions by β-lapachone and related naphthoquinones. Biochemical Pharmacology, 39, 1151–1160. doi: 10.1016/0006-2952(90)90256-K.

Franceschi, V. R. & Horner Junior, H. T. (1980). Calcium oxalate crystals in plants. Brazilian Review of Botany, 46, 361-427.

Gerlach, D. (1977). Botanish Mikrotecnik. Sttugart, George. Thiemeverlog, 311.

Goldblatt, P., Henrich, J. E. & Rudall, P. (1984). Occurrence of crystals in Iridaceae and allied families and their phylogenetic signi®cance. Annals Missouri Botanical Garden, 71, 1013-1020.

Hara, H., Maruyama, N., Yamashita, S., Hayashi, Y., Lee, K. H., Bastow, K. F., Chairul, R. M. & Imakura, Y. (1997). Elecanacin, a novel new naphthoquinone from the bulbs of Eleutherine americana. Chemical Pharmacology Bulletin, 45, 1744-1716.

Holetz, F. B., Pessini, G. L., Sanches, N. R., Cortês, D. A. G., Nakamura, C. V. & Benedito Filho, P. D. (2002). Screening of some plants used in the Brasilian folk medicine for the treatment of infectious diseases. Mem. Inst. Oswaldo Cruz, 97, 1027-1031. 10.1590/S0074-02762002000700017.

Ifesan, B. & Voravuthikunchai, S. P. (2009). Effect of Eleutherine americana Merr. Extract on enzymatic activity and enterotoxin productionStaphylococcus aureus in broth and cooked pork. Foodborne Pathogens and disease, 6 (6), 699-704. doi: 10.1089/fpd.2009.0279.

Jiang, Y., Liu, X., Dai, M., Nie, W., Yang, X. & Zeng, X. (2017). Effects of the ethanol extract of black mulberry (Morus nigra L.) fruit on experimental atherosclerosis in rats. Journal of Ethnopharmacology, 200, 228-235. doi: 10.1016/j.jep.2017.02.037.

Johansen, D. A. (1940). Plant microtechnique. New York: Mcgraw-Hill 532.

Kartal, M., Yvldz, S., Kaya, S., Kurucu, S. & Topçu, G. (2003). Antimicrobial activity of propolis simples from two different regions of Anatolia. Journal of Ethnopharmacology, 86, 69-73. doi: 10.1016/s0378-8741(03)00042-4.

Krishnan, P. & Bastow, F. K. (2000). Novel mechanisms of DNA topoisomerase II inhibition by pyranonaphthoquinone derivatives - eleutherin, α lapachone, and β lapachone. Biochemical Pharmacology, 60 (9), 1367-1379. doi: 10.1016/s0006-2952(00)00437-8.

Kusuma, I. W., Kuspradini, H., Arung, E. T., Aryani, F., Min, Y. H., Kim, J. S. & Kim, Y. (2010). Biological activity and phytochemical analysis of three Indonesian medicinal plants, Murrayakoenigii, Syzygium polyanthum and Zingiber purpurea. Journal Acupuncture and Meridian Studies, 4(1), 75-79. doi: 10.1016/s2005-2901(11)60010-1.

Lin, J., Puckree, T. & Mvelase, T. P. (2002). Anti-diarrhoeal evaluation of some medicinal plants used by Zulu traditional healers. Journal of Ethnopharmacology, 79, 53-56. doi: 10.1016/S0378-8741(01)00353-1.

Malheiros, L. C. S. (2008). Isoeleuterol e isoeleuterina: potenciais marcadores químicos da tintura de Eleutherine plicata Herb (Iridaceae) e atividades microbiológica e antioxidante. 67 p. Dissertation (Masters in Pharmaceutical Sciences) – Pharmacy College, Federal University of Pará, Pará State (Brazil).

Martins, A. G., Rosário, D. L., Barros, M. N. & Jardim, M. A. G. (2005). Levantamento etnobotânico de plantas medicinais, alimentares e tóxicas da ilha do Combu, município de Belém, Estado do Pará, Brasil. Revista Brasileira de Farmácia, 86(1), 21-30.

Matos, F. J. A., & Lorenzi, H. (2002). Plantas medicinais no Brasil Nativas e exóticas cultivadas. Instituto Plantarum Publishers, Nova Odessa, São Paulo.

Mors, W. B., Rizzini, C. T., & Pereira, N. A. (2000). Medicinal Plants of Brazil. Reference Publications, Inc. Algonac, Michigan.

Mosmann, T. (1983). Rapid colorimetry assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of immunological methods, 65 (1-2), 55-63. doi: 10.1016/0022-1759(83)90303-4.

Panda, S. K., Mohanta, Y. K., Padhi, L., Park, Y. H., Mohanta, T. K. & Bae, H. (2016). Large Scale Screening of Ethnomedicinal Plants for Identification of Potential Antibacterial Compounds. Molecules, 21(3), 293. doi: 10.3390/molecules21030293.

Pereira, A. S., Shitsuka, D. M., Pereira, F. J. & Shitsuka, R. (2018). Metodologia da pesquisa científica. Santa Maria: UFSM, NTE. Retrieved from: https://repositorio.ufs m.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa - Cientifica.pdf

Pinto, L. N. & Barbosa, W. L. R. (2009). Ethnopharmacy of the county of Igarapé Miri – PA. In: Barbosa, W. L. R. (Eds.), Etnofarmácia: fitoterapia popular e ciência farmacêutica. NUMA/UFPA Publishers, Belém, 119.

Prychid, C. J. & Rudall, J. P. (1999). Calcium Oxalate Crystals in Monocotyledons: A Review of their Structure and Systematics. Annals of Botany, 84, 725-739.

Ribeiro, C. M. (2008). Avaliação da atividade antimicrobiana de plantas utilizadas na medicina popular da Amazônia. 66 f. Dissertação (Mestrado) - Universidade Federal do Pará, Instituto de Ciências da Saúde, Belém, Programa de Pós-Graduação em Ciências Farmacêuticas.

Rios, J. L., Recio, M. C. & Villar, A. (1987). Antimicrobial activity of selected plants employed in the Spanish mediterraneam area. Journal of Ethnopharmacology, 23, 127-149. Doi: 10.1002/ptr.2650030302.

Rodrigues, A. C. & Estelita, M. E. M. (2009). Diferenciação dos feixes vasculares e dos elementos traqueais no rizoma de algumas Cyperaceae. Revista Brasileira de Botânica, 32(2), 349-359.

Rudall, P. (1994). Anatomy and systematic of Iridaceae. Botanical Journal of the Linnean Society, 114, 1-21. doi: 10.1111/j.1095-8339.1994.tb01920.x.

Rudall, P. (1995). Anatomy of the monocotyledons. VIII. Iridaceae. University Press, Oxford.

Schultes, R. E., Raffauf, R. F., 1990. The Healing Florest: Medicinal and toxic plants of the Northwest Amazonia, first ed., Portland.

Santos, D. L., Silva, G. de N. F. da, Moraes, J. S., Souza, K. O. da, Rodrigues, E. M. da S., Fecury, A. A., Dendasck, C. V., Dias, C. A. G. de M., Pinheiro, M. da C. N., Moreira, E. C. de M., Araújo, M. H. M. de, da Silva, I. R., & Oliveira, E. de. (2020). Traditional herbal medicine in a community in northeastern Pará: the use of Eleutherine plicata Herb. in the treatment of Amebiasis. Research, Society and Development, 9(7), e620974539. doi: https://doi.org/10.33448/rsd-v9i7.4539.

Silva, T. L. (2012). Avaliação da Toxicidade e Estudo da Atividade do Extrato Etanólico Seco dos Bulbos de Eleutherine plicata Herb (Iridaceae) sobre Alguns Parâmetros do Estresse Oxidativo na Malária Experimental in vivo. 80f. Dissertação (Mestrado) - Universidade Federal do Pará, Instituto de Ciências da Saúde, Belém, Programa de Pós-Graduação em Ciências Farmacêuticas.

Thor, H., Smith, M. T., Hartzell, P., Bellomo, G., Jewell, S. A. & Orrenius, S. (1982). The Metabolism of Menadione (2-Methyl-1,4-naphthoquinone) by Isolated Hepatocytes. The Journal of Biological Chemistry 257(20), 12419-12425.

Triguia, M., Hsounaa, A. B., Hammamib, I., Culiolic, G., Ksantinib, M., Tounsia, S. & Jaouad, S. (2013). Efficacy of Lawsonia inermis leaves extract and its phenolic compounds against olive knot and crown gall diseases. Crop Protection, 45, 83-88. doi: 10.1016/j.cropro.2012.11.014.

Vale, V. V., Cruz, J. N., Viana, G. M. R., Póvoa, M. M, Brasil, D. S. B. & Dolabela, M. F. (2020). Naphthoquinones isolated from Eleutherine plicata herb: in vitro antimalarial activity and molecular modeling to investigate their binding modes. Medicinal Chemistry Research, 29, 487–494. DOI: 10.1007/s00044-019-02498-z.

Veiga, A. S. S. (2013) Atividade antileishmania de plantas da Amazônia. 168f. Dissertação (Mestrado) - Universidade Federal do Pará, Instituto de Ciências da Saúde, Belém, Programa de Pós-Graduação em Ciências Farmacêuticas.

Verma, R. P. (2006). Anti-Cancer activities of 1,4-Naphthoquinones: A QSAR Study. Anti-Cancer Agents in Medicinal Chemistry, 6, 489-499. doi: 10.2174/187152006778226512.

Wolter, M. (1990). Calcium oxalat-kristalle in den Knollen-hullen von Crocus L. (Iridaceae) und ihre systemat ische Bedeutung. Botanische Jahrbucher, 112, 99-114.

Wu, Q. G. & Cutler, D. F. (1985). Taxonomic, evolutionary and ecological implications of theleaf anatomy of rhizomatous Iris species. Botanical Journal of the Linnean Society, 90, 253-303. doi: 10.1111/j.1095-8339.1985.tb00385.x.

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Published

15/11/2020

How to Cite

Borges, E. S. ., Galucio, N. C. da R., Veiga, A. S. S. ., Busman, D. V. ., Lins, A. L. F. de A. ., Bahia, M. de O. ., Rissino, J. D. ., Correa, R. M. dos S. ., Burbano, R. M. R., Marinho, A. M. R., Casique, J. V. ., Percário, S., & Dolabela , M. F. . (2020). Botanical studies, antimicrobial activity and cytotoxity of Eleutherine bulbosa (Mill.) Urb. Research, Society and Development, 9(11), e3369119992. https://doi.org/10.33448/rsd-v9i11.9992

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Health Sciences