Chemical characteristics and antimicrobial activity of the essential oil of Plectranthus amboinicus (lour.) Spreng. cultivated with different fertilizers
Keywords:Plectranthus amboinicus; Essential oil; Antimicrobian activity; Organic fertilization; Chemical composition.
Objective: To evaluate if there are differences for yield, chemical composition and, consequently, antimicrobial activity of the essential oils of Plectranthus amboinicus when submitted to crops with different fertilizations in the same period of the year. Methodology: Plectranthus amboinicus was grown with two different types of fertilization for 75 days. The extraction of essential oil from the leaves was through hydrodistillation with Clevenger and the identification of its chemical constituents was performed by gas chromatography coupled with mass spectrometry. The antimicrobial activity was determined by the broth microdilution technique. Results: Variations in yield, chemical composition and antimicrobial activity of essential oil were found in samples grown using different organic fertilizers at the same time. The best results were obtained in the sample of essential oil fertilized with bovine manure, yielding 1.65% and Carvacrol (58.6%), γ-Terpinene: (15.1%) and p-Cymene: (8.2%) as majorities. Conclusion: The majorities Carvacrol, γ-Terpinene and p-Cymene were more abundant in the cultivation with organic fertilization of bovine manure type. Likewise, the best antimicrobial potential detected by the essential oil against E. coli and C. albicans was due to the cultivation of plants with organic fertilization of bovine manure type. The data suggest that this type of fertilization is adequate to produce the essential oil with better antimicrobial activity and higher quality. Therefore, it is possible to improve the yield, the concentration of the constituents and their antimicrobial response through an affordable organic fertilizer.
Adams, R. P. (2007). Identification of essential oil components by gas chromatography/mass spectrometry (Vol. 456). Carol Stream, IL: Allured publishing corporation.
Agência Nacional de Vigilância Sanitária – ANVISA (2019). Farmacopeia Brasileira (6a ed.). Brasília: ANVISA. 874. https://www.gov.br/anvisa/pt-br/assuntos/farmacopeia/farmacopeia-brasileira/arquivos/7985json-file-1
Arumugam, G., Swamy, M. K., & Sinniah, U. R. (2016). Plectranthus amboinicus (Lour.) Spreng: Botanical, Phytochemical, Pharmacological and Nutritional Significance. Molecules, 21(4), 1-26. 10.3390/molecules-21040369
Benelli, G., & Mehlhorn, H. (2018). Mosquito-borne Diseases. Springer, Dusseldorf.
CLSI (2020). Performance standards for antimicrobial susceptibility testing. Clinical and Laboratory Standards Institute (30a ed.). Wayne, Pennsylvania, USA: Clinical and Laboratory Standards Institute.
Coutinho, D. F., Borba, E. R. C., Luz, T. R. S. A., Serejo, A. P. M., Amaral, F. M. M., & Ribeiro, E. C. G. (2020). Terpenoides: Classificação e Biossíntese: CRV.
Coutinho, D. F., Dias, C. S., Barbosa-Filho, J. M., Agra, M. F., Martins, R. M., Silva, T. M., & Craveiro, A. A. (2007). Composition and molluscicidal activity of the essential oil from the stem bark of Ocotea bracteosa (Meisn.) Mez. Journal of Essential Oil Research, 19(5), 482-484. 10.1080/10412905.2007.9699958
da Silva, A. L. R., Costa, V. R. M., Ferreira, G. B., Castro, É. J. M., da Conceição Coelho, A., de., Macedo, A. O., & da Silva Bezerra, D. (2020). Análise dos padrões dos focos de queimadas por biomas do estado do Maranhão/Analysis of the patterns of fires in biomes in the state of Maranhão. Brazilian Journal of Development, 6(2), 6399-6409. https://www.brazilianjournals.com/index.php/BRJD/article/view/6745
EL-Zefzafy, M. M., Gouda, T. M. D., & El-Morsy, T. H. (2016). In vitro and in vivo growth, chemical and antimicrobial studies for Plectranthus amboinicus plant. International Journal of PharmTech Research, 9(12), 851-865.
Baser, K. H. C., & Buchbauer, G. (Eds.). (2015). Handbook of essential oils: science, technology, and applications: CRC press
Goncalves, T. B., Braga, M. A., de Oliveira, F. F., Santiago, G. M., Carvalho, C. B., e Cabral, P. B., & Nagao-Dias, A. T. (2012). Effect of subinihibitory and inhibitory concentrations of Plectranthus amboinicus (Lour.) Spreng essential oil on Klebsiella pneumoniae. Phytomedicine, 19(11), 962-968. 10.1016/j.phymed.2012.05.013
Khalid, A. K., & El-Gohary, A. E. (2014). Effect of seasonal variations on essential oil production and composition of Plectranthus amboinicus (Lour.) grow in Egypt. International Food Research Journal, 21(5), 1859. http://ifrj.upm.edu.my/21%20(05)%202014/20%20IFRJ%2021%20(05)%202014%20Khalid%20115.pdf
Luz, T. R. S. A., Leite, J. A. C., De Mesquita, L. S. S., Bezerra, S. A., Silveira, D. P. B., De Mesquita, J. W. C., Ribeiro, E. C. G., Vilanova, C. M., Ribeiro, M. N. S., Amaral, F. M. M., & Coutinho, D. F. (2020). Seasonal variation in the chemical composition and biological activity of the essential oil of Mesosphaerum suaveolens (L.) Kuntze. Industrial Crop & Products. 153, 112600. 10.1016/j.indcrop.2020.112600
Martins, W. L. D., Blanco, C. J. C., & de Melo, A. M. Q. (2020). Potencial erosivo das chuvas nos estados do Maranhão e Pará via análise da distribuição espacial da erosividade. Geoambiente On-line, (36), 1-18. 10.5216/revgeoamb.vi36.60668
Mesquita, S. B. da S., da Costa, R. S., Amorim, A. V., de Lacerda, C. F., & da Fonseca, A. M. (2017). Crescimento e composição do óleo essencial de malvariço cultivado sob salinidade e doses de biofertilizante. Revista Agro@mbiente On-line, 11(4), 315-322. firstname.lastname@example.org
National Institute of Standards and Technology - NIST (2005). Mass Spectral Library (NIST/EPA/NIH). NIST Standard Reference Database. National Institute of Standards and Technology, Gaithersburg, MD, USA. https://www.nist.gov/srd/nist-standard-reference-database-1a-v17.
Oliveira, A. M. J. de (2020). Caracterização e avaliação da atividade inseticida do óleo essencial de Hyptis marrubioides (Hortelã do Campo) em larvas de Aedes aegypti. Dissertação de Mestrado, Universidade Federal de Lavras, MG, Brasil.
Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa científica. Ed. UAB/NTE/UFSM. https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.
Prasad, N., Basalingappa, K. M., Gopenath, T. S., Razvi, S. M., Murugesan, K., Ashok, G., & Divyashree, K. C. (2020). Nutritional Significance Of Indian Borage (Plectranthus amboinicus): A Review. Plant Archives, 20(2), 3727-3730. https://www.researchgate.n et/publication/345903386_NUTRITIONAL_SIGNIFICANCE_OF_INDIAN_BORAGE_PLECTRANTHUS_AMBOINICUS_A_REVIEW
Punet Kumar, S., & Kumar, N. (2020). Plectranthus amboinicus: a review on its pharmacological and pharmacognostical studies. American Journal of Physiology, 10(2), 55-62. 10.5455/ajpbp.20190928091007
Rodrigues, T. S., Guimarães, S. F., Rodrigues-Das-Dôres, R. G., & Gabriel, J. V. (2011). Métodos de secagem e rendimento dos extratos de folhas de Plectranthus barbatus (boldo-da-terra) e P. ornatus (boldo-miúdo). Revista Brasileira de Plantas Medicinais, 13(SPE), 587-590. 10.1590/S1516-05722011000500014
Ruan, T. Z., Kao, C. L., Hsieh, Y. L., Li, H. T., & Chen, C. Y. (2019). Chemical Constituents of the Leaves of Plectranthus amboinicus. Chemistry of Natural Compounds, 55(1), 124-126. 10.1007/s10600-019-02631-9
Santos, N. O. D., Mariane, B., Lago, J. H. G., Sartorelli, P., Rosa, W., Soares, M. G., & Pascon, R. C. (2015). Assessing the chemical composition and antimicrobial activity of essential oils from Brazilian plants—Eremanthus erythropappus (Asteraceae), Plectrantuns barbatus, and P. amboinicus (Lamiaceae). Molecules, 20(5), 8440-8452. doi.org/10.3390/molecules20058440
Sany, H., Said-Al Ahl, H. A., Mauro, R. P., & Astatkie, T. (2020). Effects of NaCl on growth, essential oil and chemical composition of Plectranthus amboinicus. Plant Archives, 20(1), 2471-2477. https://www.researchgate.net/publication/340952770_EFFECTS_ OF_NACL_ON_GROWTH_ESSENTIAL_OIL_AND_CHEMICAL_COMPOSITION_OF_PLECTRANTHUS_AMBOINICUS
Zouari-Bouassida, K., Trigui, M., Makni, S., Jlaiel, L., & Tounsi, S. (2018). Seasonal variation in essential oils composition and the biological and pharmaceutical protective effects of Mentha longifolia leaves grown in Tunisia. BioMed Research International, 18(1), 1-12. 10.1155/2018/7856517
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Copyright (c) 2021 Elizabeth Regina de Castro Borba; Thiago Castro Mubárack; Tássio Rômulo Silva Araújo Luz; Daniella Patrícia Brandão Silveira; Ana Zélia Silva; Patrícia de Maria Silva Figueiredo; Odair dos Santos Monteiro; Yan Michel Lopes Fernandes; Maria do Livramento de Paula; Crisálida Machado Vilanova; Denise Fernandes Coutinho
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