Characterization of volatile compounds and bioactive compounds of pulp and jelly of cagaita by solid phase microextraction in the headspace mode and mass spectrometry by paper spray

Authors

DOI:

https://doi.org/10.33448/rsd-v10i1.11735

Keywords:

Eugenia dysenterica; Volatile compounds; Bioactive compounds; Mytaceae.

Abstract

The cagaiteira (Eugenia dysenterica) belonging to the Myrtaceae family, is a tree native to the Brazilian Cerrado, its fruit is very fragile, with thin bark and juicy pulp, it is consumed in the processed form as a jelly to prolong its useful life. The present work had as objective to elaborate cagaita jelly and to evaluate the alteration of bioactive compounds and volatile compounds in relation to the pulp in natura. The analysis of mass spectrometry by paper spray in positive and negative modes allowed the identification of several substances, among them, organic acids, sugars, anthocyanins, hydroxycinnamic acids, hydroxybenzoic acids and flavonoids, indicating that the production of jelly can be a technique for conservation of phenolic compounds with their respective sensory qualities such as color and flavor. In the analysis of volatiles by solid phase microextraction in headspace mode, monoterpenes, hydrocarbons and sesquiterpenes were detected, indicating that the production of jelly can preserve the characteristic aroma of the fruit. In the characterization of volatile compounds in cagaita pulp, 3-carene was the major monoterpene found. While processing jelly there was production of furaldehyde, a product obtained as a natural consequence of heating.

References

Aaaby, K., Ekeberg, D. & Skrede, G. (2007). Characterization of phenolic compounds in strawberry (Fragaria x ananassa) fruits by different HPLC detectors and contribution of individual compounds to total antioxidant capacity. J. Agric. Food Chem., 55 (11), 4395-4406.

Abu-Reidah, I. M., Ali-Shtayeh, M. S., Jamous, R. M., Arráezromán, D. & Segura-Carretero, A. (2015). Food Chem., 166, 179-191.

Asakawa, D., Hiraoka, K. (2010). Rapid Commun. Mass Spectrom. 24, 2431.

Bagetti, M. (2009). Caracterização físico-química e capacidade antioxidante de pitanga (Eugenia uniflora L.). Dissertação de mestrado, Centro de Ciências Rurais, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil.

Barros, L., Dueñas, M., Carvalho, A. M., Ferreira, I. C. F. R. & Santos-Buelga, C. (2012). Characterization of phenolic compounds in flowers of wild medicinal plants from Northeastern Portugal. Food Chem. Toxicol., 50 (5), 1576-1582.

Beecher, G. R. (2003). Overview of dietary flavonoids: nomenclature, occurrence and intake. Journal of the Nutrition, 133 (10), 3248S-3254S.

Borges, K. C. (2015). Pitanga (Eugenia uniflora) desidratada por atomização e liofilização: Características físico-químicas, compostos bioativos e efeito sobre longevidade, estresse oxidativo e neurotoxicidade induzidas em modelos in vivo Caenorhabditis elengns. Tese de Doutorado, Universidade Federal do Rio Grande do Norte, Natal, RN, Brasil.

Botondi, R., Desantis, D., Bellicontro, A., Vizovitis, K. & Mencarelli, F. (2003). Influence of ethylene inhibition by 1-methylcyclopropene on apricot quality, volatile production, and glycosidase activity of low-and high-aroma varieties of apricots. Journal of Agricultural and Food Chemistry, 51 (5), 1189-1200.

Bueno, G. H., Guedes, M. N. S, Souza, A. G., Madeira, A. P. C, Garcia, E. M., Taroco, H. A. & Melo, J. O. F. (2017). Caracterização física e físico-química de frutos de Eugenia Dysentérica DC, originados em reigião de clima tropical de altitude. Ver. Bras. Biom., 35 (3), 515-522.

Carvalho, A. R. F., Oliveira, J., Freitas, V. de, Mateus, N. & Melo, A. (2010). A theoretical interpretation of the color of two classes of pyranoanthocyanins. Journal of Molecular Structure: Theochem., 948, 61-64.

Carvalho, T. C. (2015). Paperspray ionization: análise direta de licores do processo de etanol 2G por espectrometria de massas. Dissertação de mestrado, Universidade Federal de Goiás, Goiânia, GE, Brasil.

Celli, G. B., Pereira-Neto, A. B. & Beta, T. (2011). Comparative analysis de total phenolic contente, antioxidante activy, and flavonoids profile of fruits from two varieties of Brazilian cherry (Eugenia uniflora, L) throughout the fruit developmental stages. Food research international, 44 (8), 2442-2451.

Chen, H. J., Inbaraj, B. S. & Chen, B. H. (2012). Determination of phenolic acids and flavonoids in Taraxacum formosanum Kitam by liquid chromatography-tandem mass spectrometry coupled with a post-column derivatization technique. International Journal of Molecular Sciences, 13 (1), 260-285.

Chen, R., Wang, L., Xiong, C., Zhou, Y., Zhen, C., Zhang, N., Tang, Y., Zhou, X., Wang, J., Nie, Z. & Chen, Y. (2011). Direct analysis of oligosaccharides and alpha hydroxy acids in fruits using electrosonic spray ionization mass spectrometry. Analyst, 136 (18), 3809-3814.

Damiani, C. (2009) Caracterização e agregação de valor aos frutos do cerrado: Araçá (Psidium guineensis Sw.) e Marolo (Annona Crassiflora Mart.). Tese de doutorado, Universidade Federal de Lavras, Lavras, MG, Brasil.

Degáspari, C. H. & Waszczynskyj, N. (2004). Propriedades antioxidantes de compostos fenólicos. Visão acadêmica, 5 (1), 33-40.

Dorman, H. J. D. (1999). Phytochemistry and bioactive properties of pant volatile oils: antibacterial, antifungal and antioxidant activities. Tese de doutorado, University of Strathclyde, Glasgow, UK.

Evangelista, R. M. & Vieites, R. L. (2006). Avaliação da Qualidade de Polpa de Goiaba Congelada, Comercializada na Cidade de São Paulo. Segurança Alimentar e Nutricional, 13 (2), 76-81.

Flores, G., Dastmalchi, K., Paulino, S., Whalen, K., Dabo, A. J., Reynertson, K. A., Foronjy, R. F., D’armiento, J. M. & Kennelly, E. J. (2012). Food Chem., 134 (3), 1256-1262.

Franco, M. R. B. (2003). Aroma e sabor de alimentos: temas atuais. Livraria Varela.

Garcia, Y. M., Guedes, M. N. S., Rufini, J. C. M., Souza, A. G., Augusti, R. & Melo, J. O. F. (2016). Volatile compounds identified in Barbados Cherry, BRS-366 Jaburú. Scientific Electronic Archives, 9(3), 67-73.

García, Y. M., Rufini, J. C. M., Campos, M. P., Guedes, M. N. S., Augusti, R. & Melo, J. O. F. (2019). SPME Fiber Evaluation for Volatile Organic Compounds Extraction from Acerola. Journal of the brazilian chemical society, 30 (2), 247-255.

Guedes, M. N., Rufini, J. C. M., Marques, T. R., Melo, J. O. F., Ramos, M. C. P. & Viol, R. E. (2017). Minerals and phenolic compounds of cagaita fruits at different maturation stages (Eugenia dysenterica). Rev. Bras. Frutic., 39 (1).

Gogichaeva, N. V., Williams, T. & Alterman, M. A. (2007). MALDI TOF/TOF tandem mass spectrometry as a new tool for amino acid analysis. J. Am. Soc. Mass Spectrom., 18 (2), 279-284.

Gordon, A., Jungfer, E., Silva, B. A., Maia, J. G. S. & Marx, F. (2011). Phenolic constituents and antioxidant capacity of four underutilized fruits from the Amazon region. J. Agric. Food Chem., 59 (14), 7688-7699.

Guo, Y., Gu, Z., Liu, X., Liu, J., Ma, M., Chen, B., Wang, L. (2017). Phytochem. Anal., 28, 344.

Kajdzanoska, M., Gjamovski, V. & Stefova, M. (2010). HPLC-DAD-ESI-MS Identification of phenolic compounds in cultivated strawberries from Macedonia. Maced. J. Chem. Chem. Eng., 29 (2), 181-194.

Kataoka, H., Lord, H. L. & Pawliszyn, J. (2000). Applications of solid-phase microextraction in food analysis. Journal of Chromatography A, 880 (1-2), 35-62.

Klink, C. A. & Machado, R. B. (2005). A conservação do cerrado brasileiro. Megadiversidade, 1 (1), 147-155.

Koolen, H. H. F., Silva, F. M. A., Gozzo, F. C., Souza, A. Q. L. & Souza, A. D. L. (2013). Antioxidant, antimicrobial activities and characterization of phenolic compounds from buriti (Mauritia flexuosa L. f.) by UPLC–ESI-MS/MS. Food Research International, 51 (2), 467-473.

Ledesma-Escobar, C. A., Priego-Capote, F. & Castro, M. D. L. (2015). Characterization of lemon (Citrus limon) polar extract by liquid chromatography–tandem mass spectrometry in high resolution mode. Journal of Mass Spectrometry, 50 (11), 1196-1205.

Lehninger, A. L., Nelson, D. L. & Cox, M. M. (2006). Princípios de bioquímica (4a Ed). Sarvier.

Lemos-Filho, J. P. (2000). Fotoinibição em três espécies do cerrado (Annona crassifolia, Eugenia dysenterica e Campomane¬sia adamantium) na estação seca e na chuvosa. Rev Bras Bot., 23 (1), 45-50.

Nijveldt, R. J., Nood, E. V., Hoorn, D. E. V., Boelens, P. G., Norren, K. V. & Leeuwen, P. A. V. (2001). Flavonoids: a review of probable mechanisms of action and potential applications. American Journal of Clinical Nutrition., 74 (4), p. 418-425.

Oliveira, C. T., Ramos, A. L. C. C., Mendonça, H. O. P., Consenza, G. P., Silva, M. R., Fernandes, C., Augusti, R., Melo, J. O. F., Ferreira, A. V. M. & Araújo, R. L. B. (2020). Quantification of 6-gingerol, metabolomic analysis by paper spray mass spectrometry and determination of antioxidant activity of ginger rhizomes (Zingiber officinale). Research, Society and Development, 9 (8).

Oliveira, É. M. (2018). O significado do processo de modernização agrícola e os impactos ambientais em áreas de cerrado. Revista Cerrados, 16 (1), 40-58.

Ozcan, S. & Senyuva, H. Z. (2006). Improved and simplified liquid chromatography/atmospheric pressure chemical ionization mass spectrometry method for the analysis of underivatized free amino acids in various foods. J. Chromatogr. A, 1135 (2), 179-185.

Pereira, A. S., Dorlivete, M. S., Parreira, F. J., Shitsuka, R. (2018). Metodologia da pesquisa científica. UAB/NTE/UFSM.

Pereira, H. V. (2016). Espectrometria de massas com ionização por paper spray combinada a métodos quimiométricos para identificação de falsificações em cervejas. Dissertação de mestrado, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil.

Proença, C., Oliveira, R. S. & Silva, A.P. (2000). Flores e frutos do cerrado. UNB.

Ramos, A. L. C. C., Mendes, D. D., Silva, M. R., Augusti, R., Melo, J. O. F., Araújo, R. L. B. & Lacerda, I. C. A. (2020). Chemical profile of Eugenia brasiliensis (Grumixama) pulp by PS/MS paper spray and SPME-GC/MS solid-phase microextraction. Research, Society and Development, 9 (7).

Ribeiro, E. M. (2011). Atividade antioxidante e polifenóis totais do fruto de cagaita (Eugenia dysenterica DC) com e sem casca. Dissertação de mestrado, Universidade Federal do Rio de Janeiro, RJ, Brasil.

Roesler, R., Catharino, R. R., Malta, L. G., Eberlin, M. N. & Pastore, G. (2007). Antioxidant activity of Annona crassiflora: Characterization of major components by electrospray ionization mass spectrometry. Food Chem., 104 (3), 1048-1054.

Santos, M. N. G. (2015). Avaliação de Polpa de Cagaita (Eugenia dysenterica DC.) Submetida ao Congelamento e atomização. Dissertação de mestrado, Universidade Federal de Goiás, Goiânia, Brasil.

Santos, B. O., Augusti, R., Melo, J. O. F., Takahashi, J. A. & Araújo, R. L. B. (2020). Optimization of extraction conditions of volatile compounds from pequi peel (Caryocar Brasiliense Camb.) using HS-SPME. Research, Society and Development, 9 (7).

Santos, P. R. G., Cardoso, L. D. M., Bedetti, S. D. F., Hamacek, F. R., Moreira, A. V. B., Martino, H. S. D. & Pinheiro-Santana, H. M. (2012). Geleia de cagaita (Eugenia dysenterica DC.): desenvolvimento, caracterização microbiológica, sensorial, química e estudo da estabilidade. Revista do Instituto Adolfo Lutz, 71 (2), 281-290.

Silva, D. B, Silva, J. A., Junqueira, N. T. V. & Andrade, L. R. M. (2001). Frutas do Cerrado. Brasília: Embrapa Informação Tecnológica.

Silva, M. R., Freitas, L. G., Souza, A. G., Araújo, R. L. B., Lacerda, I. C. A., Pereira, H. V., Augusti, R. & Melo, J. O. F. (2019). Antioxidant Activity and Metabolomic Analysis of Cagaitas (Eugenia dysenterica) using Paper Spray Mass Spectrometry. J. Braz. Chem. Soc., 30 (5), 1034-1044.

Silva, N. A., Rodrigues, E., Mercadante, A. Z. & Rosso, V. V. (2014). Phenolic compounds and carotenoids from four fruits native from the Brazilian Atlantic forest. J. Agric. Food Chem., 62 (22), 5072-5084.

Souza, E. R. B. D., Naves, R. V., Borges, J. D., Vera, R., Fernandes, E. P., Silva, L. B. & Trindade, M. D. G. (2008). Cagaiteira (Eugenia dysenterica DC.) phenology in Goiás State. Revista Brasileira de Fruticultura, 30 (4), 1009-1014.

Spínola, V., Pinto, J. & Castilho, P. C. (2015). Identification and quantification of phenolic compounds of selected fruits from Madeira Island by HPLC-DAD-ESI-MS(n) and screening for their antioxidant activity. Food Chem., 173, 14-30.

Tebaldi, V. M. R. (2008). Análise e potencial de uso de óleos essenciais no controle Pseudomonas sp. e na formação de biofilmes por Pseudomanos aeruginosa. Tese de doutorado, Universidade Federal de Lavras, Lavras, MG, Brasil.

Thomazini, M. & Franco, M. R. B. (2000). Metodologia para análise dos constituintes voláteis do sabor. Boletim SBCTA, 34 (1), 52-59.

Vendramini, A. L. & Trugo, L. C. (2000). Chemical composition of acerola fruit (Malpighia punicifolia L.) at three stages of maturity. Food Chemistry, 71 (2), 195-198.

Wang, H., Liu, J., Cooks, R. G. & Ouyang, Z. (2010). Paper spray for direct analysis of complex mixtures using mass spectrometry. Angewandte Chemie, 122 (5), 889-892.

Wang, J., Jia, Z., Zhang, Z., Wang, Y., Liu, X., Wang, L. & Lin, R. (2017). Analysis of chemical constituents of Melastoma dodecandrum Lour. By UPLC-ESI-Q-Exactive Focus-MS/MS. Molecules, 22 (3), 476-496.

Yang, Q., Wang, H., Maas, J. D., Chappell, W. J., Manicke, N. E., Cooks, R. G. & Ouyang, Z. (2012). Paper spray ionization devices for direct, biomedical analysis using mass spectrometry. International journal of mass spectrometry, 312 (15), 201-207.

Yuan, H., Wu, Y., Liu, W., Liu, Y., Gao, X., Lin, J., Zhao, Y. (2015). Carbohydr. Res., 407, 5.

Downloads

Published

12/01/2021

How to Cite

RODRIGUES, D. B. .; MENDONÇA, H. de O. P. .; NOGUEIRA, L. A. .; PAULA, A. C. C. F. F. de .; MELO, A. C. de .; REINA, L. D. C. B. .; SILVA , M. R. .; ARAÚJO, R. L. B. de .; AUGUSTI, R. .; MELO, J. O. F. . Characterization of volatile compounds and bioactive compounds of pulp and jelly of cagaita by solid phase microextraction in the headspace mode and mass spectrometry by paper spray. Research, Society and Development, [S. l.], v. 10, n. 1, p. e25610111735, 2021. DOI: 10.33448/rsd-v10i1.11735. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/11735. Acesso em: 22 jan. 2021.

Issue

Section

Agrarian and Biological Sciences