Effect of Origanum vulgare L. essential oil and carvacrol on the growth of pathogenic bacteria in the oropharynx

Authors

DOI:

https://doi.org/10.33448/rsd-v10i1.11754

Keywords:

Origanum; Disk Diffusion Antimicrobial Tests; Microbial viability.

Abstract

The aim of the study was to evaluate the effect of the essential oils of Origanum vulgare L. (EOO) and carvacrol (CAR) without growth from bacteria oropharynx. The microbiological techniques were applied: agar diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The ANOVA test was applied, with the Tukey test. The level of significance adopted was 5%. Tested strains were Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 15656, Escherichia coli ATCC 25922 and Klebsiella pneumoniae INCQS 00147. The EOO showed antimicrobial activity, varying the size of the halos from 21.00 (± 0.20) mm, for E. coli, to 33.56 (± 14.32) mm, for S. aureus. The CAR has MIC lower for K. pneumoniae and a higher concentration for S. aureus. The MBC showed similar values the MIC. Origanum vulgare L. essential oil and carvacrol showed an inhibitory effect on the growth of bacteria in the oropharynx.

References

Cabrera-Contreras, R., Morelos-Ramírez, R., Quiróz-Ríos, J. P., & Muñoz-Quiróz, D. (2020). Antibacterial Effects of Oregano Essential Oil (OEO) and Its Potential Applications. Microbiology Research Journal International, 30 (4), 19-25.

Carezzano, M. E., Sotelo, J. P., Primo, E., Reinoso, E. B., Paletti Rovey, M. F., Demo, M. S., Giordano, W.F. & Oliva, de las M. (2017). Inhibitory effect of Thymus vulgaris and Origanum vulgare essential oils on virulence factors of phytopathogenic Pseudomonas syringae strains. Plant Biology, 19 (4), 599-607.

CLSI. (2015). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically: Approved Standard. Clinical and Laboratory Standards Institute, 35(2).

Cordeiro, L.V., Figueiredo, P.T.R., Sousa, A.P., Andrade Jr, F.P., Souza, H.D.S., Araújo, D.L., Sobreira, A.L.C. & Lima, E.O. (2020). Association of carvacrol with ceftazidime and cefepime against Klebsiella pneumoniae. Research, Society and Development, 9 (7), e264974089.

Cui, H., Zhang, C., Li, C., & Lin, L. (2019). Antibacterial mechanism of orégano essential oil. Industrial Crops & Products, 139, 1-9.

Freitas, L. M. A. & Cruz, I.C.F. (2020). Nursing evidence-based practice guidelines for self-care: oral hygiene in ICU - Systematic Literature Review. Journal of Specialized Nursing Care, 12(1).

Kachur, K. & Suntres, Z. (2019). The antibacterial properties of phenolic isomers, carvacrol and thymol. Critical Reviews in Food Science and Nutrition, 60 (18), 3042-3053.

Khan, M., Khan, S. T., Khan, N. A., Mahmood, A., Al-Kedhairy, A. A., & Alkhathlan, H. Z. (2018). The composition of the essential oil and aqueous distillate of Origanum vulgare L. growing in Saudi Arabia and evaluation of their antibacterial activity. Arabian Journal of Chemistry,11(8), 1189-1200.

Magi, G., Marini, E. & Facinelli, B. (2015). Antimicrobial activity of essential oils and carvacrol, and synergy of carvacrol and erythromycin, against clinical, erythromycin-resistant Group A Streptococci. Frontiers in Microbiology, 6(165), 1-7.

NCCLS. (2003a). Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard— Eighth Edition. Clinical and Laboratory Standards Institute. M2-A8.

NCCLS. (2003b). Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria That Grow Aerobically; Approved Standard—Sixth Edition. Clinical and Laboratory Standards Institute. M7-A6.

Özalp. B., Sebgi, F. & Özcan, M. & Özcan, M. M. (2010). The antibacterial activity of essential oil of oregano (Origanum vulgare L.). Journal of Food, Agriculture and Environment, 8(2), 272-274.

Rattanachaikunsopon, P. & Phumkahchorn, P. (2009). Shallot (Allium ascalonicum L.) oil: Diallyl sulfide content and antimicrobial activity against food-borne pathogenic bacteria. African Journal of Microbiology Research, 3(11), 747-750.

Santos, J. R. N. dos, Teles A. M., Ferreira, C. G. & Mouchrek, A. N. (2020). Evaluation of the bactericidal and antioxidant activity of essential oil and hydroalcoholic extract of oregano (Origanum vulgare). Research, Society and Development, 9 (10), e7829108410.

Tardugno, R., Pellati, F., Iseppi, R., Bondi, M., Bruzzesi, G., & Benvenuti, S. (2017). Phytochemical composition and in vitro screening of the antimicrobial activity of essential oils on oral pathogenic bacteria. Natural Product Research, 32(5), 544-551.

Tavares, A. G., Monte, D. F. M. D., Albuquerque, A. D. R., Sampaio, F. C., Magnani, M., Siqueira Júnior, J. P. D., & Souza, E. L. D. (2015). Habituation of enterotoxigenic Staphylococcus aureus to Origanum vulgare L. essential oil does not induce direct-tolerance and cross-tolerance to salts and organic acids. Brazilian Journal of Microbiology, 46(3), 835-840.

Vasconcelos, N. G., Croda, J., Silva, K. E., Motta, M. L. L., Maciel, W. G., Limiere, L. C., & Simionatto, S. (2019). Origanum vulgare L. essential oil inhibits the growth of carbapenem-resistant gram-negative bacteria. Revista da Sociedade Brasileira de Medicina Tropical, 52, e2018050.

Wijesundara, N. M. & Rupasinghe. H. P. V. (2018). Essential oils from Origanum vulgare and Salvia officinalis exhibit antibacterial and anti-biofilm activities against Streptococcus pyogenes. Microbial Pathogenesis, 117, 118-127.

Xiao, S., Cui, P., Shi, W., & Zhang, Y. (2020). Identification of essential oils with activity against stationary phase Staphylococcus aureus. BMC complementary medicine and therapies, 20(1), 1-10.

Published

23/01/2021

How to Cite

LEUTHIER , L. L.; SILVA, E. C. A. da .; ALMEIDA JÚNIOR, A. .; NUNES, J. M. F. F. .; SAMPAIO, F. C. .; FARIAS, I. A. P. Effect of Origanum vulgare L. essential oil and carvacrol on the growth of pathogenic bacteria in the oropharynx. Research, Society and Development, [S. l.], v. 10, n. 1, p. e45210111754, 2021. DOI: 10.33448/rsd-v10i1.11754. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/11754. Acesso em: 28 feb. 2021.

Issue

Section

Health Sciences