Phytochemical screening, in vitro antioxidant, photoprotective and hemolytic activities of ethyl acetate extracts of the fruits and branches from Spondias tuberosa (umbu)

Authors

DOI:

https://doi.org/10.33448/rsd-v10i1.11825

Keywords:

Spondias tuberosa; Umbu; Fotoproteção; Atividade hemolítica; Antioxidantes.

Abstract

Spondias tuberosa is a medicinal plant used to treat inflammatory conditions and digestive disorders. The phytochemical screening, antioxidant, photoprotective and hemoytic activities of ethyl acetate extracts from the fruits and branches were investigated. Antioxidant activities were evaluated using DPPH, ABTS and phosphomolybdenum assays. The photoprotective and hemolytic activities was evaluated by the spectrophotometric method. Identification of compounds classes from the ethyl acetate branches extract was performed by HPLC. TLC analysis demonstrated presence of flavonoids, cinnamic derivatives, triterpenes and steroids in both extracts, and proanthocyanidins and leucoanthocyanidins only in branches, and it was identified by HPLC gallic acid, chlorogenic acid, caffeic acid, ferulic acid in the ethyl acetate extract of branches. Ethyl acetate branches extracts showed the best results: total phenolic compounds (83.88 ± 0.2 mg GAE/g), flavonoids (11.24 ± 2.0 mg QE/g), DPPH (88.80 ± 0.4 %), ABTS (68.92 ± 0.7%), phosphomolybdenum (27.94 ± 0.26%) and sun protection factor (SPF) with 15.50 ± 0.41. In addition, both extracts did not present hemolytic activity. Therefore, the branches extracts can be used in the production of photoprotective application with phytocosmetics. This work is the first report of photoprotective activity of Spondias tuberosa (umbu).

References

Brand-Williams, W., Cuvelier, M. E., & Berset, C. L. W. T. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, 28(1), 25-30.

Brazilian Health Regulatory Agency (ANVISA). RDC No 30, June 2012. “Approves the Mercosur Technical Regulation on Sunscreens in Cosmetics and other measures”. ANVISA: Brasilia, Brazil, 2012.

Cooper-Driver, G. A. (2001). Contributions of Jeffrey Harborne and co-workers to the study of anthocyanins. Phytochemistry, 56(3), 229-236.

Costa, S. C., Detoni, C. B., Branco, C. R., Botura, M. B., & Branco, A. (2015). In vitro photoprotective effects of Marcetia taxifolia ethanolic extract and its potential for sunscreen formulations. Revista Brasileira de Farmacognosia, 25(4), 413-418.

De-Qiang, D., Zheng, X., Guang, Y., Jian-Feng, Z., Ying-Kun, Q., Jing-Xian, Y., & Ting-Guo, K. (2011). Prediagnostic methods for the hemolysis of herbal medicine injection. Journal of ethnopharmacology, 138(2), 445-450.

Deuschle, V. C. K. N., Deuschle, R. A. N., Bortoluzzi, M. R., & Athayde, M. L. (2015). Physical chemistry evaluation of stability, spreadability, in vitro antioxidant, and photo-protective capacities of topical formulations containing Calendula officinalis L. leaf extract. Brazilian Journal of Pharmaceutical Sciences, 51(1), 63-75.

Feng, R., Lu, Y., Bowman, L. L., Qian, Y., Castranova, V., & Ding, M. (2005). Inhibition of activator protein-1, NF-κB, and MAPKs and induction of phase 2 detoxifying enzyme activity by chlorogenic acid. Journal of Biological Chemistry, 280(30), 27888-27895.

Granado-Serrano, A. B., Angeles Martín, M., Izquierdo-Pulido, M., Goya, L., Bravo, L., & Ramos, S. (2007). Molecular mechanisms of (−)-epicatechin and chlorogenic acid on the regulation of the apoptotic and survival/proliferation pathways in a human hepatoma cell line. Journal of Agricultural and Food Chemistry, 55(5), 2020-2027.

Harborne, A. J. (1998). Phytochemical methods a guide to modern techniques of plant analysis. springer science & business media.

Heo, M. Y., Kim, S. H., Yang, H. E., Lee, S. H., Jo, B. K., & Kim, H. P. (2001). Protection against ultraviolet B-and C-induced DNA damage and skin carcinogenesis by the flowers of Prunus persica extract. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 496(1-2), 47-59.

Kähkönen, M. P., Hopia, A. I., Vuorela, H. J., Rauha, J. P., Pihlaja, K., Kujala, T. S., & Heinonen, M. (1999). Antioxidant activity of plant extracts containing phenolic compounds. Journal of agricultural and food chemistry, 47(10), 3954-3962.

Li, H. B., Wong, C. C., Cheng, K. W., & Chen, F. (2008). Antioxidant properties in vitro and total phenolic contents in methanol extracts from medicinal plants. LWT-Food Science and Technology, 41(3), 385-390.

Machado, H., Nagem, T. J., Peters, V. M., Fonseca, C. S., & de Oliveira, T. T. (2008). Flavonóides e seu potencial terapêutico. Boletim do Centro de Biologia da Reprodução, 27(1/2).

Mansur, J. D. S., Breder, M. N., Mansur, M. C., & Azulay, R. D. (1986). Determination of sun protection factor by spectrophotometric methods. An Bras Dermatol, 61(0), 121-124.

Meng, S., Cao, J., Feng, Q., Peng, J., & Hu, Y. (2013). Roles of chlorogenic acid on regulating glucose and lipids metabolism: a review. Evidence-Based Complementary and Alternative Medicine, 2013.

Murray, J. C., Burch, J. A., Streilein, R. D., Iannacchione, M. A., Hall, R. P., & Pinnell, S. R. (2008). A topical antioxidant solution containing vitamins C and E stabilized by ferulic acid provides protection for human skin against damage caused by ultraviolet irradiation. Journal of the American Academy of Dermatology, 59(3), 418-425.

Neto, J. D. R. L., de Ara&ujo Uchôa, A. D., de Moura, P. A., Bezerra Filho, C. M., Ten&orio, J. C. G., da Silva, A. G., & dos Santos Correia, M. T. (2016). Phytochemical screening, total phenolic content and antioxidant activity of some plants from Brazilian flora. Journal of Medicinal Plants Research, 10(27), 409-416.

Nichols, J. A., & Katiyar, S. K. (2010). Skin photoprotection by natural polyphenols: anti-inflammatory, antioxidant and DNA repair mechanisms. Archives of dermatological research, 302(2), 71-83.

Oliveira Junior, R. G., Araujo, C. D., Souza, G. R., Guimarães, A. L., deOliveira, A. P., deLima-Saraiva, S. R. G., & Mo, A. C. S. (2013). In vitro antioxidant and photoprotective activities of dried extracts from Neoglaziovia variegata (Bromeliaceae). Journal of Applied Pharmaceutical Science, 3(1), 122.

Omena, C. M. B., Valentim, I. B., Guedes, G. D. S., Rabelo, L. A., Mano, C. M., Bechara, E. J. H., et al. (2012). Antioxidant, anti-acetylcholinesterase and cytotoxic activities of ethanol extracts of peel, pulp and seeds of exotic Brazilian fruits: antioxidant, anti-acetylcholinesterase and cytotoxic activities in fruits. Food Research International, 49(1), 334-344.

Ong, K. W., Hsu, A., & Tan, B. K. H. (2013). Anti-diabetic and anti-lipidemic effects of chlorogenic acid are mediated by ampk activation. Biochemical pharmacology, 85(9), 1341-1351.

Popim, R. C., Corrente, J. E., Marino, J. A. G., & Souza, C. A. D. (2008). Skin cancer: use of preventive measures and demographic profile of a risk group in the city of Botucatu. Ciencia & saude coletiva, 13(4), 1331-1336.

Prieto, P., Pineda, M., & Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical biochemistry, 269(2), 337-341.

Ramalho, V. C., & Jorge, N. (2006). Antioxidants used in oils, fats and fatty foods. Química Nova, 29(4), 755-760.

Rice-Evans, C., Miller, N., & Paganga, G. (1997). Antioxidant properties of phenolic compounds. Trends in plant science, 2(4), 152-159.

Roberts, E. A. H., Cartwright, R. A., & Oldschool, M. (1957). The phenolic substances of manufactured tea. I.—Fractionation and paper chromatography of water‐soluble substances. Journal of the Science of Food and Agriculture, 8(2), 72-80.

Sander, C. S., Chang, H., Salzmann, S., Müller, C. S., Ekanayake-Mudiyanselage, S., Elsner, P., & Thiele, J. J. (2002). Photoaging is associated with protein oxidation in human skin in vivo. Journal of Investigative Dermatology, 118(4), 618-625.

Silva Almeida, J. R. G., & Oliveira-Júnior, R. G. (2013). Prospecção tecnológica de fotoprotetores derivados de produtos naturais. Revista GEINTEC-Gestão, Inovação e Tecnologias, 3(1), 032-040.

Silva, G. A., de Brito, N. J. N., dos Santos, E. C. G., Lópes, J. A., & das Graças Almeida, M. (2014). Gênero Spondias: aspectos botânicos, composição química e potencial farmacológico. Journal of Biology & Pharmacy and Agricultural Management, 10(1).

Simic, M. G., & Jovanovic, S. V. (1994).In: Inactivation of oxygen radicals by dietary phenolic compounds in anticarcinogenesis. Food Phytochemicals for Cancer Prevention: Washington, 1994, p. 20.

Siqueira, E. M. S., Félix‐Silva, J., de Araújo, L. M. L., Fernandes, J. M., Cabral, B., Gomes, J. A. D. S., & Zucolotto, S. M. (2016). Spondias tuberosa (Anacardiaceae) leaves: profiling phenolic compounds by HPLC‐DAD and LC–MS/MS and in vivo anti‐inflammatory activity. Biomedical Chromatography, 30(10), 1656-1665.

Uchôa, A. D., Oliveira, W. F., Pereira, A. P., Silva, A. G., Cordeiro, B. M., Malafaia, C. B.,et al.(2015). Antioxidant activity and phytochemical profile of Spondias tuberosa Arruda leaves extracts. American Journal of Plant Sciences, 6(19), 3038.

Vilela, F. M. P., Fonseca, Y. M., Vicentini, F. T., Fonseca, M. J. V., & Amaral, M. D. P. H. D. (2011). Determination of three ultraviolet filters in sunscreen formulations and from skin penetration studies by high-performance liquid chromatography. Quimica Nova, 34(5), 879-883.

Violante, I. M., Souza, I. M., Venturini, C. L., Ramalho, A. F., Santos, R. A., & Ferrari, M. (2009). Avaliação in vitro da atividade fotoprotetora de extratos vegetais do cerrado de Mato Grosso. Revista Brasileira de Farmacognosia, 19(2A), 452-457.

Woisky, R. G., & Salatino, A. (1998). Analysis of propolis: some parameters and procedures for chemical quality control. Journal of apicultural research, 37(2), 99-105.

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Published

19/01/2021

How to Cite

ARAÚJO , A. D. de .; OLIVEIRA, F. G. da S. .; LACERDA, F. F. .; SILVA, C. E. S. da .; SANTOS, B. S. dos .; BEZERRA-FILHO, C. M. .; RAMOS, B. de A. .; HARAND, W. .; SILVA, N. H. da .; PAIVA , P. M. G. .; ALMEIDA, J. R. G. da S. .; SILVA, M. V. da .; CORREIA , M. T. dos S. . Phytochemical screening, in vitro antioxidant, photoprotective and hemolytic activities of ethyl acetate extracts of the fruits and branches from Spondias tuberosa (umbu) . Research, Society and Development, [S. l.], v. 10, n. 1, p. e38610111825, 2021. DOI: 10.33448/rsd-v10i1.11825. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/11825. Acesso em: 12 nov. 2024.

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Agrarian and Biological Sciences