Antimicrobial activity of Spondias mombin L. aqueous and hydroethanolic extracts on Enterococcus faecalis and Pseudomonas aeruginosa - an in vitro study

Authors

DOI:

https://doi.org/10.33448/rsd-v10i1.11949

Keywords:

Phytotherapy; Anti-Infective Agents; Microbiology.

Abstract

Objective: To chemically characterize Spondias mombin L. extracts and to evaluate the in vitro antimicrobial activity of these extracts on Enterococcus faecalis and Pseudomonas aeruginosa. Material and methods: Both extracts were submitted to phytochemical prospecting and thin layer chromatography (TLC) analysis prior to determination of Minimum Inhibitory Concentration (MIC), Minimum Inhibitory Adhesion Concentration (MIAC) and Bactericidal Kinetics (BK). The Student’s t-test was used to compare MIC results at the 5% significance level. The chemical characterization, MIAC and BK data were analyzed descriptively. Results: Phenolic compounds, flavonoids, tannins and saponins were detected in the extracts. The hydroethanolic extracts in different concentrations showed statistically superior inhibitory bacterial growth activity (p <0.05) compared to the control against the two investigated microorganisms, whereas the aqueous extracts (also in different concentrations) was statistically superior (p <0.05) to the control only against Pseudomonas aeruginosa. The anti-adherent effect of both extracts was superior to that of the control for P. aeruginosa, and similar to that for E. faecalis. In their crude and diluted concentrations, aqueous and hydroethanolic extracts showed bactericidal action within the first two to four hours of contact with the bacteria. Conclusion: Spondias mombin L. extracts in different concentrations showed superior antimicrobial activity than 0.12% chlorhexidine digluconate against the microorganisms with this activity associated to the chemical characterization of these extracts.

Author Biographies

Rivanaldo Adriano Holanda Alves, Federal University of Rio Grande do Norte

 

 

Juliana de Sousa Lima Mayer, Federal University of Rio Grande do Norte

 

 

Maria Regina Macêdo Costa, Federal University of Rio Grande do Norte

 

 

Emanuelle Louyde Ferreira de Lima, Federal University of Rio Grande do Norte

 

 

Silvana Maria Zucolotto Langassner, Federal University of Rio Grande do Norte

 

 

 

Fábio Roberto Dametto, Federal University of Rio Grande do Norte

 

 

Ruthinéia Diógenes Alves Uchoa Lins, Federal University of Rio Grande do Norte

 

 

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Published

28/01/2021

How to Cite

LIMA, I. P. A. de .; ALVES, R. A. H. .; MAYER, J. de S. L. .; COSTA, M. R. M. .; MENDONÇA, A. K. P. de .; LIMA, E. L. F. de .; LANGASSNER, S. Z. .; DAMETTO, F. R. .; LIMA, K. C. de .; LINS, R. D. A. U. . Antimicrobial activity of Spondias mombin L. aqueous and hydroethanolic extracts on Enterococcus faecalis and Pseudomonas aeruginosa - an in vitro study. Research, Society and Development, [S. l.], v. 10, n. 1, p. e50710111949, 2021. DOI: 10.33448/rsd-v10i1.11949. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/11949. Acesso em: 26 feb. 2021.

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Section

Health Sciences