Evaluation of expression of messenger RNA (RNAm) of the key proteins of the route of the microRNA (miRNA) in RD cells infected with the Mayaro Virus

Authors

DOI:

https://doi.org/10.33448/rsd-v10i1.12035

Keywords:

Mayaro; MicroRNA; Dicer; Drosha.

Abstract

Introduction: The Mayaro virus (MAYV) is an arbovirus belonging to the Togaviridae family of the alphavirus genus, which is transmitted by hematophagous arthropods to vertebrate hosts, whereas non-human primates are considered primary reservoirs. Objective: To evaluate the expression of messenger RNAs (mRNA) of crucial proteins in the microRNA pathway and inflammatory cytokines in human cells during infection by the MAYV. Materials and Methods: Viral samples propagated in RD cell cultures. The RNAs were collected and extracted at predetermined times. The total RNA was then used to detect mRNA by PCR in real time (qRT-PCR). Results: the Mayaro virus RNA was verified in the cells during all the days of infection. The AgR2 mRNA protein dropped sharply by 2 dpi and 3 dpi. Conclusion: The present study demonstrated the reduction of proteins in infection with the Mayaro virus.

Author Biographies

Paloma Daguer Ewerton dos Santos, Evandro Chagas Institute

Biomedical graduate from the Federal University of Pará (UFPA), registered with the Regional Council of Biomedicine of the 4th Region with a master's degree in the area of Genetics and Molecular Biology (UFPA), with an emphasis on Bioinformatics. She served as a teacher and coordinator of the Biomedicine Course at Escola Superior da Amazônia. She has training and experience in Molecular Biology from 2004 to the present, in addition to cell culture and analysis of sequencing data. She is currently a PhD student in the Postgraduate Program in Virology at the Evandro Chagas Institute, professor of the Biomedicine course at Escola Superior Madre Celeste and immortal member of the Paraense Academy of Biomedicine

Karla Fabiane Lopes de Melo, Evandro Chagas Institute

Graduated in Bachelor of Biomedicine at Escola Superior da Amazônia (2016). Master in Virology by the Graduate Program of the Evandro Chagas Institute (2018). Doctoral student at the Biology Program for Infectious and Parasitic Agents (UFPA / IEC) working at the Viral Biogenesis laboratory in the Arbovirology and Hemorrhagic Fevers Section at Instituto Evandro Chagas. Lecturer at Escola Superior da Amazônia from 2018 to 2020. Academic consultant since 2018. Lecturer at Grupo Ser Educacional - UNAMA from April to December 2019. Lecturer at Escola Superior Madre Celeste since 2019. Adjunct Coordinator of the Biomedicine Course at Escola Superior Madre Celeste . Interest in Virology, Microbiology, Molecular Biology, Immunogenetics and Immunology.

Walter Felix Franco Neto, Evandro Chagas Institute

Graduated in Biomedicine at Escola Superior da Amazônia (2017) and a master's degree in Virology from Instituto Evandro Chagas (2020). He has experience in the area of Biochemistry and Bioinformatics, with an emphasis on Molecular Biology, acting mainly on the following topics: zika virus, NGS, transcriptome, piwi proteins, microRNAs and SARS-CoV2 molecular diagnosis.

Samir Mansour Moraes Casseb, Evandro Chagas Institute

Graduated in Biology at the University Center of the State of Pará (CESUPA) (2007), Master in Genetics and Molecular Biology at the Federal University of Pará (2011) and PhD in Virology at the Evandro Chagas Institute (2016). Reviewer of the International Medical Case Reports Journal until 2015. Full Professor at Escola Superior da Amazônia since 2013. Full Professor at Faculdade Integrada Brasil Amazônia since 2015, where he also works at the Center for Virtual Assistance (NAVI). Researcher at Instituto Evandro Chagas since 2012. Permanent Professor in the Post-Graduate Program in Virology at Instituto Evandro Chagas since 2019, where he has guidelines and co-orientations for master's and doctorate. He has experience in the area of Virology, Bioinformatics and Immunology, acting mainly on the following topics: Arbovirology, Molecular Biology, Immunology, Biostatistics, Bioinformatics and Immunogenetics.

Ana Cecília Ribeiro Cruz, Evandro Chagas Institute

Graduated in Biomedicine from the Federal University of Pará (1989), master's degree in Cellular and Molecular Biology - Department of Biochemistry and Molecular Biology (1994) and doctorate in Parasitic Biology from the Oswaldo Cruz Foundation (2005). He is currently an associate researcher in public health at the Evandro Chagas Institute, invited researcher - Energia Sustentável do Brasil SA, adjunct professor IV at the Center for Biological and Health Sciences at the University of the State of Pará, advisor for a master's and doctorate in the postgraduate program in biology of infectious and parasitic agents at the Federal University of Pará (PPGBAIP), master's and doctoral advisor of the postgraduate program in parasitic biology of the Amazon (PPGBPA) at the State University of Pará and master's and doctorate advisor of the postgraduate program in virology (PPGV) at Evandro Chagas Institute, since 2018 she has been coordinator of PPGV / IEC. She has experience in the area of ​​Microbiology, with an emphasis on Virology, working mainly on the following themes: dengue virus, flavivirus, arbovirus, dengue and aedes aegypti. Among the main lines of research, we highlight the study of experimental pathogenesis, molecular studies and genetic diversity of viruses.

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Published

31/01/2021

How to Cite

SANTOS, P. D. E. dos; MELO, K. F. L. de; FRANCO NETO, W. F. .; CASSEB, S. M. M.; CRUZ, A. C. R. Evaluation of expression of messenger RNA (RNAm) of the key proteins of the route of the microRNA (miRNA) in RD cells infected with the Mayaro Virus . Research, Society and Development, [S. l.], v. 10, n. 1, p. e55610112035, 2021. DOI: 10.33448/rsd-v10i1.12035. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/12035. Acesso em: 7 mar. 2021.

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Section

Health Sciences