Toxicological and morphological analysis of Africanized Apis mellifera selected for tolerance to the neonicotinoid thiamethoxam
DOI:
https://doi.org/10.33448/rsd-v10i2.12109Keywords:
Histology; Scanning electron microscopy; Toxicology.Abstract
Honeybees are the insect most used for pollination purposes due to its efficient characteristics for this function, which reflects in positive aspects for both nature and man. The expansion of agriculture and the development of agrochemicals to combat pests has had negative impacts on honeybee health, causing its disappearance around the world. This research aimed to evaluate the effects of honeybee exposure to the neonicotinoid insecticide thiamethoxam on its survival rate, as well as on morphological and histological changes in the midgut of adult workers from the F4 generation of Apis mellifera queens tolerant to thiamethoxam and non-tolerant worker honeybees. After the bioassays, the midgut was removed for morphological evaluation. The results showed that F4 bees were more tolerance to thiamethoxam and exhibited less significant morphological changes when compared to the non-tolerant control group.
References
Brown, M. J. & Paxton, R. J. (2009). The conservation of bee: a global perspective. Apidologie, v. 40, n. 3.
Bourgeois, A. L. et al. (2016). Molecular genetic analysis of Varroa destructor mites in brood, fallen injured mites, and worker bee longevity in honey bees. Journal of Apicultural Research, v. 54, n. 4.
Carreck, N. L. & Ratnieks, F. L. W. (2014). The dose makes the poison: have “field realistic” rates of exposure of bees to neonicotinoid insecticides been overestimated in laboratory studies? Journal of Apicultural Research, v. 53, n. 5.
Carvalho, S. M. et al. (2013). Enzymatic biomarkers as tools to assess environmental quality: a case study of exposure of the honey bee Apis mellifera to insecticides. Environmental Toxicology Chemistry, v. 32, n. 9.
Catae, A. F. et al. (2014). Cytotoxic Effects of Thiamethoxam in the Midgut and Malpighian Tubules of Africanized Apis mellifera (Hymenoptera: Apidae). Microscopy research and technique, v. 77, n. 4.
Catae, A. F. et al. (2018). Exposure to a sublethal concentration of imidacloprid and the side effects on target and nontarget organs of Apis mellifera (Apidae). Ecotoxicology, 27, n. 2.
Chambers, R. G. et al. (2019). Sub-lethal concentrations of neonicotinoid insecticides at the field level affect negatively honey yield: Evidence from a 6-year survey of Greek apiaries. PLOS ONE, v. 14, n. 4.
Cooper, D. M. et al. (2009). The insect caspases. Apoptosis, v. 14, n. 3.
Cresswell, J. E. & Thompson, H. (2012). Comment on “a common pesticide decreases foraging success and survival in honey bees”. Science, v. 337, n. 6101.
Cresswell, J. E. (2011). A meta-analisysof experiments testing the effects of a neonicotinoid insecticide on honeybees. Ecotoxicology, v. 20, n. 1.
Cruz, A. S. et al. (2010). Morphological alterations induced by boric acid and fipronil in the midgut of worker honeybee (Apis mellifera L.) larvae. Cell Biology and Toxicology, v. 26, n. 2.
Cunha, J. G. C. (2002). Melhoramento de abelhas e produção de rainhas. In: Anais do Congresso Nacional de Apicultura. Campo Grande: CBA.
Daberkow, S. & Phipps, R. (2015) Honey marketing for the commercial beekeeper. In: Graham, J. M. (eds.). The hive and the honey bee. Revised Edition. U.S.A.: Dadant and Sons, Hamilton, Illinois.
Diniz, T.O. et al. (2020) Toxicity and effects of combined agrochemical in Scaptotrigona bipunctata bees. Scientific Electronic Archives, v. 13, n. 12.
Falco, J. R. P. et al. (2010). Toxicity of thiamethoxam, behavioral effects and alterations in chromatin of Apis mellifera L, 1758 (Hymenoptera; Apidae). Research Journal Agriculture Biological Sciences, v. 6, n. 6.
Freitas, P. V. D. X. et al. (2016) Declínio populacional das abelhas polinizadoras: Revisão. Pubvet, v. 11, n. 1.
Gallai, N. et al. (2009) Economic valuation of the vulnerability of world agriculture confronted with pollinator decline. Ecological Economics, v. 68, n. 3.
Girolami, V. et al. (2009). Translocation of neonicotinoid insecticides from coated seeds to seedling guttation drops: a novel way of intoxication for bees. Journal of Economic Entomology, v. 102, n. 5.
Hassett, J. et al (2018). A significant pure population of the dark European honey bee (Apis mellifera mellifera) remains in Ireland. Journal of Apicultural Research, v. 57, n. 3.
Imperatriz-Fonseca, V. L. & Nunes-Silva, P. (2010). As abelhas, os serviços ecossistêmicos e o Código Florestal Brasileiro. Biota Neotropica, v. 10, n. 4.
Jamnik, P. et al. (2007). Antioxidative action of royal jelly in the yeast cell. Experimental Gerontology, v. 42, n. 7.
Junqueira, L. C. U & Junqueira, L. M. M. S. (1983). Basic Techniques of Cell Biology. São Paulo, Brazil: Santos.
Kerr, W. E. (1967) The history of introduction of African bees to Brazil. South African Bee Journal, v. 39, n. 1.
Kessler, S. C. et al. (2015). Bees prefer foods containing neonicotinoid pesticides. Nature, v. 10, n. 4.
Landim, C. C. (2009). Abelhas – Morfologia e função de sistemas. Ed. I. São Paulo: Editora UNESP, 408p.
Levy, S. M. et al. (2011). The role of peritrophic membrane in the resistance of Anticarsia gemmatalis larvae (Lepidoptera: Noctuidae) during the infection by its nucleopolyhedrovirus (AgMNPV). Arthropod Structure & Development, v. 40, n. 5.
Martinez, O. & Soares, A. E. E. (2012). Melhoramento genético na apicultura comercial para produção da própolis. Revista Brasileira de Saúde e Produção Animal, v. 13, n. 4.
McNeil, M. E. A. & Schmidt, J. O. (2015). Other products of the hive. In: Graham, J. M. (eds.). The hive and the honey bee. Revised Edition. U.S.A.: Dadant and Sons, Hamilton, Illinois.
Moussian, B. (2013). The apical plasma membrane of chitin-synthesizing epithelial. Insect Science, v. 20, n. 2.
Nakasu, E. Y. et al. (2014). Novel biopesticide based on a spider venom peptide shows no adverse effects on honey bees. Proceedings of the Royal Society of London B: Biological Sciences, v. 281, n. 1787.
Oliveira, A. C. et al. (2018). Pesticides affect pollinator abundance and productivity of sunflower (Helianthus annuus L.). Journal of Apicultural Research, v. 58, n. 1.
Oliveira, R. A. et al. (2014). Side‐effects of thiamethoxam on the brain and midgut of the africanized honeybee Apis mellifera (Hymenopptera: Apidae). Environmental Toxicology, v. 29, n. 10.
Pizzaia, W. C. S. et al. (2021) Apis mellifera africanized queens tolerant to the neonicotinoid thiamethoxam. Scientific Electronic Archives, v. 14, n. 2.
Queiroz, M. L. et al. (2001). Produção de Geléia Real e Desenvolvimento da Larva de Abelhas Apis mellifera, na Região Semi-Árida de Pernambuco. Revista Brasileira de Zootecnia, v. 30, n. 2.
Roat, T. C. et al. (2013). Effects of sublethaldose of fipronil on neuron metabolic activity of africanized honeybees. Archives of Environmental Comtamination and Toxicology, v. 64, n. 3.
Rossi, A. C. et al. (2013) Effects of sublethal doses of imidacloprid in malpighian tubules of africanized Apis mellifera (Hymenoptera, Apidae). Microscopy Research and Technique, v. 76, n. 5.
Sanchez-Bayo, F. et al. (2016). Are bee diseases linked to pesticides? A brief review. Environment International, v. 89, n. 1.
Seitz, N. et al. (2015). A national survey of managed honey bee 2014–2015 annual colony losses in the USA. Journal of Apicultural Research, v. 54, n. 4.
Serrão, J. E. & Landim, C. C. (2000). Ultrastructure of the midgut epithelium of Meliponinae larvae with different developmental stages and diets. Journal Apicultural, v. 39, n. 1.
Simone-Friston, M. & Spivak, M. (2010). Propolis and bee healt: the natural history and significance of resin use by honey bees. Apidologie, v. 41, n. 3.
Snodgrass, R. E. (1956). Anatomy and physiology of the honeybees. New York: Comstock Publishing Associates.
Stein, K. et al. (2017). Bee pollination increases yield quantity and quality of cash crops in Burkina Faso, West Africa. Scientific Reports, v. 7, n. 1.
Suchail, S. et al. (2001). Discrepancy between acute and chronic toxicity induced by imidacloprid and its metabolites in Apis mellifera. Environmental Toxicology and Chemistry, v. 20, n. 11.
Suryanarayanan, S. & Kleinman, D. L. (2013). Be(e)coming experts: The controversy over insecticides in the honey bee colony collapse disorder. Social Studies of Science, v. 43, n. 2.
Tarpy, D. R. et al (2010). Mating frequencies of Africanized honey bees in the south western USA. Journal of Apicultural Research, v. 49, n. 4.
Tavares, D. A. et al. (2015). In vitro effects of thiamethoxam on larvae of Africanized honey bee Apis mellifera (Hymenoptera: Apidae). Chemosphere, v. 135, n. 7.
Teixeira, A. D. et al. (2013). Degeneration and cell regeneration in the midgut of Podisus nigrispinus (Heteroptera: Pentatomidae) during post-embryonic development. Arthropod Structure Development, v. 42, n. 3.
Terra, W. R. (2001). The origin and functions of the insect peritrophic membrane and peritrophic gel. Archives Insect Biochemistry Physiology, v. 47, n. 2.
Toledo, V. A. A. & Mouro, G. F. (2005). Produção de geleia real com abelhas Africanizadas selecionadas e cárnicas híbrida. Revista Brasileira Zootecnia, v. 34, n. 6.
Williamson, S. M. et al. (2014). Exposure to neonicotinoids influences the motor function of adult worker honeybees. Ecotoxicology, v. 23, n. 8.
Zakour, M. K., & Bienefeld, K. (2015). Basic considerations in the development of breeding plans for honey bees, illustrated by data on the native Syrian honey bee (Apis mellifera syriaca). Journal of Apicultural Research, v. 53, n. 2.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 William Cristian da Silva Pizzaia; Tamiris de Oliveira Diniz; Breno Gabriel da Silva; Naiara Climas Pereira; Adriana Aparecida Sinópolis-Gigliolli; Angélica de Souza Khatlab; Eliane Gasparino; Vagner de Alencar Arnaut de Toledo; Maria Claudia Colla Ruvolo-Takasusuki
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.