Toxicological and morphological analysis of Africanized Apis mellifera selected for tolerance to the neonicotinoid thiamethoxam

Authors

DOI:

https://doi.org/10.33448/rsd-v10i2.12109

Keywords:

Histology; Scanning electron microscopy; Toxicology.

Abstract

Honeybees are the insect most used for pollination purposes due to its efficient characteristics for this function, which reflects in positive aspects for both nature and man. The expansion of agriculture and the development of agrochemicals to combat pests has had negative impacts on honeybee health, causing its disappearance around the world. This research aimed to evaluate the effects of honeybee exposure to the neonicotinoid insecticide thiamethoxam on its survival rate, as well as on morphological and histological changes in the midgut of adult workers from the F4 generation of Apis mellifera queens tolerant to thiamethoxam and non-tolerant worker honeybees. After the bioassays, the midgut was removed for morphological evaluation. The results showed that F4 bees were more tolerance to thiamethoxam and exhibited less significant morphological changes when compared to the non-tolerant control group.

Author Biographies

William Cristian da Silva Pizzaia, Universidade Estadual de Maringá

Biology degree at Faculdade Global de Umuarama (FGU). Master in Genetics and Breeding at Universidade Estadual de Maringá (UEM). PhD in Genetics and Breeding at Universidade Estadual de Maringá (UEM).

Tamiris de Oliveira Diniz, Universidade Estadual de Maringá

Biology degree at Universidade Paranaense (Unipar). Master in Genetics and Breeding at Universidade Estadual de Maringá (UEM). Specialist in Biotechnology at Universidade Estadual de Maringá (UEM). PhD student in Genetics and Breeding at Universidade Estadual de Maringá (UEM).

Breno Gabriel da Silva, Universidade Estadual de Maringá

Mathematics degree at Universidade Estadual de Maringá (UEM). Master in Biostatistics at Universidade Estadual de Maringá (UEM). PhD student in Statistics and Agricultural Experimentation at Escola Superior de Agricultura "Luiz de Queiroz" - Universidade de São Paulo (ESALQ / USP).

Naiara Climas Pereira, Universidade Estadual de Maringá

Biology degree at the Universidade Estadual de Maringá (UEM). Master in Genetics and Breeding at Universidade Estadual de Maringá (UEM). PhD student in Genetics and Breeding at Universidade Estadual de Maringá (UEM).

Adriana Aparecida Sinópolis-Gigliolli, Universidade Estadual de Maringá

PhD in Biological Sciences at Universidade Estadual de Maringá (UEM). Adjunct professor at the Department of Biotechnology, Genetics and Cell Biology at Universidade Estadual de Maringá (UEM).

Angélica de Souza Khatlab, Universidade Estadual de Maringá

PhD in Zootechnics at Universidade Estadual de Maringá (UEM).

Eliane Gasparino, Universidade Estadual de Maringá

PhD in Zootechnics at Universidade Federal de Viçosa (UFV). Associate professor at the Department of Zootechnics at Universidade Estadual de Maringá (UEM).

Vagner de Alencar Arnaut de Toledo, Universidade Estadual de Maringá

PhD in Zootechnics at Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP). Full professor at the Zootechnics Department at Universidade Estadual de Maringá (UEM).

Maria Claudia Colla Ruvolo-Takasusuki, Universidade Estadual de Maringá

PhD in Genetics and Evolution at Universidade Federal de São Carlos (UFSCAR). Associate professor at the Department of Biotechnology, Genetics and Cell Biology at Universidade Estadual de Maringá (UEM).

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Published

08/02/2021

How to Cite

PIZZAIA, W. C. da S.; DINIZ, T. de O.; SILVA, B. G. da; PEREIRA, N. C.; SINÓPOLIS-GIGLIOLLI, A. A. .; KHATLAB, A. de S. .; GASPARINO, E.; TOLEDO, V. de A. A. de .; RUVOLO-TAKASUSUKI, M. C. C. . Toxicological and morphological analysis of Africanized Apis mellifera selected for tolerance to the neonicotinoid thiamethoxam . Research, Society and Development, [S. l.], v. 10, n. 2, p. e14310212109, 2021. DOI: 10.33448/rsd-v10i2.12109. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/12109. Acesso em: 20 apr. 2024.

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Section

Agrarian and Biological Sciences