Genetic variability of Colletotrichum sublineolum through ISSR markers
DOI:
https://doi.org/10.33448/rsd-v10i2.12223Keywords:
Sorghum biocolor (L.) Moench; Anthracnose; Molecular markes.Abstract
The occurrence of diseases is a limiting factor in the development of sorghum crop. Among the diseases that causes losses in sorghum production, anthracnose is the main and most severe, mainly by the genetic variability of the pathogen. In this context, the aim of this study was to evaluate the genetic variability of Colletotrichum sublineolum isolates. DNA were extracted from 56 monosporic isolates of C. sublineolum using a DNA extraction kit, and to perform the analysis of genetic diversity of the isolates were used ISSR primers. After amplification, it was determined the polymorphic information content (PIC), allelic frequency, UPGMA and Tocher clustering analyzes and, using software Structure, the genetic structure. According to the descriptive analysis of the genetic variability of C. Sublineolum isolates, primer AP1 presented the higher value of polymorphic information content (PIC). The higher allelic frequency was observed in loci 06, 09, 10, and 24, and the lowest in locus 02. As for the clustering method, it was observed a tendency of grouping C. sublineolum isolates according the geographic origin and, in addition to demonstrating the genetic variability between the C. sublineolum isolates, it was observed the occurrence of introgression among the isolates.
References
Abbott, R., et al. (2013). Journal of Evolutionary Biology, 26(2), 229–246. https://doi.org/10.1111/j.1420-9101.2012.02599.x.
Andrade, E. M. Uesugi, C. H. Ueno, B. & Ferreira, M. A. S. V. (2007). Caracterização morfocultural e molecular de isolados de Colletotrichum gloeosporioides patogênicos ao mamoeiro. Fitopatologia Brasileira, 32(1), 21-31. https://doi.org/10.1590/S0100-41582007000100003.
B. SEIFERT
Benitez, L. C. Rodrigues, I. C. S. Arge, L. W. P. Ribeiro, M. V. & Braga, E. J. B. (2011). Análise multivariada da divergência genética de genótipos de arroz sob estresse salino durante a fase vegetativa. Revista Ciência Agronômica, 42(2), 409-416.
Bertran, I. Carvalho, F. I. F. Oliveira, A. C. Vieira, E. A. Hartwig, I. Silva, J. A.G. Shimidt, D. A. M. Valério, I. P. Busato, C. C. & Ribeiro, G. (2006) Comparação de métodos de agrupamento na representação da distância morfológica entre genótipos de trigo. Revista Brasileira Agrociência, 12(3), 279-286. https://doi.org/10.18539/CAST.V12I3.4554.
Botstein, D. White, R. L. & Skolmick, H. (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphisn. American Journal of Human Genetics, 32(3), 314-331.
Boza, E. J. Irish, B. M. Meerow, A. W. Tondo, C. L. Rodriguez, O. A. Ventura-López, M. Gomez, J. A. Moore, J. M. Zhang, D. Motamayor, J. C. & Schnell, R. J. (2013). Genetic diversity, conservation, and utilization of Theobroma cacao L.: genetic resources in the Dominican Republic. Genetic Resources and Crop Evolution 60:605–619. https://doi.org/10.1007/s10722-012-9860-4.
Caixeta, E. T. Oliveira, A. C. B. Brito, G. G. Sakiyama, N. S. (2009) Tipos de marcadores moleculares. In: BORÉM, A.; Caixeta, E. T. Marcadores moleculares. 2.ed. Viçosa: UFV. pp. 11-94.
Cardwel, K. F. Hepperly, P. R. & Frederikisen, R. A. (1989). Pathotypes of Colletotrichum graminicola and seed transmission of sorghum anthracnose. Plant Disease, 73:255-257.
Casela, C. R. & Frederiksen, R. A. (1994). Pathogenic variability in monoconidial isolates of the sorghum antracnose fungus Colletotrichum graminicola from single lesions and from monoconidial cultures. Fitopatologia Brasileira, 19(2), 149-153.
Casela, C. R. Santos, F. G. & Ferreira, A. S. (2000) Associação de patogenicidade e diversidade fenotípica de Colletotrichum graminicola, agente causal da antracnose em sorgo. Fitopatologia Brasileira, 25(3), 517‑521.
Castro, F. M. R.; Potencial Agronômico e Energético de Híbridos de Sorgo Biomassa. Lavras: UFLA, 2014. 80p. Dissertação (Mestrado em Fitotecnia).
Conab - Companhia nacional de abastecimento. Estimativa da produção de grãos no Brasil. http://www.conab.gov.br
Conab - Companhia nacional de abastecimento. Perspectiva para Agropecuária. Volume 3 – Safra 2015/2016. Produtos de Verão.
Costa, R. V. Casela, C. R. Zambolim, L. & Ferreira, A. S. (2003) A antracnose do sorgo. Fitopatologia brasileira, 28(4),345-354. https://doi.org/10.1590/S0100-41582003000400001.
Cruz, C. D. & Carneiro, P. C S. (2003) Modelos biométricos aplicados ao melhoramento genético. UFV.
Cruz, C. D. & Regazzi, A. J. (2001) Modelos biométricos aplicados ao melhoramento genético. UFV.
Cruz, C.D. (2013). GENES - a software package for analysis in experimental statistics and quantitative genetics. Acta Scientiarum, 35(3), 271-276. https://doi.org/10.4025/actasciagron.v35i3.21251.
Evanno, G. Regnaut, S. & Goudet, J. (2005). Detecting the number of clusters of individuals using the software structure: a simulation study. Molecular Ecology, 14(8), 2611-2620. https://doi.org/10.1111/j.1365-294X.2005.02553.x.
Freeman, S. Katan, T. & Sabhi, E. (1998). Characterization of Colletotrichum species responsible for anthracnose diseases of various fruits. Plant Disease, 82(6), 596-605. https://doi.org/10.1094/PDIS.1998.82.6.596.
G. HEWITT
INO
J. B. W. WOLF
J. BOUGHMAN
LA
MARCZEWSKI
Panizzi, R. C. & Fernandes, N. G. (1997). Doenças do sorgo. In: Camargo, E. A., Rezende, J. A. M. (Eds.). Manual de Fitopatologia - Doenças das Plantas Cultivadas. São Paulo: Editora Agronômica Ceres. pp. 676-689.
Parreira, D. F. Zambolim, L. Gomes, E. A. Costa, R. V. Silva, D. D. Lana, U. G. P. Neves, W. S. Figueiredo, J. E. F. & Cota, L. V. (2016). Diversidade genética estimada através de marcadores issr de Colletotrichum graminicola no Brasil. Revista Brasileira de Milho e Sorgo 15(2), 186-194. http://dx.doi.org/10.18512/1980-6477/rbms.v15n2p186-194.
Parreira, D. F. ZAmbolim, L. Gomes, E. A. Costa, R. V. Silva, D. D. Cota, L. V. Lana, U. G. P. & Silva, E. C. F. Estimativa da Diversidade Genética de Colletotrichum graminicola com Marcadores ISSR. In: XXVIII CONGRESSO NACIONAL DE MILHO E SORGO. Goiânia, 2010. Anais... Goiânia: Associação Brasileira de Milho e Sorgo. 2010. p.731-739.
Pereira, I. S. Silva, D. da D. Casela, C. R. Tardin, F. D. & ABREU, M. de S. (2011). Resistência de linhagens genitoras e híbridos simples de sorgo a Colletotrichum sublineolum, agente causal da antracnose. Revista Caatinga, 24(2), 46‑51.
Perumal, R. Ninmkayala, P. Erattamuthu, S.R. NO, Eun-Gyu.; Reddy, U.K.; Prom, L.K.; Odvody, G.N.; Luster, D.G. & Magill, C.W. (2008). Simple sequence repeat markers useful for 1-14. http://dx.doi.org/10.1186/1471-2156-9-77.
Pritchard, J. K. & Wen W. Documentation for structure software: Version 2.1, 2004. http://pritch.bsd. uchicago.edu.
R. NICHOLS
Rohlf, F. J. (1970) Adaptive hierarchical clustering schemes. Systematic Zoology, 19(1), 58-82. https://doi.org/10.1093/sysbio/19.1.58.
Rossi, F. S. Rossi, A. A. B. Dardengo, J. F. E. Brauwers, L. R. Silva, M. L. da. & Sebbenn, A. M. (2014). Diversidade genética em populações naturais de Mauritia flexuosa L. f. (Arecaceae) com uso de marcadores ISSR. Scientia Florestalis, 42(104), 631-639.
Santos, P. C. M. Lima, W. G. Bezerra, C. S. Michereff, S. J. & Câmara, M. P. S. (2015) Diversidade genotípica e patogênica de Colletotrichum musae no estado de Pernambuco. Revista Brasileira de Fruticultura 37(2), 355-366. https://doi.org/10.1590/0100-2945-034/14.
Silva, D. D. Casela, C. R. Castro, H. A. Santos, F. G. & Ferreira, A. S. (2008). Diversidade populacional de Colletotrichum sublineolum, em seis localidades no Brasil. Summa Phytopathologica, 34(2), 149-155. https://doi.org/10.1590/S0100-54052008000200007.
Silva, D. D. Resistência de híbridos de sorgo e diversidade de Colletotrichum sublineolum. Lavras: UFLA, 2009. 175 p. (Tese – Doutorado em Fitopatologia).
ST
U. DIECKMANN
Zietkiewicz, E. Rafalski, A. & Labuda, D. (1994). Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics. 20(2), 176-183. https://doi.org/10.1006/geno.1994.1151.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Thallita Santos Guimarães ; Marco Antonio Aparecido Barelli; Carla Lima Corrêa ; Valvenarg Pereira da Silva; Alex Junior Sandol Floriano ; Nilo Leal Sander ; Aline Vidor Melão Duarte ; Bruno Wagner Zago ; Jackson Lauro Borges Ribeiro ; Flávio Dessaune Tardin ; Dagma Dionísia da Silva
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
