Response to bovine tuberculin and avian tuberculin in cattle sensitized with inoculum inactivated Mycobacterium avium and Mycobacterium bovis
DOI:
https://doi.org/10.33448/rsd-v10i2.12394Keywords:
Diagnosis; PPD; Test comparison; Tuberculosis.Abstract
Tuberculosis caused by Mycobacterium bovis is an important disease in cattle e a great problem for animal health that can reach humans. For the diagnosis of the infection and the consequent sanitary measures, the National Program for Control and Eradication of Brucellosis and Tuberculosis (PNCEBT) establish the use of intradermal tuberculin tests. The aim of this study was to analyze the response to the avian and bovine tuberculin (PPD) developed by cattle sensitized with inactivated inoculum of M. avium and M. bovis. Another aim was to compare the results of the caudal fold test (CFT), the comparative cervical test (CCT), and the simple cervical test (SCT) for tuberculosis diagnosis in the sensitize animals and in animals that have not been sensitized. Repetition of the tests did not influence the proportion of positive results. There were animals sensitized with M. bovis showing reaction up to more than 500 days post sensitization. In animals sensitized with M. avium, the specificity of the CCT was higher than that of CFT and SCT, and CCT was able to discriminate the unspecific reaction induced by M. avium inoculum. In animals sensitized with M. bovis, CCT had lower sensitivity than the other two tests. The SCT and CCT cut-off with the best combination of sensitivity and specificity was lower than that adopted by the PNCEBT for the tuberculosis diagnosis in naturally infected animals. SCT hat good agreement with the other two tests, but the agreement between CFT and CCT was only moderate. The frequency of the responses to tuberculin did not show Gaussian distribution, but in some post sensitization periods or after square root or logarithmic transformation of the data.
References
Abrahão, R. M. C. M. (1998). Tuberculose humana causada pelo Mycobacterium bovis: considerações gerais e a importância dos reservatórios animais. Dissertação de mestrado em Saúde Pública, Faculdade de Saúde Pública da Universidade de São Paulo, São Paulo.
Almeida, R. F.C., Madruga, C. R., Soares, C. O.; Fernandes, M. C.; Carvalho, N. M., Jorge, K. S. G. & Osório, A. L. A. R. (2006). Resposta imune específica de bovinos experimentalmente sensibilizados com inóculos inativados de Mycobacterium bovis e Mycobacterium avium. Pesquisa Veterinária Brasileira, 26(4), 195-200.
Ameni, G., Miörner, H., Roger, F. & Tibbo, M. (2000). Comparison between comparative tuberculin and gamma-interferon tests for the diagnosis of bovine tuberculosis in Ethiopia. Tropical Animal and Production, 32, 267-276.
Brasil. (2004). Instrução Normativa SDA n.6, de 08 de janeiro de 2004. Aprova o Regulamento Técnico do Programa Nacional de Controle e Erradicação de Brucelose e Tuberculose. Diário Oficial da União, Poder Executivo, Brasília, DF, 12 jan., Seção 1, 6-10.
Brasil. (2006). Manual técnico do programa nacional de controle e erradicação da brucelose e da tuberculose animal – PNCEBT. Ministério da Agricultura, Pecuária e Abastecimento, Brasília, 184 p.
Câmera, F. P. (2016). Psiquiatria e estatística. Validação de procedimentos de diagnóstico pela curva ROC. Psychiatry on line Brasil, 21.
Casseb, A. R., Negrão, A. G., Casseb, L. M. N., Silva, S. P., Rodrigues, E. D. L., Lima, W. F. & Langoni, H. (2015). Comparação de três testes de tuberculina usados para o diagnóstico de tuberculose em búfalos de água na Ilha de Marajó. Veterinária e Zootecnia, 22(2), 231-237.
Corrêa, W. M. & Corrêa, C. N. M. (1992). Enfermidades infecciosas dos mamíferos domésticos – Tuberculose. (2a ed.), Editora Médica e Científica, 317–337.
Embrapa Gado de Leite. (2020). Pecuária de leite espera crescer cerca de 2% em 2020. https://www.embrapa.br/busca-de-noticias/-/noticia/4935845 1/pecuaria-de-leite-espera-crescer-cerca-de-2-em-2020
Guimarães, G. M., Mateus, L. S., Moraes, A. I. P.; Da Costa, W. S., Soares, N. R., Dos Santos, L. S. & Dos Santos, P. A. (2020). Qualidade do leite in natura perante a instrução normativa IN 76 do Ministério da Agricultura, Pecuária e Abastecimento do Brasil. Research, Society and Development, 9 (9), 1-19.
Jorge, K. S. G. (2001). Aplicação de testes específicos e presuntivos para o diagnóstico da tuberculose bovina no estado de Mato Grosso do Sul, Brasil. Dissertação de mestrado, FIOCRUZ/UFMS, Campo Grande, MS.
Kantor, I. N., Odeón, A. C.; Stefan, P. E.; Aianuza, M. J.; Madrid, C. R. & Marchevsky, N. (1984). Sensitivity of the cervical and the caudal fold tuberculin tests with Mycobacterium bovis in infected cattle of Argentina. Revue Scientifique et technique International Office of Epizootics, 3 (1), 137-150.
Kaya, A., Içen, H., Tuzcu, N., Simsek, A., Yesilmen, S., Yildirim, I. & Alan, A. (2015). Comparison of tuberculin skin test, IFN-γassay, real time PCR and lateral flow rapid test in diagnosis of field outbreaks of bovine tuberculosis. Kafkas University Veterinary Fak Derg, 21(5), 739-743.
Llamazares, O. R. G., Martín, C. B. G.; Nistal, D., Redondo, V. A. P., Rodríguez, L. D. & Ferri, E. F. R. (1999). Field evaluation of the single intradermal cervical tuberculin test and the interferon-gamma assay for detection and eradication of bovine tuberculosis in Spain. Veterinary Microbiology, 70, 55-66.
Martinez, E. Z., Louzada-Neto, F. & Pereira, B. B. (2003). A curva ROC para testes diagnósticos. Cadernos de Saúde Coletiva, 11 (1), 7-31.
Monaghan, M. L.; Doherty, M. L.; Collins, J. D.; Kazda, J. F. & Quinn, P. J. (1994). The tuberculin test. Veterinary Microbiology, 40, p.111-124.
Pereira, M. G. (1995). Epidemiologia. Teoria e prática. Guanabara Koogan, 596 p.
Pereira, A. S.; Shitsuka, D. M.; Parreira, F. J. & Shitsuka, R. (2018). Metodologia de Pesquisa Científica. Santa Maria: Universidade Federal de Santa Maria, 119 p.
Pinto, F. O. (2015) Métodos estatísticos e neurais para o diagnóstico da tuberculose bovina via testes cervicais. 90 f. Dissertação (Mestrado em Engenharia Elétrica) – Universidade Federal do Rio de Janeiro, Rio de Janeiro.
Pritchard, D. G. (1988). A century of bovine tuberculosis 1888-1988: conquest and controversy. Journal of Comparative Pathology, 99(4), 357-399.
Roxo, E. (1996). Tuberculose bovina: Revisão. Arquivos do Instituto Biológico, 63(2), 91-97.
Scacchia, M., Lelli, R., Petrini, A., Prencipe, V., Calistri, P & Giovannini, A. (2000). Use of innovative methods in the eradication of bovine tuberculosis. Journal of Veterinary Medicine Series B, 47, 321-327.
Sim, J. & Wright, C. C. (2005). The kappa statistic in reliability studies: Use, interpretation, and sample size requirements. Physical Therapy, 85(3), 257-268.
Siqueira, A. L. & Tibúrcio, J. D. (2011). Estatística na área da saúde: conceitos, metodologia, aplicações e prática computacional. Belo Horizonte: Coopmed, 520 p.
Stevenson, M. (2012). An R package for the analysis of epidemiological data. Version 0.9-43. Recuperado de: http://epicentre.massey.ac.nz.
Thrusfield, M. (2010). Veterinary epidemiology. Blackwell. (3a ed). 610 p.
Wood, P. R., Corner, L. A., Rothel, J. S., Ripper, J. L., McCormick, B. S., Francis, B., Melville, L., Small, K., De Witte, K., Tolson, J., Ryan, T. J., De Lisle, G. W., Cox, J. C. & Jones, J. C. (1992). A field evaluation of serological and cellular diagnostic tests for bovine tuberculosis. Veterinary Microbiology, 31, 71-79.
Váldes, R. A., Piratelli-Filho, A., Costa, H. L., Ribeiro, A. M. C. L., Nascimento, M. D. & Soares, P. M. (2014). A metrological contribution to the diagnosis of bovine tuberculosis. British Journal of Applied Science & Technology, 4(34), 4771-4790.
Varello, K., Pezzolato, M., Mascarino, D., Ingravalle, F., Caramelli, M. & Bozzetta, E. (2008). Comparison of histologic techniques for the diagnosis of bovine tuberculosis in the framework of eradication programs. Journal Veterinary Diagnostic Investigtion, 20, 64-169.
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Copyright (c) 2021 Thalita Masoti Blankenheim ; Raphaella Barbosa Meirelles-Bartoli; Glaucenyra Cecília Pinheiro da Silva; Andressa Vaz Martins; Dirceu Guilherme de Souza Ramos; Daniel Bartoli de Sousa; Nivaldo Aparecido de Assis; Luis Antonio Mathias
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