Expression profile of the PIWI mRNAs protein family in human cells experimentally infected with Dengue Virus 4
DOI:
https://doi.org/10.33448/rsd-v10i3.13371Keywords:
PIWI; mRNA; Dengue Vírus; microRNA.Abstract
Objective: Evaluate the messenger RNAs (mRNA) PIWI proteins expression during infection with VDEN 4 in human hepatocyte cells. Materials and Methods: VDEN4 strain H778494 (JQ513335) was used, which was stored in Aedes albopictus cell culture (Clone C6 / 36) at the Arbovirology and Hemorrhagic Fevers section of the Evandro Chagas Institute. The techniques of cell cultures, stock (C6/36), inoculation, extraction, viral load quantification, RTqPCR and statistical analyzes were performed at the Viral Biogenesis Laboratory. Results: According to the results obtained, two cells (HepG2 and Huh7.5) demonstrated a higher level of viral replication at 72 hours post infection (hpi). The PIWI 2 target alter its mRNA expression during VDEN4 infection. The PIWI 4 target expression, was observed an altered expression in the infected cells. Thus, it was found that they are in different poles, while the viral load in the first three days showed high expression. The expression of mRNA was low in relation to the normal rate given by the uninfected cells. Conclusion: In our findings, it was observed that PIWI2 and 4 proteins have an inverse relationship to the viral infection process by VDEN4, when there is an increase in viral replication, these two proteins end up having a significant reduction in their expression. Probably, this reduction of expression is involved with the biogenesis process of apoptosis-regulating microRNAs.
References
Amorim, M. T., Ferreira, J. F. L., Luna, F. C. F. De, Franco Neto, W. F., Melo, K. F. L. De., Araújo, A. P. S., Borges, G. A. L., Casseb, S. M. M., Cruz, A. C. R., Holanda, G. M (2020). Estudo teórico-experimental comparativo aplicado à análise da expressão gênica de formas de DNA em células de artrópodes e de mamíferos infectadas experimentalmente pelo Vírus Dengue. Research, Society and Development, [S. l.], 9 (11), 94191110687.
Baillat, D., Shiekhattar, R (2009). Functional dissection of the human TNRC6 (GW182-related) family of proteins. Molecular and cellular biology, 29 (15), 4144–55.
Franz, A. W. E., Vargas, I. S., Adelman, Z. N., Blair, C. D., Beaty, B. J., James, A. A., Olson, K. E (2006). Engineering RNA interference-based resistance to dengue virus type 2 in genetically modified Aedes aegypti. Proc. Nat. Acad. Sci. USA, 14 (103), 4198-203.
Guzman MG, Halstead SB, Artsob H, et al (2010). Dengue: a continuing global threat. Nat Rev Microbiol. 8 (12): S7-16.
Hacisuleyman, E., Cabili, M. N., Rinn, J. L (2012). A Keystone for ncRNA. Genome Biology, 13 (5) 315-321.
Johnson, B. W., Russell, B. J., Lanciotti, R. S (2005). Serotype-Specific Detection of Dengue Viruses in a Fourplex Real-Time Reverse Transcriptase PCR Assay. Journal of Clinic Microbiology, 43 (10) 4977–4983.
Karapetyan, A. R., Buiting, C., Kuiper, R. A., Coolen, M. W (2013). Regulatory Roles for Long ncRNA and mRNA. Cancers, 5 (2) 462–490.
Livak, K. J.; Schmittgen, T. D (2001). Analysis of relative gene expression data using real-time quantitative PCR and. Methods, 25, 402–408.
Umareddy, I. Pluquet, O., Wang, Q. I., Vasudevan, S. G (2007). Dengue virus serotype infection specifies the activation of the unfolded protein response. Virology journal, 4 (91), 167-176.
Van Bakel, H., Nislow, C., Blencowe, B. J., Hughues, T. R (2010). Most “dark matter” transcripts are associated with known genes. PLoS Biology, 8 (5).
Weaver, S. C, Reisen, W. K (2010). present and future arboviral threats, antivir., res., 85: 328-345.
Whitehorn, J.; Farrar, J (2010). Dengue. British Medical Bulletin, 95 (1) 161–173.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Walter Félix Franco Neto; Murilo Tavares Amorim; Karla Fabiane Lopes de Melo; Gustavo Moraes Holanda; Jardel Fábio Lopes Ferreira; Samir Mansour Moraes Casseb
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
