Discontinuous hydration improves germination and drought tolerance in Annona squamosa seedlings





Sugar apple; Dry matter; Water deficit; Growth.


The aim of this work was to determine if the discontinuous hydration of A. squamosa seeds benefit its seed germination and if the seedlings from these seeds have a greater tolerance to water deficit through the analysis of growth parameters. The experimental design was a completely randomized, in a 4x3 factorial, with four HD cycles in seeds (zero, one, two and three) and three water treatments (control with daily watering, and cycles of five and ten-intervals days between watering). Plant height, stem diameter, number of leaves, dry biomass, root/shoot ratio, leaf area, specific leaf area, and leaf area ratio were evaluated. Discontinuous hydration treatments benefited germination and promoted a better growth of the seedlings under water deficit cycles. The application of three HD cycles increased plant height, stem diameter, number of leaves, leaf area and dry matter of the seedlings compared to the other cycles. On the other hand, water suppression caused a reduction in the growth of A. squamosa, especially at ten-day intervals between watering. HD cycles in the seeds improved drought tolerance in the seedlings, mainly when three HD cycles are applied to the seeds, suggesting this is a good technique to produce seedlings better tolerant to environments with reduced water availability.

Author Biographies

José Laurindo dos Santos Júnior, Federal University of Sergipe

Graduating from the Federal University of Sergipe (UFS) since 2017. He is currently an intern and a Scientific Initiation Scholarship (CI) at the Plant Physiology and Ecophysiology Laboratory (LFEV) at the same institution at COPES (UFS). He has experience in the field of Botany, with an emphasis on Plant Physiology with emphasis on: abiotic stresses, especially water deficit, accumulation of osmotically active organic solutes and growth analysis of plants subjected to these environmental stresses. He conducts research with plants from the Brazilian semi-arid region (Caatinga). He worked on evaluating water memory in seeds of two representatives of the Annonaceae family, Annona squamosa L. and Annona muricata L. At the moment, he evaluates the effects of high temperature on seed germination and plant development in the Brazilian semiarid region. He worked with the collection, identification and conservation of botanical material. In addition, he served as Monitor of the discipline of Elements of Human Anatomy at the Federal University of Sergipe and is a member of the Group of Molecular Anatomy (GAM) of the same institution.

Rafael Silva Freitas, Federal University of Viçosa

Graduated in Biological Sciences at the Federal University of Sergipe (UFS). He is currently a student of the Graduate Program in Plant Physiology at the Federal University of Viçosa (UFV). He has experience in the field of Botany, with an emphasis on Plant Physiology, working mainly on the following topics: Water relations, accumulation of osmotically active solutes and growth analysis of native and cultivated plants subjected to environmental stresses, with an emphasis on water deficit. Currently, he develops works analyzing hydraulic aspects in plants grown under contrasting irradiance conditions, verifying the phenotypic plasticity of these species.

Elizamar Ciríaco da Silva, Federal University of Sergipe

Graduated in Biology from the Federal Rural University of Pernambuco (1991), master's degree (2002) and doctorate (2008) in Botany from the same institution, in the area of Plant Physiology. He is currently a Level I Associate Professor at the Federal University of Sergipe, working in the field of Plant Physiology and Ecophysiology. He has experience in research on water relations, gas exchange and growth analysis of native and cultivated plants subjected to environmental stresses, with an emphasis on water and saline stresses. Currently develops projects with plants from semi-arid environments (Caatinga) and tropical fruit trees evaluating the occurrence of memory in seedlings obtained by conditioned seeds (hardening) through discontinuous hydration (hydropriming) and high temperature (thermo-priming).


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How to Cite

SANTOS JÚNIOR, J. L. dos; FREITAS, R. S.; SILVA, E. C. da. Discontinuous hydration improves germination and drought tolerance in Annona squamosa seedlings. Research, Society and Development, [S. l.], v. 10, n. 3, p. e56710313706, 2021. DOI: 10.33448/rsd-v10i3.13706. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/13706. Acesso em: 17 apr. 2021.



Agrarian and Biological Sciences