Neuroprotective action of aspirin on Paraquat intoxication in on Drosophila melanogaster

Authors

DOI:

https://doi.org/10.33448/rsd-v10i4.14179

Keywords:

Drosophila melanogaster; Aspirin; Neurodegeneration.

Abstract

Acetylsalicylic acid or aspirin is the most widely used drug globally for its anti-inflammatory characteristics, although little is known about its actions on the central nervous system (CNS). We investigated aspirin's potential neuroprotective effects against paraquat-induced neurotoxicity (PQ) in the present study. Adult male wild-type flies were exposed to a diet containing PQ (3 mM) and/or aspirin (1 μM; 5 μM; 10 μM). Flies fed with PQ reduced locomotion and increased mortality. PQ-induced neurotoxicity has also been associated with a marked decrease in acetylcholinesterase (AChE) activity and lipid peroxidation. Co-exposure to aspirin (5 μM) increased survival, improved motor performance, increased AChE activity, and decreased lipid peroxidation. Our results suggest aspirin's neuroprotective effects, probably due to its lysosomal action and antioxidant characteristics. Thus, we demonstrate that the Drosophila melanogaster model can elucidate basic aspirin mechanisms to assist the evaluations carried out in higher animals to minimize the neurodegenerative effects caused by diseases such as Parkinson's and Alzheimer's.

References

Aosaki, T., Miura, M., Suzuki, T., Nishimura, K., & Masuda, M. (2010). Acetylcholine-dopamine balance hypothesis in the striatum: An update. Geriatrics & Gerontology International, 10 Suppl 1, S148-157. https://doi.org/10.1111/j.1447-0594.2010.00588.x

Aubin, N., Curet, O., Deffois, A., & Carter, C. (1998). Aspirin and salicylate protect against MPTP-induced dopamine depletion in mice. Journal of Neurochemistry, 71(4), 1635–1642.

Ayikobua, E. T., Semuyaba, I., Eze, D. E., Kalange, M., Nansunga, M., Okpanachi, A. O., & Safiriyu, A. A. (2018, agosto 12). Combined Donepezil and Ethanolic Extract of Propolis Improved Memory Better Than Donepezil and Propolis Monotherapy in Wild Type Drosophila melanogaster [Research Article]. Evidence-Based Complementary and Alternative Medicine; Hindawi. https://doi.org/10.1155/2018/3717328

Baltazar, M. T., Dinis-Oliveira, R. J., de Lourdes Bastos, M., Tsatsakis, A. M., Duarte, J. A., & Carvalho, F. (2014). Pesticides exposure as etiological factors of Parkinson’s disease and other neurodegenerative diseases—A mechanistic approach. Toxicology Letters, 230(2), 85–103. https://doi.org/10.1016/j.toxlet.2014.01.039

Benzer, S. (1967). Behavioral mutants of Drosophila isolated by countercurrent distribution. Proceedings of the National Academy of Sciences of the United States of America, 58(3), 1112–1119. https://doi.org/10.1073/pnas.58.3.1112

Bradford, M. M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1), 248–254. https://doi.org/10.1016/0003-2697(76)90527-3

Branson, K., Robie, A., Bender, J., Perona, P., & Dickinson, M. (2009). High-throughput Ethomics in Large Groups of Drosophila. Nature methods, 6(6), 451–457. https://doi.org/10.1038/nmeth.1328

Bus, J. S., Cagen, S. Z., Olgaard, M., & Gibson, J. E. (1976). A mechanism of paraquat toxicity in mice and rats. Toxicology and Applied Pharmacology, 35(3), 501–513. https://doi.org/10.1016/0041-008X(76)90073-9

Chandra, S., Jana, M., & Pahan, K. (2018). Aspirin Induces Lysosomal Biogenesis and Attenuates Amyloid Plaque Pathology in a Mouse Model of Alzheimer’s Disease via PPARα. The Journal of Neuroscience: The Official Journal of the Society for Neuroscience, 38(30), 6682–6699. https://doi.org/10.1523/JNEUROSCI.0054-18.2018

Cheng, L., Baonza, A., & Grifoni, D. (2018). Drosophila Models of Human Disease. BioMed Research International, 2018. https://doi.org/10.1155/2018/7214974

de Oliveira Souza, A., Couto-Lima, C. A., Catalão, C. H. R., Santos-Júnior, N. N., dos Santos, J. F., da Rocha, M. J. A., & Alberici, L. C. (2019). Neuroprotective action of Eicosapentaenoic (EPA) and Docosahexaenoic (DHA) acids on Paraquat intoxication in Drosophila melanogaster. NeuroToxicology, 70, 154–160. https://doi.org/10.1016/j.neuro.2018.11.013

Erkkinen, M. G., Kim, M.-O., & Geschwind, M. D. (2018). Clinical Neurology and Epidemiology of the Major Neurodegenerative Diseases. Cold Spring Harbor Perspectives in Biology, 10(4). https://doi.org/10.1101/cshperspect.a033118

Ghosh, A., Roy, A., Liu, X., Kordower, J. H., Mufson, E. J., Hartley, D. M., Ghosh, S., Mosley, R. L., Gendelman, H. E., & Pahan, K. (2007). Selective inhibition of NF-kappaB activation prevents dopaminergic neuronal loss in a mouse model of Parkinson’s disease. Proceedings of the National Academy of Sciences of the United States of America, 104(47), 18754–18759. https://doi.org/10.1073/pnas.0704908104

Haddadi, M., Jahromi, S. R., Sagar, B. K. C., Patil, R. K., Shivanandappa, T., & Ramesh, S. R. (2014). Brain aging, memory impairment and oxidative stress: A study in Drosophila melanogaster. Behavioural Brain Research, 259, 60–69. https://doi.org/10.1016/j.bbr.2013.10.036

Hurley, E. P., & Staveley, B. E. (2021). Inhibition of Ref(2)P, the Drosophila homologue of the p62/SQSTM1 gene, increases lifespan and leads to a decline in motor function. BMC Research Notes, 14(1), 53. https://doi.org/10.1186/s13104-021-05462-6

Iqbal, K., Wang, X., Blanchard, J., Liu, F., Gong, C.-X., & Grundke-Iqbal, I. (2010). Alzheimer’s disease neurofibrillary degeneration: Pivotal and multifactorial. Biochemical Society Transactions, 38(4), 962–966. https://doi.org/10.1042/BST0380962

Jahromi, S. R., Haddadi, M., Shivanandappa, T., & Ramesh, S. R. (2013). Neuroprotective effect of Decalepis hamiltonii in paraquat-induced neurotoxicity in Drosophila melanogaster: Biochemical and behavioral evidences. Neurochemical Research, 38(12), 2616–2624. https://doi.org/10.1007/s11064-013-1179-9

Kern, S., Skoog, I., Östling, S., Kern, J., & Börjesson-Hanson, A. (2012). Does low-dose acetylsalicylic acid prevent cognitive decline in women with high cardiovascular risk? A 5-year follow-up of a non-demented population-based cohort of Swedish elderly women. BMJ Open, 2(5), e001288. https://doi.org/10.1136/bmjopen-2012-001288

Linford, N. J., Bilgir, C., Ro, J., & Pletcher, S. D. (2013). Measurement of Lifespan in Drosophila melanogaster. Journal of Visualized Experiments : JoVE, 71. https://doi.org/10.3791/50068

Livak, K. J., & Schmittgen, T. D. (2001). Analysis of relative gene expression data using real-time quantitative PCR and. Methods, 25, 402–408. https://doi.org/10.1006/meth.2001.1262

Livingston, G., Huntley, J., Sommerlad, A., Ames, D., Ballard, C., Banerjee, S., Brayne, C., Burns, A., Cohen-Mansfield, J., Cooper, C., Costafreda, S. G., Dias, A., Fox, N., Gitlin, L. N., Howard, R., Kales, H. C., Kivimäki, M., Larson, E. B., Ogunniyi, A., … Mukadam, N. (2020). Dementia prevention, intervention, and care: 2020 report of the Lancet Commission. The Lancet, 396(10248), 413–446. https://doi.org/10.1016/S0140-6736(20)30367-6

Melo, H. M. (2019). Potential Effects of Aspirin on Lysosomal Biogenesis and Amyloid-β Clearance: An Old Drug and Novel Insights in Alzheimer’s Disease Therapy. The Journal of Neuroscience, 39(2), 197–198. https://doi.org/10.1523/JNEUROSCI.2283-18.2018

Mishra, S., & Palanivelu, K. (2008). The effect of curcumin (turmeric) on Alzheimer’s disease: An overview. Annals of Indian Academy of Neurology, 11(1), 13–19. https://doi.org/10.4103/0972-2327.40220

Niveditha, S., Ramesh, S. R., & Shivanandappa, T. (2017). Paraquat-Induced Movement Disorder in Relation to Oxidative Stress-Mediated Neurodegeneration in the Brain of Drosophila melanogaster. Neurochemical Research, 42(11), 3310–3320. https://doi.org/10.1007/s11064-017-2373-y

Ohkawa, H., Ohishi, N., & Yagi, K. (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical Biochemistry, 95(2), 351–358. https://doi.org/10.1016/0003-2697(79)90738-3

Park, J. H., Jung, J. W., Ahn, Y.-J., & Kwon, H. W. (2012). Neuroprotective properties of phytochemicals against paraquat-induced oxidative stress and neurotoxicity in Drosophila melanogaster. Pesticide Biochemistry and Physiology, 104(2), 118–125. https://doi.org/10.1016/j.pestbp.2012.07.006

Riemensperger, T., Isabel, G., Coulom, H., Neuser, K., Seugnet, L., Kume, K., Iché-Torres, M., Cassar, M., Strauss, R., Preat, T., Hirsh, J., & Birman, S. (2011). Behavioral consequences of dopamine deficiency in the Drosophila central nervous system. Proceedings of the National Academy of Sciences of the United States of America, 108(2), 834–839. https://doi.org/10.1073/pnas.1010930108

Rzezniczak, T. Z., Douglas, L. A., Watterson, J. H., & Merritt, T. J. S. (2011). Paraquat administration in Drosophila for use in metabolic studies of oxidative stress. Analytical Biochemistry, 419(2), 345–347. https://doi.org/10.1016/j.ab.2011.08.023

Sereniki, A., & Vital, M. A. B. F. (2008). A doença de Alzheimer: Aspectos fisiopatológicos e farmacológicos. Revista de Psiquiatria do Rio Grande do Sul, 30(1), 0–0. https://doi.org/10.1590/S0101-81082008000200002

Shi, J., Sabbagh, M. N., & Vellas, B. (2020). Alzheimer’s disease beyond amyloid: Strategies for future therapeutic interventions. BMJ, 371, m3684. https://doi.org/10.1136/bmj.m3684

Soares, J. J., Rodrigues, D. T., Gonçalves, M. B., Lemos, M. C., Gallarreta, M. S., Bianchini, M. C., Gayer, M. C., Puntel, R. L., Roehrs, R., & Denardin, E. L. G. (2017). Paraquat exposure-induced Parkinson’s disease-like symptoms and oxidative stress in Drosophila melanogaster: Neuroprotective effect of Bougainvillea glabra Choisy. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 95, 245–251. https://doi.org/10.1016/j.biopha.2017.08.073

Song, C., Zhu, C., Wu, Q., Qi, J., Gao, Y., Zhang, Z., Gaur, U., Yang, D., Fan, X., & Yang, M. (2017). Metabolome analysis of effect of aspirin on Drosophila lifespan extension. Experimental Gerontology, 95, 54–62. https://doi.org/10.1016/j.exger.2017.04.010

Souza, A. de O., Couto-Lima, C. A., Machado, M. C. R., Espreafico, E. M., Ramos, R. G. P., & Alberici, L. (2017). Protective action of Omega-3 on paraquat intoxication in Drosophila melanogaster. Journal of toxicology and environmental health. Part A. https://doi.org/10.1080/15287394.2017.1357345

Wang, Q., Ren, N., Cai, Z., Lin, Q., Wang, Z., Zhang, Q., Wu, S., & Li, H. (2017). Paraquat and MPTP induce neurodegeneration and alteration in the expression profile of microRNAs: The role of transcription factor Nrf2. Npj Parkinson’s Disease, 3(1), 1–10. https://doi.org/10.1038/s41531-017-0033-1

Yan, D., Zhang, Y., Liu, L., & Yan, H. (2016). Pesticide exposure and risk of Alzheimer’s disease: A systematic review and meta-analysis. Scientific Reports, 6(1), 32222. https://doi.org/10.1038/srep32222

Downloads

Published

12/04/2021

How to Cite

CARMO, M. K. B. do .; FIGUEIREDO, M. O. V. .; SOUZA, J. M. de .; SOUZA, A. O. .; LIMA, C. A. C. . Neuroprotective action of aspirin on Paraquat intoxication in on Drosophila melanogaster. Research, Society and Development, [S. l.], v. 10, n. 4, p. e30710414179, 2021. DOI: 10.33448/rsd-v10i4.14179. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/14179. Acesso em: 7 may. 2021.

Issue

Section

Agrarian and Biological Sciences