Serological diagnosis of Chagas disease: Evaluation of cross reaction in patients with visceral leishmaniasis

Authors

DOI:

https://doi.org/10.33448/rsd-v10i4.14597

Keywords:

Trypanosoma cruzi; Leishmania; Serologic tests; Cross reactions.

Abstract

In Brazil, there are several simultaneously endemic regions for leishmaniasis and Chagas disease and, as the causative agents both belong to the same family, Trypanosomatidae, they share several antigens, which at the time of serological diagnosis can promote cross reactions. Several authors have evaluated Chagas/leishmaniasis cross reaction in dogs, however few studies have made this question in humans. The objective of this study was to identify the possibility of cross reaction for the diagnosis of Chagas disease in patients with cured visceral leishmaniasis. Therefore, thirty serum samples from patients with cured visceral leishmaniasis at the Infectious Diseases Clinic of the Hospital Regional de Presidente Prudente, São Paulo were analyzed. The samples were evaluated using three serological kits for the diagnosis of Chagas disease: immunoenzymatic assay (ELISA), indirect immunofluorescence (IFI) and indirect hemagglutination (HAI). Of the 30 samples analyzed, only one patient's serum showed to be reagent in the IFI, however, the duplicate evaluated was not positive, as well as the ELISA and HAI results. In the present study, due to the reactivity for the IFI to present in only one marked T. cruzi, with its duplicate and other serological tests (ELISA and HAI) negative, we can conclude that the patient with visceral leishmaniasis presented a cross reaction for the diagnosis of Chagas disease.

Author Biographies

Lucas Silva Barcelos, Universidade do Oeste Paulista

Bacharel em Biomedicina pela Universidade do Oeste Paulista, Unoeste

Amanda Aparecida Silva de Aguiar, Universidade do Oeste Paulista

Mestre em Ciências da Saúde pela Universidade do Oeste Paulista

Fernando Nunes Gavioli Boni, Universidade do Oeste Paulista

Graduando em Biomedicina pela Universidade do Oeste Paulista

Luiz Euribel Prestes Carneiro, Universidade do Oeste Paulista

Docente do Curso de Mestrado em Ciências da Saúde da Universidade do Oeste Paulista

Docente do Programa de Pós-graduação em Meio Ambiente e Desenvolvimento Regional

 

Thaís Batista de Carvalho, Universidade do Oeste Paulista

Docente da Faculdade de Ciências da Saúde da Universidade do Oeste Paulista, Unoeste

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Published

23/04/2021

How to Cite

BARCELOS, L. S. .; AGUIAR, A. A. S. de .; BONI, F. N. G. .; CARNEIRO, L. E. P. .; CARVALHO, T. B. de .; PERESI-LORDELO, E. Serological diagnosis of Chagas disease: Evaluation of cross reaction in patients with visceral leishmaniasis. Research, Society and Development, [S. l.], v. 10, n. 4, p. e55910414597, 2021. DOI: 10.33448/rsd-v10i4.14597. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/14597. Acesso em: 7 may. 2021.

Issue

Section

Health Sciences