Control of contamination and oxidation in the in vitro cultivation of olive tree (Olea Europaea L.) cv. "Koroneiki "

Abstract

The micropropagation of the olive tree is a method used to obtain several healthy seedlings in less time, compared to traditional techniques, from an explant. Thus, the objective of this work was to define a protocol of contamination and oxidation for Oliveira (Olea europaea L.) cv Koroneiki explants. For this, two experiments were performed in a completely randomized design. The first experiment was conducted in a factorial scheme (2x4), two ways of disinfesting explants with sodium hypochlorite (NaClO) (1.5% NaClO/15min, 2.5% NaClO/5min) and four cloves (Caryophyllus aromaticus L.) concentrations added to the culture medium (0.0, 0.5, 0.75 and 1.0 g L^-1) totaling 08 treatments. The second experiment was conducted with 4 treatments (T1- 0.0 g L^-1 of activated charcoal; T2 - 1.0 g L^-1 of activated charcoal, immersion and cutting of the explants in a solution of ascorbic acid at a concentration of 100 mg L^-1; T3 - 2.0 g L^-1 of activated charcoal, immersion and cutting of explants in ascorbic acid solution at a concentration of 100 mg L^-1, and T4: 2.0 g L^-1 of activated charcoal). From the results was possible to observe that the concentration of 0.5 g L^-1 of cloves in the culture medium combined with the disinfestation of the explants with 2.5% sodium hypochlorite for 5 minutes shows promising results for control of microbiological contamination of nodal segments of olive tree cv. Koroneiki. And that the use of activated charcoal in culture medium, the immersion and cutting of the explants in a solution of ascorbic acid at a concentration of 100 mg L^-1, presented favorable results in the reduction of oxidation in the explants.