Optimization of ultrasound-assisted extraction of bioactive compounds from Caryocar brasiliense
DOI:
https://doi.org/10.33448/rsd-v10i9.16493Keywords:
Leaves extracts; Box-Behnken; Phenolic compounds; Pequi.Abstract
Caryocar brasiliense, popularly known as pequi, is a species that has a high content of phenolic compounds with biological activities. In this work, the ultrasound-assisted extraction process of bioactive compounds of the pequi leaves was optimized with Box-Behnken factorial design. Four independent variables were investigated: sample concentration (liquid-solid ratio, LS), ethanol concentration (EtOH/H2O % v/v), UAE time (ultrasound-assisted extraction) and UAE temperature. The Response Surface methodology was used to point out the most important variable in the process. The results revealed the EtOH/H2O (% v/v) the most significant effect on the extraction of phenolic compounds. The optimum condition for extraction was an ethanol concentration of 60 %, UAE time of 30 min, a UAE temperature of 42 °C, and LS 18.7: 1. Under these conditions 6.30 mg GAE/g DW (n = 3) were extracted (mg gallic acid equivalent /g of dry weight). The observed experimental results confirm the suitability of the model used and its success for the optimization of extraction conditions.
References
Ahmad, A., Rehman, M. U., Wali, A. F., El-Serehy, H. A., Al-Misned, F. A., Maodaa, S. N., Aljawdah, H. M., Mir, T. M., & Ahmad, P. (2020). Box–Behnken Response Surface Design of Polysaccharide Extraction from Rhododendron arboreum and the Evaluation of Its Antioxidant Potential. Molecules, 25(17). https://doi.org/10.3390/molecules25173835
Altemimi, A. B., Mohammed, M. J., Yi-Chen, L., Watson, D. G., Lakhssassi, N., Cacciola, F., & Ibrahim, S. A. (2020). Optimization of ultrasonicated kaempferol extraction from ocimum basilicum using a box-behnken design and its densitometric validation. Foods, 9(10). https://doi.org/10.3390/foods9101379
Baptista, A., Gonçalves, R. V., Bressan, J., & do Carmo Gouveia Pelúzio, M. (2018). Antioxidant and antimicrobial activities of crude extracts and fractions of cashew (Anacardium occidentale L.), cajui (Anacardium microcarpum), and pequi (Caryocar brasiliense C.): A systematic review. Oxidative Medicine and Cellular Longevity, 2018. https://doi.org/10.1155/2018/3753562
Bemfeito, C. M., Carneiro, J. de D. S., Carvalho, E. E. N., Coli, P. C., Pereira, R. C., & Vilas Boas, E. V. de B. (2020). Nutritional and functional potential of pumpkin (Cucurbita moschata) pulp and pequi (Caryocar brasiliense Camb.) peel flours. Journal of Food Science and Technology, 57(10), 3920–3925. https://doi.org/10.1007/s13197-020-04590-4
Caldeira, A. S. P., Mbiakop, U. C., Pádua, R. M., van de Venter, M., Matsabisa, M. G., Campana, P. R. V., Cortes, S. F., & Braga, F. C. (2021). Bioguided chemical characterization of pequi (Caryocar brasiliense) fruit peels towards an anti-diabetic activity. Food Chemistry, 345(November 2020), 1–10. https://doi.org/10.1016/j.foodchem.2020.128734
de Almeida, A. B., Silva, A. K. C., Lodete, A. R., Egea, M. B., Lima, M. C. P. M., & Silva, F. G. (2019). Assessment of chemical and bioactive properties of native fruits from the Brazilian Cerrado. Nutrition and Food Science, 49(3), 381–392. https://doi.org/10.1108/NFS-07-2018-0199
de Oliveira, T. S., Thomaz, D. V., da Silva Neri, H. F., Cerqueira, L. B., Garcia, L. F., Gil, H. P. V., Pontarolo, R., Campos, F. R., Costa, E. A., dos Santos, F. C. A., de Souza Gil, E., & Ghedini, P. C. (2018). Neuroprotective Effect of Caryocar brasiliense Camb. Leaves Is Associated with Anticholinesterase and Antioxidant Properties. Oxidative Medicine and Cellular Longevity, 2018, 1–12. https://doi.org/10.1155/2018/9842908
El-Naggar, N. E. A., Hamouda, R. A., Saddiq, A. A., & Alkinani, M. H. (2021). Simultaneous bioremediation of cationic copper ions and anionic methyl orange azo dye by brown marine alga Fucus vesiculosus. Scientific Reports, 11(1), 1–19. https://doi.org/10.1038/s41598-021-82827-8
Ez Zoubi, Y., Fadil, M., Bousta, D., El Ouali Lalami, A., Lachkar, M., & Farah, A. (2021). Ultrasound-Assisted Extraction of Phenolic Compounds from Moroccan Lavandula stoechas L.: Optimization Using Response Surface Methodology. Journal of Chemistry, 2021, 1–11. https://doi.org/10.1155/2021/8830902
Fonseca, L. D., Bastos, G. A., Costa, M. A. M. S., Ferreira, A. V. de P., Silva, M. L. F., Vieira, T. M., Morais-Costa, F., Oliveira, N. J. F. de, & Duarte, E. R. (2018). Effects of Aqueous Extracts of Caryocar brasiliense in Mice. Acta Scientiae Veterinariae, 44(1), 6. https://doi.org/10.22456/1679-9216.80935
Ghitescu, R. E., Volf, I., Carausu, C., Bühlmann, A. M., Gilca, I. A., & Popa, V. I. (2015). Optimization of ultrasound-assisted extraction of polyphenols from spruce wood bark. Ultrasonics Sonochemistry, 22, 535–541. https://doi.org/10.1016/j.ultsonch.2014.07.013
Güray, T., Menevşe, B., & Yavuz, A. A. (2020). Determination of optimization parameters based on the Box-Behnken design for cloud point extraction of quinoline yellow using Brij 58 and application of this method to real samples. Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 243. https://doi.org/10.1016/j.saa.2020.118800
Jiang, Y., Wang, S., Yu, M., Wu, D., Lei, J., Li, W., He, Y., & Gang, W. (2020). Ultrasonic-Assisted Ionic Liquid Extraction of Two Biflavonoids from Selaginella tamariscina. ACS Omega, 5(51), 33113–33124. https://doi.org/10.1021/acsomega.0c04723
Junior, A. J., Leitão, M. M., Bernal, L. P. T., dos Santos, E., Kuraoka-Oliveira, Â. M., Justi, P., Argandoña, E. J. S., & Kassuya, C. A. L. (2019). Analgesic and Anti-inflammatory Effects of Caryocar brasiliense. Anti-Inflammatory & Anti-Allergy Agents in Medicinal Chemistry, 19(3), 313–322. https://doi.org/10.2174/1871523018666190408144320
Lu, C. L., Zhu, Y. F., Hu, M. M., Wang, D. M., Xu, X. J., Lu, C. J., & Zhu, W. (2015). Optimization of astilbin extraction from the rhizome of smilax glabra, and evaluation of its anti-inflammatory effect and probable underlying mechanism in lipopolysaccharide-induced raw264.7 macrophages. Molecules, 20(1), 625–644. https://doi.org/10.3390/molecules20010625
Moon, E. W., Lee, H. W., Rok, J. H., & Ha, J. H. (2020). Photocatalytic inactivation of viral particles of human norovirus by Cu-doped TiO2 non-woven fabric under UVA-LED wavelengths. Science of the Total Environment, 749. https://doi.org/10.1016/j.scitotenv.2020.141574
Nam, S., Cho, H., Han, J., Her, N., & Yoon, J. (2017). Photocatalytic degradation of acesulfame K : Optimization using the Box – Behnken design ( BBD ) Seong-Nam Nam a , c , Hyekyung Cho b , d , Jonghun Han c , Namguk Her c ,. Process Safety and Environmental Protection, 113, 10–21. https://doi.org/10.1016/j.psep.2017.09.002
Nascimento-Silva, N. R. R. D., & Naves, M. M. V. (2019). Potential of Whole Pequi (Caryocar spp.) Fruit-Pulp, Almond, Oil, and Shell-as a Medicinal Food. Journal of Medicinal Food, 22(9), 952–962. https://doi.org/10.1089/jmf.2018.0149
Nascimento-Silva, N. R. R., Mendes, N. S. R., & Silva, F. A. (2020). Nutritional composition and total phenolic compounds content of pequi pulp (Caryocar brasiliense Cambess.). Journal of Bioenergy and Food Science, 07, 1–10. https://doi.org/10.18067/jbfs.v7i2.281
Neto, J. A., Tarôco, B. R. P., dos Santos, H. B., Thomé, R. G., Wolfram, E., & Ribeiro, R. I. M. de A. (2020). Using the plants of Brazilian Cerrado for wound healing: From traditional use to scientific approach. Journal of Ethnopharmacology, 260(November 2018). https://doi.org/10.1016/j.jep.2020.112547
Oliveira, J. E., Martins, D. L., Dias, M. P. R., Treichel, T. L. E., & Prado, T. D. (2020). Avaliação macroscópica da cicatrização de feridas de pele tratadas com extrato da folha de pequizeiro (Caryocar brasiliense). Brazilian Journal of Development, 6(4), 17649–17659. https://doi.org/10.34117/bjdv6n4-075
Pires, J., Cargnin, S. T., Costa, S. A., Sinhorin, V. D. G., Damazo, A. S., Sinhorin, A. P., Bicudo, R. de C., Cavalheiro, L., Valladão, D. M. de S., Pohlmann, A. R., Guterres, S. S., & Ferrarini, S. R. (2020). Healing of dermal wounds property of Caryocar brasiliense oil loaded polymeric lipid-core nanocapsules: formulation and in vivo evaluation. European Journal of Pharmaceutical Sciences, 150(April). https://doi.org/10.1016/j.ejps.2020.105356
Prasad, K. N., Yang, E., Yi, C., Zhao, M., & Jiang, Y. (2009). Effects of high pressure extraction on the extraction yield, total phenolic content and antioxidant activity of longan fruit pericarp. Innovative Food Science and Emerging Technologies, 10(2), 155–159. https://doi.org/10.1016/j.ifset.2008.11.007
Ramos, R. de O., Pertuzatti, P. B., Gomes, I. M., Santana, M. B., Brito, R. de M., Tussolini, M., Miguel, T. B., & Tussolini, L. (2021). Chemical and antioxidant characterization, sensory and shelf-life analysis of cereal bars with almonds from pequi (Caryocar brasiliense camb.). Food Science and Technology (Brazil), 41(June), 368–374. https://doi.org/10.1590/fst.29218
Santos-Buelga, C., Gonzalez-Manzano, S., Dueñas, M., & Gonzalez-Paramas, A. M. (2012). Extraction and isolation of phenolic compounds. In Methods in Molecular Biology (Vol. 864). https://doi.org/10.1007/978-1-61779-624-1_17
Singleton, V. L., Orthofer, R., & Lamuela-Raventós, R. M. (1999). Analysis of Total Phenols and Other Oxidation Substrates and Antioxidants by Means of Folin-Ciocalteu Reagent. METHODS IN ENZYMOLOGY, 299(1974), 152–178. https://doi.org/10.1016/s0076-6879(99)99017-1
Swartz, M. R. & Krull, I. S. (1998). Validação de Métodos Cromatográficos. Pharmaceutical Technology, São Paulo, 2 (1), 12-20.
Valu, M.-V., Soare, L. C., Sutan, N. A., Ducu, C., Moga, S., Hritcu, L., Boiangiu, R. S., & Carradori, S. (2020). Optimization of Ultrasonic Extraction to Obtain Erinacine A and Polyphenols with Antioxidant Activity from the Fungal Biomass of Hericium erinaceus. Foods, 9(12), 1889. https://doi.org/10.3390/foods9121889
Vizzotto, M., & Pereira, M. C. (2011). Amora-preta (Rubus sp.): Otimização do processo de extração para determinação de compostos fenólicos antioxidantes. Revista Brasileira de Fruticultura, 33(4), 1209–1214. https://doi.org/10.1590/S0100-29452011000400020
Yuan, Z., Xu, Z., Zhang, D., Chen, W., Zhang, T., Huang, Y., Gu, L., Deng, H., & Tian, D. (2018). Box-Behnken design approach towards optimization of activated carbon synthesized by co-pyrolysis of waste polyester textiles and MgCl 2. Applied Surface Science, 427, 340–348. https://doi.org/10.1016/j.apsusc.2017.08.241
Zhang, H., Yan, H., Li, Q., Lin, H., & Wen, X. (2021). Identification of VOCs in essential oils extracted using ultrasound- and microwave-assisted methods from sweet cherry flower. Scientific Reports, 11(1), 1–13. https://doi.org/10.1038/s41598-020-80891-0
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