Analysis of pH and antimicrobial activity of a new bioceramic intracanal medicament Bio-C Temp

Authors

DOI:

https://doi.org/10.33448/rsd-v10i7.16550

Keywords:

Calcium hydroxide; Calcium silicate; Enterococcus faecalis; Antimicrobial action.

Abstract

Intracanals medications with antimicrobial activity are important as adjuvants in disinfecting the root canal system. Therefore, this study evaluated, in vitro, the pH and antimicrobial activity of a new bioceramic intracanal medication (Bio-C Temp) compared to calcium hydroxide (HC) medications. The determination of pH was carried out by means of a pH meter in the periods of 30 minutes, 3, 24, 72 and 168 hours. The medications Ultracal®XS, Calcium hydroxide with saline (HC/Saline), Calcium hydroxide with propyleneglycol (HC/PG) and Bio-C Temp (bioceramic) were tested against biofilms of two strains of Enterococcus faecalis (ATCCs 29212 and 4083) using direct contact (DCT) and membrane restricted (MRT) tests. Bacterial survival was determined by counting colony-forming units (UFCs) and the data were compared statistically (P < 0.05). The Bio-C Temp paste showed the lowest pH value in the evaluated times. The HC/Saline and HC/PG groups showed greater action compared to the control in the DCT (P < 0.05) against the two strains tested. In addition, HC/PG showed greater antibacterial activity than Bio-C Temp (P < 0.05). All groups showed a difference compared to the control group in the MRT against strain 29212, and only HC/PG showed a significant difference compared to the control against biofilms of strain 4083. Bio-C Temp showed antibacterial action similar to Ultracal®XS, however showed less action in relation to HC/PG. The calcium hydroxide paste associated with propylene glycol showed advantages over the others tested.

References

Al-Haddad, A e Che Ab Aziz, ZA (2016). Bioceramic-based root canal sealers: a review. Int J Biomater, 2016, 9753210.

Balto, H., Bukhary, S., Al-Omran, O., BaHammam, A., & Al-Mutairi, B. (2020). Combined effect of a mixture of silver nanoparticles and calcium hydroxide against Enterococcus faecalis biofilm. Journal of Endodontics, S0099-2399(20)30488-X. Advance online publication. https://doi.org/10.1016/j.joen.2020.07.001

Barros, J., Silva, M. G., Rôças, I. N., Gonçalves, L. S., Alves, F. F., Lopes, M. A., Pina-Vaz, I., & Siqueira, J. F., Jr (2014). Antibiofilm effects of endodontic sealers containing quaternary ammonium polyethylenimine nanoparticles. Journal of endodontics, 40(8), 1167–1171. https://doi.org/10.1016/j.joen.2013.12.021

Bystrom, A., Claesson, R., & Sundqvist, G. (1985). The antibacterial effect of camphorated paramonochlorophenol, camphorated phenol and calcium hydroxide in the treatment of infected root canals. Endodontics & dental traumatology, 1(5), 170–175. https://doi.org/10.1111/j.1600-9657.1985.tb00652.x

Candeiro, G. T., Correia, F. C., Duarte, M. A., Ribeiro-Siqueira, D. C., & Gavini, G. (2012). Evaluation of radiopacity, pH, release of calcium ions, and flow of a bioceramic root canal sealer. Journal of endodontics, 38(6), 842–845. https://doi.org/10.1016/j.joen.2012.02.029

Carvalho, C. N., Freire, L. G., Carvalho, A. P., Duarte, M. A., Bauer, J., & Gavini, G. (2016). Ions Release and pH of Calcium Hydroxide-, Chlorhexidine- and Bioactive Glass-Based Endodontic Medicaments. Brazilian dental journal, 27(3), 325–331. https://doi.org/10.1590/0103-6440201600602

Cheng, X., Qu, T., Ma, C., Xiang, D., Yu, Q., & Liu, X. (2017). Bioactive mono-dispersed nanospheres with long-term antibacterial effects for endodontic sealing. Journal of materials chemistry. B, 5(6), 1195–1204. https://doi.org/10.1039/C6TB02819E

Collares, F. M., Leitune, V., Portella, F. F., Santos, P. D., Balbinot, G. S., Dos Santos, L. A., Parolo, C., & Samuel, S. (2018). Methacrylate-based root canal sealer containing chlorexidine and α-tricalcium phosphate. Journal of biomedical materials research. Part B, Applied biomaterials, 106(4), 1439–1443. https://doi.org/10.1002/jbm.b.33946

de Andrade Ferreira, F. B., Silva E Souza, P., do Vale, M. S., de Moraes, I. G., & Granjeiro, J. M. (2004). Evaluation of pH levels and calcium ion release in various calcium hydroxide endodontic dressings. Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics, 97(3), 388–392. https://doi.org/10.1016/j.tripleo.2003.08.020

Estrela, C., Pécora, J. D., Souza-Neto, M. D., Estrela, C. R., & Bammann, L. L. (1999). Effect of vehicle on antimicrobial properties of calcium hydroxide pastes. Brazilian dental journal, 10(2), 63–72.

Fava, L. R., & Saunders, W. P. (1999). Calcium hydroxide pastes: classification and clinical indications. International endodontic journal, 32(4), 257–282. https://doi.org/10.1046/j.1365-2591.1999.00232.x

Ferreira, F. B., Torres, S. A., Rosa, O. P., Ferreira, C. M., Garcia, R. B., Marcucci, M. C., & Gomes, B. P. (2007). Antimicrobial effect of propolis and other substances against selected endodontic pathogens. Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics, 104(5), 709–716. https://doi.org/10.1016/j.tripleo.2007.05.019

Gao, Y., Jiang, X., Lin, D., Chen, Y., & Tong, Z. (2016). The Starvation Resistance and Biofilm Formation of Enterococcus faecalis in Coexistence with Candida albicans, Streptococcus gordonii, Actinomyces viscosus, or Lactobacillus acidophilus. Journal of endodontics, 42(8), 1233–1238. https://doi.org/10.1016/j.joen.2016.05.002.

Guerreiro, J., Ochoa-Rodrígez, V. M., Rodrigues, E. M., Chavez-Andrade, G. M., Tanomaru-Filho, M., Guerreiro-Tanomaru, J. M., & Faria, G. (2021). Antibacterial activity, cytocompatibility and effect of Bio-C Temp bioceramic intracanal medicament on osteoblast biology. International endodontic journal, 10.1111/iej.13502. Advance online publication. https://doi.org/10.1111/iej.13502

Guerreiro-Tanomaru, J. M., de Faria-Júnior, N. B., Duarte, M. A., Ordinola-Zapata, R., Graeff, M. S., & Tanomaru-Filho, M. (2013). Comparative analysis of Enterococcus faecalis biofilm formation on different substrates. Journal of endodontics, 39(3), 346–350. https://doi.org/10.1016/j.joen.2012.09.027

Kangarlou, A., Neshandar, R., Matini, N., & Dianat, O. (2016). Antibacterial efficacy of AH Plus and AH26 sealers mixed with amoxicillin, triple antibiotic paste and nanosilver. Journal of dental research, dental clinics, dental prospects, 10(4), 220–225. https://doi.org/10.15171/joddd.2016.035

Kim, D., & Kim, E. (2014). Antimicrobial effect of calcium hydroxide as an intracanal medicament in root canal treatment: a literature review - Part I. In vitro studies. Restorative dentistry & endodontics, 39(4), 241–252. https://doi.org/10.5395/rde.2014.39.4.241

Mohammadi, Z., & Dummer, P. M. (2011). Properties and applications of calcium hydroxide in endodontics and dental traumatology. International endodontic journal, 44(8), 697–730. https://doi.org/10.1111/j.1365-2591.2011.01886.x

Nakajo, K., Komori, R., Ishikawa, S., Ueno, T., Suzuki, Y., Iwami, Y., & Takahashi, N. (2006). Resistance to acidic and alkaline environments in the endodontic pathogen Enterococcus faecalis. Oral microbiology and immunology, 21(5), 283–288. https://doi.org/10.1111/j.1399-302X.2006.00289.x

Nerwich, A., Figdor, D., & Messer, H. H. (1993). pH changes in root dentin over a 4-week period following root canal dressing with calcium hydroxide. Journal of Endodontics, 19(6), 302–306. https://doi.org/10.1016/s0099-2399(06)80461-9

Oda, D. F., Duarte, M., Andrade, F. B., Moriyama, L. T., Bagnato, V. S., & de Moraes, I. G. (2019). Antimicrobial action of photodynamic therapy in root canals using LED curing light, curcumin and carbopol gel. International endodontic journal, 52(7), 1010–1019. https://doi.org/10.1111/iej.13092

Pereira, T. C., da Silva Munhoz Vasconcelos, L. R., Graeff, M., Ribeiro, M., Duarte, M., & de Andrade, F. B. (2019). Intratubular decontamination ability and physicochemical properties of calcium hydroxide pastes. Clinical oral investigations, 23(3), 1253–1262. https://doi.org/10.1007/s00784-018-2549-0

Peters, C. I., Koka, R. S., Highsmith, S., & Peters, O. A. (2005). Calcium hydroxide dressings using different preparation and application modes: density and dissolution by simulated tissue pressure. International endodontic journal, 38(12), 889–895. https://doi.org/10.1111/j.1365-2591.2005.01035.x

Rehman, K., Saunders, W. P., Foye, R. H., & Sharkey, S. W. (1996). Calcium ion diffusion from calcium hydroxide-containing materials in endodontically-treated teeth: an in vitro study. International endodontic journal, 29(4), 271–279. https://doi.org/10.1111/j.1365-2591.1996.tb01381.x

Simundi’c Muniti’c M, Poklepovi’c Peri’ci’c T, Utrobi’ci’c A, Bago I, Puljak L (2019) Antimicrobial efficacy of commercially available endodontic bioceramic root canal sealers: a systematic review. PLoS One 14, e0223575.

Swimberghe, R., Coenye, T., De Moor, R., & Meire, M. A. (2019). Biofilm model systems for root canal disinfection: a literature review. International endodontic journal, 52(5), 604–628. https://doi.org/10.1111/iej.13050

Tabrizizadeh, M., Rasti, M., Ayatollahi, F., Mossadegh, M. H., Zandi, H., Dehghan, F., & Mousavi, Z. (2015). Antimicrobial Activity of Calcium Hydroxide and Betamethasone on Enterococcus faecalis; An in vitro Assessment. Iranian endodontic journal, 10(3), 184–187. https://doi.org/10.7508/iej.2015.03.008

Tanomaru, J. M., Leonardo, M. R., Tanomaru Filho, M., Bonetti Filho, I., & Silva, L. A. (2003). Effect of different irrigation solutions and calcium hydroxide on bacterial LPS. International endodontic journal, 36(11), 733–739. https://doi.org/10.1046/j.1365-2591.2003.00717.x

Villa, N., Santos, V., Costa, U., Mendes, A. T., Duarte, P., Rosa, R., Pereira, J. R., & Só, M. (2020). A New Calcium Silicate-Based Root Canal Dressing: Physical and Chemical Properties, Cytotoxicity and Dentinal Tubule Penetration. Brazilian dental journal, 31(6), 598–604. https://doi.org/10.1590/0103-6440202003376

Wang, Z., Shen, Y., & Haapasalo, M. (2014). Dentin extends the antibacterial effect of endodontic sealers against Enterococcus faecalis biofilms. Journal of endodontics, 40(4), 505–508. https://doi.org/10.1016/j.joen.2013.10.042

Zancan, R. F., Vivan, R. R., Milanda Lopes, M. R., Weckwerth, P. H., de Andrade, F. B., Ponce, J. B., & Duarte, M. A. (2016). Antimicrobial Activity and Physicochemical Properties of Calcium Hydroxide Pastes Used as Intracanal Medication. Journal of endodontics, 42(12), 1822–1828. https://doi.org/10.1016/j.joen.2016.08.017

Zancan, R. F., Canali, L., Tartari, T., Andrade, F. B., Vivan, R. R., & Duarte, M. (2018). Do different strains of E. faecalis have the same behavior towards intracanal medications in in vitro research?. Brazilian oral research, 32, e46. https://doi.org/10.1590/1807-3107bor-2018.vol32.0046.

Zmener, O., Pameijer, C. H., & Banegas, G. (2007). An in vitro study of the pH of three calcium hydroxide dressing materials. Dental Traumatology : official publication of International Association for Dental Traumatology, 23(1), 21–25. https://doi.org/10.1111/j.1600-9657.2005.00447.x.

Published

23/06/2021

How to Cite

VIANA, F. L. P.; SAMPIERI, M. B. da S. .; VIANA, P. P.; CRUZ, S. M. L.; VIVAN, R. R. .; DUARTE, M. A. H.; ZANIN, I. C. J.; VASCONCELOS, B. C. de. Analysis of pH and antimicrobial activity of a new bioceramic intracanal medicament Bio-C Temp. Research, Society and Development, [S. l.], v. 10, n. 7, p. e33310716550, 2021. DOI: 10.33448/rsd-v10i7.16550. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/16550. Acesso em: 14 nov. 2024.

Issue

Section

Health Sciences