Potential therapy with the inhibitor of TGF-β receptors LY2109761 for oral squamous cell carcinoma

Authors

DOI:

https://doi.org/10.33448/rsd-v10i9.18396

Keywords:

Cell migration; Cytoskeleton; LY2109761; Oral squamous cell carcinoma; SCC-4.

Abstract

One way of trying to control oral squamous cell carcinoma is to invest in new therapies focused on the molecular biology of receptors and their intracellular signaling pathways. This study aimed to evaluate the effect of LY2109761 (an inhibitor of TGF-β receptors) on cell migration in oral squamous cell carcinoma in vitro. Actin cytoskeleton of SCC-4 cells control and LY2109761 (1, 5 and 10 μM) treated on three-dimensional Matrigel were analysed by using confocal laser microscopy. Control and LY2109761 (1, 5 and 10 μM) treated cells that migrated through the membrane of three-dimensional cell migration assays were counted, significance was p<0.05. Control cells were seen with voluminous cytoplasm, cell cortex preserved and actin cytoskeleton well developed with well distributed actin filaments. Regardless of concentration, cells treated showed: rounded morphology and small size, scanty cytoplasm, cortical F-actin less clear that the control cells, and disruption of actin filaments. The migratory cells were inhibited by treatment with LY2109761 [F (3, 11) = 3742, p<0.0001], in a dose-dependent manner. These results suggest that LY2109761 exerts an inhibitory effect on the actin cytoskeleton and cell migration on SCC-4 cells, therefore, it is a promising therapeutic option for oral squamous cell carcinoma.

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Published

01/08/2021

How to Cite

REZENDE, A. S.; CARNEIRO, A. C. D. M. .; SILVA, B. R. O. .; CARBONI, S. de S. C. M. .; CREMA, V. O. Potential therapy with the inhibitor of TGF-β receptors LY2109761 for oral squamous cell carcinoma. Research, Society and Development, [S. l.], v. 10, n. 9, p. e54810918396, 2021. DOI: 10.33448/rsd-v10i9.18396. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/18396. Acesso em: 25 sep. 2021.

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Health Sciences