Effect of tribulus terrestris extract on cardiac histomorphology and testicular physiology of rats





Testosterone; Heart; Weight gain; Testis.


Tribulus terrestris (TT) is a plant native to India, known as Abrolo or “Puncture Vine”. There are many uncertainties related to the effects of TT on the athlete's physical power, testosterone levels and as an aphrodisiac. There are few reports on the effect of TT on the cardiovascular system, however they mention that there may be a negative correlation between TT and the activity of the angiotensin-converting enzyme. In this study we evaluated the effect of TT without weight gain on serum testosterone concentration and cardiac morphometry in rats. Three rats were randomly divided into 2 experimental groups (n = 15): Group CT, treated with 0.9% saline solution; TT group, treated with Tribulus terrestris extract. After 60 days of treatment, animals that received TT gained less body weight and did not dissipate serum testosterone levels and cardiac morphometry when compared to rats in the CT group. Our results obtained that TT prevented weight gain without altering the energy balance of the diet, without altering testosterone secretion and without altering cardiac morphometry. Therefore, TT may have a promising future in preserving body weight, making preventing weight gain easier to achieve than losing weight and maintaining that loss.


Antonio, J., Uelmen, J., Rodriguez, R., & Earnest, C. (2000). The effects of Tribulus terrestris on body composition and exercise performance in resistance-trained males. International journal of sport nutrition and exercise metabolism, 10(2), 208–215. https://doi.org/10.1123/ijsnem.10.2.208.

Arcasoy, H. B., Erenmemisoglu, A., Tekol, Y., Kurucu, S., & Kartal, M. (1998). Effect of Tribulus terrestris L. saponin mixture on some smooth muscle preparations: a preliminary study. Bollettino chimico farmaceutico, 137(11), 473–475.

Armstrong, L. E., Soto, J. A., Hacker, F. T., Jr, Casa, D. J., Kavouras, S. A., & Maresh, C. M. (1998). Urinary indices during dehydration, exercise, and rehydration. International journal of sport nutrition, 8(4), 345–355. https://doi.org/10.1123/ijsn.8.4.345.

Asadmobini, A., Bakhtiari, M., Khaleghi, S., Esmaeili, F., & Mostafaei, A. (2017). The effect of Tribulus terrestris extract on motility and viability of human sperms after cryopreservation. Cryobiology, 75, 154–159. https://doi.org/10.1016/j.cryobiol.2017.02.005.

Brown, G. A., Vukovich, M. D., Reifenrath, T. A., Uhl, N. L., Parsons, K. A., Sharp, R. L., & King, D. S. (2000). Effects of anabolic precursors on serum testosterone concentrations and adaptations to resistance training in young men. International journal of sport nutrition and exercise metabolism, 10(3), 340–359. https://doi.org/10.1123/ijsnem.10.3.340.

Chhatre, S., Nesari, T., Somani, G., Kanchan, D., & Sathaye, S. (2014). Phytopharmacological overview of Tribulus terrestris. Pharmacognosy reviews, 8(15), 45–51. https://doi.org/10.4103/0973-7847.125530.

Do, J., Choi, S., Choi, J., & Hyun, J. S. (2013). Effects and Mechanism of Action of a Tribulus terrestris Extract on Penile Erection. Korean journal of urology, 54(3), 183–188. https://doi.org/10.4111/kju.2013.54.3.183.

GamalEl Din, S. F., Abdel Salam, M. A., Mohamed, M. S., Ahmed, A. R., Motawaa, A. T., Saadeldin, O. A., & Elnabarway, R. R. (2019). Tribulus terrestris versus placebo in the treatment of erectile dysfunction and lower urinary tract symptoms in patients with late-onset hypogonadism: A placebo-controlled study. Urologia, 86(2), 74–78. https://doi.org/10.1177/0391560318802160.

Gauthaman, K., & Ganesan, A. P. (2008). The hormonal effects of Tribulus terrestris and its role in the management of male erectile dysfunction--an evaluation using primates, rabbit and rat. Phytomedicine: international journal of phytotherapy and phytopharmacology, 15(1-2), 44–54. https://doi.org/10.1016/j.phymed.2007.11.011.

Gauthaman, K., Adaikan, P. G., & Prasad, R. N. (2002). Aphrodisiac properties of Tribulus Terrestris extract (Protodioscin) in normal and castrated rats. Life sciences, 71(12), 1385–1396. https://doi.org/10.1016/s0024-3205(02)01858-1.

Guo, Y., Shi, D. Z., Yin, H. J., & Chen, K. J. (2007). Effects of Tribuli saponins on ventricular remodeling after myocardial infarction in hyperlipidemic rats. The American journal of Chinese medicine, 35(2), 309–316. https://doi.org/10.1142/S0192415X07004837.

Hussain A., Abbas A. Mohammed, Heba. H. Ibrahim, & Amir H. Abbas. (2009). Study the Biological Activities of Tribulus Terrestris Extracts. 3:9-24. http://doi.org/10.5281/zenodo.1085151.

Junqueira, L. C., Bignolas, G., & Brentani, R. R. (1979). Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections. The Histochemical journal, 11(4), 447–455. https://doi.org/10.1007/BF01002772.

Khaleghi, S., Bakhtiari, M., Asadmobini, A., & Esmaeili, F. (2017). Tribulus terrestris Extract Improves Human Sperm Parameters In Vitro. Journal of evidence-based complementary & alternative medicine, 22(3), 407–412. https://doi.org/10.1177/2156587216668110.

Kohut, M. L., Thompson J. R., Campbell J., Brown G. A., Vukovich M. D., Jackson D. A., & King D. S. (2003). Ingestion of a dietary supplement containing dehydroepiandrosterone (DHEA) and androstenedione has minimal effect on immune function in middle-aged men. Journal of the American College of Nutrition. 22:363–371. https://doi.org/10.1080/07315724.2003.10719319.

Lemos Júnior L. P. H., Lemos A. L. A., Lemos L. M. D. (2011). Tribulus terrestris. Diagn Tratamento. 16:170-173.

Ma, Y., Guo, Z., & Wang, X. (2017). Tribulus terrestris extracts alleviate muscle damage and promote anaerobic performance of trained male boxers and its mechanisms: Roles of androgen, IGF-1, and IGF binding protein-3. Journal of sport and health science, 6(4), 474–481. https://doi.org/10.1016/j.jshs.2015.12.003.

Malviya, N., Jain, S., Gupta, V. B., & Vyas, S. (2011). Recent studies on aphrodisiac herbs for the management of male sexual dysfunction--a review. Acta poloniae pharmaceutica, 68(1), 3–8.

Malviya, N., Malviya, S., Jain, S., & Vyas, S. (2016). A review of the potential of medicinal plants in the management and treatment of male sexual dysfunction. Andrologia, 48(8), 880–893. https://doi.org/10.1111/and.12677.

Neychev, V., & Mitev, V. (2016). Pro-sexual and androgen enhancing effects of Tribulus terrestris L.: Fact or Fiction. Journal of ethnopharmacology, 179, 345–355. https://doi.org/10.1016/j.jep.2015.12.055.

Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa científica. UFSM. https://repositorio.ufsm.br/bitstream/ handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.

Pokrywka, A., Obmiński, Z., Malczewska-Lenczowska, J., Fijałek, Z., Turek-Lepa, E., & Grucza, R. (2014). Insights into Supplements with Tribulus Terrestris used by Athletes. Journal of human kinetics, 41, 99–105. https://doi.org/10.2478/hukin-2014-0037.

Prathapan, A., Vineetha, V. P., & Raghu, K. G. (2014). Protective effect of Boerhaavia diffusa L. against mitochondrial dysfunction in angiotensin II induced hypertrophy in H9c2 cardiomyoblast cells. PloS one, 9(4), e96220. https://doi.org/10.1371/journal.pone.0096220.

Prescott, H., & Khan, I. (2020). Medicinal plants/herbal supplements as female aphrodisiacs: Does any evidence exist to support their inclusion or potential in the treatment of FSD? Journal of ethnopharmacology, 251, 112464. https://doi.org/10.1016/j.jep.2019.112464.

Qureshi, A., Naughton, D. P., & Petroczi, A. (2014). A systematic review on the herbal extract Tribulus terrestris and the roots of its putative aphrodisiac and performance enhancing effect. Journal of dietary supplements, 11(1), 64–79. https://doi.org/10.3109/19390211.2014.887602.

Reshma, P. L., Binu, P., Anupama, N., Vineetha, R. C., Abhilash, S., Nair, R. H., & Raghu, K. G. (2019). Pretreatment of Tribulus terrestris L. causes anti-ischemic cardioprotection through MAPK mediated anti-apoptotic pathway in rat. Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie, 111, 1342-1352. https://doi.org/10.1016/j.biopha.2019.01.033.

Reshma, P. L., Lekshmi, V. S., Sankar, V., & Raghu, K. G. (2015). Tribulus terrestris (Linn.) Attenuates Cellular Alterations Induced by Ischemia in H9c2 Cells Via Antioxidant Potential. Phytotherapy research: PTR, 29(6), 933–943. https://doi.org/10.1002/ptr.5336.

Reshma, P. L., Sainu, N. S., Mathew, A. K., & Raghu, K. G. (2016). Mitochondrial dysfunction in H9c2 cells during ischemia and amelioration with Tribulus terrestris L. Life sciences, 152, 220–230. https://doi.org/10.1016/j.lfs.2016.03.055.

Roaiah, M. F., Elkhayat, Y. I., Abd El Salam, M. A., & Din, S. (2017). Prospective Analysis on the Effect of Botanical Medicine (Tribulus terrestris) on Serum Testosterone Level and Semen Parameters in Males with Unexplained Infertility. Journal of dietary supplements, 14(1), 25–31. https://doi.org/10.1080/19390211.2016.1188193.

Rogerson, S., Riches, C. J., Jennings, C., Weatherby, R. P., Meir, R. A., & Marshall-Gradisnik, S. M. (2007). The effect of five weeks of Tribulus terrestris supplementation on muscle strength and body composition during preseason training in elite rugby league players. Journal of strength and conditioning research, 21(2), 348–353. https://doi.org/10.1519/R-18395.1.

Salgado, R. M., Marques-Silva, M. H., Gonçalves, E., Mathias, A. C., Aguiar, J. G., & Wolff, P. (2017). Effect of oral administration of Tribulus terrestris extract on semen quality and body fat index of infertile men. Andrologia, 49(5), 10.1111/and.12655. https://doi.org/10.1111/and.12655.

Samaha, A. A., Nasser-Eddine, W., Shatila, E., Haddad, J. J., Wazne, J., & Eid, A. H. (2008). Multi-organ damage induced by anabolic steroid supplements: a case report and literature review. Journal of medical case reports, 2, 340. https://doi.org/10.1186/1752-1947-2-340.

Samani, N. B., Jokar, A., Soveid, M., Heydari, M., & Mosavat, S. H. (2016). Efficacy of the Hydroalcoholic Extract of Tribulus terrestris on the Serum Glucose and Lipid Profile of Women With Diabetes Mellitus: A Double-Blind Randomized Placebo-Controlled Clinical Trial. Journal of evidence-based complementary & alternative medicine, 21(4), NP91–NP97. https://doi.org/10.1177/2156587216650775.

Sanagoo, S., Sadeghzadeh Oskouei, B., Gassab Abdollahi, N., Salehi-Pourmehr, H., Hazhir, N., & Farshbaf-Khalili, A. (2019). Effect of Tribulus terrestris L. on sperm parameters in men with idiopathic infertility: A systematic review. Complementary therapies in medicine, 42, 95–103. https://doi.org/10.1016/j.ctim.2018.09.015.

Sankar, V., Pangayarselvi, B., Prathapan, A., & Raghu, K. G. (2013). Desmodium gangeticum (Linn.) DC. exhibits antihypertrophic effect in isoproterenol-induced cardiomyoblasts via amelioration of oxidative stress and mitochondrial alterations. Journal of cardiovascular pharmacology, 61(1), 23–34. https://doi.org/10.1097/FJC.0b013e3182756ad3.

Santos, H. O., Howell, S., & Teixeira, F. J. (2019). Beyond tribulus (Tribulus terrestris L.): The effects of phytotherapics on testosterone, sperm and prostate parameters. Journal of Ethnopharmacology, 235, 392-405. https://doi.org/10.1016/j.jep.2019.02.033.

Shahid, M., Riaz, M., Talpur, M. M., & Pirzada, T. (2016). Phytopharmacology of Tribulus terrestris. Journal of biological regulators and homeostatic agents, 30(3), 785–788.

Shirani, J., Pick, R., Roberts, W. C., & Maron, B. J. (2000). Morphology and significance of the left ventricular collagen network in young patients with hypertrophic cardiomyopathy and sudden cardiac death. Journal of the American College of Cardiology, 35(1), 36–44. https://doi.org/10.1016/s0735-1097(99)00492-1.

Souza, A. da S., Ribeiro Junior, O. C., Ribeiro, J. S. S., Mendonça, L. B., Melo, J. M. R. de, & Araújo, T. S. de. (2020). The use of phytotherapy in the management of women in climacteric/menopause. Research, Society and Development, 9(9), e415997416. https://doi.org/10.33448/rsd-v9i9.7416.

Ștefănescu, R., Tero-Vescan, A., Negroiu, A., Aurică, E., & Vari, C. E. (2020). A Comprehensive Review of the Phytochemical, Pharmacological, and Toxicological Properties of Tribulus terrestris L. Biomolecules, 10(5), 752. https://doi.org/10.3390/biom10050752.

Wang, B., Ma, L., & Liu, T. (1990). Zhong xi yi jie he za zhi = Chinese journal of modern developments in traditional medicine, 10(2), 85–68.

Wu, Y., Yang, H., & Wang, X. (2017). The function of androgen/androgen receptor and insulin growth factor 1/insulin growth factor 1 receptor on the effects of Tribulus terrestris extracts in rats undergoing high intensity exercise. Molecular medicine reports, 16(3), 2931–2938. https://doi.org/10.3892/mmr.2017.6891.

Yin, L., Wang, Q., Wang, X., & Song, L. N. (2016). Effects of Tribulus terrestris saponins on exercise performance in overtraining rats and the underlying mechanisms. Canadian journal of physiology and pharmacology, 94(11), 1193–1201. https://doi.org/10.1139/cjpp-2016-0086.

Yuan, Z., Du, W., He, X., Zhang, D., & He, W. (2020). Tribulus terrestris Ameliorates Oxidative Stress-Induced ARPE-19 Cell Injury through the PI3K/Akt-Nrf2 Signaling Pathway. Oxidative medicine and cellular longevity, 2020, 7962393. https://doi.org/10.1155/2020/7962393.

Zhang, H., Tong, W. T., Zhang, C. R., Li, J. L., Meng, H., Yang, H. G., & Chen, M. (2019). Gross saponin of Tribulus terrestris improves erectile dysfunction in type 2 diabetic rats by repairing the endothelial function of the penile corpus cavernosum. Diabetes, metabolic syndrome and obesity: targets and therapy, 12, 1705–1716. https://doi.org/10.2147/DMSO.S205722.



How to Cite

LUCIANO, L. L. .; SOARES, E. A. .; LIMA, S. J. F. de .; GRIEBELER , R. T. .; FORGERINI , R. .; PEREIRA , M. .; CAIXETA, E. .; BOAVENTURA, V. L. .; CUNHA, D. F. da .; BARROS, G. B. S.; GARCIA, J. A. D. . Effect of tribulus terrestris extract on cardiac histomorphology and testicular physiology of rats. Research, Society and Development, [S. l.], v. 10, n. 10, p. e180101018634, 2021. DOI: 10.33448/rsd-v10i10.18634. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/18634. Acesso em: 18 oct. 2021.



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