Cinnamaldehyde and α-terpineol inhibit the growth of planktonic cultures of Candida albicans and non albicans
DOI:
https://doi.org/10.33448/rsd-v10i10.19027Keywords:
Candida albicans; Candida glabrata; Candida krusei; Oral candidiasis; Phytotherap.Abstract
Agents based in natural products have been investigated for the treatment of oral candidiasis. This study aims to evaluate the antifungal effect of phytoconstituent cinnamaldehyde and α-terpineol in planktonic cultures of Candida albicans, Candida glabrata, Candida krusei and clinical isolates of C. albicans. Reference strains of C. albicans (ATCC 90028 and ATCC 60193), C. glabrata (ATCC 2001), C. krusei (ATCC 34135) and four clinical isolates were used. Nistatin 100,000UI was used as a positive control. After preparation of the inoculum (1 × 103 CFU / mL), serial microdilution technique was performed using RPMI 1640 medium. Results: in reference strains, the MIC for α-terpineol ranged from 312,5 μg / mL (C. albicans 90028) to 40 μg / mL (C. krusei); and the cinnamaldehyde ranged from 40 μg / mL (C. albicans 90028, C. albicans 60193 and C. glabrata) to 20 μg / mL (C. krusei). Whereas for clinical strains, the MIC for α-terpineol ranged from 156 μg / mL to 78 μg / mL and cinnamaldehyde ranged from 78 μg / mL to 40 μg / mL. Therefore, the cinnamaldehyde and α-terpineol present an inhibitory effect against planktonic cultures of Candida albicans and not albicans.
References
Akers, K. S. et al. (2015). Biofilm formation by clinical isolates and its relevance to clinical infections. Adv Exp Med Biol. 830:1-28. 10.1007/978-3-319-11038-7_1.
Bakhtiari, S. et al. (2019). The Effects of Cinnamaldehyde (Cinnamon Derivatives) and Nystatin on Candida Albicans and Candida Glabrata. Open Access Maced J Med Sci. 10(7):1067-1070. 10.3889/oamjms.2019.245.
Cavalcanti, Y. W. et al. (2015). Virulence and pathogenicity of Candida albicans is enhanced in biofilms containing oral bacteria. Biofouling. 31(1):27-38. 10.1080/08927014.2014.996143.
Cavalcanti, Y. W. et al. (2016). Modulation of Candida albicans virulence by bacterial biofilms on titanium surfaces. Biofouling. 32(2):123-34. 10.1080/08927014.2015.1125472.
Clinical and Laboratory Standards Institute (CLSI). (2008). Reference method for broth dilution antifungal susceptibility testing of yeasts. (3rd ed). Wayne: Clinical and Laboratory Standards Institute.
Chu, J. et al. (2016). Cariogenicity features of Streptococcus mutans in presence of rubusoside. BMC Oral Health. 16(1):54. 10.1186/s12903-016-0212-1.
Dias, I. J. et al. (2018). Antifungal activity of linalool in cases of Candida spp. isolated from individuals with oral candidiasis. Braz J Biol. 78(2):368-374. 10.1590/1519-6984.171054.
Dogan, G. et al. (2017). Chemical composition and biological activities of leaf and fruit essential oils from Eucalyptus camaldulensis. Z Naturforsch C J Biosci. 72(11-12):483-489. 10.1515/znc-2016-0033.
Ferreira, G. L. et al. (2015). Does scientific evidence for the use of natural products in the treatment of oral candidiasis exist? A systematic review. Evid Based Complement Alternat Med. 2015:147804. 10.1155/2015/147804.
Franco, J. et al. (2005). Composição química e atividade antimicrobiana in vitro do óleo essencial de Eucalyptus cinerea F. Mull. ex Benth., Myrtaceae, extraído em diferentes intervalos de tempo. Rev Brasi de Farmacognosia. 15:191-194.
Ferro, T. A. et al. (2016). Cinnamaldehyde Inhibits Staphylococcus aureus Virulence Factors and Protects against Infection in a Galleria mellonella Model. Front Microbiol. 21(7):2052. 10.3389/fmicb.2016.02052.
Gulati, M. et al. (2016). Candida albicans biofilms: development, regulation, and molecular mechanisms. Microbes Infect. 18(5):310-21. 10.1016/j.micinf.2016.01.002.
Hassan, S. B. et al. (2010) Alpha terpineol: a potential anticancer agent which acts through suppressing NF-kappaB signalling. Anticancer Res. 30(6):1911-9.
Hellstein, J. W. et al. (2019). Candidiasis: Red and White Manifestations in the Oral Cavity. Head Neck Pathol. 13(1):25-32. 10.1007/s12105-019-01004-6.
Holetz, F. B. et al. (2002). Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Mem Inst Oswaldo Cruz. 97(7):1027-31.
Hu, L. et al. (2019). Characterization of oral candidiasis and the Candida species profile in patients with oral mucosal diseases. Microb Pathog. 134:103575. 10.1016/j.micpath.2019.103575.
Jørgensen, M. R. et al. (2017). Probiotic Lactobacillus reuteri has antifungal effects on oral Candida species in vitro. J Oral Microbiol. (1):1274582. 10.1080/20002297.2016.1274582.
Kabwe, M. et al. (2016). Etiology, Antibiotic Resistance and Risk Factors for Neonatal Sepsis in a Large Referral Center in Zambia. Pediatr Infect Dis J. 35(7):191-8. 10.1097/INF.0000000000001154.
Kong, Q. et al. (2019). Antifungal mechanisms of α-terpineol and terpene-4-alcohol as the critical components of Melaleuca alternifolia oil in the inhibition of rot disease caused by Aspergillus ochraceus in postharvest grapes. J Appl Microbiol. 2019 Apr;126(4):1161-1174. 10.1111/jam.14193.
Lyu, X. et al. (2016). Efficacy of nystatin for the treatment of oral candidiasis: a systematic review and meta-analysis. Drug Des Devel Ther. 16(10):1161-71. 10.2147/DDDT.S100795.
Nogueira, M. N. et al. (2014). Terpinen-4-ol and alpha-terpineol (tea tree oil components) inhibit the production of IL-1β, IL-6 and IL-10 on human macrophages. Inflamm Res. 2014 Sep;63(9):769-78. 10.1007/s00011-014-0749-x.
Park, S. N. (2012). Antimicrobial effect of linalool and α-terpineol against periodontopathic and cariogenic bacteria. Anaerobe. 18(3):369-72. 10.1016/j.anaerobe.2012.04.001.
Perlin, D. S. (2017). Rautemaa-Richardson R, Alastruey-Izquierdo A. The global problem of antifungal resistance: prevalence, mechanisms, and management. Lancet Infect Dis. 17(12):383-392. 10.1016/S1473-3099(17)30316-X.
Polke, M. et al. (2017). Quorum sensing by farnesol revisited. Curr Genet 63(5):791-797. 10.1007/s00294-017-0683-x..
Pootong, A. et al. (2017). Antifungal activity of cinnamaldehyde against Candida albicans. Southeast Asian J Trop Med Public Health. 48(1):150-8.
Quindós, G. et al. (2019). Therapeutic tools for oral candidiasis: Current and new antifungal drugs. Med Oral Patol Oral Cir Bucal. 24(2):172-180. 10.4317/medoral.22978.
Radović, K. et al. (2014). Denture stomatitis and salivary vascular endothelial growth factor in immediate complete denture wearers with type 2 diabetes. J Prosthet Dent. 111(5):373-9. 10.1016/j.prosdent.2013.07.019.
Ramage, G. et al. (2012). Antifungal, cytotoxic, and immunomodulatory properties of tea tree oil and its derivative components: potential role in management of oral candidosis in cancer patients. Front Microbiol. 18;3:220. 10.3389/fmicb.2012.00220.
Sardi, J. C. et al. (2013). Candida species: current epidemiology, pathogenicity, biofilm formation, natural antifungal products and new therapeutic options. J Med Microbiol. 62(1):10-24. 10.1099/jmm.0.045054-0.
Silva, S. et al. (2012). Candida glabrata, Candida parapsilosis and Candida tropicalis: biology, epidemiology, pathogenicity and antifungal resistance. FEMS Microbiol Rev. 36(2):288-305. 10.1111/j.1574-6976.2011.00278.x.
Suryana, K. et al. (2020). Factors Associated with Oral Candidiasis in People Living with HIV/AIDS: A Case Control Study. HIV AIDS (Auckl). 14(12):33-39. 10.2147/HIV.S236304..
Taguchi, Y. et al. (2013). The effect of cinnamaldehyde on the growth and the morphology of Candida albicans. Med Mol Morphol. 46(1):8-13. 10.1007/s00795-012-0001-0.
Taguchi, Y. et al. (2012). Effect of cinnamaldehyde on hyphal growth of C. albicans under various treatment conditions. Med Mycol J. 53(3):199-204.
Trentin, M. S. (2017). Most Frequent Oral Lesions in Patients with Type 2 Diabetes Mellitus. J Contemp Dent Pract. 18(2):107-111.
Trinh, H. T. et al. (2011). Artemisia princeps Pamp. Essential oil and its constituents eucalyptol and α-terpineol ameliorate bacterial vaginosis and vulvovaginal candidiasis in mice by inhibiting bacterial growth and NF-κB activation. Planta Med. 77(18):1996-2002. 10.1055/s-0031-1280094.
Wu, Z. et al. (2018). Administration of cinnamaldehyde promotes osteogenesis in ovariectomized rats and differentiation of osteoblast in vitro. J Pharmacol Sci. 138(1):63-70. 10.1016/j.jphs.2018.09.002.
Zhang, C. et al. (2019). Alpha-Terpineol production from an engineered Saccharomyces cerevisiae cell factory. Microb Cell Fact. 18(1):160. 10.1186/s12934-019-1211-0.
Zhang, L. W. et al. (2016). Efficacy and safety of miconazole for oral candidiasis: a systematic review and meta-analysis. Oral Dis. 22(3):185-95. 10.1111/odi.12380.
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