Male Swiss mice (Mus musculus) as a most suitable experimental model for the study of Giardia duodenalis BIV




Balb/c; C57BL/6; Experimental model; Giardia duodenalis; Giardiasis.


In this study, we proposed to verify the most suitable murine experimental model for studying human giardiasis. In total 150 animals were used. Fifty mice (Mus musculus) from each lineage (Swiss, Balb/c and C57BL/6), 25 females and 25 males, were divided into 5 groups with 5 animals each, according to the lineage/sex. Three groups were infected with 104 cysts of Giardia duodenalis of assemblage BIV and 2 negative control groups. The animals were followed and evaluated for 15 days after receiving the inoculum. The clinical parameters evaluated were body weight, water and feed intake, excretion, appearance of fur and feces, elimination of Giardia spp cysts and behavioral assessment. The clinical parameters of the groups infected with G. duodenalis were compared with the non-infected groups within their own lineage/sex. In the 15 days of monitoring, only the male Swiss mice presented differences in these parameters. The infected animals consumed more feed, water and eliminated more excreta than the non-infected group. There was no difference in the general average of the weight of the animals or in the behavioral assessment in any group. Only the infected male Swiss mice eliminated G. duodenalis cysts in the feces, which was confirmed by the molecular diagnosis and by observing the presence of trophozoites in the intestinal mucosa. The results demonstrate that the most suitable animal model for the study of human giardiasis is the male Swiss mice, since it was the only one capable of developing infection by G. duodenalis cysts.


Almeida, C. R., Bezagio, R. C., Colli, C. M., Romera, L. I. L. & Gomes, M. L. (2021). Elimination of Giardia muris in naturally infected murine experimental model: Complementary treatment. Research, Society and Development, 10(7):e60010716996.

Astiazarán-Garcia, H., Espinosa-Cantellano, M., Castañón, G., Chavéz-Munguía, B. & Martínez-Palomo, A. (2000). Giardia lamblia: effect of infection with symptomatic and asymptomatic isolates on growth of gerbils (Meriones unguiculatus). Experimental Parasitology, 95:128-135.

Baker, D. G. (2006). Parasitic diseases. In: Suckow MA, Weisbroth SH, Franklin CL (ed) The laboratory rat. Elsevier, 453-478.

Baker, J. M. & Alonso, W. J. (2018). Rotavirus vaccination takes seasonal signature of childhood diarrhea back to pre-sanitation era in Brazil. Journal of Infection, 76:68–77.

Baltert, L. A., Roche, J., Kolling, G., Bolick, D., Noronha, F., Naylor, C., Hoffman, P, Warren, C., Singer, S. & Guerrant, R. (2013). Persistent G. lamblia impairs growth in a murine malnutrition model. The Journal of Clinical Investigation, 123:2672–2684.

Bezagio, R. C., Colli, C. M., Romera, L. I. L., Almeida, C. R., Ferreira, É. C., Mattia, S., & Gomes, M. L. (2020). Improvement in cyst recovery and molecular detection of Giardia duodenalis from stool samples. Molecular Biology Reports, 47(2), 1233–1239.

Bezagio, R. C., Colli, C. M., Romera, L. I. L., Ferreira, É. C., Falavigna-Guilherme, A. L. & Gomes, M. L. (2017). Synergistic effects of fenbendazole and metronidazole against Giardia muris in Swiss mice naturally infected. Parasitology Research, 116:939- 944. 10.1007/s00436-016-5367-9

Bicalho, K. A., Araujo, F. T. M., Rocha, R. S. & Carvalho, O. (2007). Perfil sanitário de colônias de camundongos e ratos de biotérios de Minas Gerais: I - Endo e ectoparasitos. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 59:1478-1484.

Buret, A., Gall, D. G. & Olson, M. E. (1991). Growth, activities of enzymes in the small intestines and ultrastructure of microvillous border in gerbils infected with Giardia duodenalis. Parasitology Research, 77:109-114.

Cacciò, S. M. & Ryan, U. (2008). Molecular epidemiology of giardiasis. Molecular and Biochemical Parasitology, 160:75–80.

Chorilli, M., Michelin, D. C. & Salgado, H. R. N. (2007). Animais de laboratório: o camundongo. Revista de Ciências Farmacêuticas Básica e Aplicada, 28:1123.

Cock, I. E. & Rayan, P. (2020). Ascorbic acid potentiates the Giardia duodenalis growth inhibitory activity of pure Terminalia ferdinandiana Exell compounds. Parasitology Research.

Colli, C. M., Bezagio, R. C., Nishi, L., Bignotto, T. S., Ferreira, É. C., Falavigna-Guilherme, A. L. & Gomes, M. L. (2015). Identical Assemblage of Giardia duodenalis in Humans, Animals and Vegetables in an Urban Area in Southern Brazil Indicates a Relationship among Them. Plos One, 10:e0118065.

Dressen, L., Bosscher, K., Grit, G., Staels, B., Lubberts, E., Bauge, E. & Geldhof, P. (2014). Giardia muris infection in mice is associated with a protective interleukin 17A response and induction of peroxisome proliferator activated receptor alpha. Infection and Immunology, 82:3333-3340.

Ehret, T., Torelli, F., Klotz, C., Pedersen, A. B. & Seeber, F. (2017). Translational Rodent Models for Research on Parasitic Protozoa - A Review of Confounders and Possibilities. Frontiers in Cellular and Infection Microbiology, 7:238.

Fantinatti, M., Bello, A. R., Fernandes, O. & Da-Cruz, A. M. (2016). Identification of Giardia lamblia Assemblage E in Humans Points to a New Anthropozoonotic Cycle. Journal of Infectious Diseases, 214:1256–1259.

Faust, E. C., D´Antoni, J. S., Odom, V., Miller, M. J., Peres, C., Sawitz, W., Thomen, I. F., Tobie, J. & Walker, J. H. (1938). A critical study of clinical laboratory technics for the diagnosis of protozoan cysts and helminth eggs in feces. The American Journal of Tropical Medicine and Hygiene, 18:169-183.

Feng, Y. & Xiao, L. (2011). Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. Clinical Microbiology Reviews, 24:110–140.

Guénet, J. L. (2011). Animal models of human genetic disesases: do they need to be faithful to be useful? Molecular Genetics and Genomics, 286:1-20.

Goyal, N., Rishi, P. & Shukla, G. (2013). Lactobacillus rhamnosus GG antagonizes Giardia intestinalis induced oxidative stress and intestinal disaccharidases: an experimental study. World Journal of Microbiology and Biotechnology, 29:1049-1057.

Hooshyar, H., Rostamkhani, P., Arbabi, M. & Delavari, M. (2019). Giardia lamblia infection: review of current diagnostic strategies. Gastroenterology and Hepatology from Bed to Bench, 12:3-12.

Lasek-Nesselquist, E., Welch, D. M. & Sogin, M. L. (2010). The identification of a new Giardia duodenalis assemblage in marine vertebrates and a preliminar analysis of G. duodenalis population biology in marine system. International Journal for Parasitology, 40:1063–1074. 10.1016/j.ijpara.2010.02.015

Lebbad, M., Mattsson, J. G., Christensson, B., Ljungström, B., Backhans, A., Andersson, J. O. & Svärd, S. G. (2010). From mouse to moose: multilocus genotyping of Giardia isolates from various animal species. Veterinary Parasitology, 168:231–239.

Lemée, V., Zaharia, I., Nevez, G., Rabodonirina, M., Brasseur, P., Ballet, J. J. & Favannec, L. (2000). Metronidazole and albendazole susceptibility of 11 clinical isolates of Giardia duodenalis from France. Journal of Antimicrobial Chemotherapy, 46:819–821.

Li, E., Liu, M. & Singer, S. M. (2014). Resistance to reinfection in mice as a vaccine model for giardiasis. Human Vaccines & Immunotherapeutics, 10:1536-1543.

Lima, A. A. M., Oliveira, D. B., Quetz, J. S., Havt, A., Prata, M. M. G., Lima, I. F. N., Soares, A. M., Filho, J. Q., Lima, N. L., Medeiros, P. H. Q. S., Santos, A. K. S., Veras, H. N., Gondim, R. N. D. G., Pankov, R. C., Bona, M. D., Rodrigues, F. A. P., Moreira, R. A., Moreira, A. C. O. M., Bertolini, M., Bertolini, L. R., Freitas, V. J. F., Houpt, E. R. & Guerrant, R. L. (2019). Etiology and severity of diarrheal diseases in infants at the semiarid region of Brazil: A case-control study. PLoS Neglected Tropical Diseases, 13:e0007154.

Massironi, S. M. G. (2009). Padrão genético. In: Lapchik VBV, Mattaraia VGM, Ko GM (ed) Cuidados e Manejo de Animais de Laboratório, (2rd ed.) Editora Atheneu, 385 – 398.

Matsuchita, H. L. P., Pitz, A. F., Melanda, F. N., Bregano, R. M., Oliveira, F. J. A., Mori, F. M. R. L., Menezes, M. C. N. D., Costa, I. N., Pavanelli, W. R., Gomes, M. L., Colli, C. M., Venancio, E. J. & Conchon-Costa, I. (2017). Descriptive molecular epidemiology study of Giardia duodenalis in children of Parana State, Brazil. International Journal of Epidemiology Research, 4:1–9.

Mayol, G. F., Revuelta, M. V., Salusso, A., Touz, M. C. & Rópolo, A. S. (2019). Evidence of nuclear transport mechanisms in the protozoan parasite Giardia lamblia. Biochimica et biophysica acta - Molecular Cell Research, 1867:118566.

Mizutani, H., Tamagawa-Mineoka, R., Minami, Y., Yagita, K. & Katoh, N. (2017). Constant light exposure impairs immune tolerance development in mice. Journal of Dermatological Science, 86:63–70.

Monis, P. T., Andrews, R. H., Mayrhofer, G. & Ey, P. L. (1999). Molecular systematics of the parasitic protozoan Giardia intestinalis. Molecular Biology Evolution, 16:1135-1144.

Nakada, L. Y. K., Franco, R. M. B., Fiuza, V. R. S., Santos, L. U., Branco, N. & Guimarães, J. R. (2018). Pre-ozonation of source water: assessment of efficacy against Giardia duodenalis cysts and effects on natural organic matter. Chemosphere.

Pavanelli, M. F., Colli, C. M., Gomes, M. L., Góis, M. B., Alcântara-Nogueira, G. M., Almeida-Araújo, E. J. & de Gonçales-Sant’Ana, D. M. (2018). Comparative study of effects of assemblages AII and BIV of Giardia duodenalis on mucosa and microbiota of the small intestine in mice. Biomedicine & Pharmacotherapy, 101:563–571.

Qi, M., Ji, X., Zhang, Y., Wei, Z., Jing, B., Zhang, L., Lin, X., Karim, M. R., Wang, H. & Sun, M. (2020). Prevalence and multilocus analysis of Giardia duodenalis in racehorses in China. Parasitology Research.

Scott, K. G. E., Logan, M. R., Klammer, G. M., Teoh, D. A. & Buret, A. G. (2000). Jejunal brush border microvillous alterations in Giardia muris - infected mice: role of T lymphocytes and interleukin-6. Infection and Immunity, 68:3412-3418. 10.1128/ iai.68.6.3412-3418.2000

Soares, J. F., Silva, A. S., Oliveira, C. B., Silva, M. K., Mariscano, G., Salomão, E. L. & Monteiro, S. G. (2008). Parasitismo por Giardia sp. e Cryptosporidium sp. em Coendou villosus. Ciência Rural, 38:548–550.

Solaymani-Mohammadi, S. & Singer, S. M. (2010). Giardia duodenalis: the double-edged sword of immune responses in Giardiasis. Experimental Parasitology, 126:292–297.

Strober, W. (2015). Trypan Blue Exclusion Test of Cell Viability. Current Protocols in Immunology.

Thompson, R. C. A., Hopkins, R. M. & Homan, W. L. (2000). Nomenclature and genetic groupings of Giardia infecting mammals. Parasitology Today, 16:210-213.

Uda-Shimoda, C. F., Colli, C. M., Pavanelli, M. F., Falavigna-Guilherme, A. L. & Gomes, M. L. (2014). Simplified protocol for DNA extraction and amplification of 2 molecular markers to detect and type Giardia duodenalis. Diagnostic Microbiology and Infectious Disease, 78(1):53-58.

Vaidya, H. B., Gangadaran, S. & Cheema, S. K. (2017). A high fat-high sucrose diet enriched in blue mussels protects against systemic inflammation, metabolic dysregulation and weight gain in C57BL/6 mice. Food Research International, 100:78–85.

Von-Allmen, N., Christen, S., Forster, U., Gottstein, B., Welle, M. & Muller, N. (2006). Acute trichinellosis increases susceptibility to Giardia lamblia infection in the mouse model. Parasitology, 133:139-149.

Zhao, Z., Wang, R., Zhao, W., Qi, M., Zhao, J., Zhang, L., Li, J. & Liu, A. (2015). Genotyping and subtyping of Giardia and Cryptosporidium isolates from commensal rodents in China. Parasitology, 142:800–806.

Ware, M. W. & Villegas, E. N. (2019). Propagation of Giardia duodenalis cysts in immunosuppressed CF-1 mice. Veterinary Parasitology, 268:32-35.




How to Cite

ROMERA, L. I. L.; BEZAGIO, R. C.; FERREIRA, W. C.; ALMEIDA, C. R. de; GOMES, M. L. . Male Swiss mice (Mus musculus) as a most suitable experimental model for the study of Giardia duodenalis BIV . Research, Society and Development, [S. l.], v. 10, n. 10, p. e493101019250, 2021. DOI: 10.33448/rsd-v10i10.19250. Disponível em: Acesso em: 29 feb. 2024.



Health Sciences